Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79221.t1 | XP_003601959.2 | 79.9 | 199 | 40 | 0 | 1 | 199 | 1 | 199 | 3.80E-88 | 334.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79221.t1 | G7J3S5 | 79.9 | 199 | 40 | 0 | 1 | 199 | 1 | 199 | 2.7e-88 | 334.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene79221 | 0.844227 | 8.62E-59 | -1.69E-46 |
| MS.gene050329 | MS.gene79221 | 0.803346 | 3.64E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene79221 | 0.800278 | 1.55E-48 | -1.69E-46 |
| MS.gene051844 | MS.gene79221 | 0.809183 | 2.15E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene79221 | 0.869168 | 3.79E-66 | -1.69E-46 |
| MS.gene052212 | MS.gene79221 | 0.817248 | 3.64E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene79221 | 0.841394 | 4.88E-58 | -1.69E-46 |
| MS.gene053403 | MS.gene79221 | 0.806645 | 7.44E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene79221 | 0.823624 | 1.25E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene79221 | 0.803039 | 4.22E-49 | -1.69E-46 |
| MS.gene053887 | MS.gene79221 | 0.812539 | 4.03E-51 | -1.69E-46 |
| MS.gene054146 | MS.gene79221 | 0.842489 | 2.51E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene79221 | 0.819243 | 1.29E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene79221 | 0.874893 | 4.73E-68 | -1.69E-46 |
| MS.gene056218 | MS.gene79221 | 0.816052 | 6.74E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene79221 | 0.813829 | 2.10E-51 | -1.69E-46 |
| MS.gene056298 | MS.gene79221 | 0.810025 | 1.42E-50 | -1.69E-46 |
| MS.gene056590 | MS.gene79221 | 0.816641 | 4.98E-52 | -1.69E-46 |
| MS.gene056591 | MS.gene79221 | 0.816306 | 5.92E-52 | -1.69E-46 |
| MS.gene056748 | MS.gene79221 | 0.847353 | 1.22E-59 | -1.69E-46 |
| MS.gene057768 | MS.gene79221 | 0.810541 | 1.09E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene79221 | 0.834254 | 3.33E-56 | -1.69E-46 |
| MS.gene059955 | MS.gene79221 | 0.81643 | 5.55E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene79221 | 0.808256 | 3.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79221.t1 | MTR_3g087270 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 6.68e-134 | 373 |
| MS.gene79221.t1 | MTR_3g087280 | 87.940 | 199 | 24 | 0 | 1 | 199 | 1 | 199 | 7.80e-133 | 370 |
| MS.gene79221.t1 | MTR_3g087278 | 89.447 | 199 | 21 | 0 | 1 | 199 | 1 | 199 | 2.29e-132 | 369 |
| MS.gene79221.t1 | MTR_3g087325 | 88.945 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 3.71e-131 | 366 |
| MS.gene79221.t1 | MTR_3g087275 | 88.945 | 199 | 21 | 1 | 1 | 199 | 1 | 198 | 1.66e-129 | 362 |
| MS.gene79221.t1 | MTR_3g087330 | 85.930 | 199 | 28 | 0 | 1 | 199 | 1 | 199 | 1.10e-128 | 360 |
| MS.gene79221.t1 | MTR_3g087300 | 67.005 | 197 | 21 | 3 | 1 | 197 | 1 | 153 | 7.81e-87 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79221.t1 | AT5G65030 | 30.612 | 196 | 113 | 6 | 11 | 187 | 10 | 201 | 8.00e-24 | 94.0 |
| MS.gene79221.t1 | AT5G10210 | 30.769 | 195 | 109 | 7 | 15 | 196 | 13 | 194 | 2.34e-23 | 92.4 |
Find 43 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCAAATCAGTGCCCTTT+TGG | 0.235029 | 3.1:-70243816 | MS.gene79221:CDS |
| CTCATGCCCTCAAGAGTTCT+TGG | 0.272286 | 3.1:-70243770 | MS.gene79221:CDS |
| ATAAGAGGAGAATGAATAAT+TGG | 0.280000 | 3.1:-70243501 | MS.gene79221:CDS |
| TTAATCTCCACTTCCACTTC+TGG | 0.281665 | 3.1:+70243562 | None:intergenic |
| ATGTTGGGGTCACAACTTAT+TGG | 0.325437 | 3.1:-70243700 | MS.gene79221:CDS |
| CCACTAGGCTTTGTGTGTTA+AGG | 0.346972 | 3.1:+70243743 | None:intergenic |
| TGTTGGGGTCACAACTTATT+GGG | 0.372157 | 3.1:-70243699 | MS.gene79221:CDS |
| ATTTGCGCTTGGTGCAGCTT+TGG | 0.382032 | 3.1:-70243413 | MS.gene79221:CDS |
| ATCCCAAAAGGGCACTGATT+TGG | 0.392569 | 3.1:+70243813 | None:intergenic |
| TGCGCTTGGTGCAGCTTTGG+AGG | 0.432752 | 3.1:-70243410 | MS.gene79221:CDS |
| ATTAAAAGACTCATCCCAAA+AGG | 0.445229 | 3.1:+70243801 | None:intergenic |
| GCAAGGCTGTATCTTCCAAC+AGG | 0.447411 | 3.1:-70243877 | MS.gene79221:CDS |
| GATTCAAGAATCACCTTCCA+TGG | 0.467787 | 3.1:+70243664 | None:intergenic |
| AAAATGGTTGAAGATGGATT+TGG | 0.470551 | 3.1:-70243623 | MS.gene79221:CDS |
| AGTAGCTTCGGTTGCTGAGA+TGG | 0.471131 | 3.1:-70243533 | MS.gene79221:CDS |
| GATTAAAATAAAAGTAGCTT+CGG | 0.474275 | 3.1:-70243545 | MS.gene79221:CDS |
| CTAAGGCAGAGGAAGATGTT+GGG | 0.479580 | 3.1:-70243715 | MS.gene79221:CDS |
| CCAAAAGGGCACTGATTTGG+TGG | 0.481239 | 3.1:+70243816 | None:intergenic |
| AGTGGAAGTGATTGCAAAGA+GGG | 0.484026 | 3.1:-70243598 | MS.gene79221:CDS |
| TTGGGAAAGGTGAGATTCCA+TGG | 0.488562 | 3.1:-70243681 | MS.gene79221:CDS |
| AGATGGAGAAGCAAAATAAG+AGG | 0.509817 | 3.1:-70243516 | MS.gene79221:CDS |
| CAACTGAAGTTTGCATATGC+AGG | 0.509989 | 3.1:+70243961 | None:intergenic |
| GCTAAGGCAGAGGAAGATGT+TGG | 0.514746 | 3.1:-70243716 | MS.gene79221:CDS |
| TCTGCCTTAGCTCCACCACT+AGG | 0.532067 | 3.1:+70243728 | None:intergenic |
| ACGGCCATGACCATCGTGCT+TGG | 0.538014 | 3.1:-70243459 | MS.gene79221:CDS |
| AAAGCCTAGTGGTGGAGCTA+AGG | 0.543884 | 3.1:-70243732 | MS.gene79221:CDS |
| TTAAAAGACTCATCCCAAAA+GGG | 0.557578 | 3.1:+70243802 | None:intergenic |
| ATGAATAATTGGAATGAGTG+TGG | 0.557798 | 3.1:-70243490 | MS.gene79221:CDS |
| GGGTCACAACTTATTGGGAA+AGG | 0.564555 | 3.1:-70243694 | MS.gene79221:CDS |
| AGCACCAAGCACGATGGTCA+TGG | 0.566327 | 3.1:+70243455 | None:intergenic |
| CATAACGCCAGAAGTGGAAG+TGG | 0.570407 | 3.1:-70243569 | MS.gene79221:CDS |
| TAGTGGTGGAGCTAAGGCAG+AGG | 0.576496 | 3.1:-70243726 | MS.gene79221:CDS |
| GATTGTGATATATTTGCGCT+TGG | 0.580894 | 3.1:-70243424 | MS.gene79221:CDS |
| TATGTTCATAACGCCAGAAG+TGG | 0.583659 | 3.1:-70243575 | MS.gene79221:CDS |
| GATTCTTGAATCACAAAACA+TGG | 0.589945 | 3.1:-70243653 | MS.gene79221:CDS |
| TTGAAGATGGATTTGGTGAG+TGG | 0.598106 | 3.1:-70243616 | MS.gene79221:CDS |
| GAAAGGTGAGATTCCATGGA+AGG | 0.610143 | 3.1:-70243677 | MS.gene79221:CDS |
| TAAGGCAGAGGAAGATGTTG+GGG | 0.620231 | 3.1:-70243714 | MS.gene79221:CDS |
| TAACACACAAAGCCTAGTGG+TGG | 0.644101 | 3.1:-70243740 | MS.gene79221:CDS |
| AAACGTTGAATTCATCAAGT+CGG | 0.660973 | 3.1:-70243914 | MS.gene79221:CDS |
| CAGCGCAGCACCAAGCACGA+TGG | 0.681591 | 3.1:+70243449 | None:intergenic |
| CCTTAACACACAAAGCCTAG+TGG | 0.691369 | 3.1:-70243743 | MS.gene79221:CDS |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.703550 | 3.1:-70243599 | MS.gene79221:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACATGGAGTTAAAAAAA+TGG | - | chr3.1:70243742-70243761 | MS.gene79221:CDS | 20.0% |
| !! | GATTAAAATAAAAGTAGCTT+CGG | - | chr3.1:70243836-70243855 | MS.gene79221:CDS | 20.0% |
| ! | ATAAGAGGAGAATGAATAAT+TGG | - | chr3.1:70243880-70243899 | MS.gene79221:CDS | 25.0% |
| ! | GTTAAAAAAATGGTTGAAGA+TGG | - | chr3.1:70243752-70243771 | MS.gene79221:CDS | 25.0% |
| AAACGTTGAATTCATCAAGT+CGG | - | chr3.1:70243467-70243486 | MS.gene79221:CDS | 30.0% | |
| ATGAATAATTGGAATGAGTG+TGG | - | chr3.1:70243891-70243910 | MS.gene79221:CDS | 30.0% | |
| ATTAAAAGACTCATCCCAAA+AGG | + | chr3.1:70243583-70243602 | None:intergenic | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | chr3.1:70243728-70243747 | MS.gene79221:CDS | 30.0% | |
| TTAAAAGACTCATCCCAAAA+GGG | + | chr3.1:70243582-70243601 | None:intergenic | 30.0% | |
| ! | AAAATGGTTGAAGATGGATT+TGG | - | chr3.1:70243758-70243777 | MS.gene79221:CDS | 30.0% |
| !! | ATTCTTTTCTTGTTTCCTGT+TGG | + | chr3.1:70243522-70243541 | None:intergenic | 30.0% |
| AATGAGTGTGGATGCAAAAA+CGG | - | chr3.1:70243903-70243922 | MS.gene79221:CDS | 35.0% | |
| AGATGGAGAAGCAAAATAAG+AGG | - | chr3.1:70243865-70243884 | MS.gene79221:CDS | 35.0% | |
| GATTGTGATATATTTGCGCT+TGG | - | chr3.1:70243957-70243976 | MS.gene79221:CDS | 35.0% | |
| AGTGGAAGTGATTGCAAAGA+GGG | - | chr3.1:70243783-70243802 | MS.gene79221:CDS | 40.0% | |
| ATGTTGGGGTCACAACTTAT+TGG | - | chr3.1:70243681-70243700 | MS.gene79221:CDS | 40.0% | |
| CAACTGAAGTTTGCATATGC+AGG | + | chr3.1:70243423-70243442 | None:intergenic | 40.0% | |
| CGGCAAAAAACATGTTTGCA+AGG | - | chr3.1:70243487-70243506 | MS.gene79221:CDS | 40.0% | |
| GATTCAAGAATCACCTTCCA+TGG | + | chr3.1:70243720-70243739 | None:intergenic | 40.0% | |
| TATGTTCATAACGCCAGAAG+TGG | - | chr3.1:70243806-70243825 | MS.gene79221:CDS | 40.0% | |
| TGTTGGGGTCACAACTTATT+GGG | - | chr3.1:70243682-70243701 | MS.gene79221:CDS | 40.0% | |
| TTAATCTCCACTTCCACTTC+TGG | + | chr3.1:70243822-70243841 | None:intergenic | 40.0% | |
| ! | AGGTTTTCCAAGAACTCTTG+AGG | + | chr3.1:70243621-70243640 | None:intergenic | 40.0% |
| ! | GGTTTTCCAAGAACTCTTGA+GGG | + | chr3.1:70243620-70243639 | None:intergenic | 40.0% |
| !! | TTGAAGATGGATTTGGTGAG+TGG | - | chr3.1:70243765-70243784 | MS.gene79221:CDS | 40.0% |
| CCTTAACACACAAAGCCTAG+TGG | - | chr3.1:70243638-70243657 | MS.gene79221:CDS | 45.0% | |
| CTAAGGCAGAGGAAGATGTT+GGG | - | chr3.1:70243666-70243685 | MS.gene79221:CDS | 45.0% | |
| GAAAGGTGAGATTCCATGGA+AGG | - | chr3.1:70243704-70243723 | MS.gene79221:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | chr3.1:70243782-70243801 | MS.gene79221:CDS | 45.0% | |
| GGGTCACAACTTATTGGGAA+AGG | - | chr3.1:70243687-70243706 | MS.gene79221:CDS | 45.0% | |
| TAACACACAAAGCCTAGTGG+TGG | - | chr3.1:70243641-70243660 | MS.gene79221:CDS | 45.0% | |
| TAAGGCAGAGGAAGATGTTG+GGG | - | chr3.1:70243667-70243686 | MS.gene79221:CDS | 45.0% | |
| TTGGGAAAGGTGAGATTCCA+TGG | - | chr3.1:70243700-70243719 | MS.gene79221:CDS | 45.0% | |
| ! | CACCAAATCAGTGCCCTTTT+GGG | - | chr3.1:70243566-70243585 | MS.gene79221:CDS | 45.0% |
| ! | CCACTAGGCTTTGTGTGTTA+AGG | + | chr3.1:70243641-70243660 | None:intergenic | 45.0% |
| ! | GCATATGCAGGTGTTTTCGA+TGG | + | chr3.1:70243411-70243430 | None:intergenic | 45.0% |
| !! | ATCCCAAAAGGGCACTGATT+TGG | + | chr3.1:70243571-70243590 | None:intergenic | 45.0% |
| AGTAGCTTCGGTTGCTGAGA+TGG | - | chr3.1:70243848-70243867 | MS.gene79221:CDS | 50.0% | |
| CATAACGCCAGAAGTGGAAG+TGG | - | chr3.1:70243812-70243831 | MS.gene79221:CDS | 50.0% | |
| CTCATGCCCTCAAGAGTTCT+TGG | - | chr3.1:70243611-70243630 | MS.gene79221:CDS | 50.0% | |
| GCAAGGCTGTATCTTCCAAC+AGG | - | chr3.1:70243504-70243523 | MS.gene79221:CDS | 50.0% | |
| GCTAAGGCAGAGGAAGATGT+TGG | - | chr3.1:70243665-70243684 | MS.gene79221:CDS | 50.0% | |
| ! | AAAGCCTAGTGGTGGAGCTA+AGG | - | chr3.1:70243649-70243668 | MS.gene79221:CDS | 50.0% |
| ! | CCACCAAATCAGTGCCCTTT+TGG | - | chr3.1:70243565-70243584 | MS.gene79221:CDS | 50.0% |
| !! | ATTTGCGCTTGGTGCAGCTT+TGG | - | chr3.1:70243968-70243987 | MS.gene79221:CDS | 50.0% |
| !! | CCAAAAGGGCACTGATTTGG+TGG | + | chr3.1:70243568-70243587 | None:intergenic | 50.0% |
| AGCACCAAGCACGATGGTCA+TGG | + | chr3.1:70243929-70243948 | None:intergenic | 55.0% | |
| TCTGCCTTAGCTCCACCACT+AGG | + | chr3.1:70243656-70243675 | None:intergenic | 55.0% | |
| ! | TAGTGGTGGAGCTAAGGCAG+AGG | - | chr3.1:70243655-70243674 | MS.gene79221:CDS | 55.0% |
| ACGGCCATGACCATCGTGCT+TGG | - | chr3.1:70243922-70243941 | MS.gene79221:CDS | 60.0% | |
| !! | TGCGCTTGGTGCAGCTTTGG+AGG | - | chr3.1:70243971-70243990 | MS.gene79221:CDS | 60.0% |
| CAGCGCAGCACCAAGCACGA+TGG | + | chr3.1:70243935-70243954 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 70243402 | 70244001 | 70243402 | ID=MS.gene79221 |
| chr3.1 | mRNA | 70243402 | 70244001 | 70243402 | ID=MS.gene79221.t1;Parent=MS.gene79221 |
| chr3.1 | exon | 70243402 | 70244001 | 70243402 | ID=MS.gene79221.t1.exon1;Parent=MS.gene79221.t1 |
| chr3.1 | CDS | 70243402 | 70244001 | 70243402 | ID=cds.MS.gene79221.t1;Parent=MS.gene79221.t1 |
| Gene Sequence |
| Protein sequence |
