AlfalfaGEDB | MS.gene79713

M. sativa cultivar XinJiangDaYe / MS.gene79713

KEGG: K03236
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003743 GO:0005488 GO:0006413 GO:0008135 GO:0008150 GO:0008152 GO:0009987 GO:0044237 GO:0045182 GO:0090079 GO:0097159 GO:1901363
PFAM: PF01176.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79713.t1	XP_013444714.1	98.6	146	2	0	1	146	1	146	1.30E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79713.t1	P56331	90.5	147	11	2	1	146	1	145	6.7e-67	254.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79713.t1	G7ZYQ6	98.6	146	2	0	1	146	1	146	9.6e-75	288.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene79713	0.804997	1.65E-49	-1.69E-46
MS.gene049861	MS.gene79713	0.814624	1.40E-51	-1.69E-46
MS.gene049983	MS.gene79713	0.811045	8.52E-51	-1.69E-46
MS.gene050654	MS.gene79713	0.819137	1.36E-52	-1.69E-46
MS.gene050941	MS.gene79713	0.802428	5.64E-49	-1.69E-46
MS.gene051003	MS.gene79713	0.813069	3.08E-51	-1.69E-46
MS.gene051184	MS.gene79713	0.802768	4.80E-49	-1.69E-46
MS.gene051597	MS.gene79713	0.834345	3.16E-56	-1.69E-46
MS.gene05188	MS.gene79713	0.804861	1.76E-49	-1.69E-46
MS.gene051975	MS.gene79713	0.834402	3.06E-56	-1.69E-46
MS.gene052434	MS.gene79713	0.805814	1.11E-49	-1.69E-46
MS.gene053058	MS.gene79713	0.823359	1.45E-53	-1.69E-46
MS.gene053456	MS.gene79713	0.801512	8.70E-49	-1.69E-46
MS.gene053726	MS.gene79713	0.80339	3.57E-49	-1.69E-46
MS.gene053931	MS.gene79713	0.819515	1.12E-52	-1.69E-46
MS.gene056605	MS.gene79713	0.811246	7.70E-51	-1.69E-46
MS.gene056986	MS.gene79713	0.83435	3.15E-56	-1.69E-46
MS.gene057102	MS.gene79713	0.806045	9.95E-50	-1.69E-46
MS.gene057208	MS.gene79713	0.804006	2.66E-49	-1.69E-46
MS.gene05897	MS.gene79713	0.800591	1.34E-48	-1.69E-46
MS.gene059146	MS.gene79713	0.821545	3.81E-53	-1.69E-46
MS.gene059830	MS.gene79713	0.800017	1.76E-48	-1.69E-46
MS.gene059901	MS.gene79713	-0.802021	6.84E-49	-1.69E-46
MS.gene06010	MS.gene79713	0.814847	1.25E-51	-1.69E-46
MS.gene06011	MS.gene79713	0.832349	9.95E-56	-1.69E-46
MS.gene060586	MS.gene79713	0.808474	3.04E-50	-1.69E-46
MS.gene060763	MS.gene79713	0.806164	9.39E-50	-1.69E-46
MS.gene060935	MS.gene79713	0.807881	4.07E-50	-1.69E-46
MS.gene060937	MS.gene79713	0.812362	4.40E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79713.t1	MTR_8g028180	98.630	146	2	0	1	146	1	146	3.24e-101	286
MS.gene79713.t1	MTR_3g005520	83.333	150	21	1	1	146	1	150	2.32e-77	226

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene79713.t1	AT2G04520	87.671	146	17	1	1	146	1	145	8.27e-85	245
MS.gene79713.t1	AT5G35680	86.986	146	18	1	1	146	1	145	7.68e-80	232
MS.gene79713.t1	AT5G35680	86.986	146	18	1	1	146	1	145	7.68e-80	232
MS.gene79713.t1	AT5G35680	93.277	119	8	0	1	119	1	119	6.39e-78	228

Find 47 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGGGTTGCGTGATTATC+AGG	0.308178	8.4:+67777229	MS.gene79713:CDS
TCATTAAGCCTCACACCTTC+AGG	0.319525	8.4:-67777323	None:intergenic
CCTCCCTTTCCCTTGTTCTT+CGG	0.331257	8.4:-67776999	None:intergenic
GCTGGGGATATTATTCTTGT+TGG	0.331915	8.4:+67777212	MS.gene79713:CDS
GGAATTGGTGGTGGGATTGA+TGG	0.360212	8.4:+67777347	MS.gene79713:CDS
CTGGGGATATTATTCTTGTT+GGG	0.363705	8.4:+67777213	MS.gene79713:CDS
GAGGATATTGATAAGATTTG+AGG	0.399236	8.4:+67777416	MS.gene79713:CDS
GAAAGATGCACAAGAAGGTT+TGG	0.408452	8.4:+67777183	MS.gene79713:CDS
CTTTCCCTTGTTCTTCGGCA+TGG	0.425552	8.4:-67776994	None:intergenic
GAATTGGTGGTGGGATTGAT+GGG	0.434528	8.4:+67777348	MS.gene79713:CDS
AAGAAGGTTTGGATTGCTGC+TGG	0.450047	8.4:+67777194	MS.gene79713:CDS
TTATATTGAGTTTGAGGATG+AGG	0.463979	8.4:+67777397	MS.gene79713:CDS
GCACAGGTTCTTCGTATGCT+TGG	0.465282	8.4:+67777101	MS.gene79713:CDS
GGGGATGATGATGAGGGTGA+TGG	0.471229	8.4:+67777368	MS.gene79713:CDS
GGGATGATGATGAGGGTGAT+GGG	0.477444	8.4:+67777369	MS.gene79713:CDS
TTCAACAATCTCGCCTCATC+CGG	0.477469	8.4:-67777284	None:intergenic
AGGATATTGATAAGATTTGA+GGG	0.478420	8.4:+67777417	MS.gene79713:CDS
GAATGATTATATTGAGTTTG+AGG	0.491210	8.4:+67777391	MS.gene79713:CDS
GTGAGGCTTAATGAAGGAAT+TGG	0.513375	8.4:+67777332	MS.gene79713:CDS
CTTAATGAAGGAATTGGTGG+TGG	0.513624	8.4:+67777338	MS.gene79713:CDS
GAAGGTTTGGATTGCTGCTG+GGG	0.517248	8.4:+67777196	MS.gene79713:CDS
GCGTGATTATCAGGATGATA+AGG	0.518535	8.4:+67777238	MS.gene79713:CDS
AGAAGGTTTGGATTGCTGCT+GGG	0.519846	8.4:+67777195	MS.gene79713:CDS
CCTTCTTGTGCATCTTTCCA+CGG	0.526928	8.4:-67777178	None:intergenic
GGATGAGGCGAGATTGTTGA+AGG	0.537010	8.4:+67777286	MS.gene79713:CDS
ATGCCGAAGAACAAGGGAAA+GGG	0.538255	8.4:+67776996	MS.gene79713:CDS
GATTGATGGGGATGATGATG+AGG	0.545936	8.4:+67777361	MS.gene79713:CDS
TTCAACCATGCCGAAGAACA+AGG	0.546102	8.4:+67776989	None:intergenic
GTTCTTCGTATGCTTGGAAA+CGG	0.546331	8.4:+67777107	MS.gene79713:CDS
CTTGAAGTATATGCCGGATG+AGG	0.557673	8.4:+67777271	MS.gene79713:CDS
ATTGATGGGGATGATGATGA+GGG	0.568262	8.4:+67777362	MS.gene79713:CDS
CTTTGTTCCATCGATACACA+TGG	0.573074	8.4:-67777141	None:intergenic
GGAGGAAAGAATCGCAAGAG+AGG	0.578624	8.4:+67777017	MS.gene79713:CDS
CCGTGGAAAGATGCACAAGA+AGG	0.600822	8.4:+67777178	MS.gene79713:CDS
CATGCCGAAGAACAAGGGAA+AGG	0.602323	8.4:+67776995	None:intergenic
TTAATGAAGGAATTGGTGGT+GGG	0.613970	8.4:+67777339	MS.gene79713:CDS
AAGCGTCTCTGTCATATCCG+TGG	0.616715	8.4:+67777161	MS.gene79713:CDS
GCGTATAGCGAGCTTCCTGA+AGG	0.618073	8.4:+67777308	MS.gene79713:CDS
GAAGGTGTGAGGCTTAATGA+AGG	0.621479	8.4:+67777326	MS.gene79713:CDS
TGTGAAGCCATGTGTATCGA+TGG	0.627821	8.4:+67777134	MS.gene79713:CDS
CCGAAGAACAAGGGAAAGGG+AGG	0.634335	8.4:+67776999	MS.gene79713:CDS
AATTGGTGGTGGGATTGATG+GGG	0.634571	8.4:+67777349	MS.gene79713:CDS
AGGCTTAATGAAGGAATTGG+TGG	0.634691	8.4:+67777335	MS.gene79713:CDS
TCAACCATGCCGAAGAACAA+GGG	0.635918	8.4:+67776990	None:intergenic
GCGAGCTTCCTGAAGGTGTG+AGG	0.643888	8.4:+67777315	MS.gene79713:CDS
AGATGGTCAAGAATACGCAC+AGG	0.647023	8.4:+67777085	MS.gene79713:CDS
TGTGATCTTGAAGTATATGC+CGG	0.651550	8.4:+67777265	MS.gene79713:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GAATGATTATATTGAGTTTG+AGG	+	chr8.4:67777391-67777410	MS.gene79713:CDS	25.0%
	TTATATTGAGTTTGAGGATG+AGG	+	chr8.4:67777397-67777416	MS.gene79713:CDS	30.0%
	CTGGGGATATTATTCTTGTT+GGG	+	chr8.4:67777213-67777232	MS.gene79713:CDS	35.0%
	TGTGATCTTGAAGTATATGC+CGG	+	chr8.4:67777265-67777284	MS.gene79713:CDS	35.0%
!	TTAATGAAGGAATTGGTGGT+GGG	+	chr8.4:67777339-67777358	MS.gene79713:CDS	35.0%
	AGGCTTAATGAAGGAATTGG+TGG	+	chr8.4:67777335-67777354	MS.gene79713:CDS	40.0%
	CTTAATGAAGGAATTGGTGG+TGG	+	chr8.4:67777338-67777357	MS.gene79713:CDS	40.0%
	CTTTGTTCCATCGATACACA+TGG	-	chr8.4:67777144-67777163	None:intergenic	40.0%
	GAAAGATGCACAAGAAGGTT+TGG	+	chr8.4:67777183-67777202	MS.gene79713:CDS	40.0%
	GCGTGATTATCAGGATGATA+AGG	+	chr8.4:67777238-67777257	MS.gene79713:CDS	40.0%
	GCTGGGGATATTATTCTTGT+TGG	+	chr8.4:67777212-67777231	MS.gene79713:CDS	40.0%
	GTGAGGCTTAATGAAGGAAT+TGG	+	chr8.4:67777332-67777351	MS.gene79713:CDS	40.0%
	GTTCTTCGTATGCTTGGAAA+CGG	+	chr8.4:67777107-67777126	MS.gene79713:CDS	40.0%
!	ATTGATGGGGATGATGATGA+GGG	+	chr8.4:67777362-67777381	MS.gene79713:CDS	40.0%
!!	AAAGCGTGAGCTTGTTTTCA+AGG	+	chr8.4:67777061-67777080	MS.gene79713:CDS	40.0%
!!	GAGCTTGTTTTCAAGGAAGA+TGG	+	chr8.4:67777068-67777087	MS.gene79713:CDS	40.0%
	AAGAAGGTTTGGATTGCTGC+TGG	+	chr8.4:67777194-67777213	MS.gene79713:CDS	45.0%
	AGAAGGTTTGGATTGCTGCT+GGG	+	chr8.4:67777195-67777214	MS.gene79713:CDS	45.0%
	AGATGGTCAAGAATACGCAC+AGG	+	chr8.4:67777085-67777104	MS.gene79713:CDS	45.0%
	ATGCCGAAGAACAAGGGAAA+GGG	+	chr8.4:67776996-67777015	MS.gene79713:CDS	45.0%
	CCTTCTTGTGCATCTTTCCA+CGG	-	chr8.4:67777181-67777200	None:intergenic	45.0%
	CTTGAAGTATATGCCGGATG+AGG	+	chr8.4:67777271-67777290	MS.gene79713:CDS	45.0%
	TCATTAAGCCTCACACCTTC+AGG	-	chr8.4:67777326-67777345	None:intergenic	45.0%
	TGTGAAGCCATGTGTATCGA+TGG	+	chr8.4:67777134-67777153	MS.gene79713:CDS	45.0%
	TTCAACAATCTCGCCTCATC+CGG	-	chr8.4:67777287-67777306	None:intergenic	45.0%
!	GAAGGTGTGAGGCTTAATGA+AGG	+	chr8.4:67777326-67777345	MS.gene79713:CDS	45.0%
!	GATTGATGGGGATGATGATG+AGG	+	chr8.4:67777361-67777380	MS.gene79713:CDS	45.0%
!	TGTTGGGTTGCGTGATTATC+AGG	+	chr8.4:67777229-67777248	MS.gene79713:CDS	45.0%
!!	AATTGGTGGTGGGATTGATG+GGG	+	chr8.4:67777349-67777368	MS.gene79713:CDS	45.0%
!!	GAATTGGTGGTGGGATTGAT+GGG	+	chr8.4:67777348-67777367	MS.gene79713:CDS	45.0%
	AAGCGTCTCTGTCATATCCG+TGG	+	chr8.4:67777161-67777180	MS.gene79713:CDS	50.0%
	CCGTGGAAAGATGCACAAGA+AGG	+	chr8.4:67777178-67777197	MS.gene79713:CDS	50.0%
	CCTCCCTTTCCCTTGTTCTT+CGG	-	chr8.4:67777002-67777021	None:intergenic	50.0%
	GAAGGTTTGGATTGCTGCTG+GGG	+	chr8.4:67777196-67777215	MS.gene79713:CDS	50.0%
	GCACAGGTTCTTCGTATGCT+TGG	+	chr8.4:67777101-67777120	MS.gene79713:CDS	50.0%
	GGAGGAAAGAATCGCAAGAG+AGG	+	chr8.4:67777017-67777036	MS.gene79713:CDS	50.0%
	GGATGAGGCGAGATTGTTGA+AGG	+	chr8.4:67777286-67777305	MS.gene79713:CDS	50.0%
!	GGGATGATGATGAGGGTGAT+GGG	+	chr8.4:67777369-67777388	MS.gene79713:CDS	50.0%
!!	GGAATTGGTGGTGGGATTGA+TGG	+	chr8.4:67777347-67777366	MS.gene79713:CDS	50.0%
	CCGAAGAACAAGGGAAAGGG+AGG	+	chr8.4:67776999-67777018	MS.gene79713:CDS	55.0%
	GCGTATAGCGAGCTTCCTGA+AGG	+	chr8.4:67777308-67777327	MS.gene79713:CDS	55.0%
!	GGGGATGATGATGAGGGTGA+TGG	+	chr8.4:67777368-67777387	MS.gene79713:CDS	55.0%
!	GCGAGCTTCCTGAAGGTGTG+AGG	+	chr8.4:67777315-67777334	MS.gene79713:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	67776996	67777436	67776996	ID=MS.gene79713
chr8.4	mRNA	67776996	67777436	67776996	ID=MS.gene79713.t1;Parent=MS.gene79713
chr8.4	exon	67776996	67777436	67776996	ID=MS.gene79713.t1.exon1;Parent=MS.gene79713.t1
chr8.4	CDS	67776996	67777436	67776996	ID=cds.MS.gene79713.t1;Parent=MS.gene79713.t1

Gene Sequence

>MS.gene79713

ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAGAGAGGAAAGAACGAAGCAGATGACGAAAAGCGTGAGCTTGTTTTCAAGGAAGATGGTCAAGAATACGCACAGGTTCTTCGTATGCTTGGAAACGGTCGTTGTGAAGCCATGTGTATCGATGGAACAAAGCGTCTCTGTCATATCCGTGGAAAGATGCACAAGAAGGTTTGGATTGCTGCTGGGGATATTATTCTTGTTGGGTTGCGTGATTATCAGGATGATAAGGCTGATGTGATCTTGAAGTATATGCCGGATGAGGCGAGATTGTTGAAGGCGTATAGCGAGCTTCCTGAAGGTGTGAGGCTTAATGAAGGAATTGGTGGTGGGATTGATGGGGATGATGATGAGGGTGATGGGAATGATTATATTGAGTTTGAGGATGAGGATATTGATAAGATTTGA

Protein sequence

>MS.gene79713.t1

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYSELPEGVRLNEGIGGGIDGDDDEGDGNDYIEFEDEDIDKI