AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053456


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053456.t1 MTR_1g045060 94.545 165 9 0 1 165 127 291 7.98e-109 312
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 27 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTATTGTTGTGTTGGTTT+TGG 0.222439 1.4:+29391677 MS.gene053456:CDS
GATGTTGTTTATGGTTTGTT+AGG 0.274275 1.4:+29391375 MS.gene053456:CDS
GGTGTTGCTGATGTTGTTTA+TGG 0.290325 1.4:+29391366 MS.gene053456:CDS
GTTATGGATAAAGGTGTTTC+AGG 0.302786 1.4:+29391345 MS.gene053456:CDS
GAAAGGGTGTAAGAAGATTT+CGG 0.323501 1.4:+29391551 MS.gene053456:CDS
TCTTTGTATACTGATTTGTT+TGG 0.358185 1.4:+29391525 MS.gene053456:CDS
TGATGTGAAGGGACCGTTGC+TGG 0.377985 1.4:+29391749 MS.gene053456:CDS
TTTGATGGAGTATGATGTTA+TGG 0.377988 1.4:+29391329 None:intergenic
TTTGAGAAGTGTTGCTATGA+TGG 0.447435 1.4:+29391635 MS.gene053456:CDS
TTTACATGCTATTGTTGTGT+TGG 0.447501 1.4:+29391671 MS.gene053456:CDS
GAGTATGATGTTATGGATAA+AGG 0.456867 1.4:+29391336 MS.gene053456:CDS
TCGGAGTGTTGTCGTGGGGC+AGG 0.467751 1.4:+29391712 MS.gene053456:CDS
GTTGTTTATGGTTTGTTAGG+TGG 0.472788 1.4:+29391378 MS.gene053456:CDS
ACTGATTTGTTTGGATTGAA+AGG 0.476700 1.4:+29391534 MS.gene053456:CDS
CTGATTTGTTTGGATTGAAA+GGG 0.503736 1.4:+29391535 MS.gene053456:CDS
ATTGTTCGGAGTGTTGTCGT+GGG 0.561498 1.4:+29391707 MS.gene053456:CDS
GATTGTTCGGAGTGTTGTCG+TGG 0.572455 1.4:+29391706 MS.gene053456:CDS
GATGCTTCAGAGATCGAGAT+GGG 0.595874 1.4:+29391804 MS.gene053456:CDS
TTTGAGATGTGGTGATGTGA+AGG 0.605882 1.4:+29391737 MS.gene053456:CDS
GATTCAAGATCAGCCAGCAA+CGG 0.642150 1.4:-29391762 None:intergenic
ATCACACAATCTCATACTCG+TGG 0.648924 1.4:-29391784 None:intergenic
TTGTTCGGAGTGTTGTCGTG+GGG 0.669223 1.4:+29391708 MS.gene053456:CDS
TTGAGATGTGGTGATGTGAA+GGG 0.669284 1.4:+29391738 MS.gene053456:CDS
TGATGCTTCAGAGATCGAGA+TGG 0.672172 1.4:+29391803 MS.gene053456:CDS
ACATCGACATTCTCAATCCG+CGG 0.681866 1.4:-29391588 None:intergenic
TGGGGCAGGAATTTGAGATG+TGG 0.695706 1.4:+29391726 MS.gene053456:CDS
ATGCTTCAGAGATCGAGATG+GGG 0.746310 1.4:+29391805 MS.gene053456:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTGTATACTGATTTGTT+TGG + chr1.4:29391525-29391544 MS.gene053456:CDS 25.0%
GAGTATGATGTTATGGATAA+AGG + chr1.4:29391336-29391355 MS.gene053456:CDS 30.0%
TTTACATGCTATTGTTGTGT+TGG + chr1.4:29391671-29391690 MS.gene053456:CDS 30.0%
! TGTTTTATGTGATCTTGATG+AGG + chr1.4:29391608-29391627 MS.gene053456:CDS 30.0%
!! ACTGATTTGTTTGGATTGAA+AGG + chr1.4:29391534-29391553 MS.gene053456:CDS 30.0%
!! CTGATTTGTTTGGATTGAAA+GGG + chr1.4:29391535-29391554 MS.gene053456:CDS 30.0%
!! GATGTTGTTTATGGTTTGTT+AGG + chr1.4:29391375-29391394 MS.gene053456:CDS 30.0%
!! GTACTTGGTTTTTGCAAATT+GGG + chr1.4:29391499-29391518 MS.gene053456:CDS 30.0%
! GAAAGGGTGTAAGAAGATTT+CGG + chr1.4:29391551-29391570 MS.gene053456:CDS 35.0%
! TAAACCTAATGCGTTTTTCG+CGG + chr1.4:29391443-29391462 MS.gene053456:CDS 35.0%
! TGTTGTGGGATGGTTTTTAA+GGG + chr1.4:29391477-29391496 MS.gene053456:CDS 35.0%
!! GGTACTTGGTTTTTGCAAAT+TGG + chr1.4:29391498-29391517 MS.gene053456:CDS 35.0%
!! GTTATGGATAAAGGTGTTTC+AGG + chr1.4:29391345-29391364 MS.gene053456:CDS 35.0%
!! GTTGTTTATGGTTTGTTAGG+TGG + chr1.4:29391378-29391397 MS.gene053456:CDS 35.0%
!! TTTGAGAAGTGTTGCTATGA+TGG + chr1.4:29391635-29391654 MS.gene053456:CDS 35.0%
!!! GCTATTGTTGTGTTGGTTTT+GGG + chr1.4:29391678-29391697 MS.gene053456:CDS 35.0%
!!! TGCTATTGTTGTGTTGGTTT+TGG + chr1.4:29391677-29391696 MS.gene053456:CDS 35.0%
ATCACACAATCTCATACTCG+TGG - chr1.4:29391787-29391806 None:intergenic 40.0%
! CGGAGTTTTTCTTGTGTTGT+GGG + chr1.4:29391463-29391482 MS.gene053456:CDS 40.0%
! GTGTTGTGGGATGGTTTTTA+AGG + chr1.4:29391476-29391495 MS.gene053456:CDS 40.0%
! GTTTTTCTTGTGTTGTGGGA+TGG + chr1.4:29391467-29391486 MS.gene053456:CDS 40.0%
! TTGAGATGTGGTGATGTGAA+GGG + chr1.4:29391738-29391757 MS.gene053456:CDS 40.0%
! TTTGAGATGTGGTGATGTGA+AGG + chr1.4:29391737-29391756 MS.gene053456:CDS 40.0%
!! GGTGTTGCTGATGTTGTTTA+TGG + chr1.4:29391366-29391385 MS.gene053456:CDS 40.0%
!!! GGATGGTTTTTAAGGGTACT+TGG + chr1.4:29391484-29391503 MS.gene053456:CDS 40.0%
!!! GTTTTGGGACTTGGATTGTT+CGG + chr1.4:29391693-29391712 MS.gene053456:CDS 40.0%
AACTCCGCGAAAAACGCATT+AGG - chr1.4:29391450-29391469 None:intergenic 45.0%
ACATCGACATTCTCAATCCG+CGG - chr1.4:29391591-29391610 None:intergenic 45.0%
ATGCTTCAGAGATCGAGATG+GGG + chr1.4:29391805-29391824 MS.gene053456:CDS 45.0%
GATGCTTCAGAGATCGAGAT+GGG + chr1.4:29391804-29391823 MS.gene053456:CDS 45.0%
GATTCAAGATCAGCCAGCAA+CGG - chr1.4:29391765-29391784 None:intergenic 45.0%
TGATGCTTCAGAGATCGAGA+TGG + chr1.4:29391803-29391822 MS.gene053456:CDS 45.0%
! GCGGAGTTTTTCTTGTGTTG+TGG + chr1.4:29391462-29391481 MS.gene053456:CDS 45.0%
!! ATTGTTCGGAGTGTTGTCGT+GGG + chr1.4:29391707-29391726 MS.gene053456:CDS 45.0%
!!! GTTGTGTTGGTTTTGGGACT+TGG + chr1.4:29391684-29391703 MS.gene053456:CDS 45.0%
! TGGGGCAGGAATTTGAGATG+TGG + chr1.4:29391726-29391745 MS.gene053456:CDS 50.0%
!! GATTGTTCGGAGTGTTGTCG+TGG + chr1.4:29391706-29391725 MS.gene053456:CDS 50.0%
!! TTGTTCGGAGTGTTGTCGTG+GGG + chr1.4:29391708-29391727 MS.gene053456:CDS 50.0%
!!! CGGTTTTGAGTTTGAGTCCG+CGG + chr1.4:29391571-29391590 MS.gene053456:CDS 50.0%
TGATGTGAAGGGACCGTTGC+TGG + chr1.4:29391749-29391768 MS.gene053456:CDS 55.0%
!! TCGGAGTGTTGTCGTGGGGC+AGG + chr1.4:29391712-29391731 MS.gene053456:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 29391333 29391830 29391333 ID=MS.gene053456
chr1.4 mRNA 29391333 29391830 29391333 ID=MS.gene053456.t1;Parent=MS.gene053456
chr1.4 exon 29391333 29391830 29391333 ID=MS.gene053456.t1.exon1;Parent=MS.gene053456.t1
chr1.4 CDS 29391333 29391830 29391333 ID=cds.MS.gene053456.t1;Parent=MS.gene053456.t1
Gene Sequence

>MS.gene053456

ATGGAGTATGATGTTATGGATAAAGGTGTTTCAGGTGTTGCTGATGTTGTTTATGGTTTGTTAGGTGGATTGACACTTGTGTGTGCTTTTGCATGTTTTTATTTGTCTGTTAAACCTAATGCGTTTTTCGCGGAGTTTTTCTTGTGTTGTGGGATGGTTTTTAAGGGTACTTGGTTTTTGCAAATTGGGTTTTCTTTGTATACTGATTTGTTTGGATTGAAAGGGTGTAAGAAGATTTCGGTTTTGAGTTTGAGTCCGCGGATTGAGAATGTCGATGTTTTATGTGATCTTGATGAGGATAGTTTGAGAAGTGTTGCTATGATGGAGCTTTTGTTTATTTTACATGCTATTGTTGTGTTGGTTTTGGGACTTGGATTGTTCGGAGTGTTGTCGTGGGGCAGGAATTTGAGATGTGGTGATGTGAAGGGACCGTTGCTGGCTGATCTTGAATCCACGAGTATGAGATTGTGTGATGCTTCAGAGATCGAGATGGGGTGA

Protein sequence

>MS.gene053456.t1

MEYDVMDKGVSGVADVVYGLLGGLTLVCAFACFYLSVKPNAFFAEFFLCCGMVFKGTWFLQIGFSLYTDLFGLKGCKKISVLSLSPRIENVDVLCDLDEDSLRSVAMMELLFILHAIVVLVLGLGLFGVLSWGRNLRCGDVKGPLLADLESTSMRLCDASEIEMG