Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80609.t1 | RHN63834.1 | 85.6 | 118 | 9 | 2 | 1 | 117 | 56 | 166 | 4.70E-38 | 167.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80609.t1 | Q29PU4 | 46.5 | 71 | 18 | 2 | 51 | 117 | 64 | 118 | 9.6e-08 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80609.t1 | A0A396IHQ0 | 85.6 | 118 | 9 | 2 | 1 | 117 | 56 | 166 | 3.4e-38 | 167.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene80609 | 0.83019 | 3.38E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene80609 | 0.828329 | 9.55E-55 | -1.69E-46 |
| MS.gene051946 | MS.gene80609 | 0.82116 | 4.68E-53 | -1.69E-46 |
| MS.gene052164 | MS.gene80609 | -0.83238 | 9.77E-56 | -1.69E-46 |
| MS.gene05234 | MS.gene80609 | 0.803604 | 3.22E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene80609 | -0.803029 | 4.24E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene80609 | 0.817793 | 2.74E-52 | -1.69E-46 |
| MS.gene054003 | MS.gene80609 | -0.803001 | 4.29E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene80609 | -0.810749 | 9.87E-51 | -1.69E-46 |
| MS.gene056076 | MS.gene80609 | 0.812705 | 3.71E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene80609 | 0.818489 | 1.91E-52 | -1.69E-46 |
| MS.gene056313 | MS.gene80609 | 0.81835 | 2.05E-52 | -1.69E-46 |
| MS.gene056408 | MS.gene80609 | 0.809876 | 1.52E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene80609 | 0.807664 | 4.53E-50 | -1.69E-46 |
| MS.gene057770 | MS.gene80609 | 0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene80609 | -0.820402 | 6.99E-53 | -1.69E-46 |
| MS.gene058171 | MS.gene80609 | 0.810184 | 1.31E-50 | -1.69E-46 |
| MS.gene058173 | MS.gene80609 | 0.800426 | 1.45E-48 | -1.69E-46 |
| MS.gene058175 | MS.gene80609 | 0.808702 | 2.72E-50 | -1.69E-46 |
| MS.gene059223 | MS.gene80609 | 0.821271 | 4.41E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene80609 | -0.816667 | 4.91E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80609.t1 | MTR_8g076990 | 70.000 | 80 | 21 | 2 | 40 | 117 | 2 | 80 | 4.26e-28 | 98.2 |
| MS.gene80609.t1 | MTR_1g093800 | 46.970 | 66 | 22 | 2 | 52 | 117 | 52 | 104 | 3.35e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80609.t1 | AT1G73965 | 38.136 | 118 | 58 | 4 | 2 | 117 | 3 | 107 | 7.22e-17 | 70.5 |
| MS.gene80609.t1 | AT1G68795 | 47.761 | 67 | 23 | 1 | 51 | 117 | 64 | 118 | 3.19e-13 | 61.6 |
Find 33 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACTAGCAACACCAAGTTTC+AGG | 0.222084 | 8.4:+16435967 | MS.gene80609:CDS |
| CATTAATGGTGCAATGGATT+TGG | 0.262090 | 8.4:-16436181 | None:intergenic |
| AGTTTCAGGTTTATCAAAAT+AGG | 0.287858 | 8.4:+16435981 | MS.gene80609:CDS |
| ACTTCACTCCATTTCTTAAT+CGG | 0.308385 | 8.4:+16436026 | MS.gene80609:CDS |
| TAATAAAACTAAGTACATAT+TGG | 0.410828 | 8.4:-16435862 | None:intergenic |
| ACTGAGATCGATCCGCGTTA+TGG | 0.417933 | 8.4:+16436130 | MS.gene80609:CDS |
| GATACTCTTTCATATGTGCT+TGG | 0.418515 | 8.4:+16435904 | MS.gene80609:CDS |
| AATAGGAAAATCTTAGCAAC+CGG | 0.463874 | 8.4:+16435998 | MS.gene80609:CDS |
| GATAAACGCCTTGTTCCATC+TGG | 0.473410 | 8.4:+16436157 | MS.gene80609:CDS |
| AAATCCAATATTAGTACTAG+TGG | 0.494292 | 8.4:+16435932 | MS.gene80609:CDS |
| TCAGTTTCCTTCGGCTCATG+AGG | 0.494458 | 8.4:-16436112 | None:intergenic |
| TGGTGGTGCCGATTAAGAAA+TGG | 0.504177 | 8.4:-16436034 | None:intergenic |
| AAATGGAGTGAAGTCAAATC+CGG | 0.507048 | 8.4:-16436017 | None:intergenic |
| CCGGCATGTGACTCCGGTGG+TGG | 0.514190 | 8.4:-16436081 | None:intergenic |
| GAGTCACCGGCATGTGACTC+CGG | 0.517907 | 8.4:-16436087 | None:intergenic |
| GGCTCATGAGGGTGAGTCAC+CGG | 0.520168 | 8.4:-16436100 | None:intergenic |
| ACCACGGTCATCGTCATCAT+CGG | 0.529578 | 8.4:+16436053 | MS.gene80609:CDS |
| GGATCGATCTCAGTTTCCTT+CGG | 0.540255 | 8.4:-16436121 | None:intergenic |
| CCACGGTCATCGTCATCATC+GGG | 0.543735 | 8.4:+16436054 | MS.gene80609:CDS |
| TGCAATGGATTTGGACCAGA+TGG | 0.557410 | 8.4:-16436172 | None:intergenic |
| GAACCTCATTAATGGTGCAA+TGG | 0.574590 | 8.4:-16436187 | None:intergenic |
| CCACCACCGGAGTCACATGC+CGG | 0.579118 | 8.4:+16436081 | MS.gene80609:CDS |
| AAACTTGGTGTTGCTAGTAA+TGG | 0.582271 | 8.4:-16435963 | None:intergenic |
| ATATTGGAGTTTGATAGTCA+TGG | 0.587744 | 8.4:-16435846 | None:intergenic |
| CAGTTTCCTTCGGCTCATGA+GGG | 0.590230 | 8.4:-16436111 | None:intergenic |
| GACTCACCCTCATGAGCCGA+AGG | 0.604155 | 8.4:+16436105 | MS.gene80609:CDS |
| GATTTGGACCAGATGGAACA+AGG | 0.607957 | 8.4:-16436165 | None:intergenic |
| ATCATCGGGATCACCACCAC+CGG | 0.629549 | 8.4:+16436068 | MS.gene80609:CDS |
| TCACCGGCATGTGACTCCGG+TGG | 0.690195 | 8.4:-16436084 | None:intergenic |
| TTTCTTAATCGGCACCACCA+CGG | 0.698743 | 8.4:+16436037 | MS.gene80609:CDS |
| CGTTTATCAACACCATAACG+CGG | 0.733230 | 8.4:-16436142 | None:intergenic |
| CCCGATGATGACGATGACCG+TGG | 0.746981 | 8.4:-16436054 | None:intergenic |
| GATGATGACGATGACCGTGG+TGG | 0.778203 | 8.4:-16436051 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAAAACTAAGTACATAT+TGG | - | chr8.4:16435865-16435884 | None:intergenic | 15.0% |
| !!! | ATGTACTTAGTTTTATTATT+TGG | + | chr8.4:16435867-16435886 | MS.gene80609:CDS | 15.0% |
| ! | AAATCCAATATTAGTACTAG+TGG | + | chr8.4:16435932-16435951 | MS.gene80609:CDS | 25.0% |
| !! | TTTTCCACTAGTACTAATAT+TGG | - | chr8.4:16435939-16435958 | None:intergenic | 25.0% |
| !!! | AGTTTCAGGTTTATCAAAAT+AGG | + | chr8.4:16435981-16436000 | MS.gene80609:CDS | 25.0% |
| !!! | ATTTTGATAAACCTGAAACT+TGG | - | chr8.4:16435981-16436000 | None:intergenic | 25.0% |
| AATAGGAAAATCTTAGCAAC+CGG | + | chr8.4:16435998-16436017 | MS.gene80609:CDS | 30.0% | |
| ACTTCACTCCATTTCTTAAT+CGG | + | chr8.4:16436026-16436045 | MS.gene80609:CDS | 30.0% | |
| AAATGGAGTGAAGTCAAATC+CGG | - | chr8.4:16436020-16436039 | None:intergenic | 35.0% | |
| GATACTCTTTCATATGTGCT+TGG | + | chr8.4:16435904-16435923 | MS.gene80609:CDS | 35.0% | |
| !! | AAACTTGGTGTTGCTAGTAA+TGG | - | chr8.4:16435966-16435985 | None:intergenic | 35.0% |
| CGTTTATCAACACCATAACG+CGG | - | chr8.4:16436145-16436164 | None:intergenic | 40.0% | |
| TACTAGCAACACCAAGTTTC+AGG | + | chr8.4:16435967-16435986 | MS.gene80609:CDS | 40.0% | |
| GATTTGGACCAGATGGAACA+AGG | - | chr8.4:16436168-16436187 | None:intergenic | 45.0% | |
| GGATCGATCTCAGTTTCCTT+CGG | - | chr8.4:16436124-16436143 | None:intergenic | 45.0% | |
| TTTCTTAATCGGCACCACCA+CGG | + | chr8.4:16436037-16436056 | MS.gene80609:CDS | 45.0% | |
| ! | GATAAACGCCTTGTTCCATC+TGG | + | chr8.4:16436157-16436176 | MS.gene80609:CDS | 45.0% |
| ! | TGCAATGGATTTGGACCAGA+TGG | - | chr8.4:16436175-16436194 | None:intergenic | 45.0% |
| !! | TGGTGGTGCCGATTAAGAAA+TGG | - | chr8.4:16436037-16436056 | None:intergenic | 45.0% |
| ACCACGGTCATCGTCATCAT+CGG | + | chr8.4:16436053-16436072 | MS.gene80609:CDS | 50.0% | |
| ACTGAGATCGATCCGCGTTA+TGG | + | chr8.4:16436130-16436149 | MS.gene80609:CDS | 50.0% | |
| CAGTTTCCTTCGGCTCATGA+GGG | - | chr8.4:16436114-16436133 | None:intergenic | 50.0% | |
| TCAGTTTCCTTCGGCTCATG+AGG | - | chr8.4:16436115-16436134 | None:intergenic | 50.0% | |
| ATCATCGGGATCACCACCAC+CGG | + | chr8.4:16436068-16436087 | MS.gene80609:CDS | 55.0% | |
| CCACGGTCATCGTCATCATC+GGG | + | chr8.4:16436054-16436073 | MS.gene80609:CDS | 55.0% | |
| ! | GATGATGACGATGACCGTGG+TGG | - | chr8.4:16436054-16436073 | None:intergenic | 55.0% |
| GACTCACCCTCATGAGCCGA+AGG | + | chr8.4:16436105-16436124 | MS.gene80609:CDS | 60.0% | |
| GAGTCACCGGCATGTGACTC+CGG | - | chr8.4:16436090-16436109 | None:intergenic | 60.0% | |
| GGCTCATGAGGGTGAGTCAC+CGG | - | chr8.4:16436103-16436122 | None:intergenic | 60.0% | |
| ! | CCCGATGATGACGATGACCG+TGG | - | chr8.4:16436057-16436076 | None:intergenic | 60.0% |
| CCACCACCGGAGTCACATGC+CGG | + | chr8.4:16436081-16436100 | MS.gene80609:CDS | 65.0% | |
| TCACCGGCATGTGACTCCGG+TGG | - | chr8.4:16436087-16436106 | None:intergenic | 65.0% | |
| CCGGCATGTGACTCCGGTGG+TGG | - | chr8.4:16436084-16436103 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 16435848 | 16436201 | 16435848 | ID=MS.gene80609 |
| chr8.4 | mRNA | 16435848 | 16436201 | 16435848 | ID=MS.gene80609.t1;Parent=MS.gene80609 |
| chr8.4 | exon | 16435848 | 16436201 | 16435848 | ID=MS.gene80609.t1.exon1;Parent=MS.gene80609.t1 |
| chr8.4 | CDS | 16435848 | 16436201 | 16435848 | ID=cds.MS.gene80609.t1;Parent=MS.gene80609.t1 |
| Gene Sequence |
| Protein sequence |
