Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82687.t1 | XP_024634879.1 | 83.3 | 580 | 85 | 4 | 1 | 576 | 209 | 780 | 2.60E-246 | 861.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82687.t1 | A0A396IRE0 | 84.9 | 345 | 52 | 0 | 232 | 576 | 1 | 345 | 2.5e-158 | 568.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049435 | MS.gene82687 | 0.812155 | 4.89E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene82687 | 0.812127 | 4.96E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene82687 | 0.822594 | 2.18E-53 | -1.69E-46 |
| MS.gene052665 | MS.gene82687 | 0.801342 | 9.42E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene82687 | 0.819473 | 1.14E-52 | -1.69E-46 |
| MS.gene053166 | MS.gene82687 | 0.80504 | 1.62E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene82687 | 0.806288 | 8.85E-50 | -1.69E-46 |
| MS.gene055356 | MS.gene82687 | 0.802959 | 4.38E-49 | -1.69E-46 |
| MS.gene055357 | MS.gene82687 | 0.800928 | 1.14E-48 | -1.69E-46 |
| MS.gene055358 | MS.gene82687 | 0.800599 | 1.34E-48 | -1.69E-46 |
| MS.gene055359 | MS.gene82687 | 0.803876 | 2.83E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene82687 | 0.804485 | 2.11E-49 | -1.69E-46 |
| MS.gene055926 | MS.gene82687 | 0.816985 | 4.17E-52 | -1.69E-46 |
| MS.gene056173 | MS.gene82687 | 0.805023 | 1.63E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene82687 | 0.822617 | 2.15E-53 | -1.69E-46 |
| MS.gene059006 | MS.gene82687 | 0.813197 | 2.89E-51 | -1.69E-46 |
| MS.gene060137 | MS.gene82687 | -0.818987 | 1.47E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene82687 | 0.822517 | 2.27E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTGCCTCTCACATTCTA+TGG | 0.260173 | 3.1:-37720637 | MS.gene82687:CDS |
| AACCCCAGGTGAAATCTTTA+AGG | 0.271604 | 3.1:-37721085 | MS.gene82687:CDS |
| ATGTTCATTTCTTTCATTGC+TGG | 0.288747 | 3.1:-37720727 | MS.gene82687:CDS |
| AAGTGGCGTAGTTGAAATTA+TGG | 0.312736 | 3.1:-37721939 | MS.gene82687:CDS |
| AATTCATCAACATTGTATAA+TGG | 0.327435 | 3.1:+37724068 | None:intergenic |
| CTTCTTTGTTGCTTCTATGA+TGG | 0.336687 | 3.1:+37721112 | None:intergenic |
| CCTGTTTCCTGATTGCCTCC+AGG | 0.337339 | 3.1:+37720946 | None:intergenic |
| GATCAAGATATAACGGAAAC+TGG | 0.338760 | 3.1:+37720608 | None:intergenic |
| TTCAAGTGCACGCATGATTT+CGG | 0.351916 | 3.1:+37721787 | None:intergenic |
| CATTGTCAGAGTTAGTTTCA+TGG | 0.358694 | 3.1:+37721744 | None:intergenic |
| TTTGCTGTATCTTCATTAGT+TGG | 0.362700 | 3.1:-37720886 | MS.gene82687:CDS |
| CCTGGAGGCAATCAGGAAAC+AGG | 0.362749 | 3.1:-37720946 | MS.gene82687:CDS |
| TCCTTTCTATACTCACTTCT+CGG | 0.368888 | 3.1:-37720831 | MS.gene82687:CDS |
| AAATGTTTGTATTAACCATA+TGG | 0.371774 | 3.1:-37723971 | MS.gene82687:CDS |
| AAAACTGCTTGGAGGATATC+TGG | 0.377606 | 3.1:-37721560 | MS.gene82687:CDS |
| CGCAAGTTGTTGAGGGGTTG+TGG | 0.380881 | 3.1:-37721685 | MS.gene82687:CDS |
| AAGAAAGAAATCAAAACAAA+TGG | 0.391933 | 3.1:-37722059 | MS.gene82687:CDS |
| TGTTGCTTCTATGATGGTAA+TGG | 0.392154 | 3.1:+37721118 | None:intergenic |
| AGCTTAACACCCTTGATACT+TGG | 0.393569 | 3.1:+37720553 | None:intergenic |
| TTTATTACTTGTAGCAATGA+AGG | 0.395604 | 3.1:-37721876 | MS.gene82687:CDS |
| CTGTTTCCTGATTGCCTCCA+GGG | 0.400780 | 3.1:+37720947 | None:intergenic |
| TCCTCCTTAAAGATTTCACC+TGG | 0.410908 | 3.1:+37721081 | None:intergenic |
| GTTGTGGAAGAGGCTTTCTA+TGG | 0.418288 | 3.1:-37721669 | MS.gene82687:CDS |
| CAACGTCCCTGGAGGCAATC+AGG | 0.419195 | 3.1:-37720953 | MS.gene82687:CDS |
| CTCTTGAAATTCTTGCTACT+AGG | 0.427955 | 3.1:-37739388 | MS.gene82687:CDS |
| GGCGTCTCCTTTGCTACATC+AGG | 0.434602 | 3.1:-37720976 | MS.gene82687:CDS |
| TTGTTGAGGGGTTGTGGAAG+AGG | 0.439045 | 3.1:-37721679 | MS.gene82687:CDS |
| CATATTCGGTAGCCAGCTCT+TGG | 0.441519 | 3.1:-37723416 | MS.gene82687:CDS |
| CTTCTATGATGGTAATGGTC+TGG | 0.452731 | 3.1:+37721123 | None:intergenic |
| GGGGCTCATCCCTTGGATTA+CGG | 0.453441 | 3.1:-37723352 | MS.gene82687:CDS |
| TCATAAATGCTTCCAAGAGC+TGG | 0.458200 | 3.1:+37723404 | None:intergenic |
| GTTAAGAAGAAGCACATATT+CGG | 0.460043 | 3.1:-37723430 | MS.gene82687:CDS |
| ACAGGGAAACCAACATTTGA+AGG | 0.475667 | 3.1:-37720928 | MS.gene82687:CDS |
| AACCTTAGGGGCAATGAAGA+TGG | 0.479996 | 3.1:-37723452 | MS.gene82687:intron |
| GAAGGGCAACCAGCATTTGA+AGG | 0.483339 | 3.1:-37720910 | MS.gene82687:CDS |
| TGAAAATAGGGTATGCCATA+TGG | 0.485165 | 3.1:+37723956 | None:intergenic |
| ATAGAACAAGATACCACTCT+TGG | 0.493665 | 3.1:+37720675 | None:intergenic |
| CTCCTTAAAGATTTCACCTG+GGG | 0.497170 | 3.1:+37721083 | None:intergenic |
| GCTACATCAGGCAACGTCCC+TGG | 0.497506 | 3.1:-37720964 | MS.gene82687:CDS |
| AGAGCTTAATTCAAAAGTAA+AGG | 0.498132 | 3.1:-37721915 | MS.gene82687:CDS |
| GTGTGGTGGGGCTCATCCCT+TGG | 0.500121 | 3.1:-37723359 | MS.gene82687:CDS |
| GAATGTGAGAGGCAAGCAAA+TGG | 0.502669 | 3.1:+37720642 | None:intergenic |
| GTTGTAGCAGCACTCGTCGC+AGG | 0.505518 | 3.1:-37720997 | MS.gene82687:CDS |
| AGAAAGAAATCAAAACAAAT+GGG | 0.508367 | 3.1:-37722058 | MS.gene82687:CDS |
| GGTGCCACCACCAAGTATCA+AGG | 0.511463 | 3.1:-37720563 | MS.gene82687:CDS |
| TTGAGAAGATCAAGATATAA+CGG | 0.512499 | 3.1:+37720601 | None:intergenic |
| CCACACATGTATGACTCATA+AGG | 0.512671 | 3.1:+37723376 | None:intergenic |
| TTGATCTTCTCAAAGTTACA+TGG | 0.512948 | 3.1:-37720591 | MS.gene82687:CDS |
| AGAAATGAACATGGCAACAA+TGG | 0.515982 | 3.1:+37720738 | None:intergenic |
| ACAGCAAATCCTTCAAATGC+TGG | 0.519658 | 3.1:+37720901 | None:intergenic |
| TTAACACCCTTGATACTTGG+TGG | 0.523933 | 3.1:+37720556 | None:intergenic |
| GCTGGTTGCCCTTCAAATGT+TGG | 0.529783 | 3.1:+37720919 | None:intergenic |
| ATGAGTCATACATGTGTGGT+GGG | 0.531314 | 3.1:-37723372 | MS.gene82687:CDS |
| CACAACCCCTCAACAACTTG+CGG | 0.534637 | 3.1:+37721686 | None:intergenic |
| TTGAAAGTGTCATGCACGAA+AGG | 0.536960 | 3.1:-37723291 | MS.gene82687:CDS |
| TTGTTGATTTATGCTCTGAA+AGG | 0.537416 | 3.1:+37721643 | None:intergenic |
| GTCATGTTCTCCGTAATCCA+AGG | 0.537829 | 3.1:+37723342 | None:intergenic |
| AGACAACCGCAAGTTGTTGA+GGG | 0.537910 | 3.1:-37721692 | MS.gene82687:CDS |
| GTAAAGGCATCTGACAAGAA+AGG | 0.540805 | 3.1:-37721899 | MS.gene82687:CDS |
| CATCATAGAAGCAACAAAGA+AGG | 0.547820 | 3.1:-37721111 | MS.gene82687:CDS |
| TCCGAGAAGTGAGTATAGAA+AGG | 0.552792 | 3.1:+37720830 | None:intergenic |
| TAGACAACCGCAAGTTGTTG+AGG | 0.558579 | 3.1:-37721693 | MS.gene82687:CDS |
| GATGTCCCACATCATTTGCA+TGG | 0.565189 | 3.1:+37721532 | None:intergenic |
| CGCTCTACAGAGGACAAACA+TGG | 0.568320 | 3.1:-37739419 | None:intergenic |
| CCTCCTTAAAGATTTCACCT+GGG | 0.568420 | 3.1:+37721082 | None:intergenic |
| TATGAGTCATACATGTGTGG+TGG | 0.583051 | 3.1:-37723373 | MS.gene82687:CDS |
| AAATCAAAACAAATGGGAGA+AGG | 0.587759 | 3.1:-37722052 | MS.gene82687:CDS |
| TATTTGCGTGCAGCAAAACA+TGG | 0.589012 | 3.1:-37721815 | MS.gene82687:CDS |
| GACGTTGCCTGATGTAGCAA+AGG | 0.589787 | 3.1:+37720969 | None:intergenic |
| ATGGTCTACTCAGTCACCGT+GGG | 0.591157 | 3.1:-37723919 | MS.gene82687:CDS |
| ACACCCTTGATACTTGGTGG+TGG | 0.591476 | 3.1:+37720559 | None:intergenic |
| GTGGGACATCAAGTGGTACA+AGG | 0.594742 | 3.1:-37721519 | MS.gene82687:intron |
| TCAAGGGTGTTAAGCTGTAG+AGG | 0.595960 | 3.1:-37720546 | None:intergenic |
| ACAGATAAATATTCCAAGAG+TGG | 0.603652 | 3.1:-37720688 | MS.gene82687:CDS |
| CTGGAGGCAATCAGGAAACA+GGG | 0.606342 | 3.1:-37720945 | MS.gene82687:CDS |
| CTAGTAGCAAGAATTTCAAG+AGG | 0.608826 | 3.1:+37739389 | None:intergenic |
| GAATTACCAGCAAAGAAAAG+TGG | 0.610189 | 3.1:-37721854 | MS.gene82687:CDS |
| CAGGGAAACCAACATTTGAA+GGG | 0.612015 | 3.1:-37720927 | MS.gene82687:CDS |
| AGTGTCATGCACGAAAGGAA+AGG | 0.614504 | 3.1:-37723286 | MS.gene82687:intron |
| GTGCGGCCATGCAAATGATG+TGG | 0.618816 | 3.1:-37721538 | MS.gene82687:CDS |
| TCTCAAAGTTACATGGAGGA+AGG | 0.625653 | 3.1:-37720584 | MS.gene82687:CDS |
| GTGCCACCACCAAGTATCAA+GGG | 0.630567 | 3.1:-37720562 | MS.gene82687:CDS |
| TCATGTTCTCCGTAATCCAA+GGG | 0.632076 | 3.1:+37723343 | None:intergenic |
| TTCTAGATAAAAGCATTGAG+TGG | 0.638047 | 3.1:-37721047 | MS.gene82687:CDS |
| GACAACCGCAAGTTGTTGAG+GGG | 0.642150 | 3.1:-37721691 | MS.gene82687:CDS |
| TGAGTCATACATGTGTGGTG+GGG | 0.648242 | 3.1:-37723371 | MS.gene82687:CDS |
| CCCAGGTGAAATCTTTAAGG+AGG | 0.655968 | 3.1:-37721082 | MS.gene82687:CDS |
| TGCGGCCATGCAAATGATGT+GGG | 0.663238 | 3.1:-37721537 | MS.gene82687:CDS |
| GATGGTCTACTCAGTCACCG+TGG | 0.671545 | 3.1:-37723920 | MS.gene82687:CDS |
| ACAACTCCATAGAATGTGAG+AGG | 0.673388 | 3.1:+37720631 | None:intergenic |
| GTTGAAGACATTGCAGACCA+AGG | 0.679908 | 3.1:-37734512 | MS.gene82687:intron |
| ATCTTCTCAAAGTTACATGG+AGG | 0.681695 | 3.1:-37720588 | MS.gene82687:CDS |
| AAATGATGTGGGACATCAAG+TGG | 0.684085 | 3.1:-37721526 | MS.gene82687:CDS |
| TGCTTGGAGGATATCTGGTG+CGG | 0.697268 | 3.1:-37721555 | MS.gene82687:CDS |
| AGCAATGAAAGAAATGAACA+TGG | 0.705741 | 3.1:+37720729 | None:intergenic |
| ACATCAGGCAACGTCCCTGG+AGG | 0.708851 | 3.1:-37720961 | MS.gene82687:CDS |
| CCTTATGAGTCATACATGTG+TGG | 0.718357 | 3.1:-37723376 | MS.gene82687:CDS |
| TGGTCTACTCAGTCACCGTG+GGG | 0.726499 | 3.1:-37723918 | MS.gene82687:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 37720549 | 37739431 | 37720549 | ID=MS.gene82687 |
| chr3.1 | mRNA | 37720549 | 37739431 | 37720549 | ID=MS.gene82687.t1;Parent=MS.gene82687 |
| chr3.1 | exon | 37739327 | 37739431 | 37739327 | ID=MS.gene82687.t1.exon1;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37739327 | 37739431 | 37739327 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| chr3.1 | exon | 37734513 | 37734584 | 37734513 | ID=MS.gene82687.t1.exon2;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37734513 | 37734584 | 37734513 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| chr3.1 | exon | 37723919 | 37724110 | 37723919 | ID=MS.gene82687.t1.exon3;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37723919 | 37724110 | 37723919 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| chr3.1 | exon | 37723287 | 37723466 | 37723287 | ID=MS.gene82687.t1.exon4;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37723287 | 37723466 | 37723287 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| chr3.1 | exon | 37721520 | 37722088 | 37721520 | ID=MS.gene82687.t1.exon5;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37721520 | 37722088 | 37721520 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| chr3.1 | exon | 37720549 | 37721163 | 37720549 | ID=MS.gene82687.t1.exon6;Parent=MS.gene82687.t1 |
| chr3.1 | CDS | 37720549 | 37721163 | 37720549 | ID=cds.MS.gene82687.t1;Parent=MS.gene82687.t1 |
| Gene Sequence |
| Protein sequence |
