Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84222.t1 | XP_003623278.1 | 86.8 | 182 | 21 | 2 | 5 | 183 | 39 | 220 | 7.20E-78 | 300.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84222.t1 | G7KRF4 | 86.8 | 182 | 21 | 2 | 5 | 183 | 39 | 220 | 5.2e-78 | 300.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene84222 | 0.808922 | 2.44E-50 | -1.69E-46 |
| MS.gene050096 | MS.gene84222 | 0.801444 | 8.98E-49 | -1.69E-46 |
| MS.gene050720 | MS.gene84222 | 0.820251 | 7.57E-53 | -1.69E-46 |
| MS.gene051158 | MS.gene84222 | 0.808223 | 3.44E-50 | -1.69E-46 |
| MS.gene051996 | MS.gene84222 | 0.808628 | 2.82E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene84222 | 0.806625 | 7.51E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene84222 | 0.812919 | 3.33E-51 | -1.69E-46 |
| MS.gene053319 | MS.gene84222 | 0.809061 | 2.28E-50 | -1.69E-46 |
| MS.gene053406 | MS.gene84222 | 0.849963 | 2.31E-60 | -1.69E-46 |
| MS.gene054147 | MS.gene84222 | 0.81676 | 4.68E-52 | -1.69E-46 |
| MS.gene054277 | MS.gene84222 | 0.807223 | 5.61E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene84222 | 0.838351 | 3.03E-57 | -1.69E-46 |
| MS.gene055240 | MS.gene84222 | 0.811738 | 6.02E-51 | -1.69E-46 |
| MS.gene055601 | MS.gene84222 | 0.803912 | 2.78E-49 | -1.69E-46 |
| MS.gene056076 | MS.gene84222 | 0.806385 | 8.44E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene84222 | 0.841517 | 4.53E-58 | -1.69E-46 |
| MS.gene056680 | MS.gene84222 | 0.834281 | 3.28E-56 | -1.69E-46 |
| MS.gene056920 | MS.gene84222 | 0.84428 | 8.34E-59 | -1.69E-46 |
| MS.gene056922 | MS.gene84222 | 0.804386 | 2.21E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene84222 | 0.83147 | 1.64E-55 | -1.69E-46 |
| MS.gene059891 | MS.gene84222 | 0.812389 | 4.35E-51 | -1.69E-46 |
| MS.gene059892 | MS.gene84222 | 0.818807 | 1.62E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene84222 | 0.828386 | 9.25E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84222.t1 | MTR_7g068110 | 86.813 | 182 | 21 | 2 | 5 | 183 | 39 | 220 | 7.18e-109 | 310 |
| MS.gene84222.t1 | MTR_7g068140 | 54.497 | 189 | 80 | 3 | 1 | 183 | 35 | 223 | 1.49e-57 | 180 |
| MS.gene84222.t1 | MTR_3g062340 | 28.105 | 153 | 110 | 0 | 29 | 181 | 1795 | 1947 | 6.32e-14 | 69.7 |
| MS.gene84222.t1 | MTR_3g062420 | 25.153 | 163 | 108 | 1 | 18 | 180 | 1790 | 1938 | 2.63e-12 | 64.7 |
| MS.gene84222.t1 | MTR_3g466750 | 24.183 | 153 | 116 | 0 | 29 | 181 | 1714 | 1866 | 3.29e-12 | 64.7 |
| MS.gene84222.t1 | MTR_2g049960 | 23.529 | 153 | 117 | 0 | 29 | 181 | 169 | 321 | 1.82e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCGACTCTAAATATTAAA+AGG | 0.195339 | 7.1:+33705348 | None:intergenic |
| TAATAGGAAGCAGCAAATTA+TGG | 0.255664 | 7.1:-33705394 | MS.gene84222:CDS |
| CTATGGGTTACGTTTCTAAT+AGG | 0.324528 | 7.1:-33705410 | MS.gene84222:CDS |
| GCTACTAATTTGAAACTTGT+TGG | 0.326817 | 7.1:-33705510 | MS.gene84222:CDS |
| TAATGTAGTCATCAAGTCTA+AGG | 0.337618 | 7.1:+33705674 | None:intergenic |
| CTCGATGTTATTAATATGTA+TGG | 0.342114 | 7.1:-33705630 | MS.gene84222:CDS |
| ACCCATAGCATCTTTCTCAT+TGG | 0.387905 | 7.1:+33705425 | None:intergenic |
| TGCGACTCTAAATATTAAAA+GGG | 0.393156 | 7.1:+33705349 | None:intergenic |
| CTAAGGATGCGCGACTCCTC+AGG | 0.399539 | 7.1:-33705815 | MS.gene84222:CDS |
| CTGAGGAGTCGCGCATCCTT+AGG | 0.402078 | 7.1:+33705816 | None:intergenic |
| TTTGAAACTTGTTGGAGAGT+TGG | 0.425030 | 7.1:-33705502 | MS.gene84222:CDS |
| CGCATCCTTAGGTGGAAAAT+CGG | 0.437870 | 7.1:+33705827 | None:intergenic |
| TTCCAATGAGAAAGATGCTA+TGG | 0.475705 | 7.1:-33705427 | MS.gene84222:CDS |
| GAAACTTGTTGGAGAGTTGG+AGG | 0.476211 | 7.1:-33705499 | MS.gene84222:CDS |
| AGGTGAATGGCTTGATGACT+TGG | 0.498559 | 7.1:-33705601 | MS.gene84222:CDS |
| ACTTGTTGGAGAGTTGGAGG+AGG | 0.522944 | 7.1:-33705496 | MS.gene84222:CDS |
| ATTAATATGTATGGCTTCAG+AGG | 0.525794 | 7.1:-33705621 | MS.gene84222:CDS |
| TCGCAAGAGCTTGAGGAAGT+TGG | 0.534058 | 7.1:+33705707 | None:intergenic |
| TCTTTACAGGTCCTGCACCT+TGG | 0.541164 | 7.1:-33705878 | MS.gene84222:intron |
| AAAACATTACGCCAAGGTGC+AGG | 0.544422 | 7.1:+33705867 | None:intergenic |
| AGCTCCACGTCTTATGTCAA+GGG | 0.547099 | 7.1:+33705743 | None:intergenic |
| AAGCTCCACGTCTTATGTCA+AGG | 0.560627 | 7.1:+33705742 | None:intergenic |
| TAAGGATGCGCGACTCCTCA+GGG | 0.564280 | 7.1:-33705814 | MS.gene84222:CDS |
| TTCTTTGGCGATATCAAGAG+TGG | 0.567082 | 7.1:+33705560 | None:intergenic |
| TTAAAATCACATGTTGCTGA+AGG | 0.586212 | 7.1:-33705654 | MS.gene84222:CDS |
| AGGAGTCGCGCATCCTTAGG+TGG | 0.590816 | 7.1:+33705819 | None:intergenic |
| TCCAATGAGAAAGATGCTAT+GGG | 0.598675 | 7.1:-33705426 | MS.gene84222:CDS |
| TCTTGATATCGCCAAAGAAG+AGG | 0.598911 | 7.1:-33705556 | MS.gene84222:CDS |
| TTGGCGATATCAAGAGTGGA+GGG | 0.601865 | 7.1:+33705564 | None:intergenic |
| AGTGTGATCGCAAGAGCTTG+AGG | 0.610597 | 7.1:+33705700 | None:intergenic |
| TTTGGCGATATCAAGAGTGG+AGG | 0.621011 | 7.1:+33705563 | None:intergenic |
| TGTATGGCTTCAGAGGTGAA+TGG | 0.635609 | 7.1:-33705614 | MS.gene84222:CDS |
| CATCCCCTTGACATAAGACG+TGG | 0.651438 | 7.1:-33705747 | MS.gene84222:CDS |
| CGTGGTAAAACATTACGCCA+AGG | 0.657808 | 7.1:+33705861 | None:intergenic |
| GCGACTCTAAATATTAAAAG+GGG | 0.658106 | 7.1:+33705350 | None:intergenic |
| AAATCGGCAACAACAGAGCG+TGG | 0.663909 | 7.1:+33705843 | None:intergenic |
| GCTCCACGTCTTATGTCAAG+GGG | 0.665557 | 7.1:+33705744 | None:intergenic |
| GACGAGCAGAAAATTCCCTG+AGG | 0.767412 | 7.1:+33705799 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTCGATGTTATTAATATGTA+TGG | - | chr7.1:33705726-33705745 | MS.gene84222:CDS | 25.0% |
| ATTAATATGTATGGCTTCAG+AGG | - | chr7.1:33705735-33705754 | MS.gene84222:CDS | 30.0% | |
| TAATAGGAAGCAGCAAATTA+TGG | - | chr7.1:33705962-33705981 | MS.gene84222:intron | 30.0% | |
| TAATGTAGTCATCAAGTCTA+AGG | + | chr7.1:33705685-33705704 | None:intergenic | 30.0% | |
| TTAAAATCACATGTTGCTGA+AGG | - | chr7.1:33705702-33705721 | MS.gene84222:CDS | 30.0% | |
| ! | GCTACTAATTTGAAACTTGT+TGG | - | chr7.1:33705846-33705865 | MS.gene84222:CDS | 30.0% |
| !! | AGTTGATGTGTTTTCTTTAC+AGG | - | chr7.1:33705465-33705484 | MS.gene84222:CDS | 30.0% |
| TCCAATGAGAAAGATGCTAT+GGG | - | chr7.1:33705930-33705949 | MS.gene84222:intron | 35.0% | |
| TTCCAATGAGAAAGATGCTA+TGG | - | chr7.1:33705929-33705948 | MS.gene84222:intron | 35.0% | |
| ! | CTATGGGTTACGTTTCTAAT+AGG | - | chr7.1:33705946-33705965 | MS.gene84222:intron | 35.0% |
| !! | TTTGAAACTTGTTGGAGAGT+TGG | - | chr7.1:33705854-33705873 | MS.gene84222:CDS | 35.0% |
| ACCCATAGCATCTTTCTCAT+TGG | + | chr7.1:33705934-33705953 | None:intergenic | 40.0% | |
| TCTTGATATCGCCAAAGAAG+AGG | - | chr7.1:33705800-33705819 | MS.gene84222:CDS | 40.0% | |
| TTCTTTGGCGATATCAAGAG+TGG | + | chr7.1:33705799-33705818 | None:intergenic | 40.0% | |
| AAAACATTACGCCAAGGTGC+AGG | + | chr7.1:33705492-33705511 | None:intergenic | 45.0% | |
| AAGCTCCACGTCTTATGTCA+AGG | + | chr7.1:33705617-33705636 | None:intergenic | 45.0% | |
| AGCTCCACGTCTTATGTCAA+GGG | + | chr7.1:33705616-33705635 | None:intergenic | 45.0% | |
| CGCATCCTTAGGTGGAAAAT+CGG | + | chr7.1:33705532-33705551 | None:intergenic | 45.0% | |
| CGTGGTAAAACATTACGCCA+AGG | + | chr7.1:33705498-33705517 | None:intergenic | 45.0% | |
| TGTATGGCTTCAGAGGTGAA+TGG | - | chr7.1:33705742-33705761 | MS.gene84222:CDS | 45.0% | |
| TTGGCGATATCAAGAGTGGA+GGG | + | chr7.1:33705795-33705814 | None:intergenic | 45.0% | |
| TTTGGCGATATCAAGAGTGG+AGG | + | chr7.1:33705796-33705815 | None:intergenic | 45.0% | |
| ! | AGGTGAATGGCTTGATGACT+TGG | - | chr7.1:33705755-33705774 | MS.gene84222:CDS | 45.0% |
| ! | CAGTTTTCGCACCTCTTCTT+TGG | + | chr7.1:33705814-33705833 | None:intergenic | 45.0% |
| ! | GAATTTTCTGCTCGTCATGC+TGG | - | chr7.1:33705564-33705583 | MS.gene84222:CDS | 45.0% |
| !! | GAAACTTGTTGGAGAGTTGG+AGG | - | chr7.1:33705857-33705876 | MS.gene84222:CDS | 45.0% |
| !!! | TGTTGCCGATTTTCCACCTA+AGG | - | chr7.1:33705524-33705543 | MS.gene84222:CDS | 45.0% |
| !!! | TTTTTATTTTTATTTTGAAA+TGG | - | chr7.1:33705418-33705437 | MS.gene84222:CDS | 5.0% |
| AAATCGGCAACAACAGAGCG+TGG | + | chr7.1:33705516-33705535 | None:intergenic | 50.0% | |
| AGTGTGATCGCAAGAGCTTG+AGG | + | chr7.1:33705659-33705678 | None:intergenic | 50.0% | |
| CATCCCCTTGACATAAGACG+TGG | - | chr7.1:33705609-33705628 | MS.gene84222:CDS | 50.0% | |
| GACGAGCAGAAAATTCCCTG+AGG | + | chr7.1:33705560-33705579 | None:intergenic | 50.0% | |
| GCTCCACGTCTTATGTCAAG+GGG | + | chr7.1:33705615-33705634 | None:intergenic | 50.0% | |
| TCGCAAGAGCTTGAGGAAGT+TGG | + | chr7.1:33705652-33705671 | None:intergenic | 50.0% | |
| TCTTTACAGGTCCTGCACCT+TGG | - | chr7.1:33705478-33705497 | MS.gene84222:CDS | 50.0% | |
| ! | ACTTGTTGGAGAGTTGGAGG+AGG | - | chr7.1:33705860-33705879 | MS.gene84222:CDS | 50.0% |
| TAAGGATGCGCGACTCCTCA+GGG | - | chr7.1:33705542-33705561 | MS.gene84222:CDS | 55.0% | |
| AGGAGTCGCGCATCCTTAGG+TGG | + | chr7.1:33705540-33705559 | None:intergenic | 60.0% | |
| CTAAGGATGCGCGACTCCTC+AGG | - | chr7.1:33705541-33705560 | MS.gene84222:CDS | 60.0% | |
| CTGAGGAGTCGCGCATCCTT+AGG | + | chr7.1:33705543-33705562 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 33705356 | 33706022 | 33705356 | ID=MS.gene84222 |
| chr7.1 | mRNA | 33705356 | 33706022 | 33705356 | ID=MS.gene84222.t1;Parent=MS.gene84222 |
| chr7.1 | exon | 33706007 | 33706022 | 33706007 | ID=MS.gene84222.t1.exon1;Parent=MS.gene84222.t1 |
| chr7.1 | CDS | 33706007 | 33706022 | 33706007 | ID=cds.MS.gene84222.t1;Parent=MS.gene84222.t1 |
| chr7.1 | exon | 33705356 | 33705891 | 33705356 | ID=MS.gene84222.t1.exon2;Parent=MS.gene84222.t1 |
| chr7.1 | CDS | 33705356 | 33705891 | 33705356 | ID=cds.MS.gene84222.t1;Parent=MS.gene84222.t1 |
| Gene Sequence |
| Protein sequence |
