Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84726.t1 | XP_003627523.2 | 97 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 8.90E-124 | 453 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84726.t1 | G7LAU0 | 97.0 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 6.4e-124 | 453.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene84726 | 0.836246 | 1.05E-56 | -1.69E-46 |
| MS.gene050206 | MS.gene84726 | 0.827898 | 1.21E-54 | -1.69E-46 |
| MS.gene050563 | MS.gene84726 | 0.813953 | 1.97E-51 | -1.69E-46 |
| MS.gene050584 | MS.gene84726 | 0.852076 | 5.85E-61 | -1.69E-46 |
| MS.gene050585 | MS.gene84726 | 0.831718 | 1.42E-55 | -1.69E-46 |
| MS.gene050807 | MS.gene84726 | 0.827182 | 1.80E-54 | -1.69E-46 |
| MS.gene050808 | MS.gene84726 | 0.806459 | 8.14E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene84726 | 0.854246 | 1.40E-61 | -1.69E-46 |
| MS.gene052142 | MS.gene84726 | 0.821588 | 3.73E-53 | -1.69E-46 |
| MS.gene053074 | MS.gene84726 | 0.858303 | 9.04E-63 | -1.69E-46 |
| MS.gene053204 | MS.gene84726 | 0.804724 | 1.88E-49 | -1.69E-46 |
| MS.gene053383 | MS.gene84726 | 0.845877 | 3.09E-59 | -1.69E-46 |
| MS.gene053563 | MS.gene84726 | 0.802587 | 5.23E-49 | -1.69E-46 |
| MS.gene05456 | MS.gene84726 | 0.843307 | 1.52E-58 | -1.69E-46 |
| MS.gene054596 | MS.gene84726 | 0.811258 | 7.66E-51 | -1.69E-46 |
| MS.gene055359 | MS.gene84726 | 0.831242 | 1.87E-55 | -1.69E-46 |
| MS.gene059006 | MS.gene84726 | 0.869557 | 2.83E-66 | -1.69E-46 |
| MS.gene060513 | MS.gene84726 | 0.901477 | 2.58E-78 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84726.t1 | MTR_8g027100 | 97.021 | 235 | 7 | 0 | 1 | 235 | 1 | 235 | 8.56e-170 | 469 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84726.t1 | AT2G46640 | 34.759 | 187 | 103 | 9 | 1 | 184 | 1 | 171 | 4.58e-13 | 67.4 |
| MS.gene84726.t1 | AT2G46640 | 34.574 | 188 | 104 | 9 | 1 | 184 | 1 | 173 | 1.00e-11 | 63.9 |
| MS.gene84726.t1 | AT2G46640 | 34.574 | 188 | 104 | 9 | 1 | 184 | 1 | 173 | 1.10e-11 | 63.5 |
Find 36 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCAAAGCAAAGTATTCT+TGG | 0.268337 | 8.3:-67296660 | None:intergenic |
| GTATTCTTGGTGATTGTTCA+TGG | 0.327683 | 8.3:-67296647 | None:intergenic |
| CATGGTGTGATTTACCTTCA+TGG | 0.334098 | 8.3:-67296138 | None:intergenic |
| GAAGATTATTCCCCTTGTTA+AGG | 0.356621 | 8.3:+67297086 | MS.gene84726:CDS |
| GTTTGTTACAGATCTTCAAT+TGG | 0.412010 | 8.3:+67296298 | MS.gene84726:intron |
| AATGATGATTAGAAACTTCA+AGG | 0.418804 | 8.3:+67296855 | MS.gene84726:CDS |
| TCACCAAGAATACTTTGCTT+TGG | 0.419615 | 8.3:+67296657 | MS.gene84726:CDS |
| ATTCTTACCATTGGTACTCT+TGG | 0.429438 | 8.3:+67296607 | MS.gene84726:CDS |
| ACTTGACTCAACCAAGACTT+TGG | 0.432800 | 8.3:-67297019 | None:intergenic |
| TTTGTTACAGATCTTCAATT+GGG | 0.432814 | 8.3:+67296299 | MS.gene84726:intron |
| ACTTTCTTGATGTCTTTCAT+TGG | 0.437252 | 8.3:-67297120 | None:intergenic |
| AAACCGTTGAGAGTGTTGTT+AGG | 0.437651 | 8.3:-67296339 | None:intergenic |
| ATGATTTCATGACAAATCAT+AGG | 0.438088 | 8.3:-67296895 | None:intergenic |
| TCAAATGTGTTGTTGATCAA+TGG | 0.438577 | 8.3:-67296760 | None:intergenic |
| TTTCCAATCACCGAGCATAT+CGG | 0.445673 | 8.3:-67296578 | None:intergenic |
| TGACCGATATGCTCGGTGAT+TGG | 0.446300 | 8.3:+67296575 | MS.gene84726:CDS |
| ATTTGATGAGAACAACTTTG+AGG | 0.478699 | 8.3:+67296777 | MS.gene84726:CDS |
| TTATCAGTGTTGTTGATTGA+AGG | 0.480019 | 8.3:-67296526 | None:intergenic |
| TATGATTTGTCATGAAATCA+TGG | 0.489589 | 8.3:+67296897 | MS.gene84726:CDS |
| TTGAAGCTGATCAAAAGAAA+AGG | 0.490869 | 8.3:+67296932 | MS.gene84726:CDS |
| TGATGATGATGCTAGAAAAG+AGG | 0.505541 | 8.3:+67296828 | MS.gene84726:CDS |
| TTATTCTGCAGCCAAAGTCT+TGG | 0.511362 | 8.3:+67297008 | MS.gene84726:CDS |
| TTTCTATGATGTTGAACAAG+AGG | 0.511917 | 8.3:+67296729 | MS.gene84726:CDS |
| CTACATCATGCACTTCCTAA+AGG | 0.528996 | 8.3:+67297154 | MS.gene84726:CDS |
| TCTTCAATTGGGTGCACAAG+AGG | 0.532391 | 8.3:+67296310 | MS.gene84726:CDS |
| AAAGACGGAATTCTTACCAT+TGG | 0.543523 | 8.3:+67296598 | MS.gene84726:CDS |
| AAACTTCAAGGAAATTGTTG+AGG | 0.544282 | 8.3:+67296867 | MS.gene84726:CDS |
| CATCCTAACAACACTCTCAA+CGG | 0.551417 | 8.3:+67296336 | MS.gene84726:CDS |
| CTTCAAGGAAATTGTTGAGG+TGG | 0.564269 | 8.3:+67296870 | MS.gene84726:CDS |
| ATCATAGCCAAGAGTACCAA+TGG | 0.569824 | 8.3:-67296614 | None:intergenic |
| CATCATCATCAACTACATCA+TGG | 0.574853 | 8.3:-67296816 | None:intergenic |
| ATGCTCGGTGATTGGAAAGA+CGG | 0.581029 | 8.3:+67296583 | MS.gene84726:CDS |
| TGATTGTTCATGGATTTCAA+AGG | 0.604206 | 8.3:-67296637 | None:intergenic |
| GTTGCTTTGACCGATATGCT+CGG | 0.632441 | 8.3:+67296568 | MS.gene84726:CDS |
| TCTTAACATCAGAATCAGCA+AGG | 0.653265 | 8.3:-67296975 | None:intergenic |
| GATTTGTCATGAAATCATGG+AGG | 0.748556 | 8.3:+67296900 | MS.gene84726:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTTAGACACAAAATT+TGG | - | chr8.3:67296411-67296430 | None:intergenic | 15.0% |
| !!! | CACAATTTTTAAGACAATAA+AGG | - | chr8.3:67296226-67296245 | None:intergenic | 20.0% |
| ! | AATGATGATTAGAAACTTCA+AGG | + | chr8.3:67296855-67296874 | MS.gene84726:CDS | 25.0% |
| ! | ATGATTTCATGACAAATCAT+AGG | - | chr8.3:67296898-67296917 | None:intergenic | 25.0% |
| ! | CATATGCATGAGAAATATAA+TGG | - | chr8.3:67296178-67296197 | None:intergenic | 25.0% |
| ! | CCAAAACTTAAAGCAAAAAA+TGG | + | chr8.3:67297048-67297067 | MS.gene84726:CDS | 25.0% |
| ! | TTTGTTACAGATCTTCAATT+GGG | + | chr8.3:67296299-67296318 | MS.gene84726:intron | 25.0% |
| !! | TATGATTTGTCATGAAATCA+TGG | + | chr8.3:67296897-67296916 | MS.gene84726:CDS | 25.0% |
| !!! | CCATTTTTTGCTTTAAGTTT+TGG | - | chr8.3:67297051-67297070 | None:intergenic | 25.0% |
| !!! | GTTTGTTGTTTTTACTTAGA+TGG | + | chr8.3:67296475-67296494 | MS.gene84726:intron | 25.0% |
| AAACTTCAAGGAAATTGTTG+AGG | + | chr8.3:67296867-67296886 | MS.gene84726:CDS | 30.0% | |
| ACTTTCTTGATGTCTTTCAT+TGG | - | chr8.3:67297123-67297142 | None:intergenic | 30.0% | |
| ATTTGATGAGAACAACTTTG+AGG | + | chr8.3:67296777-67296796 | MS.gene84726:CDS | 30.0% | |
| TGATTGTTCATGGATTTCAA+AGG | - | chr8.3:67296640-67296659 | None:intergenic | 30.0% | |
| TTGAAGCTGATCAAAAGAAA+AGG | + | chr8.3:67296932-67296951 | MS.gene84726:CDS | 30.0% | |
| TTTCTATGATGTTGAACAAG+AGG | + | chr8.3:67296729-67296748 | MS.gene84726:CDS | 30.0% | |
| ! | GTTTGTTACAGATCTTCAAT+TGG | + | chr8.3:67296298-67296317 | MS.gene84726:intron | 30.0% |
| ! | TCAAATGTGTTGTTGATCAA+TGG | - | chr8.3:67296763-67296782 | None:intergenic | 30.0% |
| ! | TTATCAGTGTTGTTGATTGA+AGG | - | chr8.3:67296529-67296548 | None:intergenic | 30.0% |
| AAAGACGGAATTCTTACCAT+TGG | + | chr8.3:67296598-67296617 | MS.gene84726:CDS | 35.0% | |
| ATTCTTACCATTGGTACTCT+TGG | + | chr8.3:67296607-67296626 | MS.gene84726:CDS | 35.0% | |
| CATCATCATCAACTACATCA+TGG | - | chr8.3:67296819-67296838 | None:intergenic | 35.0% | |
| GAAGATTATTCCCCTTGTTA+AGG | + | chr8.3:67297086-67297105 | MS.gene84726:CDS | 35.0% | |
| GATTTGTCATGAAATCATGG+AGG | + | chr8.3:67296900-67296919 | MS.gene84726:CDS | 35.0% | |
| TCACCAAGAATACTTTGCTT+TGG | + | chr8.3:67296657-67296676 | MS.gene84726:CDS | 35.0% | |
| TCTTAACATCAGAATCAGCA+AGG | - | chr8.3:67296978-67296997 | None:intergenic | 35.0% | |
| ! | ACGTGCATTTTCCTTAACAA+GGG | - | chr8.3:67297100-67297119 | None:intergenic | 35.0% |
| ! | CTTCCAAAGCAAAGTATTCT+TGG | - | chr8.3:67296663-67296682 | None:intergenic | 35.0% |
| ! | GTATTCTTGGTGATTGTTCA+TGG | - | chr8.3:67296650-67296669 | None:intergenic | 35.0% |
| ! | TGATGATGATGCTAGAAAAG+AGG | + | chr8.3:67296828-67296847 | MS.gene84726:CDS | 35.0% |
| ! | TTTGGAACCTAAGTTGCAAA+TGG | - | chr8.3:67296393-67296412 | None:intergenic | 35.0% |
| ACTTGACTCAACCAAGACTT+TGG | - | chr8.3:67297022-67297041 | None:intergenic | 40.0% | |
| CATCCTAACAACACTCTCAA+CGG | + | chr8.3:67296336-67296355 | MS.gene84726:CDS | 40.0% | |
| CTTCAAGGAAATTGTTGAGG+TGG | + | chr8.3:67296870-67296889 | MS.gene84726:CDS | 40.0% | |
| TTATTCTGCAGCCAAAGTCT+TGG | + | chr8.3:67297008-67297027 | MS.gene84726:CDS | 40.0% | |
| TTTCCAATCACCGAGCATAT+CGG | - | chr8.3:67296581-67296600 | None:intergenic | 40.0% | |
| ! | CGTGCATTTTCCTTAACAAG+GGG | - | chr8.3:67297099-67297118 | None:intergenic | 40.0% |
| ! | GACGTGCATTTTCCTTAACA+AGG | - | chr8.3:67297101-67297120 | None:intergenic | 40.0% |
| !! | AAACCGTTGAGAGTGTTGTT+AGG | - | chr8.3:67296342-67296361 | None:intergenic | 40.0% |
| !! | ATCATAGCCAAGAGTACCAA+TGG | - | chr8.3:67296617-67296636 | None:intergenic | 40.0% |
| CTTCGCTCCATTTGCAACTT+AGG | + | chr8.3:67296383-67296402 | MS.gene84726:intron | 45.0% | |
| TCTTCAATTGGGTGCACAAG+AGG | + | chr8.3:67296310-67296329 | MS.gene84726:CDS | 45.0% | |
| ! | ATGCTCGGTGATTGGAAAGA+CGG | + | chr8.3:67296583-67296602 | MS.gene84726:CDS | 45.0% |
| ! | GTTGCTTTGACCGATATGCT+CGG | + | chr8.3:67296568-67296587 | MS.gene84726:CDS | 45.0% |
| ATGGCTGTGTTGTGTGTGCA+TGG | - | chr8.3:67296159-67296178 | None:intergenic | 50.0% | |
| ! | TGACCGATATGCTCGGTGAT+TGG | + | chr8.3:67296575-67296594 | MS.gene84726:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 67296140 | 67297173 | 67296140 | ID=MS.gene84726 |
| chr8.3 | mRNA | 67296140 | 67297173 | 67296140 | ID=MS.gene84726.t1;Parent=MS.gene84726 |
| chr8.3 | exon | 67296140 | 67296145 | 67296140 | ID=MS.gene84726.t1.exon1;Parent=MS.gene84726.t1 |
| chr8.3 | CDS | 67296140 | 67296145 | 67296140 | ID=cds.MS.gene84726.t1;Parent=MS.gene84726.t1 |
| chr8.3 | exon | 67296309 | 67296357 | 67296309 | ID=MS.gene84726.t1.exon2;Parent=MS.gene84726.t1 |
| chr8.3 | CDS | 67296309 | 67296357 | 67296309 | ID=cds.MS.gene84726.t1;Parent=MS.gene84726.t1 |
| chr8.3 | exon | 67296494 | 67297173 | 67296494 | ID=MS.gene84726.t1.exon3;Parent=MS.gene84726.t1 |
| chr8.3 | CDS | 67296494 | 67297173 | 67296494 | ID=cds.MS.gene84726.t1;Parent=MS.gene84726.t1 |
| Gene Sequence |
| Protein sequence |
