AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84948


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84948.t1 MTR_1g033420 85.135 74 9 1 1 72 103 176 1.29e-39 133
MS.gene84948.t1 MTR_5g029100 66.216 74 23 1 1 72 81 154 2.09e-29 106
MS.gene84948.t1 MTR_5g029100 69.697 66 20 0 1 66 112 177 6.49e-29 105
MS.gene84948.t1 MTR_8g090100 63.492 63 21 1 4 66 97 157 3.36e-23 90.1
MS.gene84948.t1 MTR_8g064180 53.846 65 30 0 2 66 117 181 1.44e-21 85.9
MS.gene84948.t1 MTR_4g045853 53.247 77 30 3 5 78 96 169 2.73e-20 82.0
MS.gene84948.t1 MTR_2g089140 50.000 76 34 2 5 78 94 167 3.53e-20 81.6
MS.gene84948.t1 MTR_2g089140 50.000 76 34 2 5 78 31 104 4.88e-20 80.5
MS.gene84948.t1 MTR_4g057450 55.263 76 29 2 2 72 119 194 7.55e-20 80.9
MS.gene84948.t1 MTR_2g095800 55.882 68 27 1 2 66 123 190 1.53e-19 80.5
MS.gene84948.t1 MTR_4g128570 50.649 77 34 2 5 79 102 176 7.08e-19 78.2
MS.gene84948.t1 MTR_4g097700 52.000 75 32 2 7 79 143 215 1.23e-18 78.2
MS.gene84948.t1 MTR_8g102220 46.250 80 39 2 6 83 101 178 1.35e-18 77.4
MS.gene84948.t1 MTR_1g105495 53.623 69 30 1 7 75 111 177 1.72e-18 77.4
MS.gene84948.t1 MTR_1g105900 50.685 73 32 2 3 73 103 173 1.82e-18 77.4
MS.gene84948.t1 MTR_4g097640 52.000 75 32 2 7 79 106 178 1.89e-18 77.0
MS.gene84948.t1 MTR_4g097670 52.000 75 32 2 7 79 106 178 1.89e-18 77.0
MS.gene84948.t1 MTR_8g013055 52.113 71 30 2 5 73 102 170 5.26e-18 75.9
MS.gene84948.t1 MTR_4g126920 50.725 69 30 2 6 72 97 163 1.12e-17 75.1
MS.gene84948.t1 MTR_4g128580 49.367 79 36 2 7 83 99 175 1.49e-17 74.7
MS.gene84948.t1 MTR_7g093530 49.367 79 36 2 7 83 97 173 1.78e-17 74.3
MS.gene84948.t1 MTR_4g128590 52.941 68 28 2 7 72 88 153 1.81e-17 74.3
MS.gene84948.t1 MTR_5g091730 56.667 60 24 1 6 65 100 157 3.89e-17 73.6
MS.gene84948.t1 MTR_7g102260 51.389 72 30 2 6 72 110 181 6.35e-17 73.2
MS.gene84948.t1 MTR_3g084620 45.000 80 40 2 6 83 100 177 8.81e-17 72.4
MS.gene84948.t1 MTR_3g084630 45.000 80 40 2 6 83 100 177 9.18e-17 72.4
MS.gene84948.t1 MTR_7g084770 52.857 70 29 2 6 73 104 171 1.06e-16 72.4
MS.gene84948.t1 MTR_7g084760 52.857 70 29 2 6 73 104 171 1.06e-16 72.4
MS.gene84948.t1 MTR_3g089540 47.500 80 38 2 6 83 102 179 1.74e-16 72.0
MS.gene84948.t1 MTR_7g056090 55.556 63 25 2 4 66 103 162 2.03e-16 71.6
MS.gene84948.t1 MTR_4g128410 48.101 79 37 2 7 83 98 174 2.11e-16 71.6
MS.gene84948.t1 MTR_8g081040 45.000 80 40 2 6 83 103 180 2.82e-16 71.2
MS.gene84948.t1 MTR_7g084750 49.296 71 32 2 5 73 103 171 3.11e-16 71.2
MS.gene84948.t1 MTR_6g088320 47.143 70 33 2 6 73 96 163 5.46e-16 70.5
MS.gene84948.t1 MTR_2g038860 46.250 80 39 2 6 83 98 175 6.48e-16 70.5
MS.gene84948.t1 MTR_2g038705 46.250 80 39 2 6 83 98 175 6.48e-16 70.5
MS.gene84948.t1 MTR_6g033085 50.000 70 31 2 6 73 104 171 7.50e-16 70.1
MS.gene84948.t1 MTR_1g063020 51.562 64 28 1 6 66 113 176 8.33e-16 70.1
MS.gene84948.t1 MTR_6g033055 50.000 70 31 2 6 73 104 171 1.15e-15 69.7
MS.gene84948.t1 MTR_8g102910 47.143 70 33 2 6 73 92 159 1.45e-15 69.3
MS.gene84948.t1 MTR_4g128400 44.304 79 40 2 7 83 101 177 1.10e-14 67.0
MS.gene84948.t1 MTR_2g049030 48.571 70 31 3 7 73 101 168 8.23e-14 64.7
MS.gene84948.t1 MTR_2g049130 43.478 69 35 2 7 73 103 169 1.57e-13 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84948.t1 AT1G14720 78.378 74 14 1 1 72 101 174 4.22e-37 127
MS.gene84948.t1 AT2G01850 75.309 81 17 2 1 78 101 181 1.22e-35 123
MS.gene84948.t1 AT1G32170 64.198 81 27 1 1 79 102 182 1.45e-30 110
MS.gene84948.t1 AT4G18990 64.865 74 24 1 1 72 36 109 1.53e-29 106
MS.gene84948.t1 AT4G18990 64.865 74 24 1 1 72 110 183 1.14e-28 105
MS.gene84948.t1 AT1G10550 55.385 65 29 0 2 66 112 176 4.69e-23 89.7
MS.gene84948.t1 AT2G14620 53.425 73 32 1 3 75 45 115 4.01e-20 80.9
MS.gene84948.t1 AT2G14620 53.425 73 32 1 3 75 56 126 4.30e-20 80.9
MS.gene84948.t1 AT2G14620 53.425 73 32 1 3 75 106 176 7.25e-20 81.3
MS.gene84948.t1 AT4G03210 50.000 76 34 2 5 78 70 143 3.91e-19 78.6
MS.gene84948.t1 AT4G37800 49.367 79 36 2 7 83 108 184 4.12e-19 79.0
MS.gene84948.t1 AT4G03210 50.000 76 34 2 5 78 98 171 5.43e-19 78.6
MS.gene84948.t1 AT3G44990 50.000 74 32 2 5 73 111 184 1.50e-18 77.4
MS.gene84948.t1 AT1G11545 47.436 78 39 1 7 84 114 189 2.95e-18 77.0
MS.gene84948.t1 AT4G25810 52.000 75 32 2 7 79 99 171 3.92e-18 76.3
MS.gene84948.t1 AT2G36870 56.250 64 25 1 6 66 116 179 6.35e-18 75.9
MS.gene84948.t1 AT5G13870 52.857 70 29 2 6 73 104 171 6.70e-18 75.9
MS.gene84948.t1 AT5G13870 52.857 70 29 2 6 73 104 171 6.70e-18 75.9
MS.gene84948.t1 AT5G13870 52.857 70 29 2 6 73 104 171 6.70e-18 75.9
MS.gene84948.t1 AT5G48070 48.101 79 37 2 7 83 103 179 1.22e-17 75.1
MS.gene84948.t1 AT4G13090 54.286 70 28 2 6 73 104 171 1.28e-17 75.1
MS.gene84948.t1 AT4G30290 48.101 79 37 2 7 83 98 174 2.82e-17 73.9
MS.gene84948.t1 AT5G57560 48.684 76 35 2 7 80 96 169 3.84e-17 73.6
MS.gene84948.t1 AT3G23730 47.500 80 38 2 6 83 99 176 4.51e-17 73.6
MS.gene84948.t1 AT4G14130 47.500 80 38 2 6 83 100 177 5.38e-17 73.2
MS.gene84948.t1 AT2G36870 53.968 63 26 1 6 65 116 178 6.26e-17 71.2
MS.gene84948.t1 AT5G65730 48.529 68 31 2 7 72 109 174 1.00e-16 72.4
MS.gene84948.t1 AT1G65310 48.101 79 37 2 7 83 125 201 1.09e-16 72.8
MS.gene84948.t1 AT1G65310 48.101 79 37 2 7 83 103 179 1.12e-16 72.4
MS.gene84948.t1 AT5G57530 50.667 75 33 2 7 79 100 172 1.32e-16 72.0
MS.gene84948.t1 AT5G57550 53.333 60 26 1 7 66 103 160 1.68e-16 72.0
MS.gene84948.t1 AT4G30280 51.471 68 29 2 7 72 103 168 1.88e-16 71.6
MS.gene84948.t1 AT2G18800 52.941 68 28 2 7 72 101 166 2.72e-16 71.6
MS.gene84948.t1 AT5G57540 49.333 75 34 2 7 79 99 171 3.14e-16 71.2
MS.gene84948.t1 AT4G13080 50.000 70 30 3 6 73 111 177 5.36e-16 70.5
MS.gene84948.t1 AT3G25050 45.000 80 40 2 6 83 107 184 9.50e-16 70.1
MS.gene84948.t1 AT4G30270 53.333 60 26 1 7 66 97 154 1.13e-15 69.3
MS.gene84948.t1 AT2G06850 50.000 70 31 2 6 73 47 114 2.36e-15 68.2
MS.gene84948.t1 AT2G06850 50.000 70 31 2 6 73 107 174 2.68e-15 68.9
MS.gene84948.t1 AT4G28850 45.714 70 34 2 6 73 99 166 3.79e-15 68.2
MS.gene84948.t1 AT4G25820 50.725 69 30 2 7 73 103 169 1.36e-14 66.6
MS.gene84948.t1 AT3G48580 40.000 70 37 3 6 73 46 112 2.64e-11 57.0
MS.gene84948.t1 AT3G48580 40.000 70 37 3 6 73 105 171 5.55e-11 56.6

Find 22 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATTCAGACCAATGTTTA+TGG 0.234371 1.3:-37011873 MS.gene84948:CDS
CAAAGGCTTCCATGGTGATT+TGG 0.307852 1.3:+37010963 None:intergenic
ACAGATTTAAACAGATTTGA+TGG 0.310159 1.3:-37011001 MS.gene84948:intron
GAGAGGAAAGATATGGTCTT+TGG 0.319574 1.3:-37011827 MS.gene84948:CDS
GAAACATTAGAGGCAAAGAT+TGG 0.421060 1.3:-37011896 MS.gene84948:CDS
TTCATCAATACAGTATTCTC+TGG 0.426009 1.3:-37011785 MS.gene84948:CDS
AGCCTTTGAACAACCTGGTT+AGG 0.459403 1.3:-37010948 None:intergenic
TCTTTCCTCTCTGCCAATGC+TGG 0.468353 1.3:+37011839 None:intergenic
CAGACCAATGTTTATGGCAA+TGG 0.496594 1.3:-37011867 MS.gene84948:CDS
GTATTCTTTGGAACCTAACC+AGG 0.498379 1.3:+37010935 None:intergenic
ACTCAAAATCTATTTCATCA+TGG 0.506747 1.3:+37011925 None:intergenic
GGCAATGGAAGCACCAGCAT+TGG 0.516287 1.3:-37011852 MS.gene84948:CDS
AGGTTGTTCAAAGGCTTCCA+TGG 0.521110 1.3:+37010955 None:intergenic
TATTGATGAAAATCCTCAGC+AGG 0.535202 1.3:+37011798 None:intergenic
ACATTAGAGGCAAAGATTGG+AGG 0.561664 1.3:-37011893 MS.gene84948:CDS
GCTTCCATTGCCATAAACAT+TGG 0.568127 1.3:+37011863 None:intergenic
ATTGGCAGAGAGGAAAGATA+TGG 0.574281 1.3:-37011834 MS.gene84948:CDS
AACCTAACCAGGTTGTTCAA+AGG 0.578288 1.3:+37010946 None:intergenic
TCTTTGGTTTGATCCTGCTG+AGG 0.596535 1.3:-37011811 MS.gene84948:CDS
ATGGAAGCCTTTGAACAACC+TGG 0.600912 1.3:-37010953 MS.gene84948:CDS
AATCAAAGTCCAAATCACCA+TGG 0.671373 1.3:-37010972 MS.gene84948:CDS
AAGCACCAGCATTGGCAGAG+AGG 0.671848 1.3:-37011844 MS.gene84948:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATAGATTTTGAGTTTTT+GGG - chr1.3:37010970-37010989 MS.gene84948:CDS 20.0%
!!! TGAAATAGATTTTGAGTTTT+TGG - chr1.3:37010969-37010988 MS.gene84948:CDS 20.0%
! AAAATTAGTTAAGAAGATGC+AGG + chr1.3:37011228-37011247 None:intergenic 25.0%
! ACAGATTTAAACAGATTTGA+TGG - chr1.3:37011887-37011906 MS.gene84948:CDS 25.0%
! ACTCAAAATCTATTTCATCA+TGG + chr1.3:37010966-37010985 None:intergenic 25.0%
! AGATGCAAGTAAATATTTGT+CGG + chr1.3:37011698-37011717 None:intergenic 25.0%
! ATTTAGTTGTTTATCCAAAC+AGG + chr1.3:37011477-37011496 None:intergenic 25.0%
! CATACATACAATACAATATC+TGG - chr1.3:37011168-37011187 MS.gene84948:intron 25.0%
! GATATTGTATTGTATGTATG+AGG + chr1.3:37011168-37011187 None:intergenic 25.0%
! TAAGTTACTACCTATAAACA+AGG + chr1.3:37011331-37011350 None:intergenic 25.0%
! TATTGTAAGGCTATTATGTA+AGG - chr1.3:37011846-37011865 MS.gene84948:CDS 25.0%
! TTTAGTTGTTTATCCAAACA+GGG + chr1.3:37011476-37011495 None:intergenic 25.0%
! TTTGGTATGTAAAAATCCAA+AGG + chr1.3:37011198-37011217 None:intergenic 25.0%
!! AATAAGTTGAAATCAGCTTA+TGG - chr1.3:37011633-37011652 MS.gene84948:intron 25.0%
!! ATCATCACTTAAGTATTGTA+AGG - chr1.3:37011833-37011852 MS.gene84948:CDS 25.0%
CAAGGAATCTATATATGACT+TGG + chr1.3:37011426-37011445 None:intergenic 30.0%
GATGCAAGTAAATATTTGTC+GGG + chr1.3:37011697-37011716 None:intergenic 30.0%
TGAAGATGCTTAAAATCTGT+CGG - chr1.3:37011730-37011749 MS.gene84948:intron 30.0%
TTCATCAATACAGTATTCTC+TGG - chr1.3:37011103-37011122 MS.gene84948:intron 30.0%
! CAATAACGATCCTTGTTTAT+AGG - chr1.3:37011318-37011337 MS.gene84948:intron 30.0%
!!! ATCTAGCTGTTTTTTGTTGT+TGG - chr1.3:37011351-37011370 MS.gene84948:intron 30.0%
!!! TCTAGCTGTTTTTTGTTGTT+GGG - chr1.3:37011352-37011371 MS.gene84948:intron 30.0%
AAGGATCGTTATTGCATGAA+AGG + chr1.3:37011312-37011331 None:intergenic 35.0%
AATCAAAGTCCAAATCACCA+TGG - chr1.3:37011916-37011935 MS.gene84948:CDS 35.0%
AGGATTCAGACCAATGTTTA+TGG - chr1.3:37011015-37011034 MS.gene84948:intron 35.0%
ATTTGATTATAAGCCCCTGA+TGG + chr1.3:37011815-37011834 None:intergenic 35.0%
CAACCAATCAAACATTCACA+CGG + chr1.3:37011770-37011789 None:intergenic 35.0%
GAAACATTAGAGGCAAAGAT+TGG - chr1.3:37010992-37011011 MS.gene84948:intron 35.0%
GAAAGGCAAAAGTGAAATGA+TGG + chr1.3:37011295-37011314 None:intergenic 35.0%
GAAGATGCAGGTATATTCTT+TGG + chr1.3:37011216-37011235 None:intergenic 35.0%
GTACAACCTATTAAAACAGC+TGG + chr1.3:37011598-37011617 None:intergenic 35.0%
TAAATATTTGTCGGGCTATC+TGG + chr1.3:37011689-37011708 None:intergenic 35.0%
TATTGATGAAAATCCTCAGC+AGG + chr1.3:37011093-37011112 None:intergenic 35.0%
TTACCGTGTGAATGTTTGAT+TGG - chr1.3:37011764-37011783 MS.gene84948:intron 35.0%
! TACAATATCTGGTTCACCTT+TGG - chr1.3:37011179-37011198 MS.gene84948:intron 35.0%
!! GAGTTTTTGGGAAACATTAG+AGG - chr1.3:37010982-37011001 MS.gene84948:CDS 35.0%
!!! AAGCTACCAGCTGTTTTAAT+AGG - chr1.3:37011589-37011608 MS.gene84948:intron 35.0%
AATCCATATCCTACCATCAG+GGG - chr1.3:37011799-37011818 MS.gene84948:CDS 40.0%
ACATTAGAGGCAAAGATTGG+AGG - chr1.3:37010995-37011014 MS.gene84948:intron 40.0%
ATTGGCAGAGAGGAAAGATA+TGG - chr1.3:37011054-37011073 MS.gene84948:intron 40.0%
CAGACCAATGTTTATGGCAA+TGG - chr1.3:37011021-37011040 MS.gene84948:intron 40.0%
CGTGTGAATGTTTGATTGGT+TGG - chr1.3:37011768-37011787 MS.gene84948:CDS 40.0%
GAATCCATATCCTACCATCA+GGG - chr1.3:37011798-37011817 MS.gene84948:CDS 40.0%
GAGAGGAAAGATATGGTCTT+TGG - chr1.3:37011061-37011080 MS.gene84948:intron 40.0%
GCTTCCATTGCCATAAACAT+TGG + chr1.3:37011028-37011047 None:intergenic 40.0%
! ATGTTTGATTGGTTGGCAGT+AGG - chr1.3:37011775-37011794 MS.gene84948:CDS 40.0%
! TGTTTGATTGGTTGGCAGTA+GGG - chr1.3:37011776-37011795 MS.gene84948:CDS 40.0%
AACAGGGCAAATCGAATGTG+TGG + chr1.3:37011460-37011479 None:intergenic 45.0%
AGGTTGTACTTGCGAGCTTA+CGG - chr1.3:37011609-37011628 MS.gene84948:intron 45.0%
AGGTTGTTCAAAGGCTTCCA+TGG + chr1.3:37011936-37011955 None:intergenic 45.0%
ATGGAAGCCTTTGAACAACC+TGG - chr1.3:37011935-37011954 MS.gene84948:CDS 45.0%
CAAAGGCTTCCATGGTGATT+TGG + chr1.3:37011928-37011947 None:intergenic 45.0%
GATTATAAGCCCCTGATGGT+AGG + chr1.3:37011811-37011830 None:intergenic 45.0%
GGAATCCATATCCTACCATC+AGG - chr1.3:37011797-37011816 MS.gene84948:CDS 45.0%
! CACATTCGATTTGCCCTGTT+TGG - chr1.3:37011460-37011479 MS.gene84948:intron 45.0%
!! TCTTTGGTTTGATCCTGCTG+AGG - chr1.3:37011077-37011096 MS.gene84948:intron 45.0%
AAGCCCCTGATGGTAGGATA+TGG + chr1.3:37011805-37011824 None:intergenic 50.0%
TCTTTCCTCTCTGCCAATGC+TGG + chr1.3:37011052-37011071 None:intergenic 50.0%
TGTGTGGTCTCTTGACAGCA+AGG + chr1.3:37011444-37011463 None:intergenic 50.0%
!! AAGCACCAGCATTGGCAGAG+AGG - chr1.3:37011044-37011063 MS.gene84948:intron 55.0%
!! GGCAATGGAAGCACCAGCAT+TGG - chr1.3:37011036-37011055 MS.gene84948:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 37010949 37011961 37010949 ID=MS.gene84948
chr1.3 mRNA 37010949 37011961 37010949 ID=MS.gene84948.t1;Parent=MS.gene84948
chr1.3 exon 37011768 37011961 37011768 ID=MS.gene84948.t1.exon1;Parent=MS.gene84948.t1
chr1.3 CDS 37011768 37011961 37011768 ID=cds.MS.gene84948.t1;Parent=MS.gene84948.t1
chr1.3 exon 37010949 37011009 37010949 ID=MS.gene84948.t1.exon2;Parent=MS.gene84948.t1
chr1.3 CDS 37010949 37011009 37010949 ID=cds.MS.gene84948.t1;Parent=MS.gene84948.t1
Gene Sequence

>MS.gene84948

ATGTATGAGAAGAACCATGATGAAATAGATTTTGAGTTTTTGGGAAACATTAGAGGCAAAGATTGGAGGATTCAGACCAATGTTTATGGCAATGGAAGCACCAGCATTGGCAGAGAGGAAAGATATGGTCTTTGGTTTGATCCTGCTGAGGATTTTCATCAATACAGTATTCTCTGGACATATTCTCGTATCATGTAAGTTTCTATCTTTCTTGAACCTCATACATACAATACAATATCTGGTTCACCTTTGGATTTTTACATACCAAAGAATATACCTGCATCTTCTTAACTAATTTTTTTTTCATCATTTCACTTTTAGCTTTCATGCAGTAATTTTTTTTCCATCATTTCACTTTTGCCTTTCATGCAATAACGATCCTTGTTTATAGGTAGTAACTTAATCTAGCTGTTTTTTGTTGTTGGGTTTGATGTGCTTTGTTCAAAGATTTCTCTGATTTTTTTACTTCTTTTACCAAGTCATATATAGATTCCTTGCTGTCAAGAGACCACACATTCGATTTGCCCTGTTTGGATAAACAACTAAATTAAGCACGCTTATAACATAAACACTTAACATATACAATGCTTATATCTAAGCTTTTTCTGTAACAAAACAAAGTAAAAAAAGATTTTCAAATAAGCTACCAGCTGTTTTAATAGGTTGTACTTGCGAGCTTACGGAAATAAGTTGAAATCAGCTTATGGACATGTCATAAGTTGTTGACATAAGCTCTTCCAGATAGCCCGACAAATATTTACTTGCATCTTTCTGTTGAAATTGAAGATGCTTAAAATCTGTCGGCGTTAATTCACTTACCGTGTGAATGTTTGATTGGTTGGCAGTAGGGAATCCATATCCTACCATCAGGGGCTTATAATCAAATCATCACTTAAGTATTGTAAGGCTATTATGTAAGGCTATCTCAAACAATTTAAACAGATTTAAACAGATTTGATGGTGTAAGAATCAAAGTCCAAATCACCATGGAAGCCTTTGAACAACCTGGTTAG

Protein sequence

>MS.gene84948.t1

MYEKNHDEIDFEFLGNIRGKDWRIQTNVYGNGSTSIGREERYGLWFDPAEDFHQYSILWTYSRIIFDGVRIKVQITMEAFEQPG