Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87399.t1 | XP_003629502.1 | 97.9 | 144 | 3 | 0 | 1 | 144 | 334 | 477 | 3.50E-71 | 277.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87399.t1 | G7LFJ1 | 97.9 | 144 | 3 | 0 | 1 | 144 | 334 | 477 | 2.5e-71 | 277.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049703 | MS.gene87399 | -0.804794 | 1.82E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene87399 | 0.818666 | 1.74E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene87399 | 0.821813 | 3.31E-53 | -1.69E-46 |
| MS.gene051063 | MS.gene87399 | 0.814688 | 1.36E-51 | -1.69E-46 |
| MS.gene051746 | MS.gene87399 | 0.855676 | 5.38E-62 | -1.69E-46 |
| MS.gene051748 | MS.gene87399 | 0.860289 | 2.29E-63 | -1.69E-46 |
| MS.gene051756 | MS.gene87399 | 0.830887 | 2.28E-55 | -1.69E-46 |
| MS.gene051757 | MS.gene87399 | 0.807471 | 4.97E-50 | -1.69E-46 |
| MS.gene051850 | MS.gene87399 | 0.815892 | 7.32E-52 | -1.69E-46 |
| MS.gene051903 | MS.gene87399 | 0.81356 | 2.41E-51 | -1.69E-46 |
| MS.gene052335 | MS.gene87399 | 0.800289 | 1.55E-48 | -1.69E-46 |
| MS.gene054250 | MS.gene87399 | 0.815472 | 9.09E-52 | -1.69E-46 |
| MS.gene056432 | MS.gene87399 | 0.803469 | 3.44E-49 | -1.69E-46 |
| MS.gene057057 | MS.gene87399 | 0.816413 | 5.60E-52 | -1.69E-46 |
| MS.gene057094 | MS.gene87399 | 0.818257 | 2.15E-52 | -1.69E-46 |
| MS.gene057234 | MS.gene87399 | 0.81415 | 1.78E-51 | -1.69E-46 |
| MS.gene058758 | MS.gene87399 | 0.803688 | 3.09E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene87399 | 0.80093 | 1.14E-48 | -1.69E-46 |
| MS.gene059171 | MS.gene87399 | 0.819193 | 1.32E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene87399 | 0.807373 | 5.22E-50 | -1.69E-46 |
| MS.gene060465 | MS.gene87399 | 0.806512 | 7.94E-50 | -1.69E-46 |
| MS.gene060702 | MS.gene87399 | 0.833534 | 5.05E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87399.t1 | MTR_8g078230 | 97.917 | 144 | 3 | 0 | 1 | 144 | 334 | 477 | 9.01e-100 | 296 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87399.t1 | AT1G53645 | 58.904 | 146 | 58 | 2 | 1 | 144 | 353 | 498 | 1.13e-53 | 177 |
Find 32 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCTTTCTCATCAATATC+TGG | 0.279645 | 8.3:-25960961 | None:intergenic |
| CCGTGCTGTTATCTCTCTTC+AGG | 0.286733 | 8.3:+25961577 | MS.gene87399:CDS |
| TCAAACTCCACCGCATATTC+TGG | 0.299644 | 8.3:-25960934 | None:intergenic |
| ACGGAAGATGGTGCACTTGT+AGG | 0.363935 | 8.3:-25961539 | None:intergenic |
| GAAAGAACCAATTGCACTTC+GGG | 0.373063 | 8.3:+25960975 | MS.gene87399:CDS |
| TTCTTTGCAGTTACCCTATC+CGG | 0.384971 | 8.3:-25961479 | None:intergenic |
| CTCATTATGAGTGTGTCTGC+TGG | 0.402597 | 8.3:+25961671 | MS.gene87399:CDS |
| AATTGAAGAGTTAATGCAAC+GGG | 0.439485 | 8.3:+25961418 | MS.gene87399:CDS |
| AGTGCATCCCGAAGTGCAAT+TGG | 0.458897 | 8.3:-25960982 | None:intergenic |
| TCGGATTACTGAAGCATATG+AGG | 0.482732 | 8.3:+25960599 | MS.gene87399:CDS |
| GTGAACTCCTTCACGGAAGA+TGG | 0.511944 | 8.3:-25961551 | None:intergenic |
| TCACTACAGCGGACCGGATA+GGG | 0.530780 | 8.3:+25961466 | MS.gene87399:CDS |
| AGAAAGAACCAATTGCACTT+CGG | 0.541606 | 8.3:+25960974 | MS.gene87399:CDS |
| ACGGTTAGTGAACTCCTTCA+CGG | 0.554765 | 8.3:-25961558 | None:intergenic |
| ATCACTACAGCGGACCGGAT+AGG | 0.569748 | 8.3:+25961465 | MS.gene87399:CDS |
| GATTCGAAGTCAAGAAGAGT+GGG | 0.572829 | 8.3:+25961041 | MS.gene87399:CDS |
| GGATTCGAAGTCAAGAAGAG+TGG | 0.586087 | 8.3:+25961040 | MS.gene87399:CDS |
| ACCGTTGCAAGATGAATATG+TGG | 0.589115 | 8.3:+25960647 | MS.gene87399:CDS |
| TCCACATATTCATCTTGCAA+CGG | 0.589778 | 8.3:-25960648 | None:intergenic |
| TATTCATCTTGCAACGGTGA+GGG | 0.599373 | 8.3:-25960642 | None:intergenic |
| TGTTGATCACTACAGCGGAC+CGG | 0.602028 | 8.3:+25961460 | MS.gene87399:CDS |
| ATATTCATCTTGCAACGGTG+AGG | 0.616062 | 8.3:-25960643 | None:intergenic |
| AGATGAATATGTGGAAGCAA+TGG | 0.616565 | 8.3:+25960656 | MS.gene87399:CDS |
| TTGGGCCTGAGATGATGGAT+CGG | 0.619513 | 8.3:+25960580 | None:intergenic |
| TGAATTTGAGCCAGAATATG+CGG | 0.624630 | 8.3:+25960924 | MS.gene87399:CDS |
| AAGTGCACCATCTTCCGTGA+AGG | 0.642415 | 8.3:+25961544 | MS.gene87399:CDS |
| ATTTGAGCCAGAATATGCGG+TGG | 0.671575 | 8.3:+25960927 | MS.gene87399:CDS |
| TAATTGAAGAGTTAATGCAA+CGG | 0.672734 | 8.3:+25961417 | MS.gene87399:CDS |
| TCGAAGTCAAGAAGAGTGGG+AGG | 0.683559 | 8.3:+25961044 | MS.gene87399:CDS |
| ACACTCATAATGAGAAACAA+CGG | 0.696606 | 8.3:-25961662 | None:intergenic |
| AAGATTGTTGATCACTACAG+CGG | 0.722275 | 8.3:+25961455 | MS.gene87399:CDS |
| CCTGAAGAGAGATAACAGCA+CGG | 0.771811 | 8.3:-25961577 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATAAAATGCTTTTACTT+AGG | + | chr8.3:25961639-25961658 | MS.gene87399:intron | 15.0% |
| !!! | TAGGATTTTATTTTATTTCT+AGG | + | chr8.3:25960755-25960774 | MS.gene87399:intron | 15.0% |
| !! | AACAAAAAGATAGAGATATA+TGG | - | chr8.3:25960710-25960729 | None:intergenic | 20.0% |
| !! | AAGTTATGCCATTATATTAT+TGG | - | chr8.3:25961115-25961134 | None:intergenic | 20.0% |
| !! | AGTTATGCCATTATATTATT+GGG | - | chr8.3:25961114-25961133 | None:intergenic | 20.0% |
| !! | GTTAACTAAAACAAAGTTAT+CGG | - | chr8.3:25961229-25961248 | None:intergenic | 20.0% |
| !! | TTGTAACTGTAAATATTGTT+GGG | + | chr8.3:25961601-25961620 | MS.gene87399:intron | 20.0% |
| !! | TTTGTAACTGTAAATATTGT+TGG | + | chr8.3:25961600-25961619 | MS.gene87399:intron | 20.0% |
| !!! | ATATATCTCTATCTTTTTGT+TGG | + | chr8.3:25960709-25960728 | MS.gene87399:intron | 20.0% |
| !!! | TTGGAATAGTTGTATTTTAT+GGG | + | chr8.3:25961296-25961315 | MS.gene87399:intron | 20.0% |
| !!! | TTTGGAATAGTTGTATTTTA+TGG | + | chr8.3:25961295-25961314 | MS.gene87399:intron | 20.0% |
| ! | ATCTATATGTGCAAGTATAT+TGG | + | chr8.3:25961357-25961376 | MS.gene87399:intron | 25.0% |
| ! | ATTACTGCCCAATAATATAA+TGG | + | chr8.3:25961104-25961123 | MS.gene87399:intron | 25.0% |
| ! | TAATTGAAGAGTTAATGCAA+CGG | + | chr8.3:25961417-25961436 | MS.gene87399:CDS | 25.0% |
| ! | TATCTTCACTAACAATCTTA+TGG | + | chr8.3:25961195-25961214 | MS.gene87399:intron | 25.0% |
| !!! | TCAACAATCTTTTTCAACAA+TGG | - | chr8.3:25961446-25961465 | None:intergenic | 25.0% |
| !!! | TTGCCATTTTTCATAAAGAA+CGG | + | chr8.3:25960800-25960819 | MS.gene87399:intron | 25.0% |
| AATTGAAGAGTTAATGCAAC+GGG | + | chr8.3:25961418-25961437 | MS.gene87399:CDS | 30.0% | |
| ACACTCATAATGAGAAACAA+CGG | - | chr8.3:25961665-25961684 | None:intergenic | 30.0% | |
| TTAACTCTTCAATTACTTCC+TGG | - | chr8.3:25961412-25961431 | None:intergenic | 30.0% | |
| TTCCCGTTCTTTATGAAAAA+TGG | - | chr8.3:25960806-25960825 | None:intergenic | 30.0% | |
| ! | CTGTATTGCTGCATTTATTT+TGG | + | chr8.3:25961277-25961296 | MS.gene87399:intron | 30.0% |
| ! | TATAATGGCATAACTTGCAA+TGG | + | chr8.3:25961119-25961138 | MS.gene87399:intron | 30.0% |
| ! | TGATTCTAAGAAGTGGAAAT+AGG | + | chr8.3:25960736-25960755 | MS.gene87399:intron | 30.0% |
| ! | TGGAAAATGATTCTAAGAAG+TGG | + | chr8.3:25960729-25960748 | MS.gene87399:intron | 30.0% |
| !! | ACCATTTTTGATGACATATG+AGG | + | chr8.3:25961017-25961036 | MS.gene87399:CDS | 30.0% |
| !! | CATTTTTGATGACATATGAG+GGG | + | chr8.3:25961019-25961038 | MS.gene87399:CDS | 30.0% |
| !! | CCATTTTTGATGACATATGA+GGG | + | chr8.3:25961018-25961037 | MS.gene87399:CDS | 30.0% |
| !!! | TGCCATTTTTCATAAAGAAC+GGG | + | chr8.3:25960801-25960820 | MS.gene87399:intron | 30.0% |
| AAGATTGTTGATCACTACAG+CGG | + | chr8.3:25961455-25961474 | MS.gene87399:CDS | 35.0% | |
| AGAAAGAACCAATTGCACTT+CGG | + | chr8.3:25960974-25960993 | MS.gene87399:CDS | 35.0% | |
| AGATGAATATGTGGAAGCAA+TGG | + | chr8.3:25960656-25960675 | MS.gene87399:CDS | 35.0% | |
| CCCTCATATGTCATCAAAAA+TGG | - | chr8.3:25961021-25961040 | None:intergenic | 35.0% | |
| GGTTCTTTCTCATCAATATC+TGG | - | chr8.3:25960964-25960983 | None:intergenic | 35.0% | |
| TCCACATATTCATCTTGCAA+CGG | - | chr8.3:25960651-25960670 | None:intergenic | 35.0% | |
| TGAATTTGAGCCAGAATATG+CGG | + | chr8.3:25960924-25960943 | MS.gene87399:CDS | 35.0% | |
| ! | ATAAAGAACGGGAACGTTTT+AGG | + | chr8.3:25960812-25960831 | MS.gene87399:intron | 35.0% |
| ! | GCATAACTTGCAATGGTTTA+GGG | + | chr8.3:25961126-25961145 | MS.gene87399:intron | 35.0% |
| ! | TCTCAATGTACTTGTTACAC+AGG | - | chr8.3:25961251-25961270 | None:intergenic | 35.0% |
| ACCGTTGCAAGATGAATATG+TGG | + | chr8.3:25960647-25960666 | MS.gene87399:CDS | 40.0% | |
| ATATTCATCTTGCAACGGTG+AGG | - | chr8.3:25960646-25960665 | None:intergenic | 40.0% | |
| CTCTTCAATTACTTCCTGGT+AGG | - | chr8.3:25961408-25961427 | None:intergenic | 40.0% | |
| GAAAGAACCAATTGCACTTC+GGG | + | chr8.3:25960975-25960994 | MS.gene87399:CDS | 40.0% | |
| GATTCGAAGTCAAGAAGAGT+GGG | + | chr8.3:25961041-25961060 | MS.gene87399:CDS | 40.0% | |
| GCAAAGTTTAACCTTGATGC+AGG | - | chr8.3:25960784-25960803 | None:intergenic | 40.0% | |
| TATTCATCTTGCAACGGTGA+GGG | - | chr8.3:25960645-25960664 | None:intergenic | 40.0% | |
| TCCTCAGTCATGGATAAGAT+TGG | - | chr8.3:25961085-25961104 | None:intergenic | 40.0% | |
| TCGGATTACTGAAGCATATG+AGG | + | chr8.3:25960599-25960618 | MS.gene87399:CDS | 40.0% | |
| TTCTAACTCGATATCCTACC+AGG | + | chr8.3:25961391-25961410 | MS.gene87399:intron | 40.0% | |
| TTCTTTGCAGTTACCCTATC+CGG | - | chr8.3:25961482-25961501 | None:intergenic | 40.0% | |
| TTTCTAGGTTACCTGCATCA+AGG | + | chr8.3:25960770-25960789 | MS.gene87399:intron | 40.0% | |
| ! | GGCATAACTTGCAATGGTTT+AGG | + | chr8.3:25961125-25961144 | MS.gene87399:intron | 40.0% |
| ! | TGTATTTTATGGGTGCTAGC+TGG | + | chr8.3:25961306-25961325 | MS.gene87399:intron | 40.0% |
| ACGGTTAGTGAACTCCTTCA+CGG | - | chr8.3:25961561-25961580 | None:intergenic | 45.0% | |
| ATTTGAGCCAGAATATGCGG+TGG | + | chr8.3:25960927-25960946 | MS.gene87399:CDS | 45.0% | |
| CCTGAAGAGAGATAACAGCA+CGG | - | chr8.3:25961580-25961599 | None:intergenic | 45.0% | |
| CTCATTATGAGTGTGTCTGC+TGG | + | chr8.3:25961671-25961690 | MS.gene87399:CDS | 45.0% | |
| GCCAATCTTATCCATGACTG+AGG | + | chr8.3:25961081-25961100 | MS.gene87399:intron | 45.0% | |
| GGATTCGAAGTCAAGAAGAG+TGG | + | chr8.3:25961040-25961059 | MS.gene87399:CDS | 45.0% | |
| TCAAACTCCACCGCATATTC+TGG | - | chr8.3:25960937-25960956 | None:intergenic | 45.0% | |
| AAGTGCACCATCTTCCGTGA+AGG | + | chr8.3:25961544-25961563 | MS.gene87399:CDS | 50.0% | |
| ACGGAAGATGGTGCACTTGT+AGG | - | chr8.3:25961542-25961561 | None:intergenic | 50.0% | |
| AGTGCATCCCGAAGTGCAAT+TGG | - | chr8.3:25960985-25961004 | None:intergenic | 50.0% | |
| GTGAACTCCTTCACGGAAGA+TGG | - | chr8.3:25961554-25961573 | None:intergenic | 50.0% | |
| TCGAAGTCAAGAAGAGTGGG+AGG | + | chr8.3:25961044-25961063 | MS.gene87399:CDS | 50.0% | |
| TGGGCAGTAATCCTCAGTCA+TGG | - | chr8.3:25961095-25961114 | None:intergenic | 50.0% | |
| TGTTGATCACTACAGCGGAC+CGG | + | chr8.3:25961460-25961479 | MS.gene87399:CDS | 50.0% | |
| ! | CCGTGCTGTTATCTCTCTTC+AGG | + | chr8.3:25961577-25961596 | MS.gene87399:CDS | 50.0% |
| ATCACTACAGCGGACCGGAT+AGG | + | chr8.3:25961465-25961484 | MS.gene87399:CDS | 55.0% | |
| TCACTACAGCGGACCGGATA+GGG | + | chr8.3:25961466-25961485 | MS.gene87399:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 25960594 | 25961732 | 25960594 | ID=MS.gene87399 |
| chr8.3 | mRNA | 25960594 | 25961732 | 25960594 | ID=MS.gene87399.t1;Parent=MS.gene87399 |
| chr8.3 | exon | 25960594 | 25960692 | 25960594 | ID=MS.gene87399.t1.exon1;Parent=MS.gene87399.t1 |
| chr8.3 | CDS | 25960594 | 25960692 | 25960594 | ID=cds.MS.gene87399.t1;Parent=MS.gene87399.t1 |
| chr8.3 | exon | 25960922 | 25961065 | 25960922 | ID=MS.gene87399.t1.exon2;Parent=MS.gene87399.t1 |
| chr8.3 | CDS | 25960922 | 25961065 | 25960922 | ID=cds.MS.gene87399.t1;Parent=MS.gene87399.t1 |
| chr8.3 | exon | 25961413 | 25961598 | 25961413 | ID=MS.gene87399.t1.exon3;Parent=MS.gene87399.t1 |
| chr8.3 | CDS | 25961413 | 25961598 | 25961413 | ID=cds.MS.gene87399.t1;Parent=MS.gene87399.t1 |
| chr8.3 | exon | 25961661 | 25961732 | 25961661 | ID=MS.gene87399.t1.exon4;Parent=MS.gene87399.t1 |
| chr8.3 | CDS | 25961661 | 25961732 | 25961661 | ID=cds.MS.gene87399.t1;Parent=MS.gene87399.t1 |
| Gene Sequence |
| Protein sequence |
