Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87752.t1 | XP_024626508.1 | 97 | 100 | 3 | 0 | 23 | 122 | 1 | 100 | 4.70E-44 | 187.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87752.t1 | G7KX21 | 97.0 | 100 | 3 | 0 | 23 | 122 | 6 | 105 | 3.4e-44 | 187.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene87752 | 0.8595 | 3.96E-63 | -1.69E-46 |
| MS.gene049369 | MS.gene87752 | 0.83721 | 5.94E-57 | -1.69E-46 |
| MS.gene050499 | MS.gene87752 | 0.801917 | 7.18E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene87752 | 0.826098 | 3.27E-54 | -1.69E-46 |
| MS.gene051360 | MS.gene87752 | 0.823068 | 1.69E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene87752 | 0.821107 | 4.81E-53 | -1.69E-46 |
| MS.gene052414 | MS.gene87752 | 0.833747 | 4.46E-56 | -1.69E-46 |
| MS.gene052484 | MS.gene87752 | 0.80323 | 3.85E-49 | -1.69E-46 |
| MS.gene052485 | MS.gene87752 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene87752 | 0.812338 | 4.46E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene87752 | 0.824309 | 8.66E-54 | -1.69E-46 |
| MS.gene053758 | MS.gene87752 | 0.831165 | 1.95E-55 | -1.69E-46 |
| MS.gene053912 | MS.gene87752 | 0.847475 | 1.13E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene87752 | 0.814534 | 1.47E-51 | -1.69E-46 |
| MS.gene056422 | MS.gene87752 | 0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene87752 | 0.823653 | 1.23E-53 | -1.69E-46 |
| MS.gene057076 | MS.gene87752 | 0.801219 | 9.98E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene87752 | 0.856386 | 3.33E-62 | -1.69E-46 |
| MS.gene057449 | MS.gene87752 | 0.801631 | 8.22E-49 | -1.69E-46 |
| MS.gene057537 | MS.gene87752 | 0.82631 | 2.91E-54 | -1.69E-46 |
| MS.gene057734 | MS.gene87752 | 0.826871 | 2.14E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene87752 | 0.824302 | 8.69E-54 | -1.69E-46 |
| MS.gene057823 | MS.gene87752 | 0.819426 | 1.17E-52 | -1.69E-46 |
| MS.gene058120 | MS.gene87752 | 0.826137 | 3.20E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene87752 | 0.843169 | 1.65E-58 | -1.69E-46 |
| MS.gene058948 | MS.gene87752 | 0.815156 | 1.07E-51 | -1.69E-46 |
| MS.gene059366 | MS.gene87752 | 0.848485 | 5.95E-60 | -1.69E-46 |
| MS.gene059368 | MS.gene87752 | 0.813407 | 2.60E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene87752 | 0.802445 | 5.59E-49 | -1.69E-46 |
| MS.gene059455 | MS.gene87752 | 0.808538 | 2.95E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene87752 | 0.828248 | 9.99E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene87752 | 0.805794 | 1.12E-49 | -1.69E-46 |
| MS.gene059711 | MS.gene87752 | 0.817659 | 2.94E-52 | -1.69E-46 |
| MS.gene059807 | MS.gene87752 | 0.814043 | 1.88E-51 | -1.69E-46 |
| MS.gene060372 | MS.gene87752 | 0.812931 | 3.31E-51 | -1.69E-46 |
| MS.gene060818 | MS.gene87752 | 0.805307 | 1.42E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene87752 | 0.855553 | 5.84E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87752.t1 | MTR_7g072470 | 94.175 | 103 | 6 | 0 | 23 | 125 | 6 | 108 | 2.20e-67 | 199 |
| MS.gene87752.t1 | MTR_5g083810 | 57.258 | 124 | 49 | 1 | 1 | 120 | 25 | 148 | 4.54e-43 | 139 |
| MS.gene87752.t1 | MTR_3g466150 | 48.000 | 125 | 61 | 1 | 1 | 121 | 1 | 125 | 1.65e-31 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87752.t1 | AT2G40435 | 57.031 | 128 | 50 | 2 | 1 | 123 | 1 | 128 | 3.30e-43 | 138 |
| MS.gene87752.t1 | AT2G40435 | 57.600 | 125 | 48 | 2 | 1 | 120 | 1 | 125 | 4.88e-43 | 138 |
| MS.gene87752.t1 | AT2G40435 | 57.600 | 125 | 48 | 2 | 1 | 120 | 1 | 125 | 1.33e-42 | 137 |
| MS.gene87752.t1 | AT3G56220 | 53.968 | 126 | 53 | 1 | 1 | 121 | 1 | 126 | 1.60e-40 | 132 |
| MS.gene87752.t1 | AT3G56220 | 54.400 | 125 | 52 | 1 | 1 | 120 | 1 | 125 | 4.30e-40 | 131 |
Find 22 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAACTCTTCTATGTATT+TGG | 0.128186 | 7.1:+29723405 | None:intergenic |
| TTTCATGATTAATGTGCTTT+TGG | 0.236031 | 7.1:-29723102 | MS.gene87752:CDS |
| TGGTATGCTGGTATCAATAC+TGG | 0.411863 | 7.1:-29723066 | MS.gene87752:CDS |
| CAACTCTTCTATGTATTTGG+AGG | 0.422808 | 7.1:+29723408 | None:intergenic |
| GAGTGTGAAAACCCTAGAAA+AGG | 0.453059 | 7.1:-29723126 | MS.gene87752:CDS |
| GAGGGAATGAACTCTGAGTT+AGG | 0.454266 | 7.1:-29723373 | MS.gene87752:CDS |
| CTCCTCCAACAGCTTCTAGC+TGG | 0.462141 | 7.1:+29722982 | None:intergenic |
| AGTATTGATACCAGCATACC+AGG | 0.467026 | 7.1:+29723068 | None:intergenic |
| TGAGTCCTCAACTACTCAAA+TGG | 0.469573 | 7.1:-29723344 | MS.gene87752:CDS |
| TTGTGACATCTCGAAGTTGT+TGG | 0.472854 | 7.1:+29723797 | None:intergenic |
| CTCATCCATTTGAGTAGTTG+AGG | 0.479517 | 7.1:+29723339 | None:intergenic |
| AAAGAATAGCCCTGGTATGC+TGG | 0.482593 | 7.1:-29723078 | MS.gene87752:CDS |
| GACTTGATGTACTTGATGCT+AGG | 0.505174 | 7.1:-29723025 | MS.gene87752:CDS |
| ACTTGATGTACTTGATGCTA+GGG | 0.510074 | 7.1:-29723024 | MS.gene87752:CDS |
| GGAGGCGTCCACAATAATTG+AGG | 0.529677 | 7.1:+29723426 | None:intergenic |
| ACTTTCCAGCTAGAAGCTGT+TGG | 0.533098 | 7.1:-29722987 | MS.gene87752:CDS |
| TTCCAGCTAGAAGCTGTTGG+AGG | 0.538408 | 7.1:-29722984 | MS.gene87752:CDS |
| TCAAATGGATGAGCAACCTA+TGG | 0.542120 | 7.1:-29723329 | MS.gene87752:intron |
| AGTGTGAAAACCCTAGAAAA+GGG | 0.544492 | 7.1:-29723125 | MS.gene87752:CDS |
| GAACAAAGCCTCAATTATTG+TGG | 0.549027 | 7.1:-29723434 | MS.gene87752:CDS |
| CTCAAGAGATCAAAGAAGAG+TGG | 0.631483 | 7.1:-29723824 | MS.gene87752:CDS |
| GTATTGATACCAGCATACCA+GGG | 0.698292 | 7.1:+29723069 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACATAAAAAAAAAAGTTT+AGG | - | chr7.1:29723106-29723125 | MS.gene87752:CDS | 10.0% |
| !! | TACATAAAAAAAAAAGTTTA+GGG | - | chr7.1:29723107-29723126 | MS.gene87752:CDS | 10.0% |
| !!! | GTATTTTATTTTATTTTTGT+AGG | - | chr7.1:29723647-29723666 | MS.gene87752:intron | 10.0% |
| !! | TCCTTTAATTAAATTCAAAT+GGG | - | chr7.1:29723328-29723347 | MS.gene87752:intron | 15.0% |
| !! | ACCCATTTGAATTTAATTAA+AGG | + | chr7.1:29723332-29723351 | None:intergenic | 20.0% |
| !! | ATATAAAAATAGAGTACAAG+AGG | + | chr7.1:29723542-29723561 | None:intergenic | 20.0% |
| !! | ATTAAATTCAAATGGGTAAT+TGG | - | chr7.1:29723335-29723354 | MS.gene87752:CDS | 20.0% |
| !! | ATTATCTAGTATATTCACTT+TGG | - | chr7.1:29723207-29723226 | MS.gene87752:intron | 20.0% |
| !! | CATTTGAATTTAATTAAAGG+AGG | + | chr7.1:29723329-29723348 | None:intergenic | 20.0% |
| !! | CTCCTTTAATTAAATTCAAA+TGG | - | chr7.1:29723327-29723346 | MS.gene87752:intron | 20.0% |
| !! | TAGTAGAAAATATTGAACTA+TGG | - | chr7.1:29723302-29723321 | MS.gene87752:intron | 20.0% |
| !!! | CTCTTCTTTTATATATAACT+TGG | - | chr7.1:29723512-29723531 | MS.gene87752:intron | 20.0% |
| ! | TAATAACATTGTGTCTTCAT+TGG | + | chr7.1:29723250-29723269 | None:intergenic | 25.0% |
| !! | TTTCATGATTAATGTGCTTT+TGG | - | chr7.1:29723695-29723714 | MS.gene87752:intron | 25.0% |
| !!! | TTTATGTGTGAGTTTTTTTG+TGG | - | chr7.1:29723073-29723092 | MS.gene87752:CDS | 25.0% |
| AAATATTGAGCTCTTCGTTT+AGG | + | chr7.1:29723172-29723191 | None:intergenic | 30.0% | |
| ATCTAAATACGCAAAAGATG+TGG | + | chr7.1:29723608-29723627 | None:intergenic | 30.0% | |
| CTTCAACTCTTCTATGTATT+TGG | + | chr7.1:29723395-29723414 | None:intergenic | 30.0% | |
| ! | AATCATGAAACCCTTTTCTA+GGG | + | chr7.1:29723685-29723704 | None:intergenic | 30.0% |
| ! | TAATCATGAAACCCTTTTCT+AGG | + | chr7.1:29723686-29723705 | None:intergenic | 30.0% |
| ACTTGATGTACTTGATGCTA+GGG | - | chr7.1:29723773-29723792 | MS.gene87752:intron | 35.0% | |
| AGAAGAGTTGAAGCAAAAAG+TGG | - | chr7.1:29723402-29723421 | MS.gene87752:CDS | 35.0% | |
| AGTGTGAAAACCCTAGAAAA+GGG | - | chr7.1:29723672-29723691 | MS.gene87752:intron | 35.0% | |
| ATATTCCAACCCAATAAAGG+AGG | - | chr7.1:29723130-29723149 | MS.gene87752:CDS | 35.0% | |
| CAACTCTTCTATGTATTTGG+AGG | + | chr7.1:29723392-29723411 | None:intergenic | 35.0% | |
| GAACAAAGCCTCAATTATTG+TGG | - | chr7.1:29723363-29723382 | MS.gene87752:CDS | 35.0% | |
| ! | GAGAAATAGAGTACTACCAT+AGG | + | chr7.1:29723487-29723506 | None:intergenic | 35.0% |
| ! | TCTTCGTTTAGGTTATATGC+TGG | + | chr7.1:29723161-29723180 | None:intergenic | 35.0% |
| ACAAGCTCAACTGCTGTAAT+TGG | - | chr7.1:29723019-29723038 | MS.gene87752:CDS | 40.0% | |
| AGAGTTGAAGCAAAAAGTGG+AGG | - | chr7.1:29723405-29723424 | MS.gene87752:CDS | 40.0% | |
| CTCAAGAGATCAAAGAAGAG+TGG | - | chr7.1:29722973-29722992 | MS.gene87752:CDS | 40.0% | |
| CTCATCCATTTGAGTAGTTG+AGG | + | chr7.1:29723461-29723480 | None:intergenic | 40.0% | |
| GACTTGATGTACTTGATGCT+AGG | - | chr7.1:29723772-29723791 | MS.gene87752:intron | 40.0% | |
| GAGTGTGAAAACCCTAGAAA+AGG | - | chr7.1:29723671-29723690 | MS.gene87752:intron | 40.0% | |
| GAGTTGAAGCAAAAAGTGGA+GGG | - | chr7.1:29723406-29723425 | MS.gene87752:CDS | 40.0% | |
| GGGATATTCCAACCCAATAA+AGG | - | chr7.1:29723127-29723146 | MS.gene87752:CDS | 40.0% | |
| TCAAATGGATGAGCAACCTA+TGG | - | chr7.1:29723468-29723487 | MS.gene87752:intron | 40.0% | |
| TGAGTCCTCAACTACTCAAA+TGG | - | chr7.1:29723453-29723472 | MS.gene87752:intron | 40.0% | |
| TGGTATGCTGGTATCAATAC+TGG | - | chr7.1:29723731-29723750 | MS.gene87752:intron | 40.0% | |
| TTGTGACATCTCGAAGTTGT+TGG | + | chr7.1:29723003-29723022 | None:intergenic | 40.0% | |
| ! | GTATTGATACCAGCATACCA+GGG | + | chr7.1:29723731-29723750 | None:intergenic | 40.0% |
| !! | ACTGGAAGCTTTTGAAGATC+TGG | - | chr7.1:29723749-29723768 | MS.gene87752:intron | 40.0% |
| !! | AGTATTGATACCAGCATACC+AGG | + | chr7.1:29723732-29723751 | None:intergenic | 40.0% |
| !! | CTGGAAGCTTTTGAAGATCT+GGG | - | chr7.1:29723750-29723769 | MS.gene87752:intron | 40.0% |
| !! | CTTTTGGAAAAGAATAGCCC+TGG | - | chr7.1:29723711-29723730 | MS.gene87752:intron | 40.0% |
| AAAGAATAGCCCTGGTATGC+TGG | - | chr7.1:29723719-29723738 | MS.gene87752:intron | 45.0% | |
| ACTTTCCAGCTAGAAGCTGT+TGG | - | chr7.1:29723810-29723829 | MS.gene87752:CDS | 45.0% | |
| CTGGCTTCTCCTCCTTTATT+GGG | + | chr7.1:29723142-29723161 | None:intergenic | 45.0% | |
| CTTCTCCTCCTTTATTGGGT+TGG | + | chr7.1:29723138-29723157 | None:intergenic | 45.0% | |
| GAGGGAATGAACTCTGAGTT+AGG | - | chr7.1:29723424-29723443 | MS.gene87752:CDS | 45.0% | |
| TTCCAGCTAGAAGCTGTTGG+AGG | - | chr7.1:29723813-29723832 | MS.gene87752:CDS | 50.0% | |
| ! | GCTGGCTTCTCCTCCTTTAT+TGG | + | chr7.1:29723143-29723162 | None:intergenic | 50.0% |
| !! | GGAGGCGTCCACAATAATTG+AGG | + | chr7.1:29723374-29723393 | None:intergenic | 50.0% |
| CTCCTCCAACAGCTTCTAGC+TGG | + | chr7.1:29723818-29723837 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 29722968 | 29723851 | 29722968 | ID=MS.gene87752 |
| chr7.1 | mRNA | 29722968 | 29723851 | 29722968 | ID=MS.gene87752.t1;Parent=MS.gene87752 |
| chr7.1 | exon | 29723786 | 29723851 | 29723786 | ID=MS.gene87752.t1.exon1;Parent=MS.gene87752.t1 |
| chr7.1 | CDS | 29723786 | 29723851 | 29723786 | ID=cds.MS.gene87752.t1;Parent=MS.gene87752.t1 |
| chr7.1 | exon | 29723330 | 29723458 | 29723330 | ID=MS.gene87752.t1.exon2;Parent=MS.gene87752.t1 |
| chr7.1 | CDS | 29723330 | 29723458 | 29723330 | ID=cds.MS.gene87752.t1;Parent=MS.gene87752.t1 |
| chr7.1 | exon | 29722968 | 29723150 | 29722968 | ID=MS.gene87752.t1.exon3;Parent=MS.gene87752.t1 |
| chr7.1 | CDS | 29722968 | 29723150 | 29722968 | ID=cds.MS.gene87752.t1;Parent=MS.gene87752.t1 |
| Gene Sequence |
| Protein sequence |
