Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87804.t1 | RHN79299.1 | 40.7 | 221 | 81 | 6 | 1 | 186 | 1 | 206 | 7.00E-20 | 107.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87804.t1 | A0A396JLZ9 | 40.7 | 221 | 81 | 6 | 1 | 186 | 1 | 206 | 5.0e-20 | 107.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene87804 | 0.80541 | 1.35E-49 | -1.69E-46 |
| MS.gene049563 | MS.gene87804 | 0.807747 | 4.34E-50 | -1.69E-46 |
| MS.gene050139 | MS.gene87804 | 0.815237 | 1.02E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene87804 | -0.802302 | 5.98E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene87804 | 0.813426 | 2.58E-51 | -1.69E-46 |
| MS.gene051626 | MS.gene87804 | 0.83941 | 1.61E-57 | -1.69E-46 |
| MS.gene051789 | MS.gene87804 | 0.813301 | 2.74E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene87804 | 0.800755 | 1.24E-48 | -1.69E-46 |
| MS.gene052778 | MS.gene87804 | 0.802548 | 5.32E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene87804 | 0.839797 | 1.28E-57 | -1.69E-46 |
| MS.gene053184 | MS.gene87804 | 0.806163 | 9.40E-50 | -1.69E-46 |
| MS.gene054184 | MS.gene87804 | 0.836758 | 7.76E-57 | -1.69E-46 |
| MS.gene054185 | MS.gene87804 | 0.842144 | 3.09E-58 | -1.69E-46 |
| MS.gene054596 | MS.gene87804 | 0.822071 | 2.88E-53 | -1.69E-46 |
| MS.gene054942 | MS.gene87804 | 0.809667 | 1.69E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene87804 | 0.808337 | 3.25E-50 | -1.69E-46 |
| MS.gene057395 | MS.gene87804 | 0.807605 | 4.66E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene87804 | 0.839933 | 1.18E-57 | -1.69E-46 |
| MS.gene059006 | MS.gene87804 | 0.822254 | 2.61E-53 | -1.69E-46 |
| MS.gene059124 | MS.gene87804 | 0.813347 | 2.68E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87804.t1 | MTR_1g054385 | 45.789 | 190 | 93 | 1 | 6 | 185 | 61 | 250 | 6.67e-46 | 151 |
| MS.gene87804.t1 | MTR_1g054380 | 41.176 | 204 | 70 | 6 | 1 | 169 | 1 | 189 | 5.87e-33 | 117 |
| MS.gene87804.t1 | MTR_5g048290 | 33.333 | 201 | 96 | 5 | 21 | 186 | 85 | 282 | 7.29e-18 | 79.3 |
| MS.gene87804.t1 | MTR_1g054370 | 29.902 | 204 | 102 | 5 | 21 | 186 | 90 | 290 | 4.30e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTTGTTCAAGGTTTA+AGG | 0.133169 | 1.2:-41753909 | None:intergenic |
| CTTGTAAACAATTCCTTCTT+TGG | 0.221022 | 1.2:-41753767 | None:intergenic |
| TCTGAATCTGAGAATAAAAT+AGG | 0.235016 | 1.2:+41753637 | MS.gene87804:CDS |
| CTTCGAAAAGAGTGGATTAA+TGG | 0.276341 | 1.2:+41753690 | MS.gene87804:CDS |
| GGAGCAAAAGGTTTGTGATT+TGG | 0.304158 | 1.2:+41753561 | MS.gene87804:CDS |
| CTGAATCTGAGAATAAAATA+GGG | 0.365988 | 1.2:+41753638 | MS.gene87804:CDS |
| CTTGTTCAAGGTTTAAGGTT+AGG | 0.370602 | 1.2:-41753904 | None:intergenic |
| GCAAGCAACACCCGTTCAAT+TGG | 0.411385 | 1.2:+41753519 | MS.gene87804:CDS |
| CGCTAAAATTGAAGAATTGA+AGG | 0.412911 | 1.2:+41753816 | MS.gene87804:CDS |
| TGCTCGTTGTATGGAGCAAA+AGG | 0.418843 | 1.2:+41753549 | MS.gene87804:CDS |
| GGAAAATTGCACACCAAAGA+AGG | 0.418990 | 1.2:+41753754 | MS.gene87804:CDS |
| ATGGCTACTCTAGATCAATC+TGG | 0.423439 | 1.2:+41753422 | MS.gene87804:CDS |
| GGTCTCGATTGCTCGTTGTA+TGG | 0.434380 | 1.2:+41753540 | MS.gene87804:CDS |
| AGAAGGAATTGTTTACAAGT+TGG | 0.457133 | 1.2:+41753771 | MS.gene87804:CDS |
| GTGATTTGGAAATGAGCTGC+AGG | 0.481810 | 1.2:+41753575 | MS.gene87804:CDS |
| AGGAATTGTTTACAAGTTGG+AGG | 0.487776 | 1.2:+41753774 | MS.gene87804:CDS |
| AAAGGATCATGAGGCATATG+AGG | 0.490646 | 1.2:+41753465 | MS.gene87804:CDS |
| CTAGAGTAGCCATTCTCAGC+TGG | 0.505923 | 1.2:-41753412 | None:intergenic |
| GAGTGGATTAATGGAAAGAA+CGG | 0.524492 | 1.2:+41753699 | MS.gene87804:CDS |
| TCATGAGGCATATGAGGAGT+GGG | 0.527499 | 1.2:+41753471 | MS.gene87804:CDS |
| GCAATCGAGACCAATTGAAC+GGG | 0.527802 | 1.2:-41753529 | None:intergenic |
| AAGACTACACCAGCTGAGAA+TGG | 0.552919 | 1.2:+41753403 | MS.gene87804:CDS |
| GCACAATGTTGGCTTTAATG+AGG | 0.553089 | 1.2:+41753855 | MS.gene87804:CDS |
| TGGCTACTCTAGATCAATCT+GGG | 0.553714 | 1.2:+41753423 | MS.gene87804:CDS |
| GAGTTAATCTTCGAAAAGAG+TGG | 0.570774 | 1.2:+41753682 | MS.gene87804:CDS |
| ATCATGAGGCATATGAGGAG+TGG | 0.581486 | 1.2:+41753470 | MS.gene87804:CDS |
| AGAATGGATCCCAAGAGATG+TGG | 0.586826 | 1.2:+41753358 | None:intergenic |
| ATTGCAGTGCAGCACAATGT+TGG | 0.599393 | 1.2:+41753844 | MS.gene87804:CDS |
| CGGGTGTTGCTTGCAAAACA+CGG | 0.601763 | 1.2:-41753510 | None:intergenic |
| TATCACAGAAAAGGATCATG+AGG | 0.608121 | 1.2:+41753456 | MS.gene87804:CDS |
| TGAATCTGAGAATAAAATAG+GGG | 0.621767 | 1.2:+41753639 | MS.gene87804:CDS |
| AGCAATCGAGACCAATTGAA+CGG | 0.624783 | 1.2:-41753530 | None:intergenic |
| TGATTTGGAAATGAGCTGCA+GGG | 0.643574 | 1.2:+41753576 | MS.gene87804:CDS |
| CATGAGGCATATGAGGAGTG+GGG | 0.692602 | 1.2:+41753472 | MS.gene87804:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGAATCTGAGAATAAAATA+GGG | + | chr1.2:41753638-41753657 | MS.gene87804:CDS | 25.0% |
| ! | TCTGAATCTGAGAATAAAAT+AGG | + | chr1.2:41753637-41753656 | MS.gene87804:CDS | 25.0% |
| ! | TGAATCTGAGAATAAAATAG+GGG | + | chr1.2:41753639-41753658 | MS.gene87804:CDS | 25.0% |
| !! | AATGAAAACTCCAAGATTTT+AGG | + | chr1.2:41753733-41753752 | MS.gene87804:CDS | 25.0% |
| !! | TGACAATTTTATCACAGAAA+AGG | + | chr1.2:41753447-41753466 | MS.gene87804:CDS | 25.0% |
| AGAAGGAATTGTTTACAAGT+TGG | + | chr1.2:41753771-41753790 | MS.gene87804:CDS | 30.0% | |
| CGCTAAAATTGAAGAATTGA+AGG | + | chr1.2:41753816-41753835 | MS.gene87804:CDS | 30.0% | |
| CTTGTAAACAATTCCTTCTT+TGG | - | chr1.2:41753770-41753789 | None:intergenic | 30.0% | |
| ! | GTGCAATTTTCCTAAAATCT+TGG | - | chr1.2:41753746-41753765 | None:intergenic | 30.0% |
| GAGTTAATCTTCGAAAAGAG+TGG | + | chr1.2:41753682-41753701 | MS.gene87804:CDS | 35.0% | |
| TATCACAGAAAAGGATCATG+AGG | + | chr1.2:41753456-41753475 | MS.gene87804:CDS | 35.0% | |
| ! | AGGAATTGTTTACAAGTTGG+AGG | + | chr1.2:41753774-41753793 | MS.gene87804:CDS | 35.0% |
| !! | CTTCGAAAAGAGTGGATTAA+TGG | + | chr1.2:41753690-41753709 | MS.gene87804:CDS | 35.0% |
| !! | GAGTGGATTAATGGAAAGAA+CGG | + | chr1.2:41753699-41753718 | MS.gene87804:CDS | 35.0% |
| AAAGGATCATGAGGCATATG+AGG | + | chr1.2:41753465-41753484 | MS.gene87804:CDS | 40.0% | |
| AGCAATCGAGACCAATTGAA+CGG | - | chr1.2:41753533-41753552 | None:intergenic | 40.0% | |
| ATGGCTACTCTAGATCAATC+TGG | + | chr1.2:41753422-41753441 | MS.gene87804:CDS | 40.0% | |
| GGAAAATTGCACACCAAAGA+AGG | + | chr1.2:41753754-41753773 | MS.gene87804:CDS | 40.0% | |
| GGAGCAAAAGGTTTGTGATT+TGG | + | chr1.2:41753561-41753580 | MS.gene87804:CDS | 40.0% | |
| TGGCTACTCTAGATCAATCT+GGG | + | chr1.2:41753423-41753442 | MS.gene87804:CDS | 40.0% | |
| ! | CTACGTTTTCCACATCTCTT+GGG | - | chr1.2:41753370-41753389 | None:intergenic | 40.0% |
| ! | TGATTTGGAAATGAGCTGCA+GGG | + | chr1.2:41753576-41753595 | MS.gene87804:CDS | 40.0% |
| !! | GCACAATGTTGGCTTTAATG+AGG | + | chr1.2:41753855-41753874 | MS.gene87804:CDS | 40.0% |
| AAGACTACACCAGCTGAGAA+TGG | + | chr1.2:41753403-41753422 | MS.gene87804:CDS | 45.0% | |
| ATCATGAGGCATATGAGGAG+TGG | + | chr1.2:41753470-41753489 | MS.gene87804:CDS | 45.0% | |
| ATTGCAGTGCAGCACAATGT+TGG | + | chr1.2:41753844-41753863 | MS.gene87804:CDS | 45.0% | |
| GCAATCGAGACCAATTGAAC+GGG | - | chr1.2:41753532-41753551 | None:intergenic | 45.0% | |
| TCATGAGGCATATGAGGAGT+GGG | + | chr1.2:41753471-41753490 | MS.gene87804:CDS | 45.0% | |
| TGCTCGTTGTATGGAGCAAA+AGG | + | chr1.2:41753549-41753568 | MS.gene87804:CDS | 45.0% | |
| ! | GCTACGTTTTCCACATCTCT+TGG | - | chr1.2:41753371-41753390 | None:intergenic | 45.0% |
| ! | GTGATTTGGAAATGAGCTGC+AGG | + | chr1.2:41753575-41753594 | MS.gene87804:CDS | 45.0% |
| CATGAGGCATATGAGGAGTG+GGG | + | chr1.2:41753472-41753491 | MS.gene87804:CDS | 50.0% | |
| CTAGAGTAGCCATTCTCAGC+TGG | - | chr1.2:41753415-41753434 | None:intergenic | 50.0% | |
| GCAAGCAACACCCGTTCAAT+TGG | + | chr1.2:41753519-41753538 | MS.gene87804:CDS | 50.0% | |
| GGTCTCGATTGCTCGTTGTA+TGG | + | chr1.2:41753540-41753559 | MS.gene87804:CDS | 50.0% | |
| !! | CGGGTGTTGCTTGCAAAACA+CGG | - | chr1.2:41753513-41753532 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 41753361 | 41753921 | 41753361 | ID=MS.gene87804 |
| chr1.2 | mRNA | 41753361 | 41753921 | 41753361 | ID=MS.gene87804.t1;Parent=MS.gene87804 |
| chr1.2 | exon | 41753361 | 41753921 | 41753361 | ID=MS.gene87804.t1.exon1;Parent=MS.gene87804.t1 |
| chr1.2 | CDS | 41753361 | 41753921 | 41753361 | ID=cds.MS.gene87804.t1;Parent=MS.gene87804.t1 |
| Gene Sequence |
| Protein sequence |
