Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90075.t1 | XP_013453681.1 | 97.5 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 1.10E-47 | 199.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90075.t1 | A0A072UDK7 | 97.5 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 8.2e-48 | 199.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene90075 | 0.80333 | 3.67E-49 | -1.69E-46 |
| MS.gene050577 | MS.gene90075 | -0.803015 | 4.26E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene90075 | 0.802173 | 6.36E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene90075 | 0.819606 | 1.06E-52 | -1.69E-46 |
| MS.gene052211 | MS.gene90075 | 0.816718 | 4.79E-52 | -1.69E-46 |
| MS.gene052486 | MS.gene90075 | -0.802395 | 5.73E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene90075 | 0.822316 | 2.53E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene90075 | 0.809165 | 2.17E-50 | -1.69E-46 |
| MS.gene053421 | MS.gene90075 | 0.804608 | 1.99E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene90075 | -0.803626 | 3.19E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene90075 | 0.83976 | 1.31E-57 | -1.69E-46 |
| MS.gene056504 | MS.gene90075 | -0.804103 | 2.54E-49 | -1.69E-46 |
| MS.gene056590 | MS.gene90075 | 0.803489 | 3.40E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene90075 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene90075 | 0.824123 | 9.57E-54 | -1.69E-46 |
| MS.gene056856 | MS.gene90075 | 0.814846 | 1.25E-51 | -1.69E-46 |
| MS.gene057261 | MS.gene90075 | 0.801464 | 8.89E-49 | -1.69E-46 |
| MS.gene057768 | MS.gene90075 | 0.838613 | 2.59E-57 | -1.69E-46 |
| MS.gene058111 | MS.gene90075 | 0.815723 | 7.99E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene90075 | 0.808278 | 3.35E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene90075 | 0.811822 | 5.78E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene90075 | -0.81768 | 2.91E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene90075 | -0.800653 | 1.30E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90075.t1 | MTR_5g030310 | 99.167 | 120 | 1 | 0 | 1 | 120 | 1 | 120 | 8.87e-83 | 238 |
| MS.gene90075.t1 | MTR_5g430440 | 98.333 | 120 | 2 | 0 | 1 | 120 | 1 | 120 | 9.82e-82 | 235 |
| MS.gene90075.t1 | MTR_5g430430 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 8.19e-69 | 202 |
| MS.gene90075.t1 | MTR_5g030290 | 83.333 | 120 | 20 | 0 | 1 | 120 | 1 | 120 | 8.19e-69 | 202 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGGCCTTCAGTCTTGAAAA+TGG | 0.356723 | 5.2:-30838 | None:intergenic |
| TTATGCAGTGATGGTGGAGC+TGG | 0.397441 | 5.2:+30741 | MS.gene90075:intron |
| CTTGCTCTTGAGTGAAGCTC+TGG | 0.411535 | 5.2:-29872 | None:intergenic |
| TCACTGCTACTCTCAATAAC+TGG | 0.438274 | 5.2:-30768 | None:intergenic |
| CCCTTGCCAGAGTTTACACC+AGG | 0.459349 | 5.2:-30858 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.486701 | 5.2:+30106 | MS.gene90075:CDS |
| AGGACTGAAGGGCCTGATGC+TGG | 0.490624 | 5.2:+30154 | MS.gene90075:CDS |
| ACAACACATTGCCCAAGCCC+TGG | 0.525921 | 5.2:-30096 | None:intergenic |
| CCCTGGTGTAAACTCTGGCA+AGG | 0.541663 | 5.2:+30857 | MS.gene90075:CDS |
| TATGCAGTGATGGTGGAGCT+GGG | 0.557903 | 5.2:+30742 | MS.gene90075:intron |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.561546 | 5.2:+30804 | MS.gene90075:CDS |
| ATTGAGAGTAGCAGTGACAA+TGG | 0.563224 | 5.2:+30774 | MS.gene90075:CDS |
| CAGAGCTTCACTCAAGAGCA+AGG | 0.567749 | 5.2:+29873 | MS.gene90075:CDS |
| TTGCTCTTGAGTGAAGCTCT+GGG | 0.573531 | 5.2:-29871 | None:intergenic |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5.2:+30134 | MS.gene90075:CDS |
| GTGTTATAACTAGGACTGAA+GGG | 0.595455 | 5.2:+30143 | MS.gene90075:CDS |
| AAACTCTTATGCAGTGATGG+TGG | 0.602169 | 5.2:+30735 | MS.gene90075:intron |
| GAAGGCCCTGGTGTAAACTC+TGG | 0.603912 | 5.2:+30852 | MS.gene90075:CDS |
| TGTGTTATAACTAGGACTGA+AGG | 0.610701 | 5.2:+30142 | MS.gene90075:CDS |
| CCTGGTGTAAACTCTGGCAA+GGG | 0.620143 | 5.2:+30858 | MS.gene90075:CDS |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5.2:+30112 | MS.gene90075:CDS |
| AGAGCTTCACTCAAGAGCAA+GGG | 0.651871 | 5.2:+29874 | MS.gene90075:CDS |
| TCTGCAAGTGTGGTGAAGGG+TGG | 0.652623 | 5.2:+30808 | MS.gene90075:CDS |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5.2:+30116 | MS.gene90075:CDS |
| AGTGAAACATTCTGCAAGTG+TGG | 0.671634 | 5.2:+30798 | MS.gene90075:CDS |
| CATTCTGCAAGTGTGGTGAA+GGG | 0.681189 | 5.2:+30805 | MS.gene90075:CDS |
| CCTTGCCAGAGTTTACACCA+GGG | 0.773669 | 5.2:-30857 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTATTTGTTTGTAATA+TGG | + | chr5.2:30434-30453 | MS.gene90075:intron | 10.0% |
| !! | TAATATGAAATTGAGATTTA+AGG | - | chr5.2:30559-30578 | None:intergenic | 15.0% |
| !! | AAACTGTAAAGATGAAATAA+AGG | - | chr5.2:29931-29950 | None:intergenic | 20.0% |
| !! | AATTTATCGTTCAACTTATT+AGG | - | chr5.2:30519-30538 | None:intergenic | 20.0% |
| !! | GCAGTTAAAATAATAATAAG+AGG | - | chr5.2:30239-30258 | None:intergenic | 20.0% |
| !! | TAAAATAATAATAAGAGGAG+TGG | - | chr5.2:30234-30253 | None:intergenic | 20.0% |
| !! | TTATTAGGTAAAAGTTCAAT+AGG | - | chr5.2:30504-30523 | None:intergenic | 20.0% |
| ! | AAAGTGTGAAAGACTATATT+AGG | + | chr5.2:30365-30384 | MS.gene90075:intron | 25.0% |
| ! | ATTATGACAGGTATGAAAAA+TGG | - | chr5.2:30025-30044 | None:intergenic | 25.0% |
| ! | TGTAAAGATGAAATAAAGGA+TGG | - | chr5.2:29927-29946 | None:intergenic | 25.0% |
| CTAAAATGTCAAATACTGCT+TGG | - | chr5.2:30310-30329 | None:intergenic | 30.0% | |
| ! | AAAAATGGAGCGCATTATTT+TGG | - | chr5.2:30010-30029 | None:intergenic | 30.0% |
| ! | GCTTATTGGTTAATTATGAC+AGG | - | chr5.2:30037-30056 | None:intergenic | 30.0% |
| ! | TTAACCAATAAGCTTTGTGT+AGG | + | chr5.2:30044-30063 | MS.gene90075:intron | 30.0% |
| !! | GCAATCATTTTAACAATGGT+TGG | - | chr5.2:30461-30480 | None:intergenic | 30.0% |
| !! | TGGTGCAATCATTTTAACAA+TGG | - | chr5.2:30465-30484 | None:intergenic | 30.0% |
| ATCAAACTCTTATGCAGTGA+TGG | + | chr5.2:30732-30751 | MS.gene90075:intron | 35.0% | |
| CTTTCCTACACAAAGCTTAT+TGG | - | chr5.2:30051-30070 | None:intergenic | 35.0% | |
| GTGTTATAACTAGGACTGAA+GGG | + | chr5.2:30143-30162 | MS.gene90075:CDS | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | + | chr5.2:30134-30153 | MS.gene90075:CDS | 35.0% | |
| TAGGTAAGATCTTCATGTCA+TGG | - | chr5.2:30485-30504 | None:intergenic | 35.0% | |
| TGTGTTATAACTAGGACTGA+AGG | + | chr5.2:30142-30161 | MS.gene90075:CDS | 35.0% | |
| ! | CTGAGTTTTTATCTTGAGCT+GGG | - | chr5.2:30075-30094 | None:intergenic | 35.0% |
| ! | GGCAAAGTTTTTGCTAAATG+TGG | + | chr5.2:30175-30194 | MS.gene90075:CDS | 35.0% |
| !! | TGTTCCATTTTCAAGACTGA+AGG | + | chr5.2:30834-30853 | MS.gene90075:CDS | 35.0% |
| AAACTCTTATGCAGTGATGG+TGG | + | chr5.2:30735-30754 | MS.gene90075:intron | 40.0% | |
| AGTGAAACATTCTGCAAGTG+TGG | + | chr5.2:30798-30817 | MS.gene90075:CDS | 40.0% | |
| ATTGAGAGTAGCAGTGACAA+TGG | + | chr5.2:30774-30793 | MS.gene90075:CDS | 40.0% | |
| CAAGATAAAAACTCAGGCCA+GGG | + | chr5.2:30079-30098 | MS.gene90075:CDS | 40.0% | |
| CCAGCTCAAGATAAAAACTC+AGG | + | chr5.2:30073-30092 | MS.gene90075:CDS | 40.0% | |
| TCAAGATAAAAACTCAGGCC+AGG | + | chr5.2:30078-30097 | MS.gene90075:CDS | 40.0% | |
| TCACTGCTACTCTCAATAAC+TGG | - | chr5.2:30771-30790 | None:intergenic | 40.0% | |
| ! | CCTGAGTTTTTATCTTGAGC+TGG | - | chr5.2:30076-30095 | None:intergenic | 40.0% |
| ACATTCTGCAAGTGTGGTGA+AGG | + | chr5.2:30804-30823 | MS.gene90075:CDS | 45.0% | |
| AGAGCTTCACTCAAGAGCAA+GGG | + | chr5.2:29874-29893 | MS.gene90075:CDS | 45.0% | |
| AGGGCCTTCAGTCTTGAAAA+TGG | - | chr5.2:30841-30860 | None:intergenic | 45.0% | |
| CATTCTGCAAGTGTGGTGAA+GGG | + | chr5.2:30805-30824 | MS.gene90075:CDS | 45.0% | |
| GCAAAAACTTTGCCAGCATC+AGG | - | chr5.2:30169-30188 | None:intergenic | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | + | chr5.2:30112-30131 | MS.gene90075:CDS | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | + | chr5.2:30116-30135 | MS.gene90075:CDS | 45.0% | |
| TTGCTCTTGAGTGAAGCTCT+GGG | - | chr5.2:29874-29893 | None:intergenic | 45.0% | |
| !! | ATTTTCAAGACTGAAGGCCC+TGG | + | chr5.2:30840-30859 | MS.gene90075:CDS | 45.0% |
| AAAAACTCAGGCCAGGGCTT+GGG | + | chr5.2:30085-30104 | MS.gene90075:CDS | 50.0% | |
| CAGAGCTTCACTCAAGAGCA+AGG | + | chr5.2:29873-29892 | MS.gene90075:CDS | 50.0% | |
| CCTGGTGTAAACTCTGGCAA+GGG | + | chr5.2:30858-30877 | MS.gene90075:CDS | 50.0% | |
| CCTTGCCAGAGTTTACACCA+GGG | - | chr5.2:30860-30879 | None:intergenic | 50.0% | |
| CTTGCTCTTGAGTGAAGCTC+TGG | - | chr5.2:29875-29894 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | + | chr5.2:30106-30125 | MS.gene90075:CDS | 50.0% | |
| TAAAAACTCAGGCCAGGGCT+TGG | + | chr5.2:30084-30103 | MS.gene90075:CDS | 50.0% | |
| !! | TATGCAGTGATGGTGGAGCT+GGG | + | chr5.2:30742-30761 | MS.gene90075:intron | 50.0% |
| !! | TTATGCAGTGATGGTGGAGC+TGG | + | chr5.2:30741-30760 | MS.gene90075:intron | 50.0% |
| ACAACACATTGCCCAAGCCC+TGG | - | chr5.2:30099-30118 | None:intergenic | 55.0% | |
| CCCTGGTGTAAACTCTGGCA+AGG | + | chr5.2:30857-30876 | MS.gene90075:CDS | 55.0% | |
| CCCTTGCCAGAGTTTACACC+AGG | - | chr5.2:30861-30880 | None:intergenic | 55.0% | |
| GAAGGCCCTGGTGTAAACTC+TGG | + | chr5.2:30852-30871 | MS.gene90075:CDS | 55.0% | |
| TCTGCAAGTGTGGTGAAGGG+TGG | + | chr5.2:30808-30827 | MS.gene90075:CDS | 55.0% | |
| AGGACTGAAGGGCCTGATGC+TGG | + | chr5.2:30154-30173 | MS.gene90075:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 29864 | 30926 | 29864 | ID=MS.gene90075 |
| chr5.2 | mRNA | 29864 | 30926 | 29864 | ID=MS.gene90075.t1;Parent=MS.gene90075 |
| chr5.2 | exon | 29864 | 29895 | 29864 | ID=MS.gene90075.t1.exon1;Parent=MS.gene90075.t1 |
| chr5.2 | CDS | 29864 | 29895 | 29864 | ID=cds.MS.gene90075.t1;Parent=MS.gene90075.t1 |
| chr5.2 | exon | 30066 | 30218 | 30066 | ID=MS.gene90075.t1.exon2;Parent=MS.gene90075.t1 |
| chr5.2 | CDS | 30066 | 30218 | 30066 | ID=cds.MS.gene90075.t1;Parent=MS.gene90075.t1 |
| chr5.2 | exon | 30749 | 30926 | 30749 | ID=MS.gene90075.t1.exon3;Parent=MS.gene90075.t1 |
| chr5.2 | CDS | 30749 | 30926 | 30749 | ID=cds.MS.gene90075.t1;Parent=MS.gene90075.t1 |
| Gene Sequence |
| Protein sequence |
