Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92367.t1 | XP_013453298.1 | 98.8 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 3.30E-38 | 167.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92367.t1 | Q1G3M2 | 72.5 | 80 | 21 | 1 | 1 | 80 | 1 | 79 | 1.6e-25 | 116.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92367.t1 | A0A072UCK1 | 98.8 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 2.4e-38 | 167.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049255 | MS.gene92367 | 0.817171 | 3.79E-52 | -1.69E-46 |
| MS.gene050277 | MS.gene92367 | 0.853267 | 2.68E-61 | -1.69E-46 |
| MS.gene050752 | MS.gene92367 | 0.806763 | 7.02E-50 | -1.69E-46 |
| MS.gene050955 | MS.gene92367 | 0.800372 | 1.49E-48 | -1.69E-46 |
| MS.gene051241 | MS.gene92367 | 0.817875 | 2.63E-52 | -1.69E-46 |
| MS.gene051746 | MS.gene92367 | 0.807785 | 4.27E-50 | -1.69E-46 |
| MS.gene051748 | MS.gene92367 | 0.810995 | 8.73E-51 | -1.69E-46 |
| MS.gene051903 | MS.gene92367 | 0.817914 | 2.58E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene92367 | 0.827146 | 1.84E-54 | -1.69E-46 |
| MS.gene052947 | MS.gene92367 | 0.804051 | 2.60E-49 | -1.69E-46 |
| MS.gene053109 | MS.gene92367 | 0.805882 | 1.08E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene92367 | 0.804352 | 2.25E-49 | -1.69E-46 |
| MS.gene056631 | MS.gene92367 | 0.851517 | 8.43E-61 | -1.69E-46 |
| MS.gene057079 | MS.gene92367 | 0.801801 | 7.58E-49 | -1.69E-46 |
| MS.gene057234 | MS.gene92367 | 0.814676 | 1.36E-51 | -1.69E-46 |
| MS.gene057705 | MS.gene92367 | 0.826226 | 3.05E-54 | -1.69E-46 |
| MS.gene058238 | MS.gene92367 | 0.816345 | 5.80E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene92367 | 0.804017 | 2.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92367.t1 | MTR_5g004970 | 98.765 | 81 | 1 | 0 | 1 | 81 | 1 | 81 | 1.59e-55 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92367.t1 | AT3G19508 | 72.500 | 80 | 21 | 1 | 1 | 80 | 1 | 79 | 4.66e-36 | 117 |
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTCTATGGGTTCTTCATA+AGG | 0.314427 | 5.1:-226310 | MS.gene92367:intron |
| GTACCTACACTCATTCTCTA+TGG | 0.327232 | 5.1:-226323 | MS.gene92367:CDS |
| TAATAACCTCTTGAATCCTT+TGG | 0.385434 | 5.1:+226429 | None:intergenic |
| AGCTCAAGGTGATATGTTGC+GGG | 0.429923 | 5.1:-226176 | MS.gene92367:CDS |
| AGGTACGTTGAAAGAGATCA+TGG | 0.523995 | 5.1:+226340 | None:intergenic |
| AAAGAGATCATGGAGAGTAG+TGG | 0.552251 | 5.1:+226350 | None:intergenic |
| TACCTACACTCATTCTCTAT+GGG | 0.556578 | 5.1:-226322 | MS.gene92367:CDS |
| GTTAGATTAGATGGAGAAAG+CGG | 0.560882 | 5.1:-226493 | None:intergenic |
| AAGCTCAAGGTGATATGTTG+CGG | 0.562731 | 5.1:-226177 | MS.gene92367:CDS |
| ACTTGTGCGGCGTTTACCAA+AGG | 0.567162 | 5.1:-226445 | MS.gene92367:CDS |
| CGTTTACCAAAGGATTCAAG+AGG | 0.591068 | 5.1:-226435 | MS.gene92367:CDS |
| ATCTGCCGCTGATAAGCTCA+AGG | 0.615005 | 5.1:-226190 | MS.gene92367:CDS |
| TGGAGAGTAGTGGAATCAGA+AGG | 0.642946 | 5.1:+226360 | None:intergenic |
| AATCAGAAGGATCAACCTCA+CGG | 0.688438 | 5.1:+226373 | None:intergenic |
| CAGAAGTTCTGAGACTTGTG+CGG | 0.691962 | 5.1:-226458 | MS.gene92367:CDS |
| GAACTTCGTTAATTACCGTG+AGG | 0.698092 | 5.1:-226388 | MS.gene92367:CDS |
| TATCACCTTGAGCTTATCAG+CGG | 0.704627 | 5.1:+226185 | None:intergenic |
| AACCCATAGAGAATGAGTGT+AGG | 0.739379 | 5.1:+226320 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CACTCATTCATTTCTGTTTA+TGG | - | chr5.1:226376-226395 | MS.gene92367:CDS | 30.0% | |
| TAATAACCTCTTGAATCCTT+TGG | + | chr5.1:226230-226249 | None:intergenic | 30.0% | |
| TACCTACACTCATTCTCTAT+GGG | - | chr5.1:226334-226353 | MS.gene92367:CDS | 35.0% | |
| TTCTCTATGGGTTCTTCATA+AGG | - | chr5.1:226346-226365 | MS.gene92367:CDS | 35.0% | |
| AAAGAGATCATGGAGAGTAG+TGG | + | chr5.1:226309-226328 | None:intergenic | 40.0% | |
| AACCCATAGAGAATGAGTGT+AGG | + | chr5.1:226339-226358 | None:intergenic | 40.0% | |
| AAGCTCAAGGTGATATGTTG+CGG | - | chr5.1:226479-226498 | MS.gene92367:CDS | 40.0% | |
| AATCAGAAGGATCAACCTCA+CGG | + | chr5.1:226286-226305 | None:intergenic | 40.0% | |
| AGGTACGTTGAAAGAGATCA+TGG | + | chr5.1:226319-226338 | None:intergenic | 40.0% | |
| CGTTTACCAAAGGATTCAAG+AGG | - | chr5.1:226221-226240 | MS.gene92367:intron | 40.0% | |
| GAACTTCGTTAATTACCGTG+AGG | - | chr5.1:226268-226287 | MS.gene92367:intron | 40.0% | |
| GTACCTACACTCATTCTCTA+TGG | - | chr5.1:226333-226352 | MS.gene92367:CDS | 40.0% | |
| TATCACCTTGAGCTTATCAG+CGG | + | chr5.1:226474-226493 | None:intergenic | 40.0% | |
| TGCAATGCTACTGCAGTATT+CGG | - | chr5.1:226436-226455 | MS.gene92367:CDS | 40.0% | |
| AGCTCAAGGTGATATGTTGC+GGG | - | chr5.1:226480-226499 | MS.gene92367:CDS | 45.0% | |
| ! | TGGAGAGTAGTGGAATCAGA+AGG | + | chr5.1:226299-226318 | None:intergenic | 45.0% |
| !! | CAGAAGTTCTGAGACTTGTG+CGG | - | chr5.1:226198-226217 | MS.gene92367:CDS | 45.0% |
| ACTTGTGCGGCGTTTACCAA+AGG | - | chr5.1:226211-226230 | MS.gene92367:intron | 50.0% | |
| ATCTGCCGCTGATAAGCTCA+AGG | - | chr5.1:226466-226485 | MS.gene92367:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 226173 | 226505 | 226173 | ID=MS.gene92367 |
| chr5.1 | mRNA | 226173 | 226505 | 226173 | ID=MS.gene92367.t1;Parent=MS.gene92367 |
| chr5.1 | exon | 226311 | 226505 | 226311 | ID=MS.gene92367.t1.exon1;Parent=MS.gene92367.t1 |
| chr5.1 | CDS | 226311 | 226505 | 226311 | ID=cds.MS.gene92367.t1;Parent=MS.gene92367.t1 |
| chr5.1 | exon | 226173 | 226226 | 226173 | ID=MS.gene92367.t1.exon2;Parent=MS.gene92367.t1 |
| chr5.1 | CDS | 226173 | 226226 | 226173 | ID=cds.MS.gene92367.t1;Parent=MS.gene92367.t1 |
| Gene Sequence |
| Protein sequence |
