Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92389.t1 | RHN40223.1 | 95.6 | 91 | 3 | 1 | 1 | 90 | 54 | 144 | 1.00E-37 | 165.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92389.t1 | Q84MD2 | 45.1 | 91 | 39 | 4 | 1 | 90 | 3 | 83 | 4.6e-10 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92389.t1 | G7LEJ0 | 95.6 | 91 | 3 | 1 | 1 | 90 | 1 | 91 | 7.5e-38 | 165.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene92389 | 0.80819 | 3.50E-50 | -1.69E-46 |
| MS.gene050772 | MS.gene92389 | -0.801823 | 7.51E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene92389 | 0.816946 | 4.25E-52 | -1.69E-46 |
| MS.gene051366 | MS.gene92389 | 0.811306 | 7.48E-51 | -1.69E-46 |
| MS.gene051367 | MS.gene92389 | 0.80286 | 4.59E-49 | -1.69E-46 |
| MS.gene052211 | MS.gene92389 | 0.800361 | 1.49E-48 | -1.69E-46 |
| MS.gene05240 | MS.gene92389 | 0.804582 | 2.02E-49 | -1.69E-46 |
| MS.gene05244 | MS.gene92389 | 0.805496 | 1.30E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene92389 | 0.842777 | 2.10E-58 | -1.69E-46 |
| MS.gene054707 | MS.gene92389 | 0.805629 | 1.22E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene92389 | 0.823591 | 1.28E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene92389 | -0.802044 | 6.76E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene92389 | -0.806793 | 6.92E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene92389 | 0.829285 | 5.61E-55 | -1.69E-46 |
| MS.gene058099 | MS.gene92389 | 0.810663 | 1.03E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene92389 | 0.815118 | 1.09E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene92389 | -0.806006 | 1.01E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92389.t1 | MTR_8g037800 | 95.604 | 91 | 3 | 1 | 1 | 90 | 1 | 91 | 3.32e-49 | 150 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene92389.t1 | AT2G16385 | 45.055 | 91 | 39 | 4 | 1 | 90 | 3 | 83 | 5.38e-18 | 71.6 |
| MS.gene92389.t1 | AT4G34600 | 39.506 | 81 | 38 | 3 | 11 | 90 | 13 | 83 | 1.62e-12 | 57.8 |
Find 12 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCTTCCTCCTCATTTC+TGG | 0.032595 | 8.4:+60427551 | MS.gene92389:CDS |
| GCATCCATGAGAGGCTTCTT+AGG | 0.213059 | 8.4:+60428744 | MS.gene92389:CDS |
| AGGGCTAATACAAAAGATTA+TGG | 0.330875 | 8.4:+60428764 | MS.gene92389:CDS |
| CAATTTGGTATGAGCTTGAA+AGG | 0.467961 | 8.4:-60428821 | None:intergenic |
| CATCCATGAGAGGCTTCTTA+GGG | 0.497324 | 8.4:+60428745 | MS.gene92389:CDS |
| TAGCCCTAAGAAGCCTCTCA+TGG | 0.540665 | 8.4:-60428748 | None:intergenic |
| AGCAAAGAAGTCAATGAATG+AGG | 0.570640 | 8.4:+60428709 | MS.gene92389:CDS |
| AAGTAAGAAGCATCCATGAG+AGG | 0.614358 | 8.4:+60428735 | MS.gene92389:CDS |
| TCAAAAGTGAGCCAGAAATG+AGG | 0.623763 | 8.4:-60427562 | None:intergenic |
| TTTGGTATGAGCTTGAAAGG+AGG | 0.661865 | 8.4:-60428818 | None:intergenic |
| AAAGTGAGCCAGAAATGAGG+AGG | 0.663281 | 8.4:-60427559 | None:intergenic |
| AGATGTGAATGCAGTCCATG+AGG | 0.750934 | 8.4:+60427983 | MS.gene92389:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATGTTTTTAAAAATGAATT+AGG | - | chr8.4:60428534-60428553 | None:intergenic | 10.0% |
| !!! | TACTTTCTTTTTTTAAATTA+AGG | - | chr8.4:60428566-60428585 | None:intergenic | 10.0% |
| !! | TGAAAATAAACGTAAAAAAT+TGG | - | chr8.4:60428641-60428660 | None:intergenic | 15.0% |
| !! | ATACTGTAATAACTTAAATG+TGG | - | chr8.4:60428186-60428205 | None:intergenic | 20.0% |
| !! | TAAACCACAAATATTAAACA+AGG | - | chr8.4:60428279-60428298 | None:intergenic | 20.0% |
| !!! | TAAGAGTGTGTAATTATATA+TGG | + | chr8.4:60427731-60427750 | MS.gene92389:intron | 20.0% |
| !!! | TTTTCCTTGTTTAATATTTG+TGG | + | chr8.4:60428272-60428291 | MS.gene92389:intron | 20.0% |
| ! | AAGATATTTATTTGCGAGTT+AGG | + | chr8.4:60428402-60428421 | MS.gene92389:intron | 25.0% |
| !!! | GAGTGCTTTATTTTTGATAT+AGG | + | chr8.4:60428682-60428701 | MS.gene92389:intron | 25.0% |
| !!! | TGCAGGTTAGTTTTTTTTTA+AGG | + | chr8.4:60427592-60427611 | MS.gene92389:intron | 25.0% |
| AGGGCTAATACAAAAGATTA+TGG | + | chr8.4:60428764-60428783 | MS.gene92389:CDS | 30.0% | |
| CTCACTTGAAAACAAAGAAA+TGG | + | chr8.4:60428303-60428322 | MS.gene92389:intron | 30.0% | |
| GTAGAGTTTGTCTTGTATAT+GGG | + | chr8.4:60428325-60428344 | MS.gene92389:intron | 30.0% | |
| TATAGTATTAAGCACACACT+AGG | - | chr8.4:60427924-60427943 | None:intergenic | 30.0% | |
| TATCTGACACCATAAGATAA+AGG | - | chr8.4:60427629-60427648 | None:intergenic | 30.0% | |
| ! | TTTACAAGTTGAGTGTTAGA+TGG | - | chr8.4:60428596-60428615 | None:intergenic | 30.0% |
| !! | GAAAGTACTCATGAAATAGA+AGG | + | chr8.4:60428221-60428240 | MS.gene92389:intron | 30.0% |
| AAAGGAGGCTTAGAAAAAGT+TGG | - | chr8.4:60428806-60428825 | None:intergenic | 35.0% | |
| AATTGGAAACCATGCTCTTT+AGG | - | chr8.4:60428624-60428643 | None:intergenic | 35.0% | |
| AGCAAAGAAGTCAATGAATG+AGG | + | chr8.4:60428709-60428728 | MS.gene92389:CDS | 35.0% | |
| CAATTTGGTATGAGCTTGAA+AGG | - | chr8.4:60428824-60428843 | None:intergenic | 35.0% | |
| GGTAGAGTTTGTCTTGTATA+TGG | + | chr8.4:60428324-60428343 | MS.gene92389:intron | 35.0% | |
| GTGTCAGATACATGTTCTTA+TGG | + | chr8.4:60427639-60427658 | MS.gene92389:intron | 35.0% | |
| GTGTGCTTAATACTATATGC+AGG | + | chr8.4:60427927-60427946 | MS.gene92389:intron | 35.0% | |
| TAAACAAAGCCTAAAGAGCA+TGG | + | chr8.4:60428612-60428631 | MS.gene92389:intron | 35.0% | |
| TTCTTGCAGCCTTTATCTTA+TGG | + | chr8.4:60427617-60427636 | MS.gene92389:intron | 35.0% | |
| ! | ACCATTGATTGTTAGGTACA+AGG | - | chr8.4:60428146-60428165 | None:intergenic | 35.0% |
| ! | TATTCCATGATCTTCTGAAG+TGG | + | chr8.4:60427775-60427794 | MS.gene92389:intron | 35.0% |
| !! | ATTCCATGATCTTCTGAAGT+GGG | + | chr8.4:60427776-60427795 | MS.gene92389:intron | 35.0% |
| !! | GTTTTGCACCATTGATTGTT+AGG | - | chr8.4:60428153-60428172 | None:intergenic | 35.0% |
| !! | TCACTTTTGACAACTTTTGC+AGG | + | chr8.4:60427575-60427594 | MS.gene92389:CDS | 35.0% |
| ATTTGCGAGTTAGGTTTACG+AGG | + | chr8.4:60428411-60428430 | MS.gene92389:intron | 40.0% | |
| GAAGTGAATTTCCTACCTCA+TGG | - | chr8.4:60428001-60428020 | None:intergenic | 40.0% | |
| GCCTTGTACCTAACAATCAA+TGG | + | chr8.4:60428142-60428161 | MS.gene92389:intron | 40.0% | |
| TCAAAAGTGAGCCAGAAATG+AGG | - | chr8.4:60427565-60427584 | None:intergenic | 40.0% | |
| TTTGCGAGTTAGGTTTACGA+GGG | + | chr8.4:60428412-60428431 | MS.gene92389:intron | 40.0% | |
| TTTGGTATGAGCTTGAAAGG+AGG | - | chr8.4:60428821-60428840 | None:intergenic | 40.0% | |
| ! | CAAGTTGAGTGTTAGATGGA+AGG | - | chr8.4:60428592-60428611 | None:intergenic | 40.0% |
| !! | AAGTAAGAAGCATCCATGAG+AGG | + | chr8.4:60428735-60428754 | MS.gene92389:CDS | 40.0% |
| !! | GTACAAGGCTCTAAACATGA+TGG | - | chr8.4:60428131-60428150 | None:intergenic | 40.0% |
| AAAGTGAGCCAGAAATGAGG+AGG | - | chr8.4:60427562-60427581 | None:intergenic | 45.0% | |
| AGATGTGAATGCAGTCCATG+AGG | + | chr8.4:60427983-60428002 | MS.gene92389:CDS | 45.0% | |
| CATCCATGAGAGGCTTCTTA+GGG | + | chr8.4:60428745-60428764 | MS.gene92389:CDS | 45.0% | |
| CTTCCCACTTCAGAAGATCA+TGG | - | chr8.4:60427782-60427801 | None:intergenic | 45.0% | |
| CTTCTCTTCCTCCTCATTTC+TGG | + | chr8.4:60427551-60427570 | MS.gene92389:CDS | 45.0% | |
| GGCTTAGAAAAAGTTGGCGA+TGG | - | chr8.4:60428800-60428819 | None:intergenic | 45.0% | |
| !!! | AGGTTTACGAGGGAGTGTTT+TGG | + | chr8.4:60428422-60428441 | MS.gene92389:intron | 45.0% |
| GCATCCATGAGAGGCTTCTT+AGG | + | chr8.4:60428744-60428763 | MS.gene92389:CDS | 50.0% | |
| GTGAATGCAGTCCATGAGGT+AGG | + | chr8.4:60427987-60428006 | MS.gene92389:intron | 50.0% | |
| TAGCCCTAAGAAGCCTCTCA+TGG | - | chr8.4:60428751-60428770 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 60427521 | 60428844 | 60427521 | ID=MS.gene92389 |
| chr8.4 | mRNA | 60427521 | 60428844 | 60427521 | ID=MS.gene92389.t1;Parent=MS.gene92389 |
| chr8.4 | exon | 60427521 | 60427596 | 60427521 | ID=MS.gene92389.t1.exon1;Parent=MS.gene92389.t1 |
| chr8.4 | CDS | 60427521 | 60427596 | 60427521 | ID=cds.MS.gene92389.t1;Parent=MS.gene92389.t1 |
| chr8.4 | exon | 60427949 | 60428004 | 60427949 | ID=MS.gene92389.t1.exon2;Parent=MS.gene92389.t1 |
| chr8.4 | CDS | 60427949 | 60428004 | 60427949 | ID=cds.MS.gene92389.t1;Parent=MS.gene92389.t1 |
| chr8.4 | exon | 60428704 | 60428844 | 60428704 | ID=MS.gene92389.t1.exon3;Parent=MS.gene92389.t1 |
| chr8.4 | CDS | 60428704 | 60428844 | 60428704 | ID=cds.MS.gene92389.t1;Parent=MS.gene92389.t1 |
| Gene Sequence |
| Protein sequence |
