Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94255.t1 | XP_003623166.1 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.90E-56 | 227.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94255.t1 | G7L5L7 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 2.1e-56 | 227.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050904 | MS.gene94255 | -0.818418 | 1.98E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene94255 | 0.809258 | 2.07E-50 | -1.69E-46 |
| MS.gene052606 | MS.gene94255 | 0.817677 | 2.91E-52 | -1.69E-46 |
| MS.gene053108 | MS.gene94255 | 0.82537 | 4.86E-54 | -1.69E-46 |
| MS.gene055157 | MS.gene94255 | 0.804764 | 1.85E-49 | -1.69E-46 |
| MS.gene055158 | MS.gene94255 | 0.952691 | 1.40E-110 | -1.69E-46 |
| MS.gene055159 | MS.gene94255 | 0.881209 | 2.90E-70 | -1.69E-46 |
| MS.gene055748 | MS.gene94255 | -0.810109 | 1.36E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene94255 | -0.801766 | 7.71E-49 | -1.69E-46 |
| MS.gene056387 | MS.gene94255 | -0.807089 | 5.99E-50 | -1.69E-46 |
| MS.gene056678 | MS.gene94255 | 0.811424 | 7.05E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene94255 | 0.819226 | 1.30E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene94255 | 0.800571 | 1.35E-48 | -1.69E-46 |
| MS.gene057768 | MS.gene94255 | 0.805182 | 1.51E-49 | -1.69E-46 |
| MS.gene058158 | MS.gene94255 | 0.81966 | 1.03E-52 | -1.69E-46 |
| MS.gene058418 | MS.gene94255 | 0.823278 | 1.51E-53 | -1.69E-46 |
| MS.gene059526 | MS.gene94255 | 0.80493 | 1.71E-49 | -1.69E-46 |
| MS.gene05960 | MS.gene94255 | 0.835539 | 1.58E-56 | -1.69E-46 |
| MS.gene06000 | MS.gene94255 | -0.812028 | 5.21E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94255.t1 | MTR_7g065210 | 93.162 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 1.01e-78 | 227 |
| MS.gene94255.t1 | MTR_7g065160 | 88.889 | 117 | 13 | 0 | 1 | 117 | 1 | 117 | 8.18e-75 | 217 |
| MS.gene94255.t1 | MTR_8g070040 | 45.455 | 121 | 61 | 3 | 1 | 117 | 1 | 120 | 9.40e-26 | 93.6 |
| MS.gene94255.t1 | MTR_8g070015 | 42.105 | 114 | 61 | 3 | 1 | 110 | 1 | 113 | 5.03e-20 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94255.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene94255.t1 | AT3G53980 | 68.421 | 95 | 29 | 1 | 23 | 117 | 21 | 114 | 2.24e-44 | 140 |
| MS.gene94255.t1 | AT5G05960 | 58.120 | 117 | 44 | 2 | 5 | 117 | 1 | 116 | 8.88e-42 | 134 |
| MS.gene94255.t1 | AT2G37870 | 44.144 | 111 | 60 | 2 | 8 | 117 | 6 | 115 | 2.07e-25 | 92.8 |
Find 24 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTAAATGCTTCCATATC+TGG | 0.265044 | 7.3:-40770376 | None:intergenic |
| TAGCAAAGTTGCAGCGTTTA+GGG | 0.296288 | 7.3:-40770561 | None:intergenic |
| TTAGCAAAGTTGCAGCGTTT+AGG | 0.351023 | 7.3:-40770562 | None:intergenic |
| GGTATTGCAGTTCTTGTTGC+AGG | 0.361788 | 7.3:+40770307 | MS.gene94255:CDS |
| CGTCCACACTTATAACCAAT+GGG | 0.425846 | 7.3:-40770589 | None:intergenic |
| TCAAACGTTGCTAAAATGTC+TGG | 0.462464 | 7.3:+40770511 | MS.gene94255:CDS |
| AAGGGGTACTACACCAGATA+TGG | 0.470603 | 7.3:+40770363 | MS.gene94255:CDS |
| GGATAGTAACAGCAATTTGA+GGG | 0.472152 | 7.3:-40770540 | None:intergenic |
| GGGATAGTAACAGCAATTTG+AGG | 0.508534 | 7.3:-40770541 | None:intergenic |
| CGAGGCAAAACAATTTCATA+AGG | 0.511907 | 7.3:-40770282 | None:intergenic |
| GTTGAAAGTGCAGGTGAATG+TGG | 0.515102 | 7.3:+40770340 | MS.gene94255:CDS |
| AACTTTGCTAATCGCCCCAT+TGG | 0.518869 | 7.3:+40770574 | MS.gene94255:CDS |
| ACGTCCACACTTATAACCAA+TGG | 0.527913 | 7.3:-40770590 | None:intergenic |
| TCCTTGTGCAAGTGCAGCAC+AGG | 0.530578 | 7.3:+40770402 | MS.gene94255:CDS |
| AAAGTGCAGGTGAATGTGGA+AGG | 0.549386 | 7.3:+40770344 | MS.gene94255:CDS |
| TCCTGTGCTGCACTTGCACA+AGG | 0.569239 | 7.3:-40770403 | None:intergenic |
| CGCCCCATTGGTTATAAGTG+TGG | 0.571913 | 7.3:+40770586 | MS.gene94255:CDS |
| TGTGATCAGACTAAGAAACT+TGG | 0.574065 | 7.3:+40770454 | MS.gene94255:CDS |
| ATTCATAGAGTTGAAAGTGC+AGG | 0.578390 | 7.3:+40770331 | MS.gene94255:CDS |
| AAAACTGCACAAAGACAACT+TGG | 0.590774 | 7.3:-40770484 | None:intergenic |
| AGTGCAGGTGAATGTGGAAG+GGG | 0.630405 | 7.3:+40770346 | MS.gene94255:CDS |
| AAGTGCAGGTGAATGTGGAA+GGG | 0.641454 | 7.3:+40770345 | MS.gene94255:CDS |
| CAAGAACTGCAATACCAACG+AGG | 0.744882 | 7.3:-40770300 | None:intergenic |
| GTCCACACTTATAACCAATG+GGG | 0.748420 | 7.3:-40770588 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAACTTTTATATGAAAA+CGG | - | chr7.3:40770652-40770671 | None:intergenic | 10.0% |
| !!! | TTTTAACAATAAATTTTCTT+AGG | - | chr7.3:40770779-40770798 | None:intergenic | 10.0% |
| !! | ATGTAACATTAATAATTGAT+AGG | + | chr7.3:40770704-40770723 | MS.gene94255:intron | 15.0% |
| !!! | GTTTTCATATAAAAGTTATT+TGG | + | chr7.3:40770651-40770670 | MS.gene94255:intron | 15.0% |
| !! | AATGAGATTATAGATAGTTA+AGG | + | chr7.3:40770730-40770749 | MS.gene94255:intron | 20.0% |
| !! | ATGAGATTATAGATAGTTAA+GGG | + | chr7.3:40770731-40770750 | MS.gene94255:intron | 20.0% |
| !!! | CTATTTGTCAATTTTTTTGA+TGG | + | chr7.3:40770888-40770907 | MS.gene94255:intron | 20.0% |
| ! | TTGTTAAAATCTTCCCTTTA+TGG | + | chr7.3:40770790-40770809 | MS.gene94255:intron | 25.0% |
| !!! | TAGATAGTTAAGGGTATTAT+AGG | + | chr7.3:40770740-40770759 | MS.gene94255:intron | 25.0% |
| TGCCTAAGAAAAATGTGAAA+CGG | + | chr7.3:40770837-40770856 | MS.gene94255:intron | 30.0% | |
| ! | TACCGTTTCACATTTTTCTT+AGG | - | chr7.3:40770842-40770861 | None:intergenic | 30.0% |
| !! | CTAGTTTCTAGTTCCATAAA+GGG | - | chr7.3:40770806-40770825 | None:intergenic | 30.0% |
| !! | TCTAGTTTCTAGTTCCATAA+AGG | - | chr7.3:40770807-40770826 | None:intergenic | 30.0% |
| AAAACTGCACAAAGACAACT+TGG | - | chr7.3:40770487-40770506 | None:intergenic | 35.0% | |
| AGCTTAAATGCTTCCATATC+TGG | - | chr7.3:40770379-40770398 | None:intergenic | 35.0% | |
| CGAGGCAAAACAATTTCATA+AGG | - | chr7.3:40770285-40770304 | None:intergenic | 35.0% | |
| GGATAGTAACAGCAATTTGA+GGG | - | chr7.3:40770543-40770562 | None:intergenic | 35.0% | |
| TAAGTAACTAACAAGTCCCT+CGG | + | chr7.3:40770612-40770631 | MS.gene94255:intron | 35.0% | |
| TCAAACGTTGCTAAAATGTC+TGG | + | chr7.3:40770511-40770530 | MS.gene94255:CDS | 35.0% | |
| TGTGATCAGACTAAGAAACT+TGG | + | chr7.3:40770454-40770473 | MS.gene94255:CDS | 35.0% | |
| ! | ATTCATAGAGTTGAAAGTGC+AGG | + | chr7.3:40770331-40770350 | MS.gene94255:CDS | 35.0% |
| !! | ATGAAATTGTTTTGCCTCGT+TGG | + | chr7.3:40770286-40770305 | MS.gene94255:CDS | 35.0% |
| ACGTCCACACTTATAACCAA+TGG | - | chr7.3:40770593-40770612 | None:intergenic | 40.0% | |
| CGTCCACACTTATAACCAAT+GGG | - | chr7.3:40770592-40770611 | None:intergenic | 40.0% | |
| GGCAACTTAAAAGAAACCGA+GGG | - | chr7.3:40770631-40770650 | None:intergenic | 40.0% | |
| GGGATAGTAACAGCAATTTG+AGG | - | chr7.3:40770544-40770563 | None:intergenic | 40.0% | |
| GTCCACACTTATAACCAATG+GGG | - | chr7.3:40770591-40770610 | None:intergenic | 40.0% | |
| TAGCAAAGTTGCAGCGTTTA+GGG | - | chr7.3:40770564-40770583 | None:intergenic | 40.0% | |
| TTAGCAAAGTTGCAGCGTTT+AGG | - | chr7.3:40770565-40770584 | None:intergenic | 40.0% | |
| AAAGTGCAGGTGAATGTGGA+AGG | + | chr7.3:40770344-40770363 | MS.gene94255:CDS | 45.0% | |
| AACTTTGCTAATCGCCCCAT+TGG | + | chr7.3:40770574-40770593 | MS.gene94255:CDS | 45.0% | |
| AAGTGCAGGTGAATGTGGAA+GGG | + | chr7.3:40770345-40770364 | MS.gene94255:CDS | 45.0% | |
| CAAGAACTGCAATACCAACG+AGG | - | chr7.3:40770303-40770322 | None:intergenic | 45.0% | |
| CGGCAACTTAAAAGAAACCG+AGG | - | chr7.3:40770632-40770651 | None:intergenic | 45.0% | |
| GGTATTGCAGTTCTTGTTGC+AGG | + | chr7.3:40770307-40770326 | MS.gene94255:CDS | 45.0% | |
| ! | GTTGAAAGTGCAGGTGAATG+TGG | + | chr7.3:40770340-40770359 | MS.gene94255:CDS | 45.0% |
| !! | AAGGGGTACTACACCAGATA+TGG | + | chr7.3:40770363-40770382 | MS.gene94255:CDS | 45.0% |
| AGTGCAGGTGAATGTGGAAG+GGG | + | chr7.3:40770346-40770365 | MS.gene94255:CDS | 50.0% | |
| ! | CGCCCCATTGGTTATAAGTG+TGG | + | chr7.3:40770586-40770605 | MS.gene94255:CDS | 50.0% |
| TCCTGTGCTGCACTTGCACA+AGG | - | chr7.3:40770406-40770425 | None:intergenic | 55.0% | |
| TCCTTGTGCAAGTGCAGCAC+AGG | + | chr7.3:40770402-40770421 | MS.gene94255:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40770274 | 40770968 | 40770274 | ID=MS.gene94255 |
| chr7.3 | mRNA | 40770274 | 40770968 | 40770274 | ID=MS.gene94255.t1;Parent=MS.gene94255 |
| chr7.3 | exon | 40770274 | 40770610 | 40770274 | ID=MS.gene94255.t1.exon1;Parent=MS.gene94255.t1 |
| chr7.3 | CDS | 40770274 | 40770610 | 40770274 | ID=cds.MS.gene94255.t1;Parent=MS.gene94255.t1 |
| chr7.3 | exon | 40770952 | 40770968 | 40770952 | ID=MS.gene94255.t1.exon2;Parent=MS.gene94255.t1 |
| chr7.3 | CDS | 40770952 | 40770968 | 40770952 | ID=cds.MS.gene94255.t1;Parent=MS.gene94255.t1 |
| Gene Sequence |
| Protein sequence |
