AlfalfaGEDB | MS.gene97447

M. sativa cultivar XinJiangDaYe / MS.gene97447

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97447.t1	AFK42063.1	97.6	84	2	0	1	84	63	146	1.90E-41	177.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97447.t1	Q9FY93	77.5	80	18	0	1	80	62	141	2.5e-34	145.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97447.t1	G7KVL9	97.6	84	2	0	1	84	63	146	1.4e-41	177.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene97447	0.813357	2.67E-51	-1.69E-46
MS.gene049983	MS.gene97447	0.807715	4.41E-50	-1.69E-46
MS.gene051541	MS.gene97447	0.811694	6.16E-51	-1.69E-46
MS.gene052586	MS.gene97447	0.810602	1.06E-50	-1.69E-46
MS.gene052685	MS.gene97447	0.834404	3.06E-56	-1.69E-46
MS.gene053298	MS.gene97447	0.815648	8.30E-52	-1.69E-46
MS.gene053963	MS.gene97447	0.867704	1.12E-65	-1.69E-46
MS.gene054222	MS.gene97447	0.812024	5.22E-51	-1.69E-46
MS.gene054479	MS.gene97447	0.813678	2.27E-51	-1.69E-46
MS.gene054892	MS.gene97447	0.823675	1.22E-53	-1.69E-46
MS.gene054893	MS.gene97447	0.819181	1.33E-52	-1.69E-46
MS.gene055147	MS.gene97447	0.804572	2.03E-49	-1.69E-46
MS.gene058159	MS.gene97447	0.828573	8.34E-55	-1.69E-46
MS.gene058429	MS.gene97447	0.825491	4.56E-54	-1.69E-46
MS.gene05878	MS.gene97447	0.802203	6.27E-49	-1.69E-46
MS.gene05952	MS.gene97447	0.863878	1.82E-64	-1.69E-46
MS.gene05956	MS.gene97447	0.809414	1.91E-50	-1.69E-46
MS.gene060012	MS.gene97447	0.818671	1.74E-52	-1.69E-46
MS.gene060412	MS.gene97447	0.839958	1.16E-57	-1.69E-46
MS.gene060840	MS.gene97447	0.812214	4.75E-51	-1.69E-46
MS.gene060879	MS.gene97447	0.817792	2.74E-52	-1.69E-46
MS.gene060932	MS.gene97447	0.801684	8.02E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 19 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGGTTCCCATTTGGGTATT+TGG	0.189837	7.4:-94484074	None:intergenic
CTGTTAGATCGGTTCCCATT+TGG	0.268180	7.4:-94484082	None:intergenic
TGTTAGATCGGTTCCCATTT+GGG	0.328133	7.4:-94484081	None:intergenic
GAGCTACAAACTCTGGTTAC+TGG	0.339051	7.4:+94484104	MS.gene97447:CDS
AAAGAAGCCAAATACCCAAA+TGG	0.451382	7.4:+94484067	MS.gene97447:CDS
TCTGGTTACTGGAAAGCAAC+TGG	0.454286	7.4:+94484115	MS.gene97447:CDS
TCAAAGAGTCATGAAGTTGT+TGG	0.455914	7.4:+94484160	MS.gene97447:CDS
AAGAAGCCAAATACCCAAAT+GGG	0.481949	7.4:+94484068	MS.gene97447:CDS
TTCCTCTCACTCCAACCCCT+TGG	0.484311	7.4:+94484267	MS.gene97447:CDS
GGTTGGAGTGAGAGGAAGTG+AGG	0.514880	7.4:-94484261	None:intergenic
AGTTTGTAGCTCTGTTAGAT+CGG	0.532621	7.4:-94484093	None:intergenic
GACCAAGGGGTTGGAGTGAG+AGG	0.548262	7.4:-94484269	None:intergenic
CTCATGGTTCAAGAACTGAT+TGG	0.548704	7.4:+94484221	MS.gene97447:CDS
AGAAAGACTGACCAAGGGGT+TGG	0.548887	7.4:-94484278	None:intergenic
TCTAACAGAGCTACAAACTC+TGG	0.560803	7.4:+94484097	MS.gene97447:CDS
TACAGAGGCAAACCACCTCA+TGG	0.581454	7.4:+94484205	MS.gene97447:CDS
TCAGTTCTTGAACCATGAGG+TGG	0.615010	7.4:-94484217	None:intergenic
TAGGTGATATGGAACAAGAG+AGG	0.664397	7.4:+94484029	None:intergenic
CAATCAGTTCTTGAACCATG+AGG	0.707588	7.4:-94484220	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAACTCTTGTTTTCTACAG+AGG	+	chr7.4:94484190-94484209	MS.gene97447:CDS	30.0%
	AAAGAAGCCAAATACCCAAA+TGG	+	chr7.4:94484067-94484086	MS.gene97447:CDS	35.0%
	AAGAAGCCAAATACCCAAAT+GGG	+	chr7.4:94484068-94484087	MS.gene97447:CDS	35.0%
	TCAAAGAGTCATGAAGTTGT+TGG	+	chr7.4:94484160-94484179	MS.gene97447:CDS	35.0%
!	AGTTTGTAGCTCTGTTAGAT+CGG	-	chr7.4:94484096-94484115	None:intergenic	35.0%
	CAATCAGTTCTTGAACCATG+AGG	-	chr7.4:94484223-94484242	None:intergenic	40.0%
	CTCATGGTTCAAGAACTGAT+TGG	+	chr7.4:94484221-94484240	MS.gene97447:CDS	40.0%
	TCTAACAGAGCTACAAACTC+TGG	+	chr7.4:94484097-94484116	MS.gene97447:CDS	40.0%
!	TGTTAGATCGGTTCCCATTT+GGG	-	chr7.4:94484084-94484103	None:intergenic	40.0%
	GAGCTACAAACTCTGGTTAC+TGG	+	chr7.4:94484104-94484123	MS.gene97447:CDS	45.0%
	TCAGTTCTTGAACCATGAGG+TGG	-	chr7.4:94484220-94484239	None:intergenic	45.0%
	TCGGTTCCCATTTGGGTATT+TGG	-	chr7.4:94484077-94484096	None:intergenic	45.0%
	TCTGGTTACTGGAAAGCAAC+TGG	+	chr7.4:94484115-94484134	MS.gene97447:CDS	45.0%
!	CTGTTAGATCGGTTCCCATT+TGG	-	chr7.4:94484085-94484104	None:intergenic	45.0%
	TACAGAGGCAAACCACCTCA+TGG	+	chr7.4:94484205-94484224	MS.gene97447:CDS	50.0%
	GGTTGGAGTGAGAGGAAGTG+AGG	-	chr7.4:94484264-94484283	None:intergenic	55.0%
	TTCCTCTCACTCCAACCCCT+TGG	+	chr7.4:94484267-94484286	MS.gene97447:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	94484032	94484288	94484032	ID=MS.gene97447
chr7.4	mRNA	94484032	94484288	94484032	ID=MS.gene97447.t1;Parent=MS.gene97447
chr7.4	exon	94484032	94484288	94484032	ID=MS.gene97447.t1.exon1;Parent=MS.gene97447.t1
chr7.4	CDS	94484032	94484288	94484032	ID=cds.MS.gene97447.t1;Parent=MS.gene97447.t1

Gene Sequence	>MS.gene97447 GTGATATGGAACAAGAGAGGTACTTTTTCAGTACTAAAGAAGCCAAATACCCAAATGGGAACCGATCTAACAGAGCTACAAACTCTGGTTACTGGAAAGCAACTGGTTTAGACAAACAAATCATGAATTCAAAGAGTCATGAAGTTGTTGGTATGAAGAAAACTCTTGTTTTCTACAGAGGCAAACCACCTCATGGTTCAAGAACTGATTGGATCATGCATGAATATCGCCTCACTTCCTCTCACTCCAACCCCTTG
Protein sequence	>MS.gene97447.t1 VIWNKRGTFSVLKKPNTQMGTDLTELQTLVTGKQLVUTNKSUIQRVMKLLVURKLLFSTEANHLMVQELIGSCMNIASLPLTPTP