AlfalfaGEDB | MS.gene97467

M. sativa cultivar XinJiangDaYe / MS.gene97467

KEGG: K14488
GO: GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0050896
PFAM: PF02519.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97467.t1	XP_013441979.1	94.7	150	8	0	1	150	1	150	2.70E-78	301.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97467.t1	O22150	48.7	154	69	4	1	147	1	151	3.1e-35	149.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97467.t1	G7J151	94.7	150	8	0	1	150	1	150	1.9e-78	301.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049396	MS.gene97467	0.825031	5.85E-54	-1.69E-46
MS.gene050502	MS.gene97467	0.827506	1.51E-54	-1.69E-46
MS.gene051941	MS.gene97467	0.803792	2.94E-49	-1.69E-46
MS.gene052346	MS.gene97467	0.836946	6.94E-57	-1.69E-46
MS.gene052414	MS.gene97467	0.852443	4.60E-61	-1.69E-46
MS.gene053169	MS.gene97467	0.809247	2.08E-50	-1.69E-46
MS.gene054486	MS.gene97467	0.802436	5.61E-49	-1.69E-46
MS.gene055369	MS.gene97467	0.835197	1.93E-56	-1.69E-46
MS.gene055741	MS.gene97467	0.825459	4.63E-54	-1.69E-46
MS.gene055744	MS.gene97467	0.828273	9.85E-55	-1.69E-46
MS.gene055909	MS.gene97467	0.858813	6.36E-63	-1.69E-46
MS.gene055911	MS.gene97467	0.863526	2.34E-64	-1.69E-46
MS.gene056166	MS.gene97467	0.815368	9.58E-52	-1.69E-46
MS.gene056346	MS.gene97467	0.83492	2.27E-56	-1.69E-46
MS.gene056511	MS.gene97467	0.816444	5.51E-52	-1.69E-46
MS.gene056514	MS.gene97467	0.845977	2.90E-59	-1.69E-46
MS.gene056877	MS.gene97467	0.813894	2.03E-51	-1.69E-46
MS.gene057019	MS.gene97467	0.814946	1.19E-51	-1.69E-46
MS.gene057086	MS.gene97467	0.820834	5.56E-53	-1.69E-46
MS.gene057749	MS.gene97467	0.81249	4.13E-51	-1.69E-46
MS.gene057823	MS.gene97467	0.80269	4.98E-49	-1.69E-46
MS.gene057856	MS.gene97467	0.801212	1.00E-48	-1.69E-46
MS.gene059368	MS.gene97467	0.800465	1.42E-48	-1.69E-46
MS.gene059420	MS.gene97467	0.80619	9.28E-50	-1.69E-46
MS.gene059674	MS.gene97467	0.812717	3.68E-51	-1.69E-46
MS.gene060372	MS.gene97467	0.823181	1.59E-53	-1.69E-46
MS.gene060485	MS.gene97467	0.816204	6.24E-52	-1.69E-46
MS.gene060835	MS.gene97467	0.824791	6.66E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97467.t1	MTR_0388s0020	94.667	150	8	0	1	150	1	150	3.56e-106	299
MS.gene97467.t1	MTR_4g025830	53.289	152	57	4	7	147	3	151	2.56e-47	150
MS.gene97467.t1	MTR_7g118010	60.294	68	26	1	74	141	22	88	7.18e-24	89.7
MS.gene97467.t1	MTR_3g092220	50.000	74	36	1	74	147	32	104	2.11e-20	80.9
MS.gene97467.t1	MTR_8g026730	55.882	68	29	1	74	141	26	92	4.55e-20	80.1
MS.gene97467.t1	MTR_3g084250	49.398	83	37	3	61	141	10	89	1.08e-18	75.9
MS.gene97467.t1	MTR_4g072240	44.706	85	42	3	57	139	7	88	1.08e-17	73.6
MS.gene97467.t1	MTR_4g072500	42.045	88	46	3	56	141	5	89	2.34e-17	72.4
MS.gene97467.t1	MTR_4g072570	45.783	83	40	3	61	141	10	89	2.61e-17	72.4
MS.gene97467.t1	MTR_4g072300	44.706	85	42	3	57	139	7	88	3.12e-17	72.0
MS.gene97467.t1	MTR_4g072870	41.860	86	45	3	58	141	8	90	5.18e-17	71.6
MS.gene97467.t1	MTR_4g072530	40.909	88	47	3	56	141	5	89	7.13e-17	71.2
MS.gene97467.t1	MTR_4g072980	39.080	87	50	2	56	141	8	92	1.05e-16	70.9
MS.gene97467.t1	MTR_4g072220	40.909	88	47	3	57	142	7	91	1.09e-16	70.9
MS.gene97467.t1	MTR_4g072640	39.773	88	48	3	56	141	5	89	1.39e-16	70.5
MS.gene97467.t1	MTR_4g072600	43.590	78	40	2	66	142	11	85	5.17e-16	68.9
MS.gene97467.t1	MTR_4g072190	37.755	98	55	3	46	141	6	99	6.19e-16	68.9
MS.gene97467.t1	MTR_3g117640	44.595	74	38	2	74	146	33	104	8.05e-16	68.9
MS.gene97467.t1	MTR_4g072860	46.377	69	33	2	74	141	25	90	1.37e-15	68.2
MS.gene97467.t1	MTR_4g072330	44.000	75	38	2	68	141	16	87	3.33e-15	67.0
MS.gene97467.t1	MTR_4g072850	46.269	67	32	2	74	139	26	89	4.72e-15	66.6
MS.gene97467.t1	MTR_3g084210	50.000	60	26	2	72	130	22	78	5.16e-15	66.6
MS.gene97467.t1	MTR_3g084230	50.000	60	26	2	72	130	22	78	5.87e-15	66.2
MS.gene97467.t1	MTR_3g084160	51.667	60	25	2	72	130	22	78	7.52e-15	66.2
MS.gene97467.t1	MTR_8g076040	48.529	68	32	1	74	141	40	104	7.53e-15	66.6
MS.gene97467.t1	MTR_4g072910	49.275	69	30	3	62	129	23	87	8.55e-15	66.2
MS.gene97467.t1	MTR_4g072230	43.662	71	34	2	70	139	21	86	1.03e-14	65.9
MS.gene97467.t1	MTR_8g461420	50.000	60	26	2	72	130	22	78	1.36e-14	65.5
MS.gene97467.t1	MTR_3g113310	42.857	70	37	1	72	141	39	105	1.39e-14	65.9
MS.gene97467.t1	MTR_3g117610	42.029	69	37	1	73	141	43	108	1.69e-14	65.9
MS.gene97467.t1	MTR_3g084190	51.667	60	25	2	72	130	22	78	1.70e-14	65.1
MS.gene97467.t1	MTR_4g072310	40.541	74	40	2	69	141	20	90	1.72e-14	65.1
MS.gene97467.t1	MTR_4g072270	44.444	72	36	2	69	139	20	88	2.05e-14	65.1
MS.gene97467.t1	MTR_4g072480	43.373	83	42	3	61	141	10	89	2.18e-14	65.1
MS.gene97467.t1	MTR_5g021820	41.975	81	42	2	61	141	28	103	2.34e-14	65.1
MS.gene97467.t1	MTR_4g072880	40.476	84	45	3	58	139	8	88	2.65e-14	64.7
MS.gene97467.t1	MTR_3g110038	41.250	80	43	2	61	139	11	87	2.67e-14	64.7
MS.gene97467.t1	MTR_3g117630	46.479	71	33	3	61	129	10	77	2.76e-14	64.7
MS.gene97467.t1	MTR_4g072560	51.724	58	25	2	74	130	31	86	2.98e-14	64.7
MS.gene97467.t1	MTR_4g072890	42.500	80	41	3	57	134	7	83	4.26e-14	64.3
MS.gene97467.t1	MTR_4g072620	40.000	85	47	2	58	141	8	89	5.30e-14	63.9
MS.gene97467.t1	MTR_4g072370	43.243	74	38	2	69	141	17	87	5.74e-14	63.9
MS.gene97467.t1	MTR_3g084240	40.000	80	44	2	61	139	11	87	5.84e-14	63.9
MS.gene97467.t1	MTR_4g072260	37.079	89	51	3	57	143	7	92	8.08e-14	63.5
MS.gene97467.t1	MTR_4g072250	43.284	67	33	2	74	139	25	87	8.23e-14	63.5
MS.gene97467.t1	MTR_3g084170	40.000	80	44	2	61	139	11	87	9.06e-14	63.2
MS.gene97467.t1	MTR_4g072730	43.836	73	37	2	70	141	20	89	9.46e-14	63.2
MS.gene97467.t1	MTR_3g084220	44.286	70	35	2	61	129	11	77	9.66e-14	63.2
MS.gene97467.t1	MTR_3g110045	50.000	58	25	2	74	130	83	137	1.06e-13	64.7
MS.gene97467.t1	MTR_4g072820	42.647	68	35	2	75	141	20	84	1.08e-13	62.8
MS.gene97467.t1	MTR_4g072740	42.857	77	40	2	66	141	11	84	1.30e-13	62.8
MS.gene97467.t1	MTR_3g084150	41.975	81	42	3	61	139	10	87	1.44e-13	62.8
MS.gene97467.t1	MTR_3g084180	41.975	81	42	3	61	139	10	87	1.58e-13	62.8
MS.gene97467.t1	MTR_4g072550	39.175	97	54	3	42	137	4	96	1.74e-13	62.8
MS.gene97467.t1	MTR_4g072660	44.444	72	36	2	70	140	15	83	2.03e-13	62.4
MS.gene97467.t1	MTR_4g072680	45.570	79	38	3	61	137	10	85	2.05e-13	62.4
MS.gene97467.t1	MTR_4g072490	42.857	77	40	2	66	141	11	84	2.40e-13	62.0
MS.gene97467.t1	MTR_4g072460	45.570	79	38	3	61	137	10	85	2.65e-13	62.0
MS.gene97467.t1	MTR_4g072590	49.231	65	26	3	74	134	19	80	2.82e-13	62.0
MS.gene97467.t1	MTR_4g072930	38.542	96	51	4	41	134	13	102	2.95e-13	62.4
MS.gene97467.t1	MTR_4g072580	39.744	78	43	2	66	142	11	85	2.96e-13	62.0
MS.gene97467.t1	MTR_4g072940	46.154	65	31	2	74	137	56	117	3.07e-13	62.8
MS.gene97467.t1	MTR_4g072830	42.105	76	40	2	68	142	16	88	4.12e-13	61.6
MS.gene97467.t1	MTR_4g072630	44.776	67	33	2	65	130	11	74	4.25e-13	61.2
MS.gene97467.t1	MTR_4g124750	40.260	77	41	2	69	143	61	134	4.82e-13	63.2
MS.gene97467.t1	MTR_8g461410	40.741	81	43	3	61	139	10	87	5.03e-13	61.2
MS.gene97467.t1	MTR_2g043960	41.096	73	40	1	71	143	72	141	5.98e-13	62.8
MS.gene97467.t1	MTR_5g008370	40.000	75	41	2	74	147	24	95	1.05e-12	61.6
MS.gene97467.t1	MTR_4g072610	37.647	85	49	2	59	142	3	84	1.44e-12	60.1
MS.gene97467.t1	MTR_1g009150	40.541	74	41	1	74	147	70	140	1.47e-12	62.4
MS.gene97467.t1	MTR_5g008360	38.961	77	43	2	66	141	7	80	1.52e-12	60.1
MS.gene97467.t1	MTR_4g072540	42.029	69	36	2	74	141	20	85	1.57e-12	60.1
MS.gene97467.t1	MTR_4g072160	37.374	99	42	3	44	142	23	101	1.63e-12	61.2
MS.gene97467.t1	MTR_4g057810	43.750	64	35	1	74	137	37	99	1.71e-12	60.8
MS.gene97467.t1	MTR_4g072670	43.478	69	35	2	74	141	20	85	1.73e-12	59.7
MS.gene97467.t1	MTR_2g095430	43.860	57	32	0	81	137	52	108	1.92e-12	60.8
MS.gene97467.t1	MTR_5g008350	44.737	76	37	3	59	132	3	75	3.44e-12	58.9
MS.gene97467.t1	MTR_3g084260	41.250	80	40	3	61	139	15	88	4.56e-12	58.9
MS.gene97467.t1	MTR_1g024085	39.326	89	49	3	60	147	23	107	5.12e-12	60.1
MS.gene97467.t1	MTR_4g072720	42.029	69	36	2	74	141	20	85	5.36e-12	58.5
MS.gene97467.t1	MTR_4g072465	41.791	67	35	2	65	130	11	74	7.15e-12	58.2
MS.gene97467.t1	MTR_8g461210	40.909	66	36	1	72	137	32	94	7.87e-12	58.9
MS.gene97467.t1	MTR_3g109160	37.500	88	50	2	52	137	44	128	8.50e-12	60.1
MS.gene97467.t1	MTR_4g005320	42.857	63	33	1	75	137	70	129	8.91e-12	59.7
MS.gene97467.t1	MTR_4g072470	44.615	65	32	2	66	129	11	72	1.27e-11	57.4
MS.gene97467.t1	MTR_4g072510	41.429	70	37	2	73	141	19	85	1.46e-11	57.4
MS.gene97467.t1	MTR_4g072650	36.471	85	50	2	59	142	3	84	1.54e-11	57.4
MS.gene97467.t1	MTR_2g066830	32.558	86	55	1	59	144	42	124	1.86e-11	58.5
MS.gene97467.t1	MTR_4g072450	42.647	68	35	2	75	141	20	84	2.14e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene97467.t1	AT2G45210	48.701	154	69	4	1	147	1	151	1.12e-43	142
MS.gene97467.t1	AT3G60690	40.506	158	81	5	1	145	1	158	3.47e-36	123
MS.gene97467.t1	AT4G22620	40.816	147	83	3	1	143	1	147	6.95e-32	111
MS.gene97467.t1	AT4G12410	38.312	154	74	4	1	143	1	144	1.94e-29	105
MS.gene97467.t1	AT2G46690	48.052	77	40	0	74	150	23	99	1.39e-21	84.3
MS.gene97467.t1	AT3G61900	54.412	68	31	0	74	141	29	96	7.40e-21	82.8
MS.gene97467.t1	AT4G00880	50.000	74	36	1	75	148	28	100	1.65e-19	79.0
MS.gene97467.t1	AT5G53590	41.772	79	44	1	74	150	45	123	1.92e-17	74.3
MS.gene97467.t1	AT4G34800	55.738	61	26	1	75	134	21	81	2.97e-17	72.4
MS.gene97467.t1	AT3G03820	46.667	75	39	1	68	141	20	94	5.85e-17	71.6
MS.gene97467.t1	AT4G34810	48.148	81	40	2	52	130	25	105	8.51e-17	72.0
MS.gene97467.t1	AT4G38850	46.377	69	36	1	74	141	20	88	1.74e-16	70.1
MS.gene97467.t1	AT2G21210	42.308	78	43	2	69	145	22	98	1.95e-16	70.5
MS.gene97467.t1	AT4G34790	55.738	61	25	2	75	134	39	98	4.64e-16	69.7
MS.gene97467.t1	AT4G34770	46.250	80	40	3	67	145	27	104	6.38e-16	69.3
MS.gene97467.t1	AT2G21210	42.308	78	43	2	69	145	89	165	8.58e-16	70.5
MS.gene97467.t1	AT2G21220	44.928	69	35	1	74	142	39	104	2.24e-15	67.8
MS.gene97467.t1	AT3G43120	41.250	80	44	1	71	150	75	151	5.86e-15	68.2
MS.gene97467.t1	AT3G03850	47.761	67	33	2	76	141	26	91	6.51e-15	66.2
MS.gene97467.t1	AT4G38840	52.632	57	24	2	74	129	32	86	2.24e-14	65.1
MS.gene97467.t1	AT2G21200	52.632	57	24	2	74	129	20	74	2.30e-14	64.7
MS.gene97467.t1	AT4G34780	50.877	57	27	1	74	129	27	83	2.39e-14	65.1
MS.gene97467.t1	AT3G20220	42.466	73	37	2	68	137	37	107	2.77e-14	65.5
MS.gene97467.t1	AT4G34760	36.937	111	54	4	42	141	1	106	3.00e-14	65.1
MS.gene97467.t1	AT4G38825	44.000	75	39	2	68	141	16	88	3.32e-14	64.3
MS.gene97467.t1	AT3G12830	36.111	108	66	1	37	144	12	116	4.93e-14	65.1
MS.gene97467.t1	AT5G18080	47.368	76	31	4	55	129	10	77	5.95e-14	63.9
MS.gene97467.t1	AT5G20810	45.312	64	32	1	71	134	75	135	8.35e-14	65.5
MS.gene97467.t1	AT5G18020	49.123	57	26	2	74	129	23	77	1.52e-13	62.8
MS.gene97467.t1	AT5G20810	45.312	64	32	1	71	134	75	135	1.75e-13	65.1
MS.gene97467.t1	AT4G13790	46.774	62	31	2	69	129	19	79	2.10e-13	62.4
MS.gene97467.t1	AT3G03840	44.928	69	36	2	74	141	26	93	2.68e-13	62.4
MS.gene97467.t1	AT1G75580	35.455	110	53	4	45	141	3	107	2.81e-13	62.4
MS.gene97467.t1	AT5G18050	40.580	69	38	2	74	141	23	89	4.70e-13	61.6
MS.gene97467.t1	AT5G18060	44.737	76	33	4	55	129	10	77	5.18e-13	61.2
MS.gene97467.t1	AT3G03830	45.588	68	36	1	75	141	24	91	6.05e-13	61.2
MS.gene97467.t1	AT5G18030	40.789	76	34	3	55	129	10	75	1.07e-12	60.5
MS.gene97467.t1	AT4G38860	44.286	70	35	2	73	141	38	104	1.10e-12	60.8
MS.gene97467.t1	AT5G18010	44.737	76	33	4	55	129	10	77	1.26e-12	60.5
MS.gene97467.t1	AT1G19830	43.243	74	35	2	68	139	41	109	1.30e-12	60.8
MS.gene97467.t1	AT1G56150	41.096	73	40	1	75	147	38	107	3.34e-12	59.7
MS.gene97467.t1	AT5G66260	47.273	55	26	1	75	129	34	85	9.19e-12	58.5
MS.gene97467.t1	AT2G18010	41.892	74	39	2	56	129	30	99	1.12e-11	58.5
MS.gene97467.t1	AT5G10990	36.047	86	52	1	60	145	26	108	1.39e-11	58.9
MS.gene97467.t1	AT4G36110	48.214	56	26	1	74	129	39	91	1.41e-11	58.2
MS.gene97467.t1	AT2G28085	40.678	59	35	0	81	139	62	120	5.33e-11	57.0
MS.gene97467.t1	AT1G75590	41.892	74	37	2	62	135	32	99	6.60e-11	57.4

Find 47 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAAGAGGAAGAGATAAAT+AGG	0.176068	7.3:-60220899	MS.gene97467:CDS
CCTTTGGCACGCTGAATTTC+GGG	0.180419	7.3:+60220946	None:intergenic
CCCTTTGGCACGCTGAATTT+CGG	0.193021	7.3:+60220945	None:intergenic
AGCTCGGGAAGCGTCTCGTC+TGG	0.312851	7.3:-60221133	MS.gene97467:CDS
TACACAACCATTTGTCCCTT+TGG	0.333200	7.3:+60220930	None:intergenic
AAAGATGTTGAAGAGGAGTA+TGG	0.344907	7.3:-60220825	MS.gene97467:CDS
TGACCTGTTGAAAAGCTTCA+TGG	0.350356	7.3:+60220992	None:intergenic
TCTCGTCTGGGTTTCTAGAT+TGG	0.352336	7.3:-60221120	MS.gene97467:CDS
CGGCAATGAAACTTCTCAAA+TGG	0.352486	7.3:-60221037	MS.gene97467:CDS
TTGCATCAGGTTCCAGCAAT+TGG	0.352794	7.3:-60220734	MS.gene97467:CDS
TACGATACCATGTAGGTTTA+CGG	0.360176	7.3:-60220781	MS.gene97467:CDS
CATTTGAGAAGTTTCATTGC+CGG	0.372931	7.3:+60221038	None:intergenic
GGCAATGAAACTTCTCAAAT+GGG	0.383462	7.3:-60221036	MS.gene97467:CDS
GGAAGAGATAAATAGGGTTA+TGG	0.394223	7.3:-60220892	MS.gene97467:CDS
GAAAGAGGAAGAGATAAATA+GGG	0.396870	7.3:-60220898	MS.gene97467:CDS
GCTGGAACCTGATGCAATCC+AGG	0.450641	7.3:+60220740	None:intergenic
AGGTCAAGTTATACTCGGTT+AGG	0.455077	7.3:-60220975	MS.gene97467:CDS
TAAGCAATTCACTAAACAAT+GGG	0.459340	7.3:+60220847	None:intergenic
TTCATTGCCGGGGATTGAGC+AGG	0.474867	7.3:+60221050	None:intergenic
ATCAAGACCTGGATTGCATC+AGG	0.485980	7.3:-60220747	MS.gene97467:CDS
CAGCGTGCCAAAGGGACAAA+TGG	0.489582	7.3:-60220937	MS.gene97467:CDS
GTATCGTAATCCCACCTTGA+TGG	0.497196	7.3:+60220796	None:intergenic
GCTCGGGAAGCGTCTCGTCT+GGG	0.500414	7.3:-60221132	MS.gene97467:CDS
CCCGAAATTCAGCGTGCCAA+AGG	0.504666	7.3:-60220946	MS.gene97467:CDS
AGATCCACGGATTTAAGCTC+GGG	0.508966	7.3:-60221148	MS.gene97467:CDS
ATTTGAGAAGTTTCATTGCC+GGG	0.516399	7.3:+60221039	None:intergenic
TGGCACGCTGAATTTCGGGC+TGG	0.523303	7.3:+60220950	None:intergenic
GGGACAAATGGTTGTGTATG+TGG	0.525067	7.3:-60220925	MS.gene97467:CDS
CCGAAATTCAGCGTGCCAAA+GGG	0.533551	7.3:-60220945	MS.gene97467:CDS
TGTGTATGTGGGACAGAAAG+AGG	0.554416	7.3:-60220913	MS.gene97467:CDS
TTAAGCAATTCACTAAACAA+TGG	0.555508	7.3:+60220846	None:intergenic
ATTGCTTAAAGATGTTGAAG+AGG	0.557241	7.3:-60220832	MS.gene97467:CDS
AAGATCCACGGATTTAAGCT+CGG	0.560574	7.3:-60221149	MS.gene97467:CDS
GGGTGGTTAAAGTATATCAC+TGG	0.571494	7.3:+60220867	None:intergenic
TGATTGTCCTGCTCAATCCC+CGG	0.571774	7.3:-60221057	MS.gene97467:CDS
AGTTTGAACGGATCAAGACC+TGG	0.576336	7.3:-60220758	MS.gene97467:CDS
GTAGGTTTACGGAGTTTGAA+CGG	0.580984	7.3:-60220770	MS.gene97467:CDS
TCAACAGGTCAAGTTATACT+CGG	0.582354	7.3:-60220980	MS.gene97467:CDS
TCAAACTCCGTAAACCTACA+TGG	0.593678	7.3:+60220774	None:intergenic
GCAATTCACTAAACAATGGG+TGG	0.601342	7.3:+60220850	None:intergenic
TGGCATCCGTCGTTAGCTTG+CGG	0.604086	7.3:+60221012	None:intergenic
TTTGAGAAGTTTCATTGCCG+GGG	0.623296	7.3:+60221040	None:intergenic
GTGGGATTACGATACCATGT+AGG	0.627453	7.3:-60220788	MS.gene97467:CDS
GCTTCCCGAGCTTAAATCCG+TGG	0.636758	7.3:+60221144	None:intergenic
GGACAAATGGTTGTGTATGT+GGG	0.647999	7.3:-60220924	MS.gene97467:CDS
GCAATGAAACTTCTCAAATG+GGG	0.655342	7.3:-60221035	MS.gene97467:CDS
ATGGGGCCGCAAGCTAACGA+CGG	0.684381	7.3:-60221018	MS.gene97467:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TAAGCAATTCACTAAACAAT+GGG	+	chr7.3:60221036-60221055	None:intergenic	25.0%
!	TTAAGCAATTCACTAAACAA+TGG	+	chr7.3:60221037-60221056	None:intergenic	25.0%
	AGAAAGAGGAAGAGATAAAT+AGG	-	chr7.3:60220981-60221000	MS.gene97467:CDS	30.0%
	ATTGCTTAAAGATGTTGAAG+AGG	-	chr7.3:60221048-60221067	MS.gene97467:CDS	30.0%
	GAAAGAGGAAGAGATAAATA+GGG	-	chr7.3:60220982-60221001	MS.gene97467:CDS	30.0%
	AAAGATGTTGAAGAGGAGTA+TGG	-	chr7.3:60221055-60221074	MS.gene97467:CDS	35.0%
	ATTTGAGAAGTTTCATTGCC+GGG	+	chr7.3:60220844-60220863	None:intergenic	35.0%
	CATTTGAGAAGTTTCATTGC+CGG	+	chr7.3:60220845-60220864	None:intergenic	35.0%
	GCAATGAAACTTCTCAAATG+GGG	-	chr7.3:60220845-60220864	MS.gene97467:CDS	35.0%
	GGAAGAGATAAATAGGGTTA+TGG	-	chr7.3:60220988-60221007	MS.gene97467:CDS	35.0%
	GGCAATGAAACTTCTCAAAT+GGG	-	chr7.3:60220844-60220863	MS.gene97467:CDS	35.0%
	TACGATACCATGTAGGTTTA+CGG	-	chr7.3:60221099-60221118	MS.gene97467:CDS	35.0%
	TCAACAGGTCAAGTTATACT+CGG	-	chr7.3:60220900-60220919	MS.gene97467:CDS	35.0%
!	GATTGGTGTTTAAAAAGACG+CGG	-	chr7.3:60220777-60220796	MS.gene97467:CDS	35.0%
!!!	ATGGTTTTAACCATCAAGGT+GGG	-	chr7.3:60221074-60221093	MS.gene97467:CDS	35.0%
!!!	GAGTATGGTTTTAACCATCA+AGG	-	chr7.3:60221070-60221089	MS.gene97467:CDS	35.0%
!!!	TATGGTTTTAACCATCAAGG+TGG	-	chr7.3:60221073-60221092	MS.gene97467:CDS	35.0%
	AAGATCCACGGATTTAAGCT+CGG	-	chr7.3:60220731-60220750	MS.gene97467:CDS	40.0%
	AGGTCAAGTTATACTCGGTT+AGG	-	chr7.3:60220905-60220924	MS.gene97467:CDS	40.0%
	CGGCAATGAAACTTCTCAAA+TGG	-	chr7.3:60220843-60220862	MS.gene97467:CDS	40.0%
	GCAATTCACTAAACAATGGG+TGG	+	chr7.3:60221033-60221052	None:intergenic	40.0%
	GGACAAATGGTTGTGTATGT+GGG	-	chr7.3:60220956-60220975	MS.gene97467:CDS	40.0%
	GGGTGGTTAAAGTATATCAC+TGG	+	chr7.3:60221016-60221035	None:intergenic	40.0%
	GTAGGTTTACGGAGTTTGAA+CGG	-	chr7.3:60221110-60221129	MS.gene97467:CDS	40.0%
	TACACAACCATTTGTCCCTT+TGG	+	chr7.3:60220953-60220972	None:intergenic	40.0%
	TCAAACTCCGTAAACCTACA+TGG	+	chr7.3:60221109-60221128	None:intergenic	40.0%
	TGACCTGTTGAAAAGCTTCA+TGG	+	chr7.3:60220891-60220910	None:intergenic	40.0%
	TTTGAGAAGTTTCATTGCCG+GGG	+	chr7.3:60220843-60220862	None:intergenic	40.0%
!	ATGCCATGAAGCTTTTCAAC+AGG	-	chr7.3:60220885-60220904	MS.gene97467:CDS	40.0%
	AAAAAGACGCGGATTCGAAC+TGG	-	chr7.3:60220788-60220807	MS.gene97467:CDS	45.0%
	AGATCCACGGATTTAAGCTC+GGG	-	chr7.3:60220732-60220751	MS.gene97467:CDS	45.0%
	AGTTTGAACGGATCAAGACC+TGG	-	chr7.3:60221122-60221141	MS.gene97467:CDS	45.0%
	ATCAAGACCTGGATTGCATC+AGG	-	chr7.3:60221133-60221152	MS.gene97467:CDS	45.0%
	GGGACAAATGGTTGTGTATG+TGG	-	chr7.3:60220955-60220974	MS.gene97467:CDS	45.0%
	GTATCGTAATCCCACCTTGA+TGG	+	chr7.3:60221087-60221106	None:intergenic	45.0%
	GTGGGATTACGATACCATGT+AGG	-	chr7.3:60221092-60221111	MS.gene97467:CDS	45.0%
	TGTGTATGTGGGACAGAAAG+AGG	-	chr7.3:60220967-60220986	MS.gene97467:CDS	45.0%
	TTGCATCAGGTTCCAGCAAT+TGG	-	chr7.3:60221146-60221165	MS.gene97467:CDS	45.0%
!	TCTCGTCTGGGTTTCTAGAT+TGG	-	chr7.3:60220760-60220779	MS.gene97467:CDS	45.0%
	CCCTTTGGCACGCTGAATTT+CGG	+	chr7.3:60220938-60220957	None:intergenic	50.0%
	CCGAAATTCAGCGTGCCAAA+GGG	-	chr7.3:60220935-60220954	MS.gene97467:CDS	50.0%
	CCTTTGGCACGCTGAATTTC+GGG	+	chr7.3:60220937-60220956	None:intergenic	50.0%
	TGATTGTCCTGCTCAATCCC+CGG	-	chr7.3:60220823-60220842	MS.gene97467:CDS	50.0%
	CAGCGTGCCAAAGGGACAAA+TGG	-	chr7.3:60220943-60220962	MS.gene97467:CDS	55.0%
	CCCGAAATTCAGCGTGCCAA+AGG	-	chr7.3:60220934-60220953	MS.gene97467:CDS	55.0%
	GCTGGAACCTGATGCAATCC+AGG	+	chr7.3:60221143-60221162	None:intergenic	55.0%
	GCTTCCCGAGCTTAAATCCG+TGG	+	chr7.3:60220739-60220758	None:intergenic	55.0%
	TTCATTGCCGGGGATTGAGC+AGG	+	chr7.3:60220833-60220852	None:intergenic	55.0%
!	TGGCATCCGTCGTTAGCTTG+CGG	+	chr7.3:60220871-60220890	None:intergenic	55.0%
	ATGGGGCCGCAAGCTAACGA+CGG	-	chr7.3:60220862-60220881	MS.gene97467:CDS	60.0%
	TGGCACGCTGAATTTCGGGC+TGG	+	chr7.3:60220933-60220952	None:intergenic	60.0%
!!	AGCTCGGGAAGCGTCTCGTC+TGG	-	chr7.3:60220747-60220766	MS.gene97467:CDS	65.0%
!!	GCTCGGGAAGCGTCTCGTCT+GGG	-	chr7.3:60220748-60220767	MS.gene97467:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	60220725	60221177	60220725	ID=MS.gene97467
chr7.3	mRNA	60220725	60221177	60220725	ID=MS.gene97467.t1;Parent=MS.gene97467
chr7.3	exon	60220725	60221177	60220725	ID=MS.gene97467.t1.exon1;Parent=MS.gene97467.t1
chr7.3	CDS	60220725	60221177	60220725	ID=cds.MS.gene97467.t1;Parent=MS.gene97467.t1

Gene Sequence

>MS.gene97467

ATGGCAAAGATCCACGGATTTAAGCTCGGGAAGCGTCTCGTCTGGGTTTCTAGATTGGTGTTTAAAAAGACGCGGATTCGAACTGGATATCATCGCTTTGATTGTCCTGCTCAATCCCCGGCAATGAAACTTCTCAAATGGGGCCGCAAGCTAACGACGGATGCCATGAAGCTTTTCAACAGGTCAAGTTATACTCGGTTAGGATCCAGCCCGAAATTCAGCGTGCCAAAGGGACAAATGGTTGTGTATGTGGGACAGAAAGAGGAAGAGATAAATAGGGTTATGGTGCCAGTGATATACTTTAACCACCCATTGTTTAGTGAATTGCTTAAAGATGTTGAAGAGGAGTATGGTTTTAACCATCAAGGTGGGATTACGATACCATGTAGGTTTACGGAGTTTGAACGGATCAAGACCTGGATTGCATCAGGTTCCAGCAATTGGACCAGTTGA

Protein sequence

>MS.gene97467.t1

MAKIHGFKLGKRLVWVSRLVFKKTRIRTGYHRFDCPAQSPAMKLLKWGRKLTTDAMKLFNRSSYTRLGSSPKFSVPKGQMVVYVGQKEEEINRVMVPVIYFNHPLFSELLKDVEEEYGFNHQGGITIPCRFTEFERIKTWIASGSSNWTS