AlfalfaGEDB | MS.gene99124

M. sativa cultivar XinJiangDaYe / MS.gene99124

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF12854.10 PF13041.9 PF20431.1

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99124.t1	PNY10183.1	89.8	352	36	0	1	352	149	500	5.30E-154	553.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99124.t1	Q680Z7	59.5	353	141	2	1	352	152	503	1.5e-101	370.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99124.t1	A0A2K3P4K2	89.8	352	36	0	1	352	149	500	3.9e-154	553.9

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene99124	0.833485	5.19E-56	-1.69E-46
MS.gene049825	MS.gene99124	0.803806	2.92E-49	-1.69E-46
MS.gene050026	MS.gene99124	0.815603	8.49E-52	-1.69E-46
MS.gene051719	MS.gene99124	0.826911	2.09E-54	-1.69E-46
MS.gene051720	MS.gene99124	0.820127	8.08E-53	-1.69E-46
MS.gene052519	MS.gene99124	0.810664	1.03E-50	-1.69E-46
MS.gene052582	MS.gene99124	0.836254	1.04E-56	-1.69E-46
MS.gene052584	MS.gene99124	0.831492	1.62E-55	-1.69E-46
MS.gene053226	MS.gene99124	0.833076	6.56E-56	-1.69E-46
MS.gene05364	MS.gene99124	0.804045	2.61E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene013566	MS.gene99124	PPI
MS.gene057128	MS.gene99124	PPI
MS.gene06683	MS.gene99124	PPI
MS.gene06894	MS.gene99124	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99124.t1	MTR_4g023510	94.318	352	20	0	1	352	143	494	0.0	695
MS.gene99124.t1	MTR_4g084680	74.062	320	35	2	33	352	1	272	2.68e-166	465
MS.gene99124.t1	MTR_3g109380	41.026	351	202	4	2	352	141	486	1.81e-94	291
MS.gene99124.t1	MTR_8g027150	39.886	351	208	3	2	352	109	456	1.15e-90	281
MS.gene99124.t1	MTR_8g027150	22.258	310	200	9	28	305	6	306	1.20e-15	78.2
MS.gene99124.t1	MTR_7g011100	40.751	346	201	3	7	352	174	515	8.91e-90	279
MS.gene99124.t1	MTR_1g083890	39.429	350	208	3	1	350	137	482	4.02e-88	273
MS.gene99124.t1	MTR_7g076707	40.401	349	203	4	4	352	209	552	4.22e-87	273
MS.gene99124.t1	MTR_7g076707	25.839	298	154	6	1	267	105	366	1.63e-21	95.9
MS.gene99124.t1	MTR_7g076707	27.731	238	160	6	33	267	6	234	3.16e-17	83.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99124.t1	AT1G09220	56.091	353	153	2	1	352	152	503	1.56e-149	431
MS.gene99124.t1	AT2G20540	40.634	347	201	4	6	352	113	454	1.01e-95	293
MS.gene99124.t1	AT2G20540	27.160	243	122	9	91	295	69	294	2.30e-17	83.6
MS.gene99124.t1	AT2G20540	40.634	347	201	4	6	352	143	484	4.12e-95	293
MS.gene99124.t1	AT2G20540	24.643	280	160	8	54	295	58	324	4.37e-20	91.7
MS.gene99124.t1	AT5G48910	42.350	366	190	8	2	352	158	517	3.32e-91	286
MS.gene99124.t1	AT5G48910	28.873	284	151	6	54	291	75	353	2.00e-29	119
MS.gene99124.t1	AT5G56310	40.510	353	203	5	2	352	146	493	7.12e-89	277
MS.gene99124.t1	AT5G06540	39.316	351	208	4	2	352	147	492	4.09e-87	275
MS.gene99124.t1	AT5G15300	39.601	351	203	5	6	352	177	522	1.35e-84	266

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Find 97 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACTGGAGATGGAGAA+GGG	0.572276	4.3:-74769754	MS.gene99124:CDS
AAATGGAAAAGGCTGGTGTG+CGG	0.687117	4.3:-74770065	MS.gene99124:CDS
AACAAAGCCAAAGCTTTAAA+AGG	0.228473	4.3:+74770408	None:intergenic
AACATCATTTGCGACCTCAC+AGG	0.513350	4.3:+74769846	None:intergenic
AAGAAATGTCTTTAAACTTC+CGG	0.257038	4.3:-74769641	MS.gene99124:CDS
AATCATGTCTTTGTGCAAAC+TGG	0.359218	4.3:-74770648	MS.gene99124:CDS
ACAATGTTGAAATTGGTAAG+AGG	0.502454	4.3:-74769786	MS.gene99124:CDS
ACAGTGGATTGGTTGAGGAG+GGG	0.586548	4.3:-74769999	MS.gene99124:CDS
ACATCATTTGCGACCTCACA+GGG	0.554423	4.3:+74769847	None:intergenic
ACCGAATTCCTCCTCCAGTC+AGG	0.490353	4.3:+74770159	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAATGTTGAAATTGGTAAG+AGG	-	chr4.3:74770498-74770517	MS.gene99124:CDS	30.0%
AGAAATGTCTTTAAACTTCC+GGG	-	chr4.3:74770644-74770663	MS.gene99124:CDS	30.0%
ATGTCTGGTTATGCTATATT+TGG	-	chr4.3:74770164-74770183	MS.gene99124:CDS	30.0%
CAATGTTGAAATTGGTAAGA+GGG	-	chr4.3:74770499-74770518	MS.gene99124:CDS	30.0%
CTTCATATGTACTCAAATTG+GGG	-	chr4.3:74769663-74769682	MS.gene99124:CDS	30.0%
GCTTCATATGTACTCAAATT+GGG	-	chr4.3:74769662-74769681	MS.gene99124:CDS	30.0%
GGTAAAATGGTGAATGATTA+CGG	-	chr4.3:74770320-74770339	MS.gene99124:CDS	30.0%
GTTAAAAGAGTAACCTCATT+AGG	+	chr4.3:74769933-74769952	None:intergenic	30.0%
TGCTTCATATGTACTCAAAT+TGG	-	chr4.3:74769661-74769680	MS.gene99124:CDS	30.0%
TGTCTGGTTATGCTATATTT+GGG	-	chr4.3:74770165-74770184	MS.gene99124:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	74769624	74770682	74769624	ID=MS.gene99124
chr4.3	mRNA	74769624	74770682	74769624	ID=MS.gene99124.t1;Parent=MS.gene99124
chr4.3	exon	74769624	74770682	74769624	ID=MS.gene99124.t1.exon1;Parent=MS.gene99124.t1
chr4.3	CDS	74769624	74770682	74769624	ID=cds.MS.gene99124.t1;Parent=MS.gene99124.t1

Gene Sequence

>MS.gene99124

ATGGGTTTTGAGAATCATGTCTTTGTGCAAACTGGGTTGCTTCATATGTACTCAAATTGGGGTTTTTTGGTTGATGCAGCTCAGGTGTTCGGTGAAATGCCGGAGAGAAATACGGTTACTTGGAATGTTTTTATTAGTGGTTTGATTAAATGGGGTCAGCTTGAGTTTGCGAGGTCGGTTTTTGATCAAATGGTGGTTCGGAGTGTGGTGTCGTGGACTCTTGTGATTGATGGGTATACGAGGATGAATAAGCCTTTTAAAGCTTTGGCTTTGTTTAGGAAAATGATTGAGGTTGATGGGATAGAGCCTAATGAGGTTACTCTTTTAACTGTTTTTCCTGCTATTGCTAATCTTGGGAATGTTGAGGTGTGTGAGTCGGTTCATGGGTATGTGGAGAAGAGAGGGTTTAATGCGGTTGATATAAGGATTTTGAATGCGTTGATTGATTTGTATGCTAAGTGTGGATGTATAGAGAGTGCGAGTAGATTGTTTTCGGAGATGCCTGACTGGAGGAGGAATTCGGTGTCGTGGAATACGGTTATGTCTGGTTATGCTATATTTGGGATGGTGAGGGAGGTTGTGGATACTTTTGAAAAAATGGAAAAGGCTGGTGTGCGGCCGAATCATGTGGCATTCCTCAGTATTTTGAGTGCTTGTAGTCACAGTGGATTGGTTGAGGAGGGGCTTGAGTTTTTCGGTAAAATGGTGAATGATTACGGACTTGTGCCGGATGTCAAGCACTATGGTTGTGTGATAGATATGCTTGGGAGAGCAGGGAGGTTAGAAGAAGCTGAGCAGGTTGCTTTGCAGGTTCCCTGTGAGGTCGCAAATGATGTTATTTGGAGGACGCTTCTGGGTGCTTGTAGCATTCATGACAATGTTGAAATTGGTAAGAGGGTGACCAAGAAAATACTGGAGATGGAGAAGGGACATGGTGGTGATTATGTTCTAATGTCCAATATTTTTGCTGGTGTTGGGAGATTCAAAGATGTTGAGAGGTTAAGGGAAATGATAGATAAAAGAAATGTCTTTAAACTTCCGGGCTACAGTTTTGTCTAA

Protein sequence

>MS.gene99124.t1

MGFENHVFVQTGLLHMYSNWGFLVDAAQVFGEMPERNTVTWNVFISGLIKWGQLEFARSVFDQMVVRSVVSWTLVIDGYTRMNKPFKALALFRKMIEVDGIEPNEVTLLTVFPAIANLGNVEVCESVHGYVEKRGFNAVDIRILNALIDLYAKCGCIESASRLFSEMPDWRRNSVSWNTVMSGYAIFGMVREVVDTFEKMEKAGVRPNHVAFLSILSACSHSGLVEEGLEFFGKMVNDYGLVPDVKHYGCVIDMLGRAGRLEEAEQVALQVPCEVANDVIWRTLLGACSIHDNVEIGKRVTKKILEMEKGHGGDYVLMSNIFAGVGRFKDVERLREMIDKRNVFKLPGYSFV