AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022620.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022620.01.T01 MTR_4g093660 98.824 85 1 0 1 85 1 85 4.18e-58 172
MsG0480022620.01.T01 MTR_2g009660 85.882 85 12 0 1 85 63 147 2.66e-50 155
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022620.01.T01 AT4G33640 80.769 78 15 0 1 78 1 78 1.13e-41 132
MsG0480022620.01.T01 AT4G33640 80.769 78 15 0 1 78 67 144 3.48e-41 132

Find 20 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCATTGTGAAGGTCACATT+TGG 0.398089 4:+74787838 MsG0480022620.01.T01:intron
CCTGCTGCTGCTAACTGATT+CGG 0.442731 4:+74788012 MsG0480022620.01.T02:CDS
GAGTGATTTCCACATTATCA+TGG 0.446001 4:-74787978 None:intergenic
GAACACTCTTTCACGATGAT+CGG 0.491735 4:+74787860 MsG0480022620.01.T01:CDS
AAGAGGAGGTTGAAAAGCTG+AGG 0.496528 4:+74787107 MsG0480022620.01.T01:CDS
CCGAATCAGTTAGCAGCAGC+AGG 0.502169 4:-74788012 None:intergenic
CAGTTAGCAGCAGCAGGAGC+AGG 0.504867 4:-74788006 None:intergenic
CTTGGAAATGTTCAAGCAGA+TGG 0.516658 4:+74787145 MsG0480022620.01.T01:CDS
AGGGAAGAAATCAAGAGGCT+TGG 0.527446 4:+74787127 MsG0480022620.01.T01:CDS
GCTAACTGATTCGGTTGTGA+AGG 0.539593 4:+74788021 MsG0480022620.01.T02:CDS
TGTGAAGATGAACGTAGAAG+AGG 0.563453 4:+74787090 None:intergenic
GAAGATGAACGTAGAAGAGG+AGG 0.565552 4:+74787093 None:intergenic
AGAGGAGGTTGAAAAGCTGA+GGG 0.572997 4:+74787108 MsG0480022620.01.T01:CDS
AATATATTTGAAGCACTCGT+CGG 0.601303 4:+74787889 MsG0480022620.01.T01:CDS
TCAAGCAGATGGTTCTTGCA+AGG 0.604584 4:+74787156 MsG0480022620.01.T01:CDS
CTAACTGATTCGGTTGTGAA+GGG 0.617075 4:+74788022 MsG0480022620.01.T02:CDS
AGCTGAGGGAAGAAATCAAG+AGG 0.626324 4:+74787122 MsG0480022620.01.T01:CDS
GACGGTGAACTACTGTTGCA+AGG 0.632747 4:+74787952 MsG0480022620.01.T01:CDS
GCAAGGAGTCCATGATAATG+TGG 0.676630 4:+74787969 MsG0480022620.01.T01:CDS
CGTAAAGTAGTTGCATACGA+CGG 0.749247 4:+74787934 MsG0480022620.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATAAACAAATTAATGTAT+AGG - Chr4:74787468-74787487 None:intergenic 10.0%
!! ATAATTGTAATTAAATGTGT+TGG + Chr4:74787624-74787643 MsG0480022620.01.T01:intron 15.0%
!!! AATTTGTTTATTATAAACCA+TGG + Chr4:74787475-74787494 MsG0480022620.01.T01:intron 15.0%
!! GAATCAAGTTCTTAATTAAA+AGG - Chr4:74787262-74787281 None:intergenic 20.0%
!! TTTCATATGATATTTCGTAA+TGG - Chr4:74787395-74787414 None:intergenic 20.0%
!!! AAGTATTGATATGATGTTTA+GGG - Chr4:74787602-74787621 None:intergenic 20.0%
! AAATATGTAATGCATTGTGA+AGG + Chr4:74787828-74787847 MsG0480022620.01.T01:intron 25.0%
! ATGCATCATGAAAATGTATA+TGG - Chr4:74787224-74787243 None:intergenic 25.0%
! CATTCCATATAAAACTGATA+TGG + Chr4:74787521-74787540 MsG0480022620.01.T01:intron 25.0%
! TAATGTATAGGTAGATACAT+TGG - Chr4:74787456-74787475 None:intergenic 25.0%
! TCAGATCTAGATATATTGAT+GGG + Chr4:74787280-74787299 MsG0480022620.01.T01:intron 25.0%
! TTCAGATCTAGATATATTGA+TGG + Chr4:74787279-74787298 MsG0480022620.01.T01:intron 25.0%
! TTGTTTATTATAAACCATGG+AGG + Chr4:74787478-74787497 MsG0480022620.01.T01:intron 25.0%
!!! AATTTGCTGAAATAGTACTA+TGG + Chr4:74787684-74787703 MsG0480022620.01.T01:intron 25.0%
!!! CAAGTATTGATATGATGTTT+AGG - Chr4:74787603-74787622 None:intergenic 25.0%
!!! CATATCAGTTTTATATGGAA+TGG - Chr4:74787523-74787542 None:intergenic 25.0%
TATAGTGAGTGATGGTTAAT+TGG + Chr4:74787734-74787753 MsG0480022620.01.T01:intron 30.0%
TTCTTGCAAGGTATAACAAA+AGG + Chr4:74787168-74787187 MsG0480022620.01.T01:intron 30.0%
! AATATATTTGAAGCACTCGT+CGG + Chr4:74787889-74787908 MsG0480022620.01.T01:CDS 30.0%
! CTATATCTTCAGTTGATTCA+TGG - Chr4:74787719-74787738 None:intergenic 30.0%
! GAAAATGTATATGGTTTGCA+AGG - Chr4:74787215-74787234 None:intergenic 30.0%
! TGATGGGTATTTATTGAATG+TGG + Chr4:74787296-74787315 MsG0480022620.01.T01:intron 30.0%
! TGGTCACTTTCTTATTCTTA+TGG + Chr4:74787316-74787335 MsG0480022620.01.T01:intron 30.0%
!! CTAGCCATATCAGTTTTATA+TGG - Chr4:74787528-74787547 None:intergenic 30.0%
ACTGAAGATATAGTGAGTGA+TGG + Chr4:74787726-74787745 MsG0480022620.01.T01:intron 35.0%
GAGTGATTTCCACATTATCA+TGG - Chr4:74787981-74788000 None:intergenic 35.0%
GTTAATGCGCATATGTGAAT+TGG + Chr4:74787764-74787783 MsG0480022620.01.T01:intron 35.0%
TCTTATGGATCTAGATCACA+AGG + Chr4:74787331-74787350 MsG0480022620.01.T01:intron 35.0%
CACAAGGTCATTCACAACAT+TGG + Chr4:74787347-74787366 MsG0480022620.01.T01:intron 40.0%
CGTAAAGTAGTTGCATACGA+CGG + Chr4:74787934-74787953 MsG0480022620.01.T01:CDS 40.0%
CTTGGAAATGTTCAAGCAGA+TGG + Chr4:74787145-74787164 MsG0480022620.01.T01:CDS 40.0%
GAACACTCTTTCACGATGAT+CGG + Chr4:74787860-74787879 MsG0480022620.01.T01:CDS 40.0%
TGCATTGTGAAGGTCACATT+TGG + Chr4:74787838-74787857 MsG0480022620.01.T01:intron 40.0%
!! CTAACTGATTCGGTTGTGAA+GGG + Chr4:74788022-74788041 MsG0480022620.01.T02:CDS 40.0%
AAGAGGAGGTTGAAAAGCTG+AGG + Chr4:74787107-74787126 MsG0480022620.01.T01:CDS 45.0%
AGAGGAGGTTGAAAAGCTGA+GGG + Chr4:74787108-74787127 MsG0480022620.01.T01:CDS 45.0%
AGCTGAGGGAAGAAATCAAG+AGG + Chr4:74787122-74787141 MsG0480022620.01.T01:CDS 45.0%
AGGGAAGAAATCAAGAGGCT+TGG + Chr4:74787127-74787146 MsG0480022620.01.T01:CDS 45.0%
TCAAGCAGATGGTTCTTGCA+AGG + Chr4:74787156-74787175 MsG0480022620.01.T01:CDS 45.0%
! GCAAGGAGTCCATGATAATG+TGG + Chr4:74787969-74787988 MsG0480022620.01.T01:CDS 45.0%
!! GCTAACTGATTCGGTTGTGA+AGG + Chr4:74788021-74788040 MsG0480022620.01.T02:CDS 45.0%
CCTGCTGCTGCTAACTGATT+CGG + Chr4:74788012-74788031 MsG0480022620.01.T02:CDS 50.0%
CGCATGATATCTCTCCTCCA+TGG - Chr4:74787495-74787514 None:intergenic 50.0%
GACGGTGAACTACTGTTGCA+AGG + Chr4:74787952-74787971 MsG0480022620.01.T01:CDS 50.0%
CCGAATCAGTTAGCAGCAGC+AGG - Chr4:74788015-74788034 None:intergenic 55.0%
CAGTTAGCAGCAGCAGGAGC+AGG - Chr4:74788009-74788028 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 74787097 74788051 74787097 ID=MsG0480022620.01;Name=MsG0480022620.01
Chr4 mRNA 74787097 74788029 74787097 ID=MsG0480022620.01.T01;Parent=MsG0480022620.01;Name=MsG0480022620.01.T01;_AED=0.44;_eAED=0.45;_QI=0|0|0|1|1|1|2|0|86
Chr4 exon 74787097 74787177 74787097 ID=MsG0480022620.01.T01:exon:5526;Parent=MsG0480022620.01.T01
Chr4 exon 74787850 74788029 74787850 ID=MsG0480022620.01.T01:exon:5527;Parent=MsG0480022620.01.T01
Chr4 CDS 74787097 74787177 74787097 ID=MsG0480022620.01.T01:cds;Parent=MsG0480022620.01.T01
Chr4 CDS 74787850 74788029 74787850 ID=MsG0480022620.01.T01:cds;Parent=MsG0480022620.01.T01
Chr4 mRNA 74787980 74788051 74787980 ID=MsG0480022620.01.T02;Parent=MsG0480022620.01;Name=MsG0480022620.01.T02;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|23
Chr4 exon 74787980 74788051 74787980 ID=MsG0480022620.01.T02:exon:5528;Parent=MsG0480022620.01.T02
Chr4 CDS 74787980 74788051 74787980 ID=MsG0480022620.01.T02:cds;Parent=MsG0480022620.01.T02
Gene Sequence

>MsG0480022620.01.T01

ATGAACGTAGAAGAGGAGGTTGAAAAGCTGAGGGAAGAAATCAAGAGGCTTGGAAATGTTCAAGCAGATGGTTCTTGCAAGGTCACATTTGGAACACTCTTTCACGATGATCGGTGCGCAAATATATTTGAAGCACTCGTCGGTACACTAAGAGCTGCTAAAAAGCGTAAAGTAGTTGCATACGACGGTGAACTACTGTTGCAAGGAGTCCATGATAATGTGGAAATCACTCTTAATCCTGCTCCTGCTGCTGCTAACTGA

>MsG0480022620.01.T02

ATGATAATGTGGAAATCACTCTTAATCCTGCTCCTGCTGCTGCTAACTGATTCGGTTGTGAAGGGTCATTGA

Protein sequence

>MsG0480022620.01.T01

MNVEEEVEKLREEIKRLGNVQADGSCKVTFGTLFHDDRCANIFEALVGTLRAAKKRKVVAYDGELLLQGVHDNVEITLNPAPAAAN*

>MsG0480022620.01.T02

MIMWKSLLILLLLLLTDSVVKGH*