AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016985.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016985.01.T01 MTR_3g098730 97.196 107 3 0 1 107 431 537 3.52e-70 219
MsG0380016985.01.T01 MTR_7g076220 42.991 107 61 0 1 107 437 543 6.03e-22 89.4
MsG0380016985.01.T01 MTR_8g032570 42.056 107 62 0 1 107 438 544 1.62e-21 88.2
MsG0380016985.01.T01 MTR_8g032570 42.056 107 62 0 1 107 461 567 1.93e-21 87.8
MsG0380016985.01.T01 MTR_4g007850 35.922 103 65 1 1 102 394 496 8.39e-13 63.2
MsG0380016985.01.T01 MTR_4g091320 33.654 104 68 1 1 103 449 552 1.61e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016985.01.T01 AT5G50900 57.944 107 45 0 1 107 432 538 1.50e-35 127
MsG0380016985.01.T01 AT2G45720 46.729 107 57 0 1 107 430 536 7.43e-24 94.7
MsG0380016985.01.T01 AT2G45720 46.729 107 57 0 1 107 430 536 7.43e-24 94.7
MsG0380016985.01.T01 AT2G45720 46.729 107 57 0 1 107 430 536 7.43e-24 94.7
MsG0380016985.01.T01 AT2G45720 46.729 107 57 0 1 107 430 536 7.43e-24 94.7
MsG0380016985.01.T01 AT2G45720 46.729 107 57 0 1 107 430 536 7.43e-24 94.7
MsG0380016985.01.T01 AT1G01830 35.185 108 69 1 1 107 451 558 1.22e-13 65.9
MsG0380016985.01.T01 AT1G01830 35.185 108 69 1 1 107 451 558 1.22e-13 65.9
MsG0380016985.01.T01 AT1G01830 35.185 108 69 1 1 107 451 558 1.22e-13 65.9
MsG0380016985.01.T01 AT1G61350 31.858 113 71 1 1 107 441 553 1.38e-12 62.8

Find 35 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACCCTGCTTTGGTTAATT+TGG 0.181771 3:-90312758 MsG0380016985.01.T01:CDS
GCTTGTTGAGTTGGATATTC+CGG 0.273134 3:-90312638 MsG0380016985.01.T01:CDS
GAAATACCCTGTTTCGGTTC+TGG 0.283357 3:-90312731 MsG0380016985.01.T01:CDS
CTTTCAATAGAAGGATCTTT+AGG 0.301417 3:-90312819 MsG0380016985.01.T01:CDS
TGCAGCACCCTTTCAATAGA+AGG 0.329460 3:-90312828 MsG0380016985.01.T01:CDS
CTTGTTGAGTTGGATATTCC+GGG 0.366525 3:-90312637 MsG0380016985.01.T01:CDS
TAACCAAAGCAGGGTTCAAT+AGG 0.373568 3:+90312764 None:intergenic
AGTGAAACCAGAACCGAAAC+AGG 0.386932 3:+90312724 None:intergenic
TCACCTATTGAACCCTGCTT+TGG 0.400666 3:-90312767 MsG0380016985.01.T01:CDS
CGGAAACAAATGGTGGCTGC+TGG 0.406988 3:-90312682 MsG0380016985.01.T01:CDS
GGATAAGAAATACCCTGTTT+CGG 0.411451 3:-90312737 MsG0380016985.01.T01:CDS
TCCTTCTTTGATTAAGATGT+TGG 0.421703 3:-90312905 MsG0380016985.01.T01:CDS
TTATCCAAATTAACCAAAGC+AGG 0.430725 3:+90312754 None:intergenic
ATCTGCTGCAATGGCGTTGT+CGG 0.448484 3:-90312857 MsG0380016985.01.T01:CDS
TAAAGATCCTTCTATTGAAA+GGG 0.462836 3:+90312821 None:intergenic
CTAAAGATCCTTCTATTGAA+AGG 0.463381 3:+90312820 None:intergenic
TACGCAGAAGCTTGTTGAGT+TGG 0.472425 3:-90312647 MsG0380016985.01.T01:CDS
TCTTTGATTAAGATGTTGGA+TGG 0.483116 3:-90312901 MsG0380016985.01.T01:CDS
TCCAACATCTTAATCAAAGA+AGG 0.499119 3:+90312904 None:intergenic
GGAAACAAATGGTGGCTGCT+GGG 0.501374 3:-90312681 MsG0380016985.01.T01:CDS
ATCTAACATTTCTTTGAACC+CGG 0.508397 3:+90312619 None:intergenic
ATTAAGATGTTGGATGGGAA+AGG 0.520569 3:-90312895 MsG0380016985.01.T01:CDS
GAAGACTTGTCGGAAACAAA+TGG 0.525091 3:-90312692 MsG0380016985.01.T01:CDS
GGATCTTTAGGAAGGATGAG+AGG 0.530549 3:-90312807 MsG0380016985.01.T01:CDS
CTTTGATTAAGATGTTGGAT+GGG 0.539664 3:-90312900 MsG0380016985.01.T01:CDS
GATCTTTAGGAAGGATGAGA+GGG 0.541790 3:-90312806 MsG0380016985.01.T01:CDS
GAAACAAATGGTGGCTGCTG+GGG 0.546643 3:-90312680 MsG0380016985.01.T01:CDS
CAATAGAAGGATCTTTAGGA+AGG 0.547514 3:-90312815 MsG0380016985.01.T01:CDS
ATCTTTAGGAAGGATGAGAG+GGG 0.554644 3:-90312805 MsG0380016985.01.T01:CDS
GGAGAAAGAATCTGCTGCAA+TGG 0.567130 3:-90312866 MsG0380016985.01.T01:CDS
TATCCAAATTAACCAAAGCA+GGG 0.575946 3:+90312755 None:intergenic
GATGTTGGATGGGAAAGGCG+TGG 0.603804 3:-90312890 MsG0380016985.01.T01:CDS
GTTGGATGGGAAAGGCGTGG+AGG 0.641910 3:-90312887 MsG0380016985.01.T01:CDS
GACTTGTCGGAAACAAATGG+TGG 0.684138 3:-90312689 MsG0380016985.01.T01:CDS
GTGAAACCAGAACCGAAACA+GGG 0.684741 3:+90312725 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAGATCCTTCTATTGAAA+GGG + Chr3:90312728-90312747 None:intergenic 25.0%
CTAAAGATCCTTCTATTGAA+AGG + Chr3:90312729-90312748 None:intergenic 30.0%
CTTTCAATAGAAGGATCTTT+AGG - Chr3:90312727-90312746 MsG0380016985.01.T01:CDS 30.0%
CTTTGATTAAGATGTTGGAT+GGG - Chr3:90312646-90312665 MsG0380016985.01.T01:CDS 30.0%
TATCCAAATTAACCAAAGCA+GGG + Chr3:90312794-90312813 None:intergenic 30.0%
TCCAACATCTTAATCAAAGA+AGG + Chr3:90312645-90312664 None:intergenic 30.0%
TCCTTCTTTGATTAAGATGT+TGG - Chr3:90312641-90312660 MsG0380016985.01.T01:CDS 30.0%
TCTTTGATTAAGATGTTGGA+TGG - Chr3:90312645-90312664 MsG0380016985.01.T01:CDS 30.0%
TTATCCAAATTAACCAAAGC+AGG + Chr3:90312795-90312814 None:intergenic 30.0%
ATTAAGATGTTGGATGGGAA+AGG - Chr3:90312651-90312670 MsG0380016985.01.T01:CDS 35.0%
GGATAAGAAATACCCTGTTT+CGG - Chr3:90312809-90312828 MsG0380016985.01.T01:CDS 35.0%
! CAATAGAAGGATCTTTAGGA+AGG - Chr3:90312731-90312750 MsG0380016985.01.T01:CDS 35.0%
! TTTTGCATTCGAAGACTTGT+CGG - Chr3:90312844-90312863 MsG0380016985.01.T01:CDS 35.0%
GAACCCTGCTTTGGTTAATT+TGG - Chr3:90312788-90312807 MsG0380016985.01.T01:CDS 40.0%
GAAGACTTGTCGGAAACAAA+TGG - Chr3:90312854-90312873 MsG0380016985.01.T01:CDS 40.0%
TAACCAAAGCAGGGTTCAAT+AGG + Chr3:90312785-90312804 None:intergenic 40.0%
! ATCTTTAGGAAGGATGAGAG+GGG - Chr3:90312741-90312760 MsG0380016985.01.T01:CDS 40.0%
! GATCTTTAGGAAGGATGAGA+GGG - Chr3:90312740-90312759 MsG0380016985.01.T01:CDS 40.0%
!! CTTGTTGAGTTGGATATTCC+GGG - Chr3:90312909-90312928 MsG0380016985.01.T01:CDS 40.0%
!! GCTTGTTGAGTTGGATATTC+CGG - Chr3:90312908-90312927 MsG0380016985.01.T01:CDS 40.0%
AGTGAAACCAGAACCGAAAC+AGG + Chr3:90312825-90312844 None:intergenic 45.0%
GAAATACCCTGTTTCGGTTC+TGG - Chr3:90312815-90312834 MsG0380016985.01.T01:CDS 45.0%
GACTTGTCGGAAACAAATGG+TGG - Chr3:90312857-90312876 MsG0380016985.01.T01:CDS 45.0%
GGAGAAAGAATCTGCTGCAA+TGG - Chr3:90312680-90312699 MsG0380016985.01.T01:CDS 45.0%
GTGAAACCAGAACCGAAACA+GGG + Chr3:90312824-90312843 None:intergenic 45.0%
TCACCTATTGAACCCTGCTT+TGG - Chr3:90312779-90312798 MsG0380016985.01.T01:CDS 45.0%
TGCAGCACCCTTTCAATAGA+AGG - Chr3:90312718-90312737 MsG0380016985.01.T01:CDS 45.0%
! GGATCTTTAGGAAGGATGAG+AGG - Chr3:90312739-90312758 MsG0380016985.01.T01:CDS 45.0%
!! TACGCAGAAGCTTGTTGAGT+TGG - Chr3:90312899-90312918 MsG0380016985.01.T01:CDS 45.0%
ATCTGCTGCAATGGCGTTGT+CGG - Chr3:90312689-90312708 MsG0380016985.01.T01:CDS 50.0%
GAAACAAATGGTGGCTGCTG+GGG - Chr3:90312866-90312885 MsG0380016985.01.T01:CDS 50.0%
GGAAACAAATGGTGGCTGCT+GGG - Chr3:90312865-90312884 MsG0380016985.01.T01:CDS 50.0%
CGGAAACAAATGGTGGCTGC+TGG - Chr3:90312864-90312883 MsG0380016985.01.T01:CDS 55.0%
GATGTTGGATGGGAAAGGCG+TGG - Chr3:90312656-90312675 MsG0380016985.01.T01:CDS 55.0%
! GTTGGATGGGAAAGGCGTGG+AGG - Chr3:90312659-90312678 MsG0380016985.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 90312621 90312947 90312621 ID=MsG0380016985.01;Name=MsG0380016985.01
Chr3 mRNA 90312621 90312947 90312621 ID=MsG0380016985.01.T01;Parent=MsG0380016985.01;Name=MsG0380016985.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|108
Chr3 exon 90312621 90312947 90312621 ID=MsG0380016985.01.T01:exon:5784;Parent=MsG0380016985.01.T01
Chr3 CDS 90312621 90312947 90312621 ID=MsG0380016985.01.T01:cds;Parent=MsG0380016985.01.T01
Gene Sequence

>MsG0380016985.01.T01

ATGGGGGAATGTGGGTGTGTTCCTTCTTTGATTAAGATGTTGGATGGGAAAGGCGTGGAGGAGAAAGAATCTGCTGCAATGGCGTTGTCGGTGTTGTTGCAGCACCCTTTCAATAGAAGGATCTTTAGGAAGGATGAGAGGGGAATAGTGAGTGCAGTTCACCTATTGAACCCTGCTTTGGTTAATTTGGATAAGAAATACCCTGTTTCGGTTCTGGTTTCACTTTTGCATTCGAAGACTTGTCGGAAACAAATGGTGGCTGCTGGGGCTTGTGTATATACGCAGAAGCTTGTTGAGTTGGATATTCCGGGTTCAAAGAAATGTTAG

Protein sequence

>MsG0380016985.01.T01

MGECGCVPSLIKMLDGKGVEEKESAAMALSVLLQHPFNRRIFRKDERGIVSAVHLLNPALVNLDKKYPVSVLVSLLHSKTCRKQMVAAGACVYTQKLVELDIPGSKKC*