AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480018519.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018519.01.T01 MTR_4g013350 95.455 198 8 1 1 198 1 197 1.68e-137 382
MsG0480018519.01.T01 MTR_4g013345 89.617 183 18 1 16 198 15 196 7.64e-122 343
MsG0480018519.01.T01 MTR_4g013330 83.152 184 29 1 17 198 16 199 6.46e-117 330
MsG0480018519.01.T01 MTR_4g013335 79.293 198 39 1 1 198 1 196 5.14e-112 318
MsG0480018519.01.T01 MTR_4g013320 78.261 184 38 1 17 198 16 199 1.88e-109 311
MsG0480018519.01.T01 MTR_4g013310 67.000 200 61 3 1 198 1 197 3.32e-92 268
MsG0480018519.01.T01 MTR_4g013325 75.622 201 44 3 1 198 1 199 5.88e-92 267
MsG0480018519.01.T01 MTR_7g070390 66.495 194 61 3 6 198 2 192 2.53e-82 243
MsG0480018519.01.T01 MTR_4g013385 60.317 189 69 3 10 198 8 190 4.79e-77 229
MsG0480018519.01.T01 MTR_0433s0040 60.317 189 69 3 10 198 8 190 4.79e-77 229
MsG0480018519.01.T01 MTR_4g013770 62.353 170 61 2 30 198 24 191 5.92e-70 211
MsG0480018519.01.T01 MTR_1g054525 56.633 196 75 3 3 198 7 192 8.40e-70 211
MsG0480018519.01.T01 MTR_4g013355 54.497 189 71 3 10 198 6 179 1.02e-64 197
MsG0480018519.01.T01 MTR_4g073950 55.921 152 66 1 48 198 41 192 7.51e-61 188
MsG0480018519.01.T01 MTR_7g093850 50.000 186 90 3 14 198 11 194 9.91e-59 183
MsG0480018519.01.T01 MTR_7g093820 50.000 186 90 3 14 198 11 194 1.48e-58 182
MsG0480018519.01.T01 MTR_7g093830 47.849 186 94 3 14 198 10 193 2.79e-57 179
MsG0480018519.01.T01 MTR_7g093790 48.795 166 83 2 34 198 27 191 2.89e-57 179
MsG0480018519.01.T01 MTR_7g093870 53.846 156 67 2 48 198 38 193 6.55e-55 174
MsG0480018519.01.T01 MTR_3g105640 48.447 161 81 2 40 198 28 188 4.60e-48 155
MsG0480018519.01.T01 MTR_1g056370 49.669 151 74 2 48 197 36 185 5.62e-48 155
MsG0480018519.01.T01 MTR_3g105630 42.487 193 100 3 9 198 5 189 9.11e-47 152
MsG0480018519.01.T01 MTR_4g078885 46.980 149 77 1 50 198 39 185 2.14e-44 146
MsG0480018519.01.T01 MTR_3g034030 47.651 149 76 1 50 198 39 185 4.73e-44 145
MsG0480018519.01.T01 MTR_5g096120 44.231 156 75 3 51 198 38 189 1.16e-40 137
MsG0480018519.01.T01 MTR_4g122110 36.620 142 90 0 57 198 39 180 9.90e-28 103
MsG0480018519.01.T01 MTR_4g122130 35.915 142 91 0 57 198 39 180 4.60e-25 96.7
MsG0480018519.01.T01 MTR_4g049550 31.293 147 101 0 47 193 27 173 4.48e-18 78.2
MsG0480018519.01.T01 MTR_4g049570 30.769 143 99 0 51 193 31 173 4.06e-17 75.9
MsG0480018519.01.T01 MTR_8g099115 33.088 136 82 3 50 177 34 168 1.45e-15 72.0
MsG0480018519.01.T01 MTR_8g099135 34.091 132 70 4 58 177 38 164 2.02e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480018519.01.T01 AT1G55210 60.317 189 70 2 10 198 4 187 7.55e-80 236
MsG0480018519.01.T01 AT1G55210 60.317 189 70 2 10 198 4 187 7.55e-80 236
MsG0480018519.01.T01 AT1G58170 56.915 188 70 2 13 198 7 185 2.07e-74 222
MsG0480018519.01.T01 AT5G49040 53.403 191 89 0 8 198 1 191 5.86e-74 221
MsG0480018519.01.T01 AT3G13650 51.832 191 87 3 8 198 1 186 7.79e-67 203
MsG0480018519.01.T01 AT5G49030 53.107 177 83 0 8 184 1 177 2.05e-58 197
MsG0480018519.01.T01 AT1G65870 52.318 151 72 0 47 197 39 189 9.92e-56 175
MsG0480018519.01.T01 AT2G21100 52.349 149 71 0 49 197 39 187 1.27e-54 172
MsG0480018519.01.T01 AT2G21100 52.349 149 71 0 49 197 39 187 1.27e-54 172
MsG0480018519.01.T01 AT3G13662 54.362 149 65 1 37 185 27 172 6.58e-53 167
MsG0480018519.01.T01 AT3G13660 63.559 118 43 0 81 198 8 125 7.57e-51 160
MsG0480018519.01.T01 AT1G22900 50.993 151 72 1 47 197 45 193 3.17e-50 161
MsG0480018519.01.T01 AT5G42500 48.344 151 74 2 48 197 38 185 3.91e-47 153
MsG0480018519.01.T01 AT5G42510 47.020 151 76 2 48 197 35 182 4.36e-46 150
MsG0480018519.01.T01 AT4G38700 44.872 156 80 3 49 198 35 190 1.54e-40 136
MsG0480018519.01.T01 AT3G58090 51.818 110 53 0 88 197 162 271 1.78e-37 131
MsG0480018519.01.T01 AT2G21110 38.235 170 95 2 33 198 23 186 2.11e-37 128
MsG0480018519.01.T01 AT4G11210 28.729 181 114 4 1 181 1 166 3.26e-16 73.6
MsG0480018519.01.T01 AT4G23690 36.364 132 77 3 46 173 34 162 4.97e-16 73.2
MsG0480018519.01.T01 AT4G11180 29.703 202 118 7 1 197 1 183 1.11e-11 61.2

Find 36 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTCTGGTCCTAAAGTTAA+AGG 0.191161 4:+6078349 None:intergenic
CATTGGATTGATTTCAAAAC+TGG 0.225984 4:-6078100 MsG0480018519.01.T01:CDS
ATTGACAACCCTTTAACTTT+AGG 0.265955 4:-6078358 MsG0480018519.01.T01:CDS
TTCATTGAAGGAAAGTATAA+TGG 0.275934 4:-6078238 MsG0480018519.01.T01:CDS
CCAATAGATCGCAAGTTGTT+AGG 0.329140 4:-6078520 MsG0480018519.01.T01:CDS
CCTAACAACTTGCGATCTAT+TGG 0.333660 4:+6078520 None:intergenic
GTAAACTCAACCACTGCTTT+TGG 0.339769 4:-6078394 MsG0480018519.01.T01:CDS
TAAGTCATTTCAGATTCTAT+TGG 0.351815 4:-6078477 MsG0480018519.01.T01:CDS
GAGGAATCCTGCTATTAATA+TGG 0.364770 4:-6078194 MsG0480018519.01.T01:CDS
ATGCCTGTTGTTGGTGGTAG+TGG 0.375988 4:-6078163 MsG0480018519.01.T01:CDS
GAACTGAGTTCTAAACTTGT+TGG 0.385159 4:-6078331 MsG0480018519.01.T01:CDS
GGGAGTACTCTTACTATTGT+AGG 0.399599 4:-6078217 MsG0480018519.01.T01:CDS
GTTCTGGTCCTAAAGTTAAA+GGG 0.426776 4:+6078350 None:intergenic
ACAAGTTTAGAACTCAGTTC+TGG 0.427865 4:+6078334 None:intergenic
GGAACAATTGCAACCGAAGT+TGG 0.428664 4:+6078430 None:intergenic
TCATTGAAGGAAAGTATAAT+GGG 0.435892 4:-6078237 MsG0480018519.01.T01:CDS
ACAAAGTCATGATGAAATGT+TGG 0.438715 4:+6078623 None:intergenic
ATCCTGCTATTAATATGGTT+AGG 0.453886 4:-6078189 MsG0480018519.01.T01:CDS
ATGCTCAAGCTAATACTCAT+TGG 0.456460 4:-6078117 MsG0480018519.01.T01:CDS
CAAAGTCATGATGAAATGTT+GGG 0.470092 4:+6078624 None:intergenic
AAACTTGTTGGAAGAGCTCA+AGG 0.479877 4:-6078319 MsG0480018519.01.T01:CDS
ATTCTATGCATCTGCATCAC+AGG 0.484694 4:-6078296 MsG0480018519.01.T01:CDS
TCCTGCTATTAATATGGTTA+GGG 0.491633 4:-6078188 MsG0480018519.01.T01:CDS
GTTAGGGAGATGCCTGTTGT+TGG 0.492441 4:-6078172 MsG0480018519.01.T01:CDS
AGTCCACTACCACCAACAAC+AGG 0.512977 4:+6078160 None:intergenic
AGGGAGATGCCTGTTGTTGG+TGG 0.521357 4:-6078169 MsG0480018519.01.T01:CDS
GGAGTACTCTTACTATTGTA+GGG 0.530187 4:-6078216 MsG0480018519.01.T01:CDS
TCCCTAACCATATTAATAGC+AGG 0.536411 4:+6078187 None:intergenic
TATTGGCATGACATCTTGAG+TGG 0.547551 4:-6078460 MsG0480018519.01.T01:CDS
TCTGCATCACAGGAAGAACT+AGG 0.571860 4:-6078286 MsG0480018519.01.T01:CDS
GTCATGATGAAATGTTGGGA+AGG 0.583236 4:+6078628 None:intergenic
ATTGGATTGATTTCAAAACT+GGG 0.602248 4:-6078099 MsG0480018519.01.T01:CDS
TTGGATTGATTTCAAAACTG+GGG 0.621102 4:-6078098 MsG0480018519.01.T01:CDS
GTGTGTTGCAAGATATGAGA+AGG 0.624292 4:+6078593 None:intergenic
GTTGACTAAACCAAAAGCAG+TGG 0.670239 4:+6078384 None:intergenic
GTACTCTTACTATTGTAGGG+AGG 0.687219 4:-6078213 MsG0480018519.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAGTCATTTCAGATTCTAT+TGG - Chr4:6078211-6078230 MsG0480018519.01.T01:CDS 25.0%
!! ATGAATTTTGCTTTCATTGA+AGG - Chr4:6078438-6078457 MsG0480018519.01.T01:CDS 25.0%
!! ATTGGATTGATTTCAAAACT+GGG - Chr4:6078589-6078608 MsG0480018519.01.T01:CDS 25.0%
!! TCATTGAAGGAAAGTATAAT+GGG - Chr4:6078451-6078470 MsG0480018519.01.T01:CDS 25.0%
!! TTCATTGAAGGAAAGTATAA+TGG - Chr4:6078450-6078469 MsG0480018519.01.T01:CDS 25.0%
ACAAAGTCATGATGAAATGT+TGG + Chr4:6078068-6078087 None:intergenic 30.0%
ATCCTGCTATTAATATGGTT+AGG - Chr4:6078499-6078518 MsG0480018519.01.T01:CDS 30.0%
CAAAGTCATGATGAAATGTT+GGG + Chr4:6078067-6078086 None:intergenic 30.0%
TCCTGCTATTAATATGGTTA+GGG - Chr4:6078500-6078519 MsG0480018519.01.T01:CDS 30.0%
! ATTGACAACCCTTTAACTTT+AGG - Chr4:6078330-6078349 MsG0480018519.01.T01:CDS 30.0%
! CATTGGATTGATTTCAAAAC+TGG - Chr4:6078588-6078607 MsG0480018519.01.T01:CDS 30.0%
! TTGGATTGATTTCAAAACTG+GGG - Chr4:6078590-6078609 MsG0480018519.01.T01:CDS 30.0%
!! GTTGAGTTTACTTTTGATGA+TGG + Chr4:6078285-6078304 None:intergenic 30.0%
ATGCTCAAGCTAATACTCAT+TGG - Chr4:6078571-6078590 MsG0480018519.01.T01:CDS 35.0%
GAGGAATCCTGCTATTAATA+TGG - Chr4:6078494-6078513 MsG0480018519.01.T01:CDS 35.0%
GAGTGGAAAAAATCCAACTT+CGG - Chr4:6078245-6078264 MsG0480018519.01.T01:CDS 35.0%
GTTCTGGTCCTAAAGTTAAA+GGG + Chr4:6078341-6078360 None:intergenic 35.0%
TCCCTAACCATATTAATAGC+AGG + Chr4:6078504-6078523 None:intergenic 35.0%
! ACAAGTTTAGAACTCAGTTC+TGG + Chr4:6078357-6078376 None:intergenic 35.0%
! AGTTCTGGTCCTAAAGTTAA+AGG + Chr4:6078342-6078361 None:intergenic 35.0%
! GGAGTACTCTTACTATTGTA+GGG - Chr4:6078472-6078491 MsG0480018519.01.T01:CDS 35.0%
!! GAACTGAGTTCTAAACTTGT+TGG - Chr4:6078357-6078376 MsG0480018519.01.T01:CDS 35.0%
!!! GAGTTTACTTTTGATGATGG+TGG + Chr4:6078282-6078301 None:intergenic 35.0%
ATTCTATGCATCTGCATCAC+AGG - Chr4:6078392-6078411 MsG0480018519.01.T01:CDS 40.0%
CCAATAGATCGCAAGTTGTT+AGG - Chr4:6078168-6078187 MsG0480018519.01.T01:CDS 40.0%
CCTAACAACTTGCGATCTAT+TGG + Chr4:6078171-6078190 None:intergenic 40.0%
GTACTCTTACTATTGTAGGG+AGG - Chr4:6078475-6078494 MsG0480018519.01.T01:CDS 40.0%
GTCATGATGAAATGTTGGGA+AGG + Chr4:6078063-6078082 None:intergenic 40.0%
GTGTGTTGCAAGATATGAGA+AGG + Chr4:6078098-6078117 None:intergenic 40.0%
GTTGACTAAACCAAAAGCAG+TGG + Chr4:6078307-6078326 None:intergenic 40.0%
TATTGGCATGACATCTTGAG+TGG - Chr4:6078228-6078247 MsG0480018519.01.T01:CDS 40.0%
! GTAAACTCAACCACTGCTTT+TGG - Chr4:6078294-6078313 MsG0480018519.01.T01:CDS 40.0%
!! AAACTTGTTGGAAGAGCTCA+AGG - Chr4:6078369-6078388 MsG0480018519.01.T01:CDS 40.0%
!! GGGAGTACTCTTACTATTGT+AGG - Chr4:6078471-6078490 MsG0480018519.01.T01:CDS 40.0%
!!! GGACTTTTCAGATTTGCTAG+AGG - Chr4:6078546-6078565 MsG0480018519.01.T01:CDS 40.0%
GGAACAATTGCAACCGAAGT+TGG + Chr4:6078261-6078280 None:intergenic 45.0%
TCTGCATCACAGGAAGAACT+AGG - Chr4:6078402-6078421 MsG0480018519.01.T01:CDS 45.0%
AGTCCACTACCACCAACAAC+AGG + Chr4:6078531-6078550 None:intergenic 50.0%
GTTAGGGAGATGCCTGTTGT+TGG - Chr4:6078516-6078535 MsG0480018519.01.T01:CDS 50.0%
!! ATGCCTGTTGTTGGTGGTAG+TGG - Chr4:6078525-6078544 MsG0480018519.01.T01:CDS 50.0%
AGGGAGATGCCTGTTGTTGG+TGG - Chr4:6078519-6078538 MsG0480018519.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 6078057 6078653 6078057 ID=MsG0480018519.01;Name=MsG0480018519.01
Chr4 mRNA 6078057 6078653 6078057 ID=MsG0480018519.01.T01;Parent=MsG0480018519.01;Name=MsG0480018519.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|198
Chr4 exon 6078057 6078653 6078057 ID=MsG0480018519.01.T01:exon:2154;Parent=MsG0480018519.01.T01
Chr4 CDS 6078057 6078653 6078057 ID=MsG0480018519.01.T01:cds;Parent=MsG0480018519.01.T01
Gene Sequence

>MsG0480018519.01.T01

ATGCCTTCCCAACATTTCATCATGACTTTGTTCTTCTTCCTTCTCATATCTTGCAACACACTCACTGCTTCAACTTCGTCAGAAAAGCAAGACACTTTCGATTTTGTTCGTCCAATAGATCGCAAGTTGTTAGGCTTAAACAAGAAAGAAAAGCTAAGTCATTTCAGATTCTATTGGCATGACATCTTGAGTGGAAAAAATCCAACTTCGGTTGCAATTGTTCCACCATCATCAAAAGTAAACTCAACCACTGCTTTTGGTTTAGTCAACATGATTGACAACCCTTTAACTTTAGGACCAGAACTGAGTTCTAAACTTGTTGGAAGAGCTCAAGGATTCTATGCATCTGCATCACAGGAAGAACTAGGTTTTCTTATGACAATGAATTTTGCTTTCATTGAAGGAAAGTATAATGGGAGTACTCTTACTATTGTAGGGAGGAATCCTGCTATTAATATGGTTAGGGAGATGCCTGTTGTTGGTGGTAGTGGACTTTTCAGATTTGCTAGAGGATATGCTCAAGCTAATACTCATTGGATTGATTTCAAAACTGGGGATGCTTGTGTTGAGTACAATGTTCATGTTTTTCATTATTGA

Protein sequence

>MsG0480018519.01.T01

MPSQHFIMTLFFFLLISCNTLTASTSSEKQDTFDFVRPIDRKLLGLNKKEKLSHFRFYWHDILSGKNPTSVAIVPPSSKVNSTTAFGLVNMIDNPLTLGPELSSKLVGRAQGFYASASQEELGFLMTMNFAFIEGKYNGSTLTIVGRNPAINMVREMPVVGGSGLFRFARGYAQANTHWIDFKTGDACVEYNVHVFHY*