Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000794.01.T01 | XP_003588536.2 | 96.324 | 136 | 5 | 0 | 6 | 141 | 150 | 285 | 1.43E-84 | 259 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000794.01.T01 | Q84MB5 | 80.147 | 136 | 27 | 0 | 6 | 141 | 139 | 274 | 2.43E-63 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000794.01.T01 | G7I4B9 | 96.324 | 136 | 5 | 0 | 6 | 141 | 150 | 285 | 6.81e-85 | 259 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000794.01 | MsG0180005303.01 | -0.810499 | 1.118253e-50 | 6.751511e-48 |
| MsG0180000794.01 | MsG0180006096.01 | 0.800400 | 1.466970e-48 | 6.824141e-46 |
| MsG0180000794.01 | MsG0280007547.01 | 0.803284 | 3.751728e-49 | 1.877814e-46 |
| MsG0180000794.01 | MsG0280007903.01 | 0.814257 | 1.689653e-51 | 1.128183e-48 |
| MsG0180000794.01 | MsG0280011376.01 | 0.804120 | 2.516059e-49 | 1.286748e-46 |
| MsG0180000794.01 | MsG0380015166.01 | 0.813501 | 2.478910e-51 | 1.621681e-48 |
| MsG0180000794.01 | MsG0380015533.01 | 0.800495 | 1.403466e-48 | 6.544312e-46 |
| MsG0180000794.01 | MsG0380016740.01 | 0.835511 | 1.608064e-56 | 1.970693e-53 |
| MsG0180000794.01 | MsG0380017085.01 | 0.812880 | 3.393706e-51 | 2.182993e-48 |
| MsG0180000794.01 | MsG0380017126.01 | 0.804490 | 2.106642e-49 | 1.087615e-46 |
| MsG0180000794.01 | MsG0480018402.01 | 0.802267 | 6.084175e-49 | 2.967387e-46 |
| MsG0180000794.01 | MsG0480018911.01 | 0.829898 | 3.978364e-55 | 4.127033e-52 |
| MsG0180000794.01 | MsG0480020024.01 | 0.803231 | 3.848396e-49 | 1.923586e-46 |
| MsG0180000794.01 | MsG0480020788.01 | 0.804110 | 2.527959e-49 | 1.292495e-46 |
| MsG0180000794.01 | MsG0480021768.01 | 0.813864 | 2.062399e-51 | 1.362479e-48 |
| MsG0180000794.01 | MsG0580024161.01 | 0.824110 | 9.637432e-54 | 8.456729e-51 |
| MsG0180000794.01 | MsG0580029919.01 | 0.820919 | 5.317924e-53 | 4.264538e-50 |
| MsG0180000794.01 | MsG0780036880.01 | 0.801140 | 1.036413e-48 | 4.912632e-46 |
| MsG0180000794.01 | MsG0780036978.01 | 0.805157 | 1.528926e-49 | 8.030636e-47 |
| MsG0180000794.01 | MsG0780041435.01 | 0.806979 | 6.321690e-50 | 3.479336e-47 |
| MsG0180000794.01 | MsG0880047703.01 | 0.801633 | 8.213135e-49 | 3.941557e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000794.01.T01 | MTR_1g008300 | 96.324 | 136 | 5 | 0 | 6 | 141 | 150 | 285 | 1.73e-88 | 259 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000794.01.T01 | AT5G25640 | 59.712 | 139 | 47 | 1 | 6 | 135 | 146 | 284 | 2.20e-38 | 131 |
Find 36 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCATATTGCACACCTTTC+TGG | 0.256823 | 1:-11262216 | MsG0180000794.01.T01:CDS |
| GATCCTTGAGGTGCTTATAT+TGG | 0.267418 | 1:-11262967 | MsG0180000794.01.T01:CDS |
| TTGGAGCAAACCTTGTTTCC+TGG | 0.280672 | 1:-11263182 | MsG0180000794.01.T01:CDS |
| TGGCTTTCTTACATTCTTAC+CGG | 0.307877 | 1:-11263207 | MsG0180000794.01.T01:CDS |
| CCAACTGAAACAGTGTTTCT+TGG | 0.313482 | 1:+11263150 | None:intergenic |
| ACTGTTTCAGTTGGAGCTTC+TGG | 0.317045 | 1:-11263141 | MsG0180000794.01.T01:CDS |
| CACCTTTCTGGTGCTCTTGT+TGG | 0.320132 | 1:-11262204 | MsG0180000794.01.T01:CDS |
| ATCCTTGAGGTGCTTATATT+GGG | 0.342592 | 1:-11262966 | MsG0180000794.01.T01:CDS |
| TGCTCTTGTTGGTGTGCTCT+TGG | 0.361990 | 1:-11262193 | MsG0180000794.01.T01:CDS |
| CACCAGAAAGGTGTGCAATA+TGG | 0.382272 | 1:+11262214 | None:intergenic |
| TATTTCGTTGCTTTCACAAC+AGG | 0.404039 | 1:-11263267 | MsG0180000794.01.T01:CDS |
| TTGTTGGTGTGCTCTTGGTT+TGG | 0.405808 | 1:-11262188 | MsG0180000794.01.T01:CDS |
| TGATTTAGATGTCATGGGAT+TGG | 0.421546 | 1:-11262995 | MsG0180000794.01.T01:intron |
| TAGCCTCCACGAAAGGTTCC+TGG | 0.429863 | 1:+11262255 | None:intergenic |
| CAATTTCATGTACCTTTACA+AGG | 0.431794 | 1:+11263088 | None:intergenic |
| TCTTACATTCTTACCGGTGT+TGG | 0.432285 | 1:-11263201 | MsG0180000794.01.T01:CDS |
| AGGAAAGCTTGTTGAAGAAG+AGG | 0.467402 | 1:-11263247 | MsG0180000794.01.T01:CDS |
| TCTTTACCAGGAACCTTTCG+TGG | 0.471320 | 1:-11262261 | MsG0180000794.01.T01:CDS |
| TACTATCAGCGTCCTTGTAA+AGG | 0.478499 | 1:-11263100 | MsG0180000794.01.T01:intron |
| AACAAGGTTTGCTCCAACAC+CGG | 0.484733 | 1:+11263188 | None:intergenic |
| CCAAGAAACACTGTTTCAGT+TGG | 0.488646 | 1:-11263150 | MsG0180000794.01.T01:CDS |
| GGGTCAGTTTGTTGTAGAGA+AGG | 0.498677 | 1:-11262946 | MsG0180000794.01.T01:intron |
| GAACTTGATTTAGATGTCAT+GGG | 0.502041 | 1:-11263000 | MsG0180000794.01.T01:intron |
| TGAACTTGATTTAGATGTCA+TGG | 0.507252 | 1:-11263001 | MsG0180000794.01.T01:intron |
| TTTAGATGTCATGGGATTGG+AGG | 0.517050 | 1:-11262992 | MsG0180000794.01.T01:intron |
| CAAGCTTCAACATCTTTACC+AGG | 0.545205 | 1:-11262273 | MsG0180000794.01.T01:CDS |
| GTTTCTTGGTAAAATCAGCC+AGG | 0.562273 | 1:+11263164 | None:intergenic |
| GTAAAATCAGCCAGGAAACA+AGG | 0.565010 | 1:+11263172 | None:intergenic |
| CACCAACAAGAGCACCAGAA+AGG | 0.589348 | 1:+11262202 | None:intergenic |
| GGAAAGCTTGTTGAAGAAGA+GGG | 0.589617 | 1:-11263246 | MsG0180000794.01.T01:CDS |
| AAGCTTGTTGAAGAAGAGGG+AGG | 0.592709 | 1:-11263243 | MsG0180000794.01.T01:CDS |
| CAATGCGTAGCCTCCACGAA+AGG | 0.605361 | 1:+11262248 | None:intergenic |
| TATCGGTCTGAAGAATCAGA+AGG | 0.633251 | 1:+11262150 | None:intergenic |
| TTACCAGGAACCTTTCGTGG+AGG | 0.643485 | 1:-11262258 | MsG0180000794.01.T01:CDS |
| GACCCAATATAAGCACCTCA+AGG | 0.644183 | 1:+11262964 | None:intergenic |
| GGATTGGAGGAAGATCCTTG+AGG | 0.747907 | 1:-11262979 | MsG0180000794.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGATACATATTGAAATTC+TGG | - | Chr1:11262631-11262650 | MsG0180000794.01.T01:intron | 20.0% |
| !! | ATAACAAACTACAAAATAGT+TGG | - | Chr1:11262779-11262798 | MsG0180000794.01.T01:intron | 20.0% |
| !! | GAATTTCAATATGTATCTTT+AGG | + | Chr1:11262631-11262650 | None:intergenic | 20.0% |
| !! | GGAAAAAAGAAAGAAAAAAA+AGG | + | Chr1:11262521-11262540 | None:intergenic | 20.0% |
| !! | AATCATTTAGAGTTGTTTAC+AGG | + | Chr1:11262551-11262570 | None:intergenic | 25.0% |
| !! | AGATAAAAAAGCTCATGTTT+TGG | - | Chr1:11263034-11263053 | MsG0180000794.01.T01:intron | 25.0% |
| !! | ATCATTTAGAGTTGTTTACA+GGG | + | Chr1:11262550-11262569 | None:intergenic | 25.0% |
| AATATATTGCAAGCCATTTG+CGG | + | Chr1:11262963-11262982 | None:intergenic | 30.0% | |
| ACATATTTGACACATCTGAA+GGG | + | Chr1:11262876-11262895 | None:intergenic | 30.0% | |
| CAATTTCATGTACCTTTACA+AGG | + | Chr1:11262343-11262362 | None:intergenic | 30.0% | |
| GAACTTGATTTAGATGTCAT+GGG | - | Chr1:11262428-11262447 | MsG0180000794.01.T01:intron | 30.0% | |
| TACATATTTGACACATCTGA+AGG | + | Chr1:11262877-11262896 | None:intergenic | 30.0% | |
| TGAACTTGATTTAGATGTCA+TGG | - | Chr1:11262427-11262446 | MsG0180000794.01.T01:intron | 30.0% | |
| ! | AACCAAAAAGTGTAAGAACT+TGG | + | Chr1:11262408-11262427 | None:intergenic | 30.0% |
| ! | CTGTTATTTGTGAGTGTAAT+AGG | - | Chr1:11263118-11263137 | MsG0180000794.01.T01:CDS | 30.0% |
| !!! | ATGTTTTGGTCTTGTTATTC+GGG | - | Chr1:11263048-11263067 | MsG0180000794.01.T01:intron | 30.0% |
| !!! | CATGTTTTGGTCTTGTTATT+CGG | - | Chr1:11263047-11263066 | MsG0180000794.01.T01:intron | 30.0% |
| AAGAACTTGGAGAAACAAAC+TGG | + | Chr1:11262395-11262414 | None:intergenic | 35.0% | |
| AGCACACTCATGAAATTTAC+AGG | + | Chr1:11262658-11262677 | None:intergenic | 35.0% | |
| GGGTTTATACTTGAACAACT+AGG | - | Chr1:11263068-11263087 | MsG0180000794.01.T01:intron | 35.0% | |
| TGATTTAGATGTCATGGGAT+TGG | - | Chr1:11262433-11262452 | MsG0180000794.01.T01:intron | 35.0% | |
| TGGCTTTCTTACATTCTTAC+CGG | - | Chr1:11262221-11262240 | MsG0180000794.01.T01:CDS | 35.0% | |
| TTAGGAGATGCAAATCTGAA+AGG | + | Chr1:11262613-11262632 | None:intergenic | 35.0% | |
| TTTGTGAGTGTAATAGGTCA+TGG | - | Chr1:11263124-11263143 | MsG0180000794.01.T01:CDS | 35.0% | |
| ! | ATCCTTGAGGTGCTTATATT+GGG | - | Chr1:11262462-11262481 | MsG0180000794.01.T01:intron | 35.0% |
| ! | CTCCAAGTTCTTACACTTTT+TGG | - | Chr1:11262403-11262422 | MsG0180000794.01.T01:intron | 35.0% |
| ! | TATTTCGTTGCTTTCACAAC+AGG | - | Chr1:11262161-11262180 | MsG0180000794.01.T01:CDS | 35.0% |
| AACCATATTGCACACCTTTC+TGG | - | Chr1:11263212-11263231 | MsG0180000794.01.T01:CDS | 40.0% | |
| CAAGCTTCAACATCTTTACC+AGG | - | Chr1:11263155-11263174 | MsG0180000794.01.T01:CDS | 40.0% | |
| CCAACTGAAACAGTGTTTCT+TGG | + | Chr1:11262281-11262300 | None:intergenic | 40.0% | |
| CCAAGAAACACTGTTTCAGT+TGG | - | Chr1:11262278-11262297 | MsG0180000794.01.T01:CDS | 40.0% | |
| GAGTTGTTTACAGGGCAAAA+CGG | + | Chr1:11262542-11262561 | None:intergenic | 40.0% | |
| GTAAAATCAGCCAGGAAACA+AGG | + | Chr1:11262259-11262278 | None:intergenic | 40.0% | |
| GTTTCTTGGTAAAATCAGCC+AGG | + | Chr1:11262267-11262286 | None:intergenic | 40.0% | |
| TACTATCAGCGTCCTTGTAA+AGG | - | Chr1:11262328-11262347 | MsG0180000794.01.T01:intron | 40.0% | |
| TATCGGTCTGAAGAATCAGA+AGG | + | Chr1:11263281-11263300 | None:intergenic | 40.0% | |
| TCTTACATTCTTACCGGTGT+TGG | - | Chr1:11262227-11262246 | MsG0180000794.01.T01:CDS | 40.0% | |
| TTTAGATGTCATGGGATTGG+AGG | - | Chr1:11262436-11262455 | MsG0180000794.01.T01:intron | 40.0% | |
| ! | CATCTGCGTGTATTTTATCG+CGG | + | Chr1:11262758-11262777 | None:intergenic | 40.0% |
| ! | GATCCTTGAGGTGCTTATAT+TGG | - | Chr1:11262461-11262480 | MsG0180000794.01.T01:intron | 40.0% |
| !! | AGGAAAGCTTGTTGAAGAAG+AGG | - | Chr1:11262181-11262200 | MsG0180000794.01.T01:CDS | 40.0% |
| !! | GGAAAGCTTGTTGAAGAAGA+GGG | - | Chr1:11262182-11262201 | MsG0180000794.01.T01:CDS | 40.0% |
| ACTGTTTCAGTTGGAGCTTC+TGG | - | Chr1:11262287-11262306 | MsG0180000794.01.T01:CDS | 45.0% | |
| GACCCAATATAAGCACCTCA+AGG | + | Chr1:11262467-11262486 | None:intergenic | 45.0% | |
| TCTTTACCAGGAACCTTTCG+TGG | - | Chr1:11263167-11263186 | MsG0180000794.01.T01:CDS | 45.0% | |
| TTGTTGGTGTGCTCTTGGTT+TGG | - | Chr1:11263240-11263259 | MsG0180000794.01.T01:CDS | 45.0% | |
| ! | AACAAGGTTTGCTCCAACAC+CGG | + | Chr1:11262243-11262262 | None:intergenic | 45.0% |
| ! | AGGGAGGAAGTTTTGCTTTG+TGG | - | Chr1:11262201-11262220 | MsG0180000794.01.T01:CDS | 45.0% |
| ! | CACCAGAAAGGTGTGCAATA+TGG | + | Chr1:11263217-11263236 | None:intergenic | 45.0% |
| ! | TTGGAGCAAACCTTGTTTCC+TGG | - | Chr1:11262246-11262265 | MsG0180000794.01.T01:CDS | 45.0% |
| !! | AAGCTTGTTGAAGAAGAGGG+AGG | - | Chr1:11262185-11262204 | MsG0180000794.01.T01:CDS | 45.0% |
| !! | ATCTGAAGGGAGCACTTTAG+AGG | + | Chr1:11262863-11262882 | None:intergenic | 45.0% |
| !! | GGGTCAGTTTGTTGTAGAGA+AGG | - | Chr1:11262482-11262501 | MsG0180000794.01.T01:intron | 45.0% |
| !!! | GAGCTTCTGGTGCTGTTTTT+GGG | - | Chr1:11262300-11262319 | MsG0180000794.01.T01:intron | 45.0% |
| GGATTGGAGGAAGATCCTTG+AGG | - | Chr1:11262449-11262468 | MsG0180000794.01.T01:intron | 50.0% | |
| TGCTCTTGTTGGTGTGCTCT+TGG | - | Chr1:11263235-11263254 | MsG0180000794.01.T01:CDS | 50.0% | |
| TGCTTGACTCATGCCGCAAA+TGG | - | Chr1:11262947-11262966 | MsG0180000794.01.T01:CDS | 50.0% | |
| TTACCAGGAACCTTTCGTGG+AGG | - | Chr1:11263170-11263189 | MsG0180000794.01.T01:CDS | 50.0% | |
| ! | CACCTTTCTGGTGCTCTTGT+TGG | - | Chr1:11263224-11263243 | MsG0180000794.01.T01:CDS | 50.0% |
| !! | CACCAACAAGAGCACCAGAA+AGG | + | Chr1:11263229-11263248 | None:intergenic | 50.0% |
| !!! | GGAGCTTCTGGTGCTGTTTT+TGG | - | Chr1:11262299-11262318 | MsG0180000794.01.T01:intron | 50.0% |
| CAATGCGTAGCCTCCACGAA+AGG | + | Chr1:11263183-11263202 | None:intergenic | 55.0% | |
| TAGCCTCCACGAAAGGTTCC+TGG | + | Chr1:11263176-11263195 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 11262149 | 11263301 | 11262149 | ID=MsG0180000794.01;Name=MsG0180000794.01 |
| Chr1 | mRNA | 11262149 | 11263301 | 11262149 | ID=MsG0180000794.01.T01;Parent=MsG0180000794.01;Name=MsG0180000794.01.T01;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|3|0|141 |
| Chr1 | exon | 11263101 | 11263301 | 11263101 | ID=MsG0180000794.01.T01:exon:865;Parent=MsG0180000794.01.T01 |
| Chr1 | exon | 11262947 | 11263009 | 11262947 | ID=MsG0180000794.01.T01:exon:866;Parent=MsG0180000794.01.T01 |
| Chr1 | exon | 11262149 | 11262310 | 11262149 | ID=MsG0180000794.01.T01:exon:867;Parent=MsG0180000794.01.T01 |
| Chr1 | CDS | 11263101 | 11263301 | 11263101 | ID=MsG0180000794.01.T01:cds;Parent=MsG0180000794.01.T01 |
| Chr1 | CDS | 11262947 | 11263009 | 11262947 | ID=MsG0180000794.01.T01:cds;Parent=MsG0180000794.01.T01 |
| Chr1 | CDS | 11262149 | 11262310 | 11262149 | ID=MsG0180000794.01.T01:cds;Parent=MsG0180000794.01.T01 |
| Gene Sequence |
| Protein sequence |
