AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015166.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015166.01.T01 MTR_3g064240 100.000 82 0 0 1 82 1 82 1.63e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015166.01.T01 AT4G21192 75.000 80 20 0 1 80 1 80 6.26e-42 132
MsG0380015166.01.T01 AT4G21192 75.000 80 20 0 1 80 1 80 6.26e-42 132

Find 9 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTATTGCAAAATTCTT+TGG 0.235808 3:+66079659 MsG0380015166.01.T01:CDS
TACAGAATTGAAAATAAAAT+TGG 0.247760 3:+66079688 MsG0380015166.01.T01:CDS
GGATGTTTGTGCAATGTTAA+AGG 0.281917 3:-66078912 None:intergenic
ACAGAAAGCTGTGAAGAGAA+AGG 0.562696 3:+66079844 MsG0380015166.01.T01:intron
TGAAAAGAGCAAAGAACTCA+AGG 0.575643 3:+66079874 MsG0380015166.01.T01:CDS
AAACTCACTTCAGCGCACAT+AGG 0.624864 3:-66078933 None:intergenic
GGAGTTTCAAAAGTGTCATG+TGG 0.640065 3:+66079634 MsG0380015166.01.T01:CDS
GTGCTTAATGCAGATTATCG+AGG 0.649835 3:+66079613 MsG0380015166.01.T01:intron
TGTTTGTGCAATGTTAAAGG+AGG 0.660285 3:-66078909 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAACTTTAACATAAAAAATA+AGG - Chr3:66079765-66079784 None:intergenic 15.0%
!! TACAGAATTGAAAATAAAAT+TGG + Chr3:66079688-66079707 MsG0380015166.01.T01:CDS 15.0%
!!! AATTGATTCTAAATCCATTA+TGG - Chr3:66079493-66079512 None:intergenic 20.0%
!!! GATAATTTATCTTTTTCCTT+TGG + Chr3:66079539-66079558 MsG0380015166.01.T01:intron 20.0%
! CATTTCATTGTTTCAATATC+AGG - Chr3:66079136-66079155 None:intergenic 25.0%
! GAAATTAGTAGTTGAAAGTT+TGG + Chr3:66079098-66079117 MsG0380015166.01.T01:intron 25.0%
! TCAAATAAGTCAACTCAAAT+AGG - Chr3:66079168-66079187 None:intergenic 25.0%
!! GTACAAGTTATAAGCTATTT+TGG - Chr3:66079293-66079312 None:intergenic 25.0%
ATATTTCCATGTCCATGTTA+TGG + Chr3:66079068-66079087 MsG0380015166.01.T01:intron 30.0%
ATTTCCATTATCCCATAACA+TGG - Chr3:66079083-66079102 None:intergenic 30.0%
CATCCTATTGCAAAATTCTT+TGG + Chr3:66079659-66079678 MsG0380015166.01.T01:CDS 30.0%
GAAACAATGAAATGTGACTT+GGG + Chr3:66079142-66079161 MsG0380015166.01.T01:intron 30.0%
TATTTCCATGTCCATGTTAT+GGG + Chr3:66079069-66079088 MsG0380015166.01.T01:intron 30.0%
TGAAACAATGAAATGTGACT+TGG + Chr3:66079141-66079160 MsG0380015166.01.T01:intron 30.0%
TTAAAGTATGCTATCGCATA+AGG - Chr3:66079585-66079604 None:intergenic 30.0%
! AGGAACAATTACGAGTTTTA+AGG + Chr3:66079894-66079913 MsG0380015166.01.T01:CDS 30.0%
! TTCTTTGCTCTTTTCAAAGT+TGG - Chr3:66079870-66079889 None:intergenic 30.0%
ATACCACTTGTGAGACTATT+TGG + Chr3:66079202-66079221 MsG0380015166.01.T01:intron 35.0%
ATGTCCATGTTATGGGATAA+TGG + Chr3:66079076-66079095 MsG0380015166.01.T01:intron 35.0%
ATTATCCCATAACATGGACA+TGG - Chr3:66079077-66079096 None:intergenic 35.0%
CAATACATCTTAGTGGCTTA+AGG - Chr3:66079420-66079439 None:intergenic 35.0%
GCGATAGCATACTTTAATTC+AGG + Chr3:66079588-66079607 MsG0380015166.01.T01:intron 35.0%
GGATGTTTGTGCAATGTTAA+AGG - Chr3:66078915-66078934 None:intergenic 35.0%
TACCACTTGTGAGACTATTT+GGG + Chr3:66079203-66079222 MsG0380015166.01.T01:intron 35.0%
TGAAAAGAGCAAAGAACTCA+AGG + Chr3:66079874-66079893 MsG0380015166.01.T01:CDS 35.0%
TGTTTGTGCAATGTTAAAGG+AGG - Chr3:66078912-66078931 None:intergenic 35.0%
! ATTATGGAGTGCCAAAGTAA+GGG - Chr3:66079477-66079496 None:intergenic 35.0%
! TCGCCAAAGAATTTTGCAAT+AGG - Chr3:66079665-66079684 None:intergenic 35.0%
!! AGTTTGATCTGCTTTTCTTG+TGG + Chr3:66079795-66079814 MsG0380015166.01.T01:intron 35.0%
AACAAGCTGTTTACTGGAAC+AGG + Chr3:66079393-66079412 MsG0380015166.01.T01:intron 40.0%
ACAAGCTGTTTACTGGAACA+GGG + Chr3:66079394-66079413 MsG0380015166.01.T01:intron 40.0%
ACAGAAAGCTGTGAAGAGAA+AGG + Chr3:66079844-66079863 MsG0380015166.01.T01:intron 40.0%
ATGACAACTTTCAGCACCAA+AGG - Chr3:66079558-66079577 None:intergenic 40.0%
CAGTTGGAATTCCCTTACTT+TGG + Chr3:66079463-66079482 MsG0380015166.01.T01:intron 40.0%
CCACTAAGATGTATTGCCAA+GGG + Chr3:66079424-66079443 MsG0380015166.01.T01:intron 40.0%
CTTACTTTGGCACTCCATAA+TGG + Chr3:66079476-66079495 MsG0380015166.01.T01:intron 40.0%
GTGCTTAATGCAGATTATCG+AGG + Chr3:66079613-66079632 MsG0380015166.01.T01:intron 40.0%
TAAGGGAATTCCAACTGACA+GGG - Chr3:66079460-66079479 None:intergenic 40.0%
TGTTTGGCAGCTTATAGCAT+AGG - Chr3:66079377-66079396 None:intergenic 40.0%
TTCTCTTCACAGCTTTCTGT+TGG - Chr3:66079844-66079863 None:intergenic 40.0%
! CATTATGGAGTGCCAAAGTA+AGG - Chr3:66079478-66079497 None:intergenic 40.0%
! GTTCCAGTAAACAGCTTGTT+TGG - Chr3:66079393-66079412 None:intergenic 40.0%
!! GGAGTTTCAAAAGTGTCATG+TGG + Chr3:66079634-66079653 MsG0380015166.01.T01:CDS 40.0%
AAACTCACTTCAGCGCACAT+AGG - Chr3:66078936-66078955 None:intergenic 45.0%
CCCTTGGCAATACATCTTAG+TGG - Chr3:66079427-66079446 None:intergenic 45.0%
CTCCCAAATAGTCTCACAAG+TGG - Chr3:66079208-66079227 None:intergenic 45.0%
CTGCCAAACAAGCTGTTTAC+TGG + Chr3:66079387-66079406 MsG0380015166.01.T01:intron 45.0%
GCCACTAAGATGTATTGCCA+AGG + Chr3:66079423-66079442 MsG0380015166.01.T01:intron 45.0%
GTAAGGGAATTCCAACTGAC+AGG - Chr3:66079461-66079480 None:intergenic 45.0%
TTATCTGTTGCCCTGTCAGT+TGG + Chr3:66079447-66079466 MsG0380015166.01.T01:intron 45.0%
ACAGGGCAACAGATAACCCT+TGG - Chr3:66079443-66079462 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr3 gene 66078903 66079946 66078903 ID=MsG0380015166.01;Name=MsG0380015166.01
Chr3 mRNA 66078903 66079946 66078903 ID=MsG0380015166.01.T01;Parent=MsG0380015166.01;Name=MsG0380015166.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|3|0|82
Chr3 exon 66078903 66078947 66078903 ID=MsG0380015166.01.T01:exon:13278;Parent=MsG0380015166.01.T01
Chr3 exon 66079626 66079730 66079626 ID=MsG0380015166.01.T01:exon:13279;Parent=MsG0380015166.01.T01
Chr3 exon 66079848 66079946 66079848 ID=MsG0380015166.01.T01:exon:13280;Parent=MsG0380015166.01.T01
Chr3 CDS 66078903 66078947 66078903 ID=MsG0380015166.01.T01:cds;Parent=MsG0380015166.01.T01
Chr3 CDS 66079626 66079730 66079626 ID=MsG0380015166.01.T01:cds;Parent=MsG0380015166.01.T01
Chr3 CDS 66079848 66079946 66079848 ID=MsG0380015166.01.T01:cds;Parent=MsG0380015166.01.T01
Gene Sequence

>MsG0380015166.01.T01

ATGCATCCTCCTTTAACATTGCACAAACATCCTATGTGCGCTGAAATTATCGAGGAGTTTCAAAAGTGTCATGTGGAACATCCTATTGCAAAATTCTTTGGCGAATGTACAGAATTGAAAATAAAATTGGATCGCTGCTTCAGAGCAGAAAAAGCTGTGAAGAGAAAGGCCAACTTTGAAAAGAGCAAAGAACTCAAGGAACAATTACGAGTTTTAAGGAAAGAAAATGCTGCAAGCAACAGTCAATAG

Protein sequence

>MsG0380015166.01.T01

MHPPLTLHKHPMCAEIIEEFQKCHVEHPIAKFFGECTELKIKLDRCFRAEKAVKRKANFEKSKELKEQLRVLRKENAASNSQ*