AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021768.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021768.01.T01 MTR_4g076090 97.059 68 2 0 1 68 1 68 1.81e-44 137
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021768.01.T01 AT3G22210 62.500 64 23 1 5 68 6 68 1.55e-20 77.0

Find 13 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATTCGCTCTTCCAGTTT+TGG 0.231911 4:+64048140 MsG0480021768.01.T01:CDS
GTCTTCTAGCTCGCCTCTCT+CGG 0.346161 4:-64048370 MsG0480021767.01.T01:intron
GACAGAAATCACTTCAAGAT+TGG 0.349085 4:+64048300 MsG0480021768.01.T01:intron
GCTGCAACAATGCCCAAAAC+TGG 0.365881 4:-64048153 MsG0480021767.01.T01:intron
AGCTCGCCTCTCTCGGGAGC+TGG 0.374844 4:-64048363 MsG0480021767.01.T01:intron
CTCTCTCGGGAGCTGGATGT+TGG 0.452110 4:-64048356 MsG0480021767.01.T01:intron
ACAGAAATCACTTCAAGATT+GGG 0.482353 4:+64048301 MsG0480021768.01.T01:intron
ATTGAAACTCACTGCATAGA+AGG 0.485260 4:-64048187 MsG0480021767.01.T01:intron
TCTTCTAGCTCGCCTCTCTC+GGG 0.500761 4:-64048369 MsG0480021767.01.T01:intron
GATGATTCTGAATCAGAGAA+TGG 0.538128 4:+64048323 MsG0480021768.01.T01:CDS
CAACATCCAGCTCCCGAGAG+AGG 0.612999 4:+64048357 MsG0480021768.01.T01:CDS
GAGTTTCAATGAGATTAGAG+AGG 0.641117 4:+64048200 MsG0480021768.01.T01:CDS
GATTCTGAATCAGAGAATGG+AGG 0.645594 4:+64048326 MsG0480021768.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAATCACTTCAAGATT+GGG + Chr4:64048301-64048320 MsG0480021768.01.T01:intron 30.0%
ATTGAAACTCACTGCATAGA+AGG - Chr4:64048190-64048209 MsG0480021767.01.T01:intron 35.0%
GACAGAAATCACTTCAAGAT+TGG + Chr4:64048300-64048319 MsG0480021768.01.T01:intron 35.0%
GAGTTTCAATGAGATTAGAG+AGG + Chr4:64048200-64048219 MsG0480021768.01.T01:CDS 35.0%
GATGATTCTGAATCAGAGAA+TGG + Chr4:64048323-64048342 MsG0480021768.01.T01:CDS 35.0%
GATTCTGAATCAGAGAATGG+AGG + Chr4:64048326-64048345 MsG0480021768.01.T01:CDS 40.0%
! CAATTCGCTCTTCCAGTTTT+GGG + Chr4:64048141-64048160 MsG0480021768.01.T01:CDS 40.0%
! GCAATTCGCTCTTCCAGTTT+TGG + Chr4:64048140-64048159 MsG0480021768.01.T01:CDS 45.0%
GCTGCAACAATGCCCAAAAC+TGG - Chr4:64048156-64048175 MsG0480021767.01.T01:intron 50.0%
!! GTCTTCTAGCTCGCCTCTCT+CGG - Chr4:64048373-64048392 MsG0480021767.01.T01:intron 55.0%
!! TCTTCTAGCTCGCCTCTCTC+GGG - Chr4:64048372-64048391 MsG0480021767.01.T01:intron 55.0%
CAACATCCAGCTCCCGAGAG+AGG + Chr4:64048357-64048376 MsG0480021768.01.T01:CDS 60.0%
CTCTCTCGGGAGCTGGATGT+TGG - Chr4:64048359-64048378 MsG0480021767.01.T01:intron 60.0%
AGCTCGCCTCTCTCGGGAGC+TGG - Chr4:64048366-64048385 MsG0480021767.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 64048129 64048418 64048129 ID=MsG0480021768.01;Name=MsG0480021768.01
Chr4 mRNA 64048129 64048418 64048129 ID=MsG0480021768.01.T01;Parent=MsG0480021768.01;Name=MsG0480021768.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|0|0|2|0|68
Chr4 exon 64048129 64048221 64048129 ID=MsG0480021768.01.T01:exon:58494;Parent=MsG0480021768.01.T01
Chr4 exon 64048305 64048418 64048305 ID=MsG0480021768.01.T01:exon:58495;Parent=MsG0480021768.01.T01
Chr4 CDS 64048129 64048221 64048129 ID=MsG0480021768.01.T01:cds;Parent=MsG0480021768.01.T01
Chr4 CDS 64048305 64048418 64048305 ID=MsG0480021768.01.T01:cds;Parent=MsG0480021768.01.T01
Gene Sequence

>MsG0480021768.01.T01

ATGGATGCAATGCAATTCGCTCTTCCAGTTTTGGGCATTGTTGCAGCTGCAGCTGTGACCTTCTATGCAGTGAGTTTCAATGAGATTAGAGAGAAATCACTTCAAGATTGGGATGATTCTGAATCAGAGAATGGAGGTTTTAGACCAACATCCAGCTCCCGAGAGAGGCGAGCTAGAAGACAAGCTAGTAAAAATACCAAAAAATAA

Protein sequence

>MsG0480021768.01.T01

MDAMQFALPVLGIVAAAAVTFYAVSFNEIREKSLQDWDDSESENGGFRPTSSSRERRARRQASKNTKK*