AlfalfaGEDB | MsG0180003173.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003173.01

KEGG: -
GO: -
PFAM: PF07734

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003173.01.T01	XP_013467794.1	75.472	371	40	3	2	321	5	375	0	536

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003173.01.T01	Q9SU30	27.011	348	177	17	10	287	4	344	6.70E-16	81.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003173.01.T01	A0A072VJR2	75.472	371	40	3	2	321	5	375	0.0	536

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0180003173.01	0.808853	2.525467e-50	1.459730e-47
MsG0180001312.01	MsG0180003173.01	0.809744	1.626135e-50	9.626159e-48
MsG0180002952.01	MsG0180003173.01	0.816512	5.323893e-52	3.779903e-49
MsG0180003173.01	MsG0180003810.01	0.814829	1.262474e-51	8.560727e-49
MsG0180003173.01	MsG0180005185.01	0.805434	1.337718e-49	7.076453e-47
MsG0180003173.01	MsG0280009748.01	0.810695	1.014211e-50	6.155820e-48
MsG0180003173.01	MsG0280010816.01	0.814120	1.811253e-51	1.204879e-48
MsG0180003173.01	MsG0280011334.01	0.807497	4.911649e-50	2.739960e-47
MsG0180003173.01	MsG0380013834.01	0.809589	1.756403e-50	1.035422e-47
MsG0180003173.01	MsG0380014701.01	0.803303	3.717794e-49	1.861736e-46
MsG0180003173.01	MsG0480022259.01	0.802329	5.908773e-49	2.886498e-46
MsG0180003173.01	MsG0480022827.01	0.800181	1.626070e-48	7.521942e-46
MsG0180003173.01	MsG0580024304.01	0.818552	1.846452e-52	1.386519e-49
MsG0180003173.01	MsG0580024632.01	0.820153	7.972670e-53	6.259082e-50
MsG0180003173.01	MsG0680032801.01	0.804283	2.327217e-49	1.195127e-46
MsG0180003173.01	MsG0780036773.01	0.812029	5.207555e-51	3.274434e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003173.01.T01	MTR_1g055135	75.472	371	40	3	2	321	5	375	0.0	536
MsG0180003173.01.T01	MTR_5g097210	53.073	358	117	8	7	317	6	359	1.29e-111	328
MsG0180003173.01.T01	MTR_1g055165	50.811	370	81	5	1	321	1	318	6.32e-110	322
MsG0180003173.01.T01	MTR_4g117750	52.500	360	120	7	9	320	2	358	1.10e-98	299
MsG0180003173.01.T01	MTR_4g117750	47.368	133	58	4	198	319	350	481	2.12e-26	108
MsG0180003173.01.T01	MTR_1g055175	71.503	193	54	1	130	321	9	201	2.52e-95	281
MsG0180003173.01.T01	MTR_1g055195	59.701	268	89	7	60	321	224	478	8.92e-94	287
MsG0180003173.01.T01	MTR_3g103020	48.780	369	124	10	9	321	19	378	1.02e-92	281
MsG0180003173.01.T01	MTR_4g118370	58.776	245	81	4	74	318	33	257	2.59e-91	273
MsG0180003173.01.T01	MTR_4g117850	48.324	358	131	9	7	314	20	373	7.91e-91	276
MsG0180003173.01.T01	MTR_4g117720	52.059	340	125	12	9	321	50	378	2.83e-90	274
MsG0180003173.01.T01	MTR_1g055250	53.177	299	71	7	5	300	16	248	4.52e-89	270
MsG0180003173.01.T01	MTR_4g109280	47.514	362	132	13	9	321	7	359	4.27e-84	258
MsG0180003173.01.T01	MTR_6g060650	54.851	268	106	8	60	320	113	372	3.30e-83	256
MsG0180003173.01.T01	MTR_5g070130	44.624	372	143	11	9	321	10	377	5.48e-83	256
MsG0180003173.01.T01	MTR_3g034180	44.327	379	132	12	9	320	122	488	3.83e-82	257
MsG0180003173.01.T01	MTR_3g034160	44.267	375	138	10	9	320	8	374	3.25e-79	246
MsG0180003173.01.T01	MTR_4g023130	49.387	326	130	10	9	320	8	312	5.30e-79	243
MsG0180003173.01.T01	MTR_4g116330	42.257	381	144	12	2	321	15	380	4.00e-78	243
MsG0180003173.01.T01	MTR_3g034130	44.798	346	128	8	9	294	8	350	7.19e-78	242
MsG0180003173.01.T01	MTR_4g122850	42.577	357	145	9	21	321	12	364	1.50e-76	239
MsG0180003173.01.T01	MTR_4g023090	40.979	388	138	11	8	321	14	384	4.95e-75	236
MsG0180003173.01.T01	MTR_4g109270	44.205	371	134	12	10	321	8	364	9.04e-75	234
MsG0180003173.01.T01	MTR_5g070080	51.481	270	115	9	60	321	125	386	1.14e-74	234
MsG0180003173.01.T01	MTR_4g023070	42.819	376	141	11	10	320	3	369	1.73e-74	234
MsG0180003173.01.T01	MTR_4g021785	43.750	368	132	10	9	321	8	355	1.98e-74	233
MsG0180003173.01.T01	MTR_5g070088	49.817	273	118	9	60	321	122	386	1.22e-72	229
MsG0180003173.01.T01	MTR_4g117780	41.755	376	142	11	6	320	7	366	3.09e-72	228
MsG0180003173.01.T01	MTR_4g023080	43.869	367	130	10	9	320	8	353	3.90e-72	227
MsG0180003173.01.T01	MTR_8g032390	44.828	348	152	11	1	316	31	370	3.61e-71	225
MsG0180003173.01.T01	MTR_8g433040	41.600	375	146	11	1	321	9	364	5.10e-71	224
MsG0180003173.01.T01	MTR_8g032380	41.600	375	146	11	1	321	9	364	5.10e-71	224
MsG0180003173.01.T01	MTR_4g117770	42.588	371	148	10	6	321	7	367	5.51e-70	222
MsG0180003173.01.T01	MTR_4g117810	42.588	371	148	10	6	321	7	367	5.51e-70	222
MsG0180003173.01.T01	MTR_4g023120	41.425	379	144	12	10	321	3	370	1.51e-69	221
MsG0180003173.01.T01	MTR_4g094365	41.600	375	140	12	9	321	7	364	3.59e-69	220
MsG0180003173.01.T01	MTR_4g118380	52.191	251	93	10	60	292	115	356	1.22e-67	216
MsG0180003173.01.T01	MTR_4g023380	38.734	395	128	10	8	320	14	376	9.14e-66	211
MsG0180003173.01.T01	MTR_4g117870	44.528	265	103	8	60	321	44	267	9.87e-61	195
MsG0180003173.01.T01	MTR_8g433030	44.860	321	158	10	1	316	31	337	1.34e-60	197
MsG0180003173.01.T01	MTR_7g079400	42.593	270	144	6	60	321	71	337	7.09e-58	190
MsG0180003173.01.T01	MTR_4g023190	39.108	381	146	13	1	319	1	357	5.23e-56	186
MsG0180003173.01.T01	MTR_4g023150	38.482	382	148	13	1	319	1	358	1.25e-55	184
MsG0180003173.01.T01	MTR_4g116320	50.691	217	101	4	104	318	43	255	3.94e-55	180
MsG0180003173.01.T01	MTR_4g116330	37.008	381	125	13	2	321	15	341	4.08e-55	183
MsG0180003173.01.T01	MTR_4g023200	38.484	343	125	12	39	319	40	358	1.23e-54	182
MsG0180003173.01.T01	MTR_4g023440	37.940	369	120	10	8	320	16	331	2.38e-54	181
MsG0180003173.01.T01	MTR_7g079370	43.750	272	135	8	60	316	83	351	5.65e-53	178
MsG0180003173.01.T01	MTR_8g102520	36.607	336	170	8	9	321	7	322	9.90e-52	173
MsG0180003173.01.T01	MTR_4g118400	44.615	260	106	11	60	315	69	294	2.36e-50	169
MsG0180003173.01.T01	MTR_4g121580	38.286	350	118	14	2	287	3	318	3.04e-48	166
MsG0180003173.01.T01	MTR_8g040935	45.673	208	102	7	121	321	5	208	3.71e-44	150
MsG0180003173.01.T01	MTR_8g013010	40.590	271	90	10	9	222	53	309	2.46e-42	149
MsG0180003173.01.T01	MTR_2g048580	34.417	369	153	18	9	321	7	342	1.08e-41	148
MsG0180003173.01.T01	MTR_7g079420	37.261	314	128	12	26	321	882	1144	2.73e-40	150
MsG0180003173.01.T01	MTR_4g118360	55.118	127	53	3	198	321	1	126	1.24e-37	130
MsG0180003173.01.T01	MTR_4g117530	33.742	326	117	11	5	321	18	253	3.43e-37	134
MsG0180003173.01.T01	MTR_6g463430	41.627	209	113	6	115	321	1	202	5.31e-37	131
MsG0180003173.01.T01	MTR_4g114700	37.175	269	104	10	56	312	3	218	1.53e-34	126
MsG0180003173.01.T01	MTR_4g122860	34.154	325	129	14	8	320	68	319	5.23e-34	127
MsG0180003173.01.T01	MTR_4g116340	39.381	226	92	8	2	188	28	247	4.44e-32	120
MsG0180003173.01.T01	MTR_4g117430	42.262	168	72	4	62	222	3	152	7.82e-31	114
MsG0180003173.01.T01	MTR_1g055200	60.714	84	33	0	55	138	2	85	7.26e-26	99.8
MsG0180003173.01.T01	MTR_7g079450	43.478	138	75	2	184	321	3	137	8.90e-25	97.8
MsG0180003173.01.T01	MTR_5g023000	55.789	95	39	2	229	321	6	99	1.17e-24	96.3
MsG0180003173.01.T01	MTR_8g098830	38.750	160	94	3	132	289	37	194	6.12e-24	98.2
MsG0180003173.01.T01	MTR_1g055145	62.963	81	29	1	158	238	123	202	3.38e-23	97.4
MsG0180003173.01.T01	MTR_4g133170	29.741	232	134	8	60	271	91	313	9.70e-21	92.0
MsG0180003173.01.T01	MTR_1g068875	28.814	295	149	10	10	251	4	290	4.22e-20	90.1
MsG0180003173.01.T01	MTR_1g094210	27.350	351	159	16	7	289	54	376	1.95e-19	88.6
MsG0180003173.01.T01	MTR_1g094210	27.321	377	176	17	5	313	52	398	3.31e-19	87.8
MsG0180003173.01.T01	MTR_1g068835	28.378	296	150	11	10	251	4	291	4.29e-19	87.4
MsG0180003173.01.T01	MTR_4g061340	28.438	320	140	15	6	259	22	318	2.95e-18	84.7
MsG0180003173.01.T01	MTR_7g117720	37.640	178	59	8	9	143	9	177	5.96e-18	82.8
MsG0180003173.01.T01	MTR_7g117720	43.333	120	63	3	202	316	153	272	7.05e-18	82.4
MsG0180003173.01.T01	MTR_4g109290	44.882	127	49	5	8	113	7	133	8.75e-18	80.5
MsG0180003173.01.T01	MTR_2g026010	30.476	210	125	7	60	251	91	297	1.42e-17	83.2
MsG0180003173.01.T01	MTR_2g021050	31.429	210	120	7	94	287	126	327	4.54e-17	81.6
MsG0180003173.01.T01	MTR_2g031680	26.349	315	169	9	10	269	6	312	8.64e-17	80.9
MsG0180003173.01.T01	MTR_2g028140	29.583	240	134	8	71	288	129	355	1.11e-16	80.5
MsG0180003173.01.T01	MTR_2g028130	29.949	197	115	5	70	251	127	315	1.97e-16	79.7
MsG0180003173.01.T01	MTR_3g007330	28.339	307	149	12	6	260	44	331	3.93e-16	79.0
MsG0180003173.01.T01	MTR_6g065980	26.645	304	132	13	4	247	10	282	5.41e-16	78.2
MsG0180003173.01.T01	MTR_2g021190	27.907	301	141	12	8	247	19	304	7.55e-16	77.8
MsG0180003173.01.T01	MTR_2g024100	30.457	197	109	5	71	247	118	306	9.76e-16	77.8
MsG0180003173.01.T01	MTR_3g023930	27.055	292	132	12	8	247	32	294	1.02e-15	77.4
MsG0180003173.01.T01	MTR_6g034720	44.231	104	52	2	99	201	73	171	1.22e-15	75.1
MsG0180003173.01.T01	MTR_2g426020	26.136	352	176	14	10	288	6	346	2.31e-15	76.6
MsG0180003173.01.T01	MTR_2g025420	29.146	199	111	6	75	251	132	322	2.53e-15	76.6
MsG0180003173.01.T01	MTR_1g068860	27.885	208	128	5	60	251	92	293	3.02e-15	76.3
MsG0180003173.01.T01	MTR_3g014570	28.527	319	146	15	1	258	20	317	3.81e-15	75.9
MsG0180003173.01.T01	MTR_2g031640	28.774	212	122	8	60	251	93	295	4.13e-15	75.9
MsG0180003173.01.T01	MTR_5g069120	47.253	91	36	3	60	138	87	177	4.42e-15	72.4
MsG0180003173.01.T01	MTR_6g065510	25.839	298	156	13	9	271	22	289	5.58e-15	75.1
MsG0180003173.01.T01	MTR_3g036290	28.526	312	121	16	6	251	18	293	6.23e-15	75.1
MsG0180003173.01.T01	MTR_2g028160	28.700	223	136	7	71	275	128	345	6.43e-15	75.5
MsG0180003173.01.T01	MTR_2g078900	25.839	298	148	10	6	247	29	309	9.47e-15	74.3
MsG0180003173.01.T01	MTR_5g097680	28.571	224	121	9	68	256	152	371	1.12e-14	74.7
MsG0180003173.01.T01	MTR_1g050542	24.742	291	157	10	5	246	53	330	1.33e-14	74.3
MsG0180003173.01.T01	MTR_2g031720	27.303	304	149	15	10	251	6	299	1.67e-14	73.9
MsG0180003173.01.T01	MTR_2g025960	28.856	201	111	7	71	251	27	215	2.19e-14	72.8
MsG0180003173.01.T01	MTR_4g070550	26.498	317	144	12	10	247	5	311	2.37e-14	73.6
MsG0180003173.01.T01	MTR_3g006670	27.869	305	144	13	4	247	43	332	2.76e-14	73.6
MsG0180003173.01.T01	MTR_2g426070	27.945	365	168	16	1	288	1	347	4.50e-14	72.8
MsG0180003173.01.T01	MTR_8g013005	54.839	62	23	2	262	321	1	59	5.30e-14	66.2
MsG0180003173.01.T01	MTR_2g024110	24.917	301	158	10	10	252	4	294	6.06e-14	72.4
MsG0180003173.01.T01	MTR_0667s0010	28.856	201	113	7	71	251	116	306	6.71e-14	72.4
MsG0180003173.01.T01	MTR_3g010590	25.526	380	185	15	6	313	32	385	9.53e-14	71.6
MsG0180003173.01.T01	MTR_3g083660	29.183	257	115	12	6	259	21	213	9.97e-14	70.9
MsG0180003173.01.T01	MTR_3g011100	28.125	320	142	16	6	259	231	528	1.08e-13	72.0
MsG0180003173.01.T01	MTR_4g023430	45.122	82	41	2	244	321	141	222	1.59e-13	69.3
MsG0180003173.01.T01	MTR_4g107820	26.578	301	143	13	10	251	36	317	1.80e-13	70.9
MsG0180003173.01.T01	MTR_4g107780	26.578	301	143	13	10	251	36	317	1.80e-13	70.9
MsG0180003173.01.T01	MTR_4g107780	26.578	301	143	13	10	251	36	317	2.32e-13	70.5
MsG0180003173.01.T01	MTR_4g055060	25.714	315	148	14	5	251	25	321	2.33e-13	70.5
MsG0180003173.01.T01	MTR_4g107820	26.578	301	143	13	10	251	36	317	2.39e-13	70.5
MsG0180003173.01.T01	MTR_3g009310	26.861	309	139	12	8	251	40	326	2.87e-13	70.5
MsG0180003173.01.T01	MTR_3g010390	27.976	336	147	17	5	260	27	347	2.96e-13	70.1
MsG0180003173.01.T01	MTR_5g057790	27.213	305	139	13	10	251	46	330	3.55e-13	70.1
MsG0180003173.01.T01	MTR_2g078870	27.461	193	117	8	60	247	58	232	4.29e-13	69.3
MsG0180003173.01.T01	MTR_5g057710	26.974	304	141	12	10	251	46	330	4.49e-13	69.7
MsG0180003173.01.T01	MTR_3g083010	27.615	239	142	7	73	288	132	362	5.53e-13	69.7
MsG0180003173.01.T01	MTR_0044s0300	26.910	301	138	14	8	247	36	315	5.55e-13	69.3
MsG0180003173.01.T01	MTR_3g010430	26.471	306	142	12	6	247	32	318	6.06e-13	69.3
MsG0180003173.01.T01	MTR_7g013590	28.197	305	148	13	13	258	39	331	7.08e-13	69.3
MsG0180003173.01.T01	MTR_6g090180	27.068	266	149	11	50	285	149	399	7.17e-13	69.3
MsG0180003173.01.T01	MTR_3g010520	25.170	294	136	12	8	247	23	286	7.18e-13	68.9
MsG0180003173.01.T01	MTR_8g054320	31.034	261	129	11	8	263	30	244	1.62e-12	67.4
MsG0180003173.01.T01	MTR_2g046900	25.890	309	159	10	8	256	26	324	1.88e-12	67.8
MsG0180003173.01.T01	MTR_0054s0090	27.642	246	131	10	73	285	136	367	2.17e-12	67.8
MsG0180003173.01.T01	MTR_7g060960	28.618	304	149	14	7	263	29	311	2.51e-12	67.4
MsG0180003173.01.T01	MTR_8g099460	29.070	258	141	11	53	279	87	333	3.30e-12	67.0
MsG0180003173.01.T01	MTR_3g036430	26.211	351	188	17	8	313	18	342	4.55e-12	66.2
MsG0180003173.01.T01	MTR_3g007360	27.353	340	151	18	13	278	55	372	4.84e-12	66.6
MsG0180003173.01.T01	MTR_2g425980	29.630	243	136	9	71	287	120	353	4.92e-12	66.6
MsG0180003173.01.T01	MTR_4g006270	26.667	300	135	14	10	252	4	275	5.42e-12	66.6
MsG0180003173.01.T01	MTR_5g007610	28.571	322	141	16	8	260	39	340	5.98e-12	66.2
MsG0180003173.01.T01	MTR_2g021155	30.769	260	120	15	5	247	666	882	6.45e-12	67.0
MsG0180003173.01.T01	MTR_7g069550	27.652	264	147	13	66	298	131	381	6.80e-12	66.2
MsG0180003173.01.T01	MTR_0760s0010	27.365	296	125	14	15	248	52	319	7.10e-12	66.2
MsG0180003173.01.T01	MTR_3g007410	27.358	318	136	16	13	258	55	349	9.80e-12	65.9
MsG0180003173.01.T01	MTR_8g064890	29.091	220	122	11	71	270	136	341	1.07e-11	65.5
MsG0180003173.01.T01	MTR_5g043605	26.357	258	161	11	9	247	24	271	1.54e-11	65.1
MsG0180003173.01.T01	MTR_6g004660	26.667	315	150	14	10	255	16	318	2.45e-11	64.3
MsG0180003173.01.T01	MTR_3g024130	27.897	233	135	9	40	259	41	253	2.93e-11	63.9
MsG0180003173.01.T01	MTR_7g057270	25.982	331	150	14	10	257	26	344	2.99e-11	64.3
MsG0180003173.01.T01	MTR_0054s0100	26.718	262	142	11	60	285	122	369	5.77e-11	63.5
MsG0180003173.01.T01	MTR_6g007150	27.273	198	126	8	60	247	72	261	6.07e-11	63.2
MsG0180003173.01.T01	MTR_2g104340	21.859	398	217	14	1	314	5	392	6.45e-11	63.2
MsG0180003173.01.T01	MTR_3g007270	26.699	206	122	7	60	251	153	343	6.78e-11	63.2
MsG0180003173.01.T01	MTR_1004s0010	30.808	198	105	11	58	248	117	289	8.67e-11	62.8
MsG0180003173.01.T01	MTR_4g023100	46.753	77	34	2	244	320	49	118	9.27e-11	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003173.01.T01	AT4G12560	27.011	348	177	17	10	287	4	344	6.83e-17	81.3
MsG0180003173.01.T01	AT4G12560	27.011	348	177	17	10	287	4	344	6.83e-17	81.3
MsG0180003173.01.T01	AT4G12560	27.011	348	177	17	10	287	4	344	6.83e-17	81.3
MsG0180003173.01.T01	AT3G06240	29.615	260	142	12	73	313	182	419	9.87e-16	77.8

Find 71 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTGTTGCCTTTCATTTAA+TGG	0.113478	1:+57942606	MsG0180003173.01.T01:CDS
GTTCGACAGATTGTAATTTA+TGG	0.146258	1:+57942674	MsG0180003173.01.T01:CDS
CGTTTAAATTTCTATAGTTT+TGG	0.179921	1:+57942337	MsG0180003173.01.T01:CDS
TGGTAACCTTAATAAGATTT+TGG	0.210360	1:-57941965	None:intergenic
ACGAGGATAAAGTTGATATA+TGG	0.231752	1:+57942734	MsG0180003173.01.T01:CDS
TGATCACCAAAATCTTATTA+AGG	0.243263	1:+57941959	MsG0180003173.01.T01:CDS
AAATTTAAACGTGAATAATT+AGG	0.271228	1:-57942325	None:intergenic
TTAGAGGATTTCTCAGTCTA+TGG	0.277856	1:+57942701	MsG0180003173.01.T01:CDS
AAAGATTCAGAACATGGATT+TGG	0.297814	1:+57942940	MsG0180003173.01.T01:CDS
TTCGACAGATTGTAATTTAT+GGG	0.302194	1:+57942675	MsG0180003173.01.T01:CDS
CTCCCGGATGATATTGAATA+TGG	0.311329	1:+57942652	MsG0180003173.01.T01:CDS
GGATAAAGCTCACTATAAAA+AGG	0.347013	1:-57942502	None:intergenic
TTCTCAGTCTATGGGTTATG+AGG	0.357912	1:+57942710	MsG0180003173.01.T01:CDS
TGTAATTTATGGGTATTTAG+AGG	0.380678	1:+57942685	MsG0180003173.01.T01:CDS
GTGGTAGAGTCCGTCTTCAA+TGG	0.384732	1:+57942538	MsG0180003173.01.T01:CDS
TGGTAGAGTCCGTCTTCAAT+GGG	0.385463	1:+57942539	MsG0180003173.01.T01:CDS
CGGAAGACGCATGGCGGAGG+TGG	0.401862	1:+57941909	None:intergenic
GATAGCACATCATCTGAGTT+TGG	0.409606	1:-57942415	None:intergenic
GAAACGATAAGAGACTTAAC+TGG	0.420711	1:-57941985	None:intergenic
CGAGGATAAAGTTGATATAT+GGG	0.422656	1:+57942735	MsG0180003173.01.T01:CDS
TCTTCAATGGGGCTATTCAC+TGG	0.429378	1:+57942551	MsG0180003173.01.T01:CDS
AATACAAAGTGAAGTCATCT+TGG	0.433399	1:+57942767	MsG0180003173.01.T01:CDS
TATGAGGGAAATGTAAGAAA+TGG	0.441603	1:-57942818	None:intergenic
CTGAGTTTGGAATACAATCA+TGG	0.451583	1:-57942402	None:intergenic
TTCAGAACATGGATTTGGAT+TGG	0.464764	1:+57942945	MsG0180003173.01.T01:CDS
GAACCATATTCAATATCATC+CGG	0.477125	1:-57942655	None:intergenic
ATCAATTCATCGGGCAGATA+CGG	0.477260	1:-57941940	None:intergenic
CGAGTCAAGAGATGATTACT+TGG	0.482225	1:+57942366	MsG0180003173.01.T01:CDS
CAATGGGGCTATTCACTGGT+TGG	0.483990	1:+57942555	MsG0180003173.01.T01:CDS
TCAAATCCGTTTGTAAGTCA+TGG	0.484548	1:+57942006	MsG0180003173.01.T01:CDS
TACAATGATCGTTATGCTCT+AGG	0.488536	1:-57942918	None:intergenic
TTGCCTTTCATTTAATGGAA+AGG	0.488920	1:+57942611	MsG0180003173.01.T01:CDS
ACCTCTAGAAATTCCTCTCC+CGG	0.490524	1:+57942636	MsG0180003173.01.T01:CDS
TAGAGGATTTCTCAGTCTAT+GGG	0.497173	1:+57942702	MsG0180003173.01.T01:CDS
TTAAGCTTGATCAGTGTCAC+TGG	0.498029	1:-57942998	None:intergenic
CATTGTAAAGATTCAGAACA+TGG	0.498461	1:+57942934	MsG0180003173.01.T01:CDS
ATGATGTGCTATCTCGCATT+GGG	0.506359	1:+57942425	MsG0180003173.01.T01:CDS
TATCCTTTCCATTAAATGAA+AGG	0.507802	1:-57942614	None:intergenic
CAGCGGAAGACGCATGGCGG+AGG	0.508658	1:+57941906	None:intergenic
GGTGATATTGTTGGAACAGA+TGG	0.517456	1:+57942856	MsG0180003173.01.T01:CDS
GTTGGAACAGATGGATGTAC+TGG	0.522283	1:+57942865	MsG0180003173.01.T01:CDS
TCTACGAGTGTGTGTTGAAG+AGG	0.539919	1:-57942076	None:intergenic
TCTACCACAGAATAAGCAAA+AGG	0.540037	1:-57942523	None:intergenic
GATGATGTGCTATCTCGCAT+TGG	0.540292	1:+57942424	MsG0180003173.01.T01:CDS
AGAAAACCATGACTTACAAA+CGG	0.549180	1:-57942012	None:intergenic
TGGTGAGATATGATGACGAA+GGG	0.551766	1:+57942890	MsG0180003173.01.T01:CDS
TTTAAACGTGAATAATTAGG+AGG	0.552430	1:-57942322	None:intergenic
TGAGTTTGGAATACAATCAT+GGG	0.556899	1:-57942401	None:intergenic
AAACGTGAATAATTAGGAGG+AGG	0.557127	1:-57942319	None:intergenic
TAAGCTTGATCAGTGTCACT+GGG	0.557734	1:-57942997	None:intergenic
AAAGTTATAGGTTCATGTAG+AGG	0.565302	1:+57942220	MsG0180003173.01.T01:intron
AGTCTATGAACTCCGGTGGA+TGG	0.579322	1:-57942286	None:intergenic
TCTCAGTCTATGGGTTATGA+GGG	0.582388	1:+57942711	MsG0180003173.01.T01:CDS
TTTCAATGAGAGCTAATGTG+TGG	0.592234	1:+57942452	MsG0180003173.01.T01:CDS
AACCATATTCAATATCATCC+GGG	0.594830	1:-57942654	None:intergenic
TATTCAATATCATCCGGGAG+AGG	0.596373	1:-57942649	None:intergenic
TACCTATGGAATCCATCCAC+CGG	0.599416	1:+57942274	MsG0180003173.01.T01:CDS
GAATAATTAGGAGGAGGCGA+AGG	0.607195	1:-57942313	None:intergenic
TCATCGGGCAGATACGGTGG+TGG	0.618606	1:-57941934	None:intergenic
GTGAATAGCCCCATTGAAGA+CGG	0.620896	1:-57942548	None:intergenic
TATTAGTCTATGAACTCCGG+TGG	0.622217	1:-57942290	None:intergenic
AGGTATTAGTCTATGAACTC+CGG	0.626341	1:-57942293	None:intergenic
AACAGATGGATGTACTGGAT+TGG	0.639843	1:+57942870	MsG0180003173.01.T01:CDS
CTCCGGTGGATGGATTCCAT+AGG	0.641792	1:-57942276	None:intergenic
TCCGGGAGAGGAATTTCTAG+AGG	0.654536	1:-57942637	None:intergenic
AATTCATCGGGCAGATACGG+TGG	0.663893	1:-57941937	None:intergenic
GGTGAGATATGATGACGAAG+GGG	0.666620	1:+57942891	MsG0180003173.01.T01:CDS
TTGGTGAGATATGATGACGA+AGG	0.671162	1:+57942889	MsG0180003173.01.T01:CDS
GGTAGAGTCCGTCTTCAATG+GGG	0.708990	1:+57942540	MsG0180003173.01.T01:CDS
TCTATGGGTTATGAGGGACG+AGG	0.722807	1:+57942717	MsG0180003173.01.T01:CDS
AAGCTTGATCAGTGTCACTG+GGG	0.734643	1:-57942996	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTTAAACGTGAATAATT+AGG	-	Chr1:57942328-57942347	None:intergenic	15.0%
!!!	GTTTAAATTTCTATAGTTTT+GGG	+	Chr1:57942338-57942357	MsG0180003173.01.T01:CDS	15.0%
!!	ATTTAGGAAGATCAAAATTA+GGG	-	Chr1:57942186-57942205	None:intergenic	20.0%
!!!	CGTTTAAATTTCTATAGTTT+TGG	+	Chr1:57942337-57942356	MsG0180003173.01.T01:CDS	20.0%
!	AAAATTAGGGTTCAATAGAA+AGG	-	Chr1:57942173-57942192	None:intergenic	25.0%
!	CATTTCGATCTTAAAGTTAT+AGG	+	Chr1:57942208-57942227	MsG0180003173.01.T01:intron	25.0%
!	CTATAAAAAGGTAAATCTGT+AGG	-	Chr1:57942493-57942512	None:intergenic	25.0%
!	GATTTAGGAAGATCAAAATT+AGG	-	Chr1:57942187-57942206	None:intergenic	25.0%
!	TATCCTTTCCATTAAATGAA+AGG	-	Chr1:57942617-57942636	None:intergenic	25.0%
!	TATTGTTGCCTTTCATTTAA+TGG	+	Chr1:57942606-57942625	MsG0180003173.01.T01:CDS	25.0%
!	TGATCACCAAAATCTTATTA+AGG	+	Chr1:57941959-57941978	MsG0180003173.01.T01:CDS	25.0%
!	TGTAATTTATGGGTATTTAG+AGG	+	Chr1:57942685-57942704	MsG0180003173.01.T01:CDS	25.0%
!	TTAAGATCGAAATGAGATTT+AGG	-	Chr1:57942202-57942221	None:intergenic	25.0%
!	TTCGACAGATTGTAATTTAT+GGG	+	Chr1:57942675-57942694	MsG0180003173.01.T01:CDS	25.0%
!	TTTAAACGTGAATAATTAGG+AGG	-	Chr1:57942325-57942344	None:intergenic	25.0%
!!	ACTTTTTGTACAACATATGA+GGG	-	Chr1:57942836-57942855	None:intergenic	25.0%
!!	TGGTAACCTTAATAAGATTT+TGG	-	Chr1:57941968-57941987	None:intergenic	25.0%
!!!	ATGTAGAGGTTTTATATTGT+TGG	+	Chr1:57942234-57942253	MsG0180003173.01.T01:CDS	25.0%
!!!	GTTTTTCAAACATTTACCTA+TGG	+	Chr1:57942260-57942279	MsG0180003173.01.T01:CDS	25.0%
	AAAGATTCAGAACATGGATT+TGG	+	Chr1:57942940-57942959	MsG0180003173.01.T01:CDS	30.0%
	AAAGTTATAGGTTCATGTAG+AGG	+	Chr1:57942220-57942239	MsG0180003173.01.T01:intron	30.0%
	AACCATATTCAATATCATCC+GGG	-	Chr1:57942657-57942676	None:intergenic	30.0%
	AATACAAAGTGAAGTCATCT+TGG	+	Chr1:57942767-57942786	MsG0180003173.01.T01:CDS	30.0%
	ACAAAAAGTGGTGATATTGT+TGG	+	Chr1:57942847-57942866	MsG0180003173.01.T01:CDS	30.0%
	AGAAAACCATGACTTACAAA+CGG	-	Chr1:57942015-57942034	None:intergenic	30.0%
	CATTGTAAAGATTCAGAACA+TGG	+	Chr1:57942934-57942953	MsG0180003173.01.T01:CDS	30.0%
	CTCATATGTTGTACAAAAAG+TGG	+	Chr1:57942835-57942854	MsG0180003173.01.T01:CDS	30.0%
	GAACCATATTCAATATCATC+CGG	-	Chr1:57942658-57942677	None:intergenic	30.0%
	GGATAAAGCTCACTATAAAA+AGG	-	Chr1:57942505-57942524	None:intergenic	30.0%
	GTTCGACAGATTGTAATTTA+TGG	+	Chr1:57942674-57942693	MsG0180003173.01.T01:CDS	30.0%
	TATGAGGGAAATGTAAGAAA+TGG	-	Chr1:57942821-57942840	None:intergenic	30.0%
	TTGCCTTTCATTTAATGGAA+AGG	+	Chr1:57942611-57942630	MsG0180003173.01.T01:CDS	30.0%
!	TGAGTTTGGAATACAATCAT+GGG	-	Chr1:57942404-57942423	None:intergenic	30.0%
!	TTATCCTTTTGCTTATTCTG+TGG	+	Chr1:57942519-57942538	MsG0180003173.01.T01:CDS	30.0%
!!	ACGAGGATAAAGTTGATATA+TGG	+	Chr1:57942734-57942753	MsG0180003173.01.T01:CDS	30.0%
!!	ATTTTGGTGATCAATTCATC+GGG	-	Chr1:57941952-57941971	None:intergenic	30.0%
!!	CACTTTTTGTACAACATATG+AGG	-	Chr1:57942837-57942856	None:intergenic	30.0%
!!	CGAGGATAAAGTTGATATAT+GGG	+	Chr1:57942735-57942754	MsG0180003173.01.T01:CDS	30.0%
!!	GATTTTGGTGATCAATTCAT+CGG	-	Chr1:57941953-57941972	None:intergenic	30.0%
	AAACGTGAATAATTAGGAGG+AGG	-	Chr1:57942322-57942341	None:intergenic	35.0%
	CGTAGAATTCTCTTCATAAC+AGG	+	Chr1:57942093-57942112	MsG0180003173.01.T01:intron	35.0%
	GAAACGATAAGAGACTTAAC+TGG	-	Chr1:57941988-57942007	None:intergenic	35.0%
	TACAATGATCGTTATGCTCT+AGG	-	Chr1:57942921-57942940	None:intergenic	35.0%
	TAGAGGATTTCTCAGTCTAT+GGG	+	Chr1:57942702-57942721	MsG0180003173.01.T01:CDS	35.0%
	TCAAATCCGTTTGTAAGTCA+TGG	+	Chr1:57942006-57942025	MsG0180003173.01.T01:CDS	35.0%
	TCTACCACAGAATAAGCAAA+AGG	-	Chr1:57942526-57942545	None:intergenic	35.0%
	TTAGAGGATTTCTCAGTCTA+TGG	+	Chr1:57942701-57942720	MsG0180003173.01.T01:CDS	35.0%
	TTCAGAACATGGATTTGGAT+TGG	+	Chr1:57942945-57942964	MsG0180003173.01.T01:CDS	35.0%
	TTTCAATGAGAGCTAATGTG+TGG	+	Chr1:57942452-57942471	MsG0180003173.01.T01:CDS	35.0%
!	AGGTATTAGTCTATGAACTC+CGG	-	Chr1:57942296-57942315	None:intergenic	35.0%
!	CTGAGTTTGGAATACAATCA+TGG	-	Chr1:57942405-57942424	None:intergenic	35.0%
	AACAGATGGATGTACTGGAT+TGG	+	Chr1:57942870-57942889	MsG0180003173.01.T01:CDS	40.0%
	ATCAATTCATCGGGCAGATA+CGG	-	Chr1:57941943-57941962	None:intergenic	40.0%
	ATGATGTGCTATCTCGCATT+GGG	+	Chr1:57942425-57942444	MsG0180003173.01.T01:CDS	40.0%
	CGAGTCAAGAGATGATTACT+TGG	+	Chr1:57942366-57942385	MsG0180003173.01.T01:CDS	40.0%
	CTCCCGGATGATATTGAATA+TGG	+	Chr1:57942652-57942671	MsG0180003173.01.T01:CDS	40.0%
	GATAGCACATCATCTGAGTT+TGG	-	Chr1:57942418-57942437	None:intergenic	40.0%
	GGTGATATTGTTGGAACAGA+TGG	+	Chr1:57942856-57942875	MsG0180003173.01.T01:CDS	40.0%
	TATTCAATATCATCCGGGAG+AGG	-	Chr1:57942652-57942671	None:intergenic	40.0%
	TTAAGCTTGATCAGTGTCAC+TGG	-	Chr1:57943001-57943020	None:intergenic	40.0%
!	TAAGCTTGATCAGTGTCACT+GGG	-	Chr1:57943000-57943019	None:intergenic	40.0%
!	TATTAGTCTATGAACTCCGG+TGG	-	Chr1:57942293-57942312	None:intergenic	40.0%
!	TCTCAGTCTATGGGTTATGA+GGG	+	Chr1:57942711-57942730	MsG0180003173.01.T01:CDS	40.0%
!	TGGTGAGATATGATGACGAA+GGG	+	Chr1:57942890-57942909	MsG0180003173.01.T01:CDS	40.0%
!	TTCTCAGTCTATGGGTTATG+AGG	+	Chr1:57942710-57942729	MsG0180003173.01.T01:CDS	40.0%
!	TTGGTGAGATATGATGACGA+AGG	+	Chr1:57942889-57942908	MsG0180003173.01.T01:CDS	40.0%
	GAATAATTAGGAGGAGGCGA+AGG	-	Chr1:57942316-57942335	None:intergenic	45.0%
	GATGATGTGCTATCTCGCAT+TGG	+	Chr1:57942424-57942443	MsG0180003173.01.T01:CDS	45.0%
	GTGAATAGCCCCATTGAAGA+CGG	-	Chr1:57942551-57942570	None:intergenic	45.0%
	GTTGGAACAGATGGATGTAC+TGG	+	Chr1:57942865-57942884	MsG0180003173.01.T01:CDS	45.0%
	TACCTATGGAATCCATCCAC+CGG	+	Chr1:57942274-57942293	MsG0180003173.01.T01:CDS	45.0%
	TCTACGAGTGTGTGTTGAAG+AGG	-	Chr1:57942079-57942098	None:intergenic	45.0%
	TCTTCAATGGGGCTATTCAC+TGG	+	Chr1:57942551-57942570	MsG0180003173.01.T01:CDS	45.0%
	TGGTAGAGTCCGTCTTCAAT+GGG	+	Chr1:57942539-57942558	MsG0180003173.01.T01:CDS	45.0%
!	AAGCTTGATCAGTGTCACTG+GGG	-	Chr1:57942999-57943018	None:intergenic	45.0%
!	ACCTCTAGAAATTCCTCTCC+CGG	+	Chr1:57942636-57942655	MsG0180003173.01.T01:CDS	45.0%
!	GGTGAGATATGATGACGAAG+GGG	+	Chr1:57942891-57942910	MsG0180003173.01.T01:CDS	45.0%
	AATTCATCGGGCAGATACGG+TGG	-	Chr1:57941940-57941959	None:intergenic	50.0%
	AGTCTATGAACTCCGGTGGA+TGG	-	Chr1:57942289-57942308	None:intergenic	50.0%
	CAATGGGGCTATTCACTGGT+TGG	+	Chr1:57942555-57942574	MsG0180003173.01.T01:CDS	50.0%
	GGTAGAGTCCGTCTTCAATG+GGG	+	Chr1:57942540-57942559	MsG0180003173.01.T01:CDS	50.0%
	GTGGTAGAGTCCGTCTTCAA+TGG	+	Chr1:57942538-57942557	MsG0180003173.01.T01:CDS	50.0%
!	TCCGGGAGAGGAATTTCTAG+AGG	-	Chr1:57942640-57942659	None:intergenic	50.0%
!	TCTATGGGTTATGAGGGACG+AGG	+	Chr1:57942717-57942736	MsG0180003173.01.T01:CDS	50.0%
!	CTCCGGTGGATGGATTCCAT+AGG	-	Chr1:57942279-57942298	None:intergenic	55.0%
	TCATCGGGCAGATACGGTGG+TGG	-	Chr1:57941937-57941956	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	57941919	57943020	57941919	ID=MsG0180003173.01;Name=MsG0180003173.01
Chr1	mRNA	57941919	57943020	57941919	ID=MsG0180003173.01.T01;Parent=MsG0180003173.01;Name=MsG0180003173.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|321
Chr1	exon	57941919	57942093	57941919	ID=MsG0180003173.01.T01:exon:48871;Parent=MsG0180003173.01.T01
Chr1	exon	57942230	57943020	57942230	ID=MsG0180003173.01.T01:exon:48872;Parent=MsG0180003173.01.T01
Chr1	CDS	57941919	57942093	57941919	ID=MsG0180003173.01.T01:cds;Parent=MsG0180003173.01.T01
Chr1	CDS	57942230	57943020	57942230	ID=MsG0180003173.01.T01:cds;Parent=MsG0180003173.01.T01

Gene Sequence

>MsG0180003173.01.T01

ATGGCGGAGGTGGATCCACCACCGTATCTGCCCGATGAATTGATCACCAAAATCTTATTAAGGTTACCAGTTAAGTCTCTTATCGTTTCAAATCCGTTTGTAAGTCATGGTTTTCTTATCTCTGATTCTCACTTTGCAAATTCACATTTTCAACTTACCTCTTCAACACACACTCGTTCATGTAGAGGTTTTATATTGTTGGAGTGTTTTTCAAACATTTACCTATGGAATCCATCCACCGGAGTTCATAGACTAATACCTTCGCCTCCTCCTAATTATTCACGTTTAAATTTCTATAGTTTTGGGTATGACGAGTCAAGAGATGATTACTTGGTAGTTTTATTATCCCATGATTGTATTCCAAACTCAGATGATGTGCTATCTCGCATTGGGATTTTTTCAATGAGAGCTAATGTGTGGAACGAAATTGTGAGTCCTACAGATTTACCTTTTTATAGTGAGCTTTATCCTTTTGCTTATTCTGTGGTAGAGTCCGTCTTCAATGGGGCTATTCACTGGTTGGCTATTCGTCATGATACATATGAGTATGTTATTGTTGCCTTTCATTTAATGGAAAGGATACCTCTAGAAATTCCTCTCCCGGATGATATTGAATATGGTTCGACAGATTGTAATTTATGGGTATTTAGAGGATTTCTCAGTCTATGGGTTATGAGGGACGAGGATAAAGTTGATATATGGGTGATGAAAGAATACAAAGTGAAGTCATCTTGGACTAAAACTCTTGTTCTTGATTTGCATACCATTTCTTACATTTCCCTCATATGTTGTACAAAAAGTGGTGATATTGTTGGAACAGATGGATGTACTGGATTGGTGAGATATGATGACGAAGGGGAGTTCCTAGAGCATAACGATCATTGTAAAGATTCAGAACATGGATTTGGATTGGTTGTGTATACAGAGTCTCTGCTTTCACTCCCCAGTGACACTGATCAAGCTTAA

Protein sequence

>MsG0180003173.01.T01

MAEVDPPPYLPDELITKILLRLPVKSLIVSNPFVSHGFLISDSHFANSHFQLTSSTHTRSCRGFILLECFSNIYLWNPSTGVHRLIPSPPPNYSRLNFYSFGYDESRDDYLVVLLSHDCIPNSDDVLSRIGIFSMRANVWNEIVSPTDLPFYSELYPFAYSVVESVFNGAIHWLAIRHDTYEYVIVAFHLMERIPLEIPLPDDIEYGSTDCNLWVFRGFLSLWVMRDEDKVDIWVMKEYKVKSSWTKTLVLDLHTISYISLICCTKSGDIVGTDGCTGLVRYDDEGEFLEHNDHCKDSEHGFGLVVYTESLLSLPSDTDQA*