AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024632.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024632.01.T01 MTR_5g017820 90.984 122 10 1 1 122 1 121 1.47e-42 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024632.01.T01 AT5G66840 61.702 47 18 0 76 122 79 125 1.23e-13 66.2
MsG0580024632.01.T01 AT5G66840 61.702 47 18 0 76 122 79 125 1.23e-13 66.2

Find 16 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTTCACTAGTATCTGAT+TGG 0.348352 5:+7419033 None:intergenic
AAACATGGATTGAGGCTTGC+AGG 0.354847 5:-7418766 MsG0580024632.01.T01:CDS
AAAGTTGTTTCTCTCCTTCA+AGG 0.364185 5:-7418927 MsG0580024632.01.T01:intron
AGAAGATCGCGATGAAGCTT+CGG 0.439866 5:-7418991 MsG0580024632.01.T01:CDS
ATCTTCTTCTTCAGAGGAAG+AGG 0.453477 5:+7419112 None:intergenic
AATTTCACTAGTATCTGATT+GGG 0.467790 5:+7419034 None:intergenic
ACGAATGCAAAGCATATTTG+CGG 0.480322 5:-7418789 MsG0580024632.01.T01:CDS
GCAATCGCTGAGTTGTTCTT+CGG 0.504039 5:+7419067 None:intergenic
CTAGGATAAAAGAGCATTGG+AGG 0.517575 5:-7418723 MsG0580024632.01.T01:CDS
ATTTGCGGAAACATGGATTG+AGG 0.528841 5:-7418774 MsG0580024632.01.T01:CDS
TCCTCTGAAGAAGAAGATGA+TGG 0.533862 5:-7419107 MsG0580024632.01.T01:CDS
ACCATCATCTTCTTCTTCAG+AGG 0.537897 5:+7419106 None:intergenic
TTGCTAGGATAAAAGAGCAT+TGG 0.543652 5:-7418726 MsG0580024632.01.T01:CDS
ATAGAGCAGTTTGTATTGCT+AGG 0.554640 5:-7418741 MsG0580024632.01.T01:CDS
TACTAGTGAAATTGAAGATG+AGG 0.566868 5:-7419024 MsG0580024632.01.T01:CDS
AAAGCATATTTGCGGAAACA+TGG 0.596916 5:-7418781 MsG0580024632.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGTTAATTTTCAATTCATT+TGG + Chr5:7418983-7419002 None:intergenic 15.0%
! AATTTCACTAGTATCTGATT+GGG + Chr5:7418841-7418860 None:intergenic 25.0%
CAATTTCACTAGTATCTGAT+TGG + Chr5:7418842-7418861 None:intergenic 30.0%
GGTAGAAATAAAAACCTTGA+AGG + Chr5:7418962-7418981 None:intergenic 30.0%
TACTAGTGAAATTGAAGATG+AGG - Chr5:7418848-7418867 MsG0580024632.01.T01:intron 30.0%
! AAGGAGAGAAACAACTTTTT+CGG + Chr5:7418943-7418962 None:intergenic 30.0%
!! AATTTCGTGTTTGATTTTCC+AGG - Chr5:7419031-7419050 MsG0580024632.01.T01:CDS 30.0%
AAAGCATATTTGCGGAAACA+TGG - Chr5:7419091-7419110 MsG0580024632.01.T01:CDS 35.0%
ACGAATGCAAAGCATATTTG+CGG - Chr5:7419083-7419102 MsG0580024632.01.T01:CDS 35.0%
ATAGAGCAGTTTGTATTGCT+AGG - Chr5:7419131-7419150 MsG0580024632.01.T01:CDS 35.0%
! TTGCTAGGATAAAAGAGCAT+TGG - Chr5:7419146-7419165 MsG0580024632.01.T01:CDS 35.0%
!! AAAGTTGTTTCTCTCCTTCA+AGG - Chr5:7418945-7418964 MsG0580024632.01.T01:CDS 35.0%
ACCATCATCTTCTTCTTCAG+AGG + Chr5:7418769-7418788 None:intergenic 40.0%
ATCTTCTTCTTCAGAGGAAG+AGG + Chr5:7418763-7418782 None:intergenic 40.0%
GAATCTATATCCTTTCGACC+TGG + Chr5:7419052-7419071 None:intergenic 40.0%
! ATTTGCGGAAACATGGATTG+AGG - Chr5:7419098-7419117 MsG0580024632.01.T01:CDS 40.0%
! TCCTCTGAAGAAGAAGATGA+TGG - Chr5:7418765-7418784 MsG0580024632.01.T01:CDS 40.0%
!! CTAGGATAAAAGAGCATTGG+AGG - Chr5:7419149-7419168 MsG0580024632.01.T01:CDS 40.0%
!! GTTTGATTTTCCAGGTCGAA+AGG - Chr5:7419039-7419058 MsG0580024632.01.T01:CDS 40.0%
AGAAGATCGCGATGAAGCTT+CGG - Chr5:7418881-7418900 MsG0580024632.01.T01:intron 45.0%
GCAATCGCTGAGTTGTTCTT+CGG + Chr5:7418808-7418827 None:intergenic 45.0%
! AAACATGGATTGAGGCTTGC+AGG - Chr5:7419106-7419125 MsG0580024632.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr5 gene 7418720 7419174 7418720 ID=MsG0580024632.01;Name=MsG0580024632.01
Chr5 mRNA 7418720 7419174 7418720 ID=MsG0580024632.01.T01;Parent=MsG0580024632.01;Name=MsG0580024632.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|122
Chr5 exon 7418928 7419174 7418928 ID=MsG0580024632.01.T01:exon:1208;Parent=MsG0580024632.01.T01
Chr5 exon 7418720 7418841 7418720 ID=MsG0580024632.01.T01:exon:1207;Parent=MsG0580024632.01.T01
Chr5 CDS 7418928 7419174 7418928 ID=MsG0580024632.01.T01:cds;Parent=MsG0580024632.01.T01
Chr5 CDS 7418720 7418841 7418720 ID=MsG0580024632.01.T01:cds;Parent=MsG0580024632.01.T01
Gene Sequence

>MsG0580024632.01.T01

ATGGATGAGAGGAGAGGTAAAAGAGCAGTGATTGTTCTCTCCTCTTCCTCTGAAGAAGAAGATGATGGTGATTACGACGACGATTCCGAAGAACAACTCAGCGATTGCGATGACTCGTCCCAATCAGATACTAGTGAAATTGAAGATGAGGATGATGATGAAGAAGATCGCGATGAAGCTTCGGAATCAGATAATTGTGATGTTGATGACGAATCTCTCTCCGAAAAAGTTGTTTCTCTCCTTCAAGGTCGAAAGGATATAGATTCGTTGAAATTAAACGAATGCAAAGCATATTTGCGGAAACATGGATTGAGGCTTGCAGGAAATAGAGCAGTTTGTATTGCTAGGATAAAAGAGCATTGGAGGTAA

Protein sequence

>MsG0580024632.01.T01

MDERRGKRAVIVLSSSSEEEDDGDYDDDSEEQLSDCDDSSQSDTSEIEDEDDDEEDRDEASESDNCDVDDESLSEKVVSLLQGRKDIDSLKLNECKAYLRKHGLRLAGNRAVCIARIKEHWR*