Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003454.01.T01 | GAU42721.1 | 87.85 | 107 | 13 | 0 | 6 | 112 | 52 | 158 | 1.61E-61 | 195 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003454.01.T01 | G7IA77 | 97.196 | 107 | 3 | 0 | 6 | 112 | 30 | 136 | 8.61e-61 | 211 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000741.01 | MsG0180003454.01 | 0.808221 | 3.444534e-50 | 1.958369e-47 |
| MsG0180000836.01 | MsG0180003454.01 | 0.815693 | 8.110002e-52 | 5.630805e-49 |
| MsG0180001173.01 | MsG0180003454.01 | 0.814906 | 1.213281e-51 | 8.244078e-49 |
| MsG0180002975.01 | MsG0180003454.01 | 0.804621 | 1.978687e-49 | 1.025014e-46 |
| MsG0180003454.01 | MsG0180005445.01 | 0.815903 | 7.284344e-52 | 5.086263e-49 |
| MsG0180003454.01 | MsG0280006367.01 | 0.801046 | 1.082814e-48 | 5.120632e-46 |
| MsG0180003454.01 | MsG0280008363.01 | 0.807409 | 5.126308e-50 | 2.853217e-47 |
| MsG0180003454.01 | MsG0280008909.01 | 0.809873 | 1.525569e-50 | 9.060935e-48 |
| MsG0180003454.01 | MsG0280009417.01 | 0.801466 | 8.884298e-49 | 4.245797e-46 |
| MsG0180003454.01 | MsG0280009748.01 | 0.800958 | 1.128664e-48 | 5.325490e-46 |
| MsG0180003454.01 | MsG0280010109.01 | 0.823618 | 1.257416e-53 | 1.088219e-50 |
| MsG0180003454.01 | MsG0280011065.01 | 0.803907 | 2.786189e-49 | 1.417178e-46 |
| MsG0180003454.01 | MsG0380013363.01 | 0.803810 | 2.918439e-49 | 1.480654e-46 |
| MsG0180003454.01 | MsG0380014576.01 | 0.800373 | 1.485700e-48 | 6.906658e-46 |
| MsG0180003454.01 | MsG0480019453.01 | 0.805556 | 1.261053e-49 | 6.691612e-47 |
| MsG0180003454.01 | MsG0480022261.01 | 0.818305 | 2.100530e-52 | 1.566574e-49 |
| MsG0180003454.01 | MsG0480023873.01 | 0.814380 | 1.586572e-51 | 1.062965e-48 |
| MsG0180003454.01 | MsG0680034757.01 | 0.812452 | 4.209540e-51 | 2.677704e-48 |
| MsG0180003454.01 | MsG0780039443.01 | 0.803532 | 3.332832e-49 | 1.678950e-46 |
| MsG0180003454.01 | MsG0780041305.01 | 0.827039 | 1.949987e-54 | 1.860636e-51 |
| MsG0180003454.01 | MsG0880042760.01 | 0.801627 | 8.237051e-49 | 3.952315e-46 |
| MsG0180003454.01 | MsG0880043480.01 | 0.812807 | 3.520738e-51 | 2.260197e-48 |
| MsG0180003454.01 | MsG0880044948.01 | 0.817890 | 2.607489e-52 | 1.922568e-49 |
| MsG0180003454.01 | MsG0880046027.01 | 0.811571 | 6.550039e-51 | 4.068739e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003454.01.T01 | MTR_1g062200 | 97.196 | 107 | 3 | 0 | 6 | 112 | 30 | 136 | 2.18e-64 | 211 |
| MsG0180003454.01.T01 | MTR_1g062200 | 97.196 | 107 | 3 | 0 | 6 | 112 | 30 | 136 | 2.59e-64 | 210 |
| MsG0180003454.01.T01 | MTR_1g090807 | 44.954 | 109 | 53 | 3 | 6 | 111 | 54 | 158 | 1.92e-26 | 102 |
| MsG0180003454.01.T01 | MTR_3g073620 | 37.736 | 106 | 66 | 0 | 7 | 112 | 31 | 136 | 3.28e-23 | 93.2 |
| MsG0180003454.01.T01 | MTR_1g100633 | 35.577 | 104 | 67 | 0 | 9 | 112 | 30 | 133 | 8.35e-15 | 69.3 |
| MsG0180003454.01.T01 | MTR_1g100633 | 35.577 | 104 | 67 | 0 | 9 | 112 | 30 | 133 | 8.67e-15 | 69.3 |
| MsG0180003454.01.T01 | MTR_1g100633 | 35.577 | 104 | 67 | 0 | 9 | 112 | 30 | 133 | 8.98e-15 | 69.3 |
| MsG0180003454.01.T01 | MTR_5g010520 | 27.358 | 106 | 77 | 0 | 7 | 112 | 31 | 136 | 2.41e-12 | 62.4 |
| MsG0180003454.01.T01 | MTR_8g097350 | 26.415 | 106 | 78 | 0 | 7 | 112 | 30 | 135 | 2.44e-12 | 62.4 |
| MsG0180003454.01.T01 | MTR_8g097350 | 26.415 | 106 | 78 | 0 | 7 | 112 | 30 | 135 | 3.15e-12 | 62.0 |
| MsG0180003454.01.T01 | MTR_5g010520 | 27.358 | 106 | 77 | 0 | 7 | 112 | 31 | 136 | 3.22e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003454.01.T01 | AT1G22060 | 39.623 | 106 | 64 | 0 | 7 | 112 | 31 | 136 | 3.22e-24 | 96.3 |
| MsG0180003454.01.T01 | AT1G22060 | 39.623 | 106 | 64 | 0 | 7 | 112 | 31 | 136 | 3.22e-24 | 96.3 |
| MsG0180003454.01.T01 | AT1G63300 | 31.068 | 103 | 70 | 1 | 11 | 112 | 35 | 137 | 3.21e-11 | 58.9 |
| MsG0180003454.01.T01 | AT5G52280 | 31.373 | 102 | 70 | 0 | 11 | 112 | 33 | 134 | 7.16e-11 | 58.2 |
Find 25 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGTTGTCTCTGTAGAAAA+TGG | 0.366879 | 1:+62497981 | MsG0180003454.01.T01:CDS |
| CCGCTAAATAAGTGCAATCA+TGG | 0.416997 | 1:+62498319 | MsG0180003454.01.T01:CDS |
| AGCAAAGTCACGGTGCGAAA+TGG | 0.428476 | 1:+62498023 | MsG0180003454.01.T01:CDS |
| ATCATTAAGATCTGGAATCC+TGG | 0.429489 | 1:+62498234 | MsG0180003454.01.T01:CDS |
| CATGATTGCACTTATTTAGC+GGG | 0.430592 | 1:-62498318 | None:intergenic |
| AAACAGTTTGTCCCACCCTT+TGG | 0.455559 | 1:-62497955 | None:intergenic |
| TGCATCTGAACTCGCATAGT+TGG | 0.475884 | 1:-62498278 | None:intergenic |
| AATTGAATTCTAATGTTAGC+AGG | 0.488208 | 1:+62497930 | None:intergenic |
| AATACAATCATCAGTCTCCT+TGG | 0.488486 | 1:-62498105 | None:intergenic |
| TAACAGGGATCATTAAGATC+TGG | 0.494826 | 1:+62498226 | MsG0180003454.01.T01:intron |
| TACTAACTGTGGCCTCTCCC+AGG | 0.496498 | 1:-62498252 | None:intergenic |
| TTATTTAGCGGGATTGAAAG+TGG | 0.524587 | 1:-62498307 | None:intergenic |
| TCTCAAGCTCATTGTTGCAA+TGG | 0.530061 | 1:+62498127 | MsG0180003454.01.T01:CDS |
| CCATGATTGCACTTATTTAG+CGG | 0.533424 | 1:-62498319 | None:intergenic |
| TCATTAAGATCTGGAATCCT+GGG | 0.533611 | 1:+62498235 | MsG0180003454.01.T01:CDS |
| TTCAAGAGATAATTTGTCCA+AGG | 0.536501 | 1:+62498088 | MsG0180003454.01.T01:CDS |
| CGCTAAATAAGTGCAATCAT+GGG | 0.541016 | 1:+62498320 | MsG0180003454.01.T01:CDS |
| CTAATGTTAGCAGGTACCAA+AGG | 0.559324 | 1:+62497939 | None:intergenic |
| TAATGTTAGCAGGTACCAAA+GGG | 0.572824 | 1:+62497940 | None:intergenic |
| TGCAAAGTCAAGCAAAGTCA+CGG | 0.610444 | 1:+62498013 | MsG0180003454.01.T01:CDS |
| AAGATCTGGAATCCTGGGAG+AGG | 0.617399 | 1:+62498240 | MsG0180003454.01.T01:CDS |
| TGTTAGCAGGTACCAAAGGG+TGG | 0.627243 | 1:+62497943 | MsG0180003454.01.T01:CDS |
| GTTAGCAGGTACCAAAGGGT+GGG | 0.655335 | 1:+62497944 | MsG0180003454.01.T01:CDS |
| TGCGAAATGGAAGTTGTCAG+TGG | 0.678162 | 1:+62498036 | MsG0180003454.01.T01:CDS |
| ATAGTTGGTCATACTAACTG+TGG | 0.684689 | 1:-62498263 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATCAGTTTGAAATAACA+GGG | + | Chr1:62498211-62498230 | MsG0180003454.01.T01:intron | 20.0% |
| !! | ATACTTTTGCAGAATCTATA+TGG | + | Chr1:62498063-62498082 | MsG0180003454.01.T01:CDS | 25.0% |
| !! | CTTTATCAGTTTGAAATAAC+AGG | + | Chr1:62498210-62498229 | MsG0180003454.01.T01:intron | 25.0% |
| CTGATAAAGTTAACAGCAAA+AGG | - | Chr1:62498199-62498218 | None:intergenic | 30.0% | |
| ! | TTCAAGAGATAATTTGTCCA+AGG | + | Chr1:62498088-62498107 | MsG0180003454.01.T01:CDS | 30.0% |
| AATACAATCATCAGTCTCCT+TGG | - | Chr1:62498108-62498127 | None:intergenic | 35.0% | |
| ATAGTTGGTCATACTAACTG+TGG | - | Chr1:62498266-62498285 | None:intergenic | 35.0% | |
| CATGATTGCACTTATTTAGC+GGG | - | Chr1:62498321-62498340 | None:intergenic | 35.0% | |
| CCATGATTGCACTTATTTAG+CGG | - | Chr1:62498322-62498341 | None:intergenic | 35.0% | |
| CGCTAAATAAGTGCAATCAT+GGG | + | Chr1:62498320-62498339 | MsG0180003454.01.T01:CDS | 35.0% | |
| TAACAGGGATCATTAAGATC+TGG | + | Chr1:62498226-62498245 | MsG0180003454.01.T01:intron | 35.0% | |
| TCTGTTGTCTCTGTAGAAAA+TGG | + | Chr1:62497981-62498000 | MsG0180003454.01.T01:CDS | 35.0% | |
| TTATTTAGCGGGATTGAAAG+TGG | - | Chr1:62498310-62498329 | None:intergenic | 35.0% | |
| ! | ATCATTAAGATCTGGAATCC+TGG | + | Chr1:62498234-62498253 | MsG0180003454.01.T01:CDS | 35.0% |
| ! | TCATTAAGATCTGGAATCCT+GGG | + | Chr1:62498235-62498254 | MsG0180003454.01.T01:CDS | 35.0% |
| CCGCTAAATAAGTGCAATCA+TGG | + | Chr1:62498319-62498338 | MsG0180003454.01.T01:CDS | 40.0% | |
| TCTCAAGCTCATTGTTGCAA+TGG | + | Chr1:62498127-62498146 | MsG0180003454.01.T01:CDS | 40.0% | |
| TGCAAAGTCAAGCAAAGTCA+CGG | + | Chr1:62498013-62498032 | MsG0180003454.01.T01:CDS | 40.0% | |
| AAACAGTTTGTCCCACCCTT+TGG | - | Chr1:62497958-62497977 | None:intergenic | 45.0% | |
| TGCATCTGAACTCGCATAGT+TGG | - | Chr1:62498281-62498300 | None:intergenic | 45.0% | |
| TGCGAAATGGAAGTTGTCAG+TGG | + | Chr1:62498036-62498055 | MsG0180003454.01.T01:CDS | 45.0% | |
| AGCAAAGTCACGGTGCGAAA+TGG | + | Chr1:62498023-62498042 | MsG0180003454.01.T01:CDS | 50.0% | |
| GTTAGCAGGTACCAAAGGGT+GGG | + | Chr1:62497944-62497963 | MsG0180003454.01.T01:CDS | 50.0% | |
| TGTTAGCAGGTACCAAAGGG+TGG | + | Chr1:62497943-62497962 | MsG0180003454.01.T01:CDS | 50.0% | |
| ! | AAGATCTGGAATCCTGGGAG+AGG | + | Chr1:62498240-62498259 | MsG0180003454.01.T01:CDS | 50.0% |
| TACTAACTGTGGCCTCTCCC+AGG | - | Chr1:62498255-62498274 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 62497942 | 62498363 | 62497942 | ID=MsG0180003454.01;Name=MsG0180003454.01 |
| Chr1 | mRNA | 62497942 | 62498363 | 62497942 | ID=MsG0180003454.01.T01;Parent=MsG0180003454.01;Name=MsG0180003454.01.T01;_AED=0.49;_eAED=0.50;_QI=0|1|0|1|1|1|2|0|112 |
| Chr1 | exon | 62497942 | 62498148 | 62497942 | ID=MsG0180003454.01.T01:exon:4536;Parent=MsG0180003454.01.T01 |
| Chr1 | exon | 62498232 | 62498363 | 62498232 | ID=MsG0180003454.01.T01:exon:4537;Parent=MsG0180003454.01.T01 |
| Chr1 | CDS | 62497942 | 62498148 | 62497942 | ID=MsG0180003454.01.T01:cds;Parent=MsG0180003454.01.T01 |
| Chr1 | CDS | 62498232 | 62498363 | 62498232 | ID=MsG0180003454.01.T01:cds;Parent=MsG0180003454.01.T01 |
| Gene Sequence |
| Protein sequence |
