AlfalfaGEDB | MsG0180004434.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004434.01

KEGG: K03320
GO: GO:0008519 GO:0015696 GO:0016020
PFAM: PF00909

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004434.01.T01	XP_003590923.2	91.25	80	7	0	1	80	440	519	8.48E-44	158

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004434.01.T01	O04161	58.667	75	29	1	1	75	442	514	8.56E-23	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004434.01.T01	G7I667	91.250	80	7	0	1	80	440	519	4.05e-44	158

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048698.01	MsG0180004434.01	0.833631	4.773442e-56	5.528797e-53
MsG0180004434.01	MsG0180004435.01	0.899381	2.097366e-77	2.587415e-73
MsG0180004434.01	MsG0280007042.01	0.807657	4.541567e-50	2.543939e-47
MsG0180004434.01	MsG0280010145.01	0.825114	5.592030e-54	5.050383e-51
MsG0180004434.01	MsG0380013916.01	0.825503	4.525159e-54	4.132094e-51
MsG0180004434.01	MsG0380013917.01	0.811455	6.941602e-51	4.298896e-48
MsG0180004434.01	MsG0380013918.01	0.828716	7.700328e-55	7.715599e-52
MsG0180004434.01	MsG0380013920.01	0.812860	3.428037e-51	2.203799e-48
MsG0180004434.01	MsG0380013929.01	0.818206	2.212408e-52	1.645471e-49
MsG0180004434.01	MsG0380013931.01	0.848551	5.704598e-60	1.050694e-56
MsG0180004434.01	MsG0380013932.01	0.841081	5.897019e-58	8.568012e-55
MsG0180004434.01	MsG0380013942.01	0.830209	3.340878e-55	3.496773e-52
MsG0180004434.01	MsG0380013948.01	0.837833	4.116092e-57	5.412547e-54
MsG0180004434.01	MsG0380013952.01	0.809219	2.108321e-50	1.230695e-47
MsG0180004434.01	MsG0380013966.01	0.800559	1.361855e-48	6.360553e-46
MsG0180004434.01	MsG0380013969.01	0.809270	2.055761e-50	1.201621e-47
MsG0180004434.01	MsG0380013972.01	0.822717	2.040579e-53	1.721605e-50
MsG0180004434.01	MsG0380013981.01	0.853853	1.816054e-61	3.978601e-58
MsG0180004434.01	MsG0380013983.01	0.806474	8.081846e-50	4.390751e-47
MsG0180004434.01	MsG0380013995.01	0.826108	3.250542e-54	3.019886e-51
MsG0180004434.01	MsG0380014008.01	0.806542	7.820136e-50	4.255955e-47
MsG0180004434.01	MsG0380014023.01	0.810781	9.720169e-51	5.913191e-48
MsG0180004434.01	MsG0380014035.01	0.808657	2.780955e-50	1.599117e-47
MsG0180004434.01	MsG0380014036.01	0.844721	6.343415e-59	1.033300e-55
MsG0180004434.01	MsG0380014051.01	0.821152	4.699678e-53	3.793213e-50
MsG0180004434.01	MsG0380017007.01	0.807768	4.301887e-50	2.416677e-47
MsG0180004434.01	MsG0780040316.01	0.839732	1.329148e-57	1.852012e-54
MsG0180004434.01	MsG0780040317.01	0.803895	2.801816e-49	1.424707e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004434.01.T01	MTR_1g079760	91.250	80	7	0	1	80	440	519	1.03e-47	158
MsG0180004434.01.T01	MTR_1g045550	55.224	67	26	1	1	67	438	500	3.20e-17	74.7
MsG0180004434.01.T01	MTR_3g463730	55.556	45	20	0	1	45	404	448	9.21e-12	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004434.01.T01	AT1G64780	63.235	68	23	2	1	67	445	511	2.22e-22	89.4
MsG0180004434.01.T01	AT4G13510	54.667	75	28	4	1	75	433	501	2.63e-16	72.0
MsG0180004434.01.T01	AT3G24300	49.254	67	25	2	1	67	437	494	9.79e-13	62.0
MsG0180004434.01.T01	AT3G24290	60.465	43	17	0	1	43	436	478	2.08e-11	58.2

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACGAGTCTATTAGGAAATT+AGG	0.214176	1:-77895177	MsG0180004434.01.T01:CDS
AGGAAGATGACGAGTCTATT+AGG	0.343254	1:-77895185	MsG0180004434.01.T01:CDS
ACGAGTCTATTAGGAAATTA+GGG	0.347347	1:-77895176	MsG0180004434.01.T01:CDS
CACACTGAAGGTGCACTGAT+TGG	0.453333	1:+77895084	None:intergenic
TAGGAAATTAGGGTTTATGA+TGG	0.464007	1:-77895166	MsG0180004434.01.T01:CDS
TCGAGGGATGATGAGACTGC+AGG	0.509046	1:-77895255	MsG0180004434.01.T01:CDS
TGGCACCGACGTATCGTGTT+CGG	0.511058	1:+77895104	None:intergenic
AATTAGGGTTTATGATGGGT+AGG	0.517824	1:-77895161	MsG0180004434.01.T01:CDS
CGTATCGTGTTCGGTGGCAG+AGG	0.523564	1:+77895113	None:intergenic
GAGGGAGACACACTCATGGC+CGG	0.540366	1:+77895132	None:intergenic
TATGATGGGTAGGATTGAGC+CGG	0.553940	1:-77895151	MsG0180004434.01.T01:CDS
GGATGATGAGACTGCAGGGA+TGG	0.557751	1:-77895250	MsG0180004434.01.T01:CDS
TGAAATTGTTGAGGATATCG+AGG	0.567161	1:-77895272	MsG0180004434.01.T01:CDS
TTATTCAGCCCATAAAATAA+CGG	0.567889	1:+77895297	None:intergenic
CGAGGGATGATGAGACTGCA+GGG	0.589259	1:-77895254	MsG0180004434.01.T01:CDS
TGAATAAGATGAAATTGTTG+AGG	0.592343	1:-77895281	MsG0180004434.01.T01:CDS
AGGAAATTAGGGTTTATGAT+GGG	0.592652	1:-77895165	MsG0180004434.01.T01:CDS
GGCAGAGGGAGACACACTCA+TGG	0.604115	1:+77895128	None:intergenic
TGGACTTGACTAGACATGGT+GGG	0.625359	1:-77895230	MsG0180004434.01.T01:CDS
GAAATTGTTGAGGATATCGA+GGG	0.633706	1:-77895271	MsG0180004434.01.T01:CDS
CACCGACGTATCGTGTTCGG+TGG	0.638372	1:+77895107	None:intergenic
GTATCGTGTTCGGTGGCAGA+GGG	0.638569	1:+77895114	None:intergenic
TGCTTATGCGTATCATGACG+AGG	0.643185	1:-77895205	MsG0180004434.01.T01:CDS
AAGTGATGATATCACACTGA+AGG	0.666643	1:+77895072	None:intergenic
GGGATGGACTTGACTAGACA+TGG	0.695287	1:-77895234	MsG0180004434.01.T01:CDS
ATGGACTTGACTAGACATGG+TGG	0.698702	1:-77895231	MsG0180004434.01.T01:CDS
TGCCACCGAACACGATACGT+CGG	0.704524	1:-77895109	MsG0180004434.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGAATAAGATGAAATTGTTG+AGG	-	Chr1:77895105-77895124	MsG0180004434.01.T01:CDS	25.0%
!	TTATTCAGCCCATAAAATAA+CGG	+	Chr1:77895092-77895111	None:intergenic	25.0%
	ACGAGTCTATTAGGAAATTA+GGG	-	Chr1:77895210-77895229	MsG0180004434.01.T01:CDS	30.0%
	AGGAAATTAGGGTTTATGAT+GGG	-	Chr1:77895221-77895240	MsG0180004434.01.T01:CDS	30.0%
	TAGGAAATTAGGGTTTATGA+TGG	-	Chr1:77895220-77895239	MsG0180004434.01.T01:CDS	30.0%
	GAAATTGTTGAGGATATCGA+GGG	-	Chr1:77895115-77895134	MsG0180004434.01.T01:CDS	35.0%
	GACGAGTCTATTAGGAAATT+AGG	-	Chr1:77895209-77895228	MsG0180004434.01.T01:CDS	35.0%
	TGAAATTGTTGAGGATATCG+AGG	-	Chr1:77895114-77895133	MsG0180004434.01.T01:CDS	35.0%
!!	AATTAGGGTTTATGATGGGT+AGG	-	Chr1:77895225-77895244	MsG0180004434.01.T01:CDS	35.0%
	AGGAAGATGACGAGTCTATT+AGG	-	Chr1:77895201-77895220	MsG0180004434.01.T01:CDS	40.0%
	ATGGACTTGACTAGACATGG+TGG	-	Chr1:77895155-77895174	MsG0180004434.01.T01:CDS	45.0%
	TGCTTATGCGTATCATGACG+AGG	-	Chr1:77895181-77895200	MsG0180004434.01.T01:CDS	45.0%
	TGGACTTGACTAGACATGGT+GGG	-	Chr1:77895156-77895175	MsG0180004434.01.T01:CDS	45.0%
!!	TATGATGGGTAGGATTGAGC+CGG	-	Chr1:77895235-77895254	MsG0180004434.01.T01:CDS	45.0%
!	CACACTGAAGGTGCACTGAT+TGG	+	Chr1:77895305-77895324	None:intergenic	50.0%
!	GGGATGGACTTGACTAGACA+TGG	-	Chr1:77895152-77895171	MsG0180004434.01.T01:CDS	50.0%
	CGAGGGATGATGAGACTGCA+GGG	-	Chr1:77895132-77895151	MsG0180004434.01.T01:CDS	55.0%
	GGATGATGAGACTGCAGGGA+TGG	-	Chr1:77895136-77895155	MsG0180004434.01.T01:CDS	55.0%
	TCGAGGGATGATGAGACTGC+AGG	-	Chr1:77895131-77895150	MsG0180004434.01.T01:CDS	55.0%
	TGCCACCGAACACGATACGT+CGG	-	Chr1:77895277-77895296	MsG0180004434.01.T01:CDS	55.0%
!	GTATCGTGTTCGGTGGCAGA+GGG	+	Chr1:77895275-77895294	None:intergenic	55.0%
!!	TGGCACCGACGTATCGTGTT+CGG	+	Chr1:77895285-77895304	None:intergenic	55.0%
	CACCGACGTATCGTGTTCGG+TGG	+	Chr1:77895282-77895301	None:intergenic	60.0%
	GAGGGAGACACACTCATGGC+CGG	+	Chr1:77895257-77895276	None:intergenic	60.0%
	GGCAGAGGGAGACACACTCA+TGG	+	Chr1:77895261-77895280	None:intergenic	60.0%
!	CGTATCGTGTTCGGTGGCAG+AGG	+	Chr1:77895276-77895295	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	77895083	77895325	77895083	ID=MsG0180004434.01;Name=MsG0180004434.01
Chr1	mRNA	77895083	77895325	77895083	ID=MsG0180004434.01.T01;Parent=MsG0180004434.01;Name=MsG0180004434.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|80
Chr1	exon	77895083	77895325	77895083	ID=MsG0180004434.01.T01:exon:15042;Parent=MsG0180004434.01.T01
Chr1	CDS	77895083	77895325	77895083	ID=MsG0180004434.01.T01:cds;Parent=MsG0180004434.01.T01

Gene Sequence	>MsG0180004434.01.T01 ATGGTGCCGTTATTTTATGGGCTGAATAAGATGAAATTGTTGAGGATATCGAGGGATGATGAGACTGCAGGGATGGACTTGACTAGACATGGTGGGTTTGCTTATGCGTATCATGACGAGGAAGATGACGAGTCTATTAGGAAATTAGGGTTTATGATGGGTAGGATTGAGCCGGCCATGAGTGTGTCTCCCTCTGCCACCGAACACGATACGTCGGTGCCAATCAGTGCACCTTCAGTGTGA
Protein sequence	>MsG0180004434.01.T01 MVPLFYGLNKMKLLRISRDDETAGMDLTRHGGFAYAYHDEEDDESIRKLGFMMGRIEPAMSVSPSATEHDTSVPISAPSV*