AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013952.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013952.01.T01 MTR_7g021700 99.459 185 1 0 1 185 106 290 9.61e-122 357
MsG0380013952.01.T01 MTR_6g042200 98.315 178 3 0 8 185 383 560 1.97e-113 338
MsG0380013952.01.T01 MTR_4g006100 98.876 178 2 0 8 185 383 560 8.89e-113 342
MsG0380013952.01.T01 MTR_0002s0150 79.144 187 38 1 2 188 411 596 3.41e-94 286
MsG0380013952.01.T01 MTR_3g113820 80.000 185 8 2 1 185 1 156 1.21e-84 247
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013952.01.T01 ATCG01100 83.240 179 30 0 1 179 1 179 3.93e-98 288
MsG0380013952.01.T01 ATMG01275-2 42.453 106 61 0 47 152 23 128 6.69e-23 89.4
MsG0380013952.01.T01 AT2G07785 43.878 98 55 0 56 153 2 99 3.01e-21 84.0

Find 33 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAATTGATATTACTGATA+TGG 0.214930 3:-48499570 None:intergenic
GGACTTCTTATGTCAGGATA+TGG 0.218335 3:+48499692 MsG0380013952.01.T01:CDS
CCCGAATACGCTGGTCCTTT+TGG 0.250915 3:+48499452 MsG0380013952.01.T01:CDS
GATAAGACACATAGAGTTAA+TGG 0.275977 3:-48499782 None:intergenic
TCCAAAAGGACCAGCGTATT+CGG 0.297771 3:-48499453 None:intergenic
GAATCTTTCAAAGAGGTCTA+TGG 0.337868 3:+48499314 MsG0380013952.01.T01:CDS
AGCTCGTAGACCACCCAAAA+AGG 0.338031 3:-48499732 None:intergenic
AGTTATTCAGTAATTCCTTT+TGG 0.358580 3:+48499596 MsG0380013952.01.T01:CDS
AAGAGAATCTTATTCCATCT+AGG 0.379925 3:+48499516 MsG0380013952.01.T01:CDS
AAGAGGTCTATGGAATTCTA+TGG 0.381274 3:+48499324 MsG0380013952.01.T01:CDS
AAACGAATATCTCCCCTAGA+TGG 0.392761 3:-48499530 None:intergenic
CCAAAAGGACCAGCGTATTC+GGG 0.397012 3:-48499452 None:intergenic
CCCAATACAAGAATCAAAAT+AGG 0.405056 3:-48499356 None:intergenic
CCCATTGGACTTCTTATGTC+AGG 0.412953 3:+48499686 MsG0380013952.01.T01:CDS
GACTTCTTATGTCAGGATAT+GGG 0.454242 3:+48499693 MsG0380013952.01.T01:CDS
AGAGAATCTTATTCCATCTA+GGG 0.476104 3:+48499517 MsG0380013952.01.T01:CDS
GATATTCGTTTATTCAGTAT+CGG 0.488534 3:+48499542 MsG0380013952.01.T01:CDS
ATTTCGAGTATTGCTCCCAT+TGG 0.490306 3:+48499671 MsG0380013952.01.T01:CDS
TAAGTGTACTAGCAATTGTA+TGG 0.498343 3:+48499387 MsG0380013952.01.T01:CDS
AAAACAAAGTTATAGCCAAA+AGG 0.501030 3:-48499611 None:intergenic
TTAGAAAGAGAAATATCTGC+AGG 0.509446 3:+48499410 MsG0380013952.01.T01:CDS
GCAGGGATACAACAGCGTAT+TGG 0.516727 3:+48499428 MsG0380013952.01.T01:CDS
CTCTTTGAAAGATTCCAATC+CGG 0.519904 3:-48499306 None:intergenic
TCCTGACATAAGAAGTCCAA+TGG 0.542469 3:-48499687 None:intergenic
CGGATTGGAATCTTTCAAAG+AGG 0.552668 3:+48499307 MsG0380013952.01.T01:CDS
CCTGACATAAGAAGTCCAAT+GGG 0.565703 3:-48499686 None:intergenic
AGAGCTTGAAGAATTCCAAA+AGG 0.569368 3:-48499467 None:intergenic
ATTGATATTACTGATATGGA+TGG 0.591695 3:-48499566 None:intergenic
TGAAATGATAATTGATACAA+CGG 0.611394 3:+48499259 None:intergenic
GAGAATCTTATTCCATCTAG+GGG 0.627938 3:+48499518 MsG0380013952.01.T01:CDS
ATTCTTCAAGCTCTAGCAGA+CGG 0.642740 3:+48499476 MsG0380013952.01.T01:CDS
TAGAAAGAGAAATATCTGCA+GGG 0.656230 3:+48499411 MsG0380013952.01.T01:CDS
CGTATTGGACCCGAATACGC+TGG 0.662476 3:+48499443 MsG0380013952.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATAAATATTCCTTTT+TGG + Chr3:48499718-48499737 MsG0380013952.01.T01:CDS 10.0%
!!! AATAATAAATATTCCTTTTT+GGG + Chr3:48499719-48499738 MsG0380013952.01.T01:CDS 10.0%
!! TAGAATTGATATTACTGATA+TGG - Chr3:48499573-48499592 None:intergenic 20.0%
!!! CAAGATATAAATTCTTTTTC+CGG + Chr3:48499287-48499306 MsG0380013952.01.T01:CDS 20.0%
!!! GTTTTATCTGATTTCAATAT+CGG + Chr3:48499629-48499648 MsG0380013952.01.T01:CDS 20.0%
! AAAACAAAGTTATAGCCAAA+AGG - Chr3:48499614-48499633 None:intergenic 25.0%
! ATTGATATTACTGATATGGA+TGG - Chr3:48499569-48499588 None:intergenic 25.0%
! CGGAAAAAGAATTTATATCT+TGG - Chr3:48499289-48499308 None:intergenic 25.0%
! GATATTCGTTTATTCAGTAT+CGG + Chr3:48499542-48499561 MsG0380013952.01.T01:CDS 25.0%
!! AGTTATTCAGTAATTCCTTT+TGG + Chr3:48499596-48499615 MsG0380013952.01.T01:CDS 25.0%
!! TATAAATTCTTTTTCCGGAT+TGG + Chr3:48499292-48499311 MsG0380013952.01.T01:CDS 25.0%
!!! AATAAATATTCCTTTTTGGG+TGG + Chr3:48499722-48499741 MsG0380013952.01.T01:CDS 25.0%
!!! ACCTATTTTGATTCTTGTAT+TGG + Chr3:48499355-48499374 MsG0380013952.01.T01:CDS 25.0%
!!! CCTATTTTGATTCTTGTATT+GGG + Chr3:48499356-48499375 MsG0380013952.01.T01:CDS 25.0%
!!! TCAATATCGGTGTTTTTTTA+TGG + Chr3:48499642-48499661 MsG0380013952.01.T01:CDS 25.0%
AAGAGAATCTTATTCCATCT+AGG + Chr3:48499516-48499535 MsG0380013952.01.T01:CDS 30.0%
AGAGAATCTTATTCCATCTA+GGG + Chr3:48499517-48499536 MsG0380013952.01.T01:CDS 30.0%
CCCAATACAAGAATCAAAAT+AGG - Chr3:48499359-48499378 None:intergenic 30.0%
GATAAGACACATAGAGTTAA+TGG - Chr3:48499785-48499804 None:intergenic 30.0%
TAAGTGTACTAGCAATTGTA+TGG + Chr3:48499387-48499406 MsG0380013952.01.T01:CDS 30.0%
TAGAAAGAGAAATATCTGCA+GGG + Chr3:48499411-48499430 MsG0380013952.01.T01:CDS 30.0%
TTAGAAAGAGAAATATCTGC+AGG + Chr3:48499410-48499429 MsG0380013952.01.T01:CDS 30.0%
AAGAGGTCTATGGAATTCTA+TGG + Chr3:48499324-48499343 MsG0380013952.01.T01:CDS 35.0%
AGAGCTTGAAGAATTCCAAA+AGG - Chr3:48499470-48499489 None:intergenic 35.0%
GAATCTTTCAAAGAGGTCTA+TGG + Chr3:48499314-48499333 MsG0380013952.01.T01:CDS 35.0%
GACTTCTTATGTCAGGATAT+GGG + Chr3:48499693-48499712 MsG0380013952.01.T01:CDS 35.0%
GAGAATCTTATTCCATCTAG+GGG + Chr3:48499518-48499537 MsG0380013952.01.T01:CDS 35.0%
! CTCTTTGAAAGATTCCAATC+CGG - Chr3:48499309-48499328 None:intergenic 35.0%
AAACGAATATCTCCCCTAGA+TGG - Chr3:48499533-48499552 None:intergenic 40.0%
CCTGACATAAGAAGTCCAAT+GGG - Chr3:48499689-48499708 None:intergenic 40.0%
GGACTTCTTATGTCAGGATA+TGG + Chr3:48499692-48499711 MsG0380013952.01.T01:CDS 40.0%
TCCTGACATAAGAAGTCCAA+TGG - Chr3:48499690-48499709 None:intergenic 40.0%
! ATTCTTCAAGCTCTAGCAGA+CGG + Chr3:48499476-48499495 MsG0380013952.01.T01:CDS 40.0%
! ATTTCGAGTATTGCTCCCAT+TGG + Chr3:48499671-48499690 MsG0380013952.01.T01:CDS 40.0%
! CGGATTGGAATCTTTCAAAG+AGG + Chr3:48499307-48499326 MsG0380013952.01.T01:CDS 40.0%
CCCATTGGACTTCTTATGTC+AGG + Chr3:48499686-48499705 MsG0380013952.01.T01:CDS 45.0%
TCCAAAAGGACCAGCGTATT+CGG - Chr3:48499456-48499475 None:intergenic 45.0%
AGCTCGTAGACCACCCAAAA+AGG - Chr3:48499735-48499754 None:intergenic 50.0%
CCAAAAGGACCAGCGTATTC+GGG - Chr3:48499455-48499474 None:intergenic 50.0%
GCAGGGATACAACAGCGTAT+TGG + Chr3:48499428-48499447 MsG0380013952.01.T01:CDS 50.0%
CGTATTGGACCCGAATACGC+TGG + Chr3:48499443-48499462 MsG0380013952.01.T01:CDS 55.0%
! CCCGAATACGCTGGTCCTTT+TGG + Chr3:48499452-48499471 MsG0380013952.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48499263 48499829 48499263 ID=MsG0380013952.01;Name=MsG0380013952.01
Chr3 mRNA 48499263 48499829 48499263 ID=MsG0380013952.01.T01;Parent=MsG0380013952.01;Name=MsG0380013952.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|188
Chr3 exon 48499263 48499829 48499263 ID=MsG0380013952.01.T01:exon:30488;Parent=MsG0380013952.01.T01
Chr3 CDS 48499263 48499829 48499263 ID=MsG0380013952.01.T01:cds;Parent=MsG0380013952.01.T01
Gene Sequence

>MsG0380013952.01.T01

ATGATAATTGATACAACGGAAGTCCAAGATATAAATTCTTTTTCCGGATTGGAATCTTTCAAAGAGGTCTATGGAATTCTATGGATACTTGTACCTATTTTGATTCTTGTATTGGGAATCACAATAAGTGTACTAGCAATTGTATGGTTAGAAAGAGAAATATCTGCAGGGATACAACAGCGTATTGGACCCGAATACGCTGGTCCTTTTGGAATTCTTCAAGCTCTAGCAGACGGAACAAAACTACTATTCAAAGAGAATCTTATTCCATCTAGGGGAGATATTCGTTTATTCAGTATCGGACCATCCATATCAGTAATATCAATTCTAATAAGTTATTCAGTAATTCCTTTTGGCTATAACTTTGTTTTATCTGATTTCAATATCGGTGTTTTTTTATGGATTGCTATTTCGAGTATTGCTCCCATTGGACTTCTTATGTCAGGATATGGGTCAAATAATAAATATTCCTTTTTGGGTGGTCTACGAGCTGCTGCTCAATCGATTAGTTATGAAATACCATTAACTCTATGTGTCTTATCAATATCTCTACGTGCGATTCGTTGA

Protein sequence

>MsG0380013952.01.T01

MIIDTTEVQDINSFSGLESFKEVYGILWILVPILILVLGITISVLAIVWLEREISAGIQQRIGPEYAGPFGILQALADGTKLLFKENLIPSRGDIRLFSIGPSISVISILISYSVIPFGYNFVLSDFNIGVFLWIAISSIAPIGLLMSGYGSNNKYSFLGGLRAAAQSISYEIPLTLCVLSISLRAIR*