AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013972.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013972.01.T01 MTR_6g042200 96.639 119 4 0 1 119 268 386 6.69e-75 235
MsG0380013972.01.T01 MTR_4g006100 97.479 119 3 0 1 119 268 386 8.77e-75 238
MsG0380013972.01.T01 MTR_0002s0150 78.992 119 25 0 1 119 299 417 2.16e-62 200
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013972.01.T01 ATCG01110 90.476 126 12 0 1 126 268 393 5.17e-77 233
MsG0380013972.01.T01 ATMG00510 37.594 133 70 3 1 126 268 394 4.05e-18 79.0

Find 21 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAGTTAAAAGAATGAAAT+TGG 0.240964 3:+48543755 MsG0380013972.01.T01:CDS
TAAAATTATTCAACAGGCTT+TGG 0.308807 3:+48543470 MsG0380013972.01.T01:CDS
TCTCACATAAAGTTCTTGTT+TGG 0.322955 3:-48543619 None:intergenic
ATCCATTAAAATTATTCAAC+AGG 0.326815 3:+48543464 MsG0380013972.01.T01:CDS
TATTCAAAATCATTCCATTC+TGG 0.326932 3:-48543555 None:intergenic
AGGTAGCATAGATATCATTA+TGG 0.351209 3:+48543797 MsG0380013972.01.T01:CDS
CAGGCTTTGGAAGGAATTCC+AGG 0.354309 3:+48543483 MsG0380013972.01.T01:CDS
CTCACATAAAGTTCTTGTTT+GGG 0.400339 3:-48543618 None:intergenic
GGTAGCATAGATATCATTAT+GGG 0.462662 3:+48543798 MsG0380013972.01.T01:CDS
TCGCTCCTTTGATAGAGAAA+AGG 0.480513 3:+48543530 MsG0380013972.01.T01:CDS
GCTGATATTATGACAATACT+AGG 0.522631 3:+48543777 MsG0380013972.01.T01:CDS
ATAGAGAAAAGGAGCCAGAA+TGG 0.528576 3:+48543541 MsG0380013972.01.T01:CDS
AGATGGAAAATTCGTCCACC+TGG 0.529045 3:+48543705 MsG0380013972.01.T01:CDS
ATTATTCAACAGGCTTTGGA+AGG 0.539344 3:+48543474 MsG0380013972.01.T01:CDS
TGTTTGGGCAATTCAAAAGT+CGG 0.557531 3:-48543603 None:intergenic
TTTCTAATAGGAGATCAGAA+TGG 0.560048 3:+48543672 MsG0380013972.01.T01:CDS
ATTTGCAAATTGATAAAACC+AGG 0.570420 3:-48543723 None:intergenic
AGAACTTTATGTGAGAGTTG+AGG 0.641734 3:+48543626 MsG0380013972.01.T01:CDS
CTTTGGAAGGAATTCCAGGT+GGG 0.666113 3:+48543487 MsG0380013972.01.T01:CDS
TGCAAATTGATAAAACCAGG+TGG 0.677686 3:-48543720 None:intergenic
GCTTTGGAAGGAATTCCAGG+TGG 0.694925 3:+48543486 MsG0380013972.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAGTTAAAAGAATGAAAT+TGG + Chr3:48543755-48543774 MsG0380013972.01.T01:CDS 15.0%
!! ATCCATTAAAATTATTCAAC+AGG + Chr3:48543464-48543483 MsG0380013972.01.T01:CDS 20.0%
!!! CGAATTTCTAAATTTTCATA+TGG - Chr3:48543513-48543532 None:intergenic 20.0%
!!! GAATTAGGAATTTTTCTAAT+AGG + Chr3:48543660-48543679 MsG0380013972.01.T01:CDS 20.0%
!!! TTCATTCTTTTAACTAATTG+AGG - Chr3:48543753-48543772 None:intergenic 20.0%
! ATTTGCAAATTGATAAAACC+AGG - Chr3:48543726-48543745 None:intergenic 25.0%
! TAAAATTATTCAACAGGCTT+TGG + Chr3:48543470-48543489 MsG0380013972.01.T01:CDS 25.0%
! TATTCAAAATCATTCCATTC+TGG - Chr3:48543558-48543577 None:intergenic 25.0%
!! AGCCTGTTGAATAATTTTAA+TGG - Chr3:48543469-48543488 None:intergenic 25.0%
!!! GATTTTGAATATCGATTCAT+TGG + Chr3:48543567-48543586 MsG0380013972.01.T01:CDS 25.0%
AGGTAGCATAGATATCATTA+TGG + Chr3:48543797-48543816 MsG0380013972.01.T01:CDS 30.0%
GCTGATATTATGACAATACT+AGG + Chr3:48543777-48543796 MsG0380013972.01.T01:CDS 30.0%
GGTAGCATAGATATCATTAT+GGG + Chr3:48543798-48543817 MsG0380013972.01.T01:CDS 30.0%
TCTCACATAAAGTTCTTGTT+TGG - Chr3:48543622-48543641 None:intergenic 30.0%
TTTCTAATAGGAGATCAGAA+TGG + Chr3:48543672-48543691 MsG0380013972.01.T01:CDS 30.0%
! CTCACATAAAGTTCTTGTTT+GGG - Chr3:48543621-48543640 None:intergenic 30.0%
AAAAATTCCTAATTCCCCCT+TGG - Chr3:48543655-48543674 None:intergenic 35.0%
AAAATTCCTAATTCCCCCTT+GGG - Chr3:48543654-48543673 None:intergenic 35.0%
AGAACTTTATGTGAGAGTTG+AGG + Chr3:48543626-48543645 MsG0380013972.01.T01:CDS 35.0%
TGCAAATTGATAAAACCAGG+TGG - Chr3:48543723-48543742 None:intergenic 35.0%
TGTTTGGGCAATTCAAAAGT+CGG - Chr3:48543606-48543625 None:intergenic 35.0%
!! ATTATTCAACAGGCTTTGGA+AGG + Chr3:48543474-48543493 MsG0380013972.01.T01:CDS 35.0%
AAATTCCTAATTCCCCCTTG+GGG - Chr3:48543653-48543672 None:intergenic 40.0%
ATAGAGAAAAGGAGCCAGAA+TGG + Chr3:48543541-48543560 MsG0380013972.01.T01:CDS 40.0%
TCGCTCCTTTGATAGAGAAA+AGG + Chr3:48543530-48543549 MsG0380013972.01.T01:CDS 40.0%
! AAATTTTCATATGGCCCACC+TGG - Chr3:48543504-48543523 None:intergenic 40.0%
! AGAATGGTTTTCCTTGGAGA+TGG + Chr3:48543688-48543707 MsG0380013972.01.T01:CDS 40.0%
! GAGATCAGAATGGTTTTCCT+TGG + Chr3:48543682-48543701 MsG0380013972.01.T01:CDS 40.0%
! TGGCTCCTTTTCTCTATCAA+AGG - Chr3:48543538-48543557 None:intergenic 40.0%
AATTCCTAATTCCCCCTTGG+GGG - Chr3:48543652-48543671 None:intergenic 45.0%
AGATGGAAAATTCGTCCACC+TGG + Chr3:48543705-48543724 MsG0380013972.01.T01:CDS 45.0%
! CTTTGGAAGGAATTCCAGGT+GGG + Chr3:48543487-48543506 MsG0380013972.01.T01:CDS 45.0%
! GGACGAATTTTCCATCTCCA+AGG - Chr3:48543702-48543721 None:intergenic 45.0%
!! CAGGCTTTGGAAGGAATTCC+AGG + Chr3:48543483-48543502 MsG0380013972.01.T01:CDS 50.0%
!! GCTTTGGAAGGAATTCCAGG+TGG + Chr3:48543486-48543505 MsG0380013972.01.T01:CDS 50.0%
GTGAGAGTTGAGGCCCCCAA+GGG + Chr3:48543636-48543655 MsG0380013972.01.T01:CDS 60.0%
TGAGAGTTGAGGCCCCCAAG+GGG + Chr3:48543637-48543656 MsG0380013972.01.T01:CDS 60.0%
TGTGAGAGTTGAGGCCCCCA+AGG + Chr3:48543635-48543654 MsG0380013972.01.T01:CDS 60.0%
GAGAGTTGAGGCCCCCAAGG+GGG + Chr3:48543638-48543657 MsG0380013972.01.T01:CDS 65.0%
GAGGCCCCCAAGGGGGAATT+AGG + Chr3:48543645-48543664 MsG0380013972.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48543456 48543836 48543456 ID=MsG0380013972.01;Name=MsG0380013972.01
Chr3 mRNA 48543456 48543836 48543456 ID=MsG0380013972.01.T01;Parent=MsG0380013972.01;Name=MsG0380013972.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr3 exon 48543456 48543836 48543456 ID=MsG0380013972.01.T01:exon:8493;Parent=MsG0380013972.01.T01
Chr3 CDS 48543456 48543836 48543456 ID=MsG0380013972.01.T01:cds;Parent=MsG0380013972.01.T01
Gene Sequence

>MsG0380013972.01.T01

ATGATGGAATCCATTAAAATTATTCAACAGGCTTTGGAAGGAATTCCAGGTGGGCCATATGAAAATTTAGAAATTCGCTCCTTTGATAGAGAAAAGGAGCCAGAATGGAATGATTTTGAATATCGATTCATTGGTAAAAAATCGTCTCCGACTTTTGAATTGCCCAAACAAGAACTTTATGTGAGAGTTGAGGCCCCCAAGGGGGAATTAGGAATTTTTCTAATAGGAGATCAGAATGGTTTTCCTTGGAGATGGAAAATTCGTCCACCTGGTTTTATCAATTTGCAAATTCTTCCTCAATTAGTTAAAAGAATGAAATTGGCTGATATTATGACAATACTAGGTAGCATAGATATCATTATGGGAGAAGTTGATCGTTGA

Protein sequence

>MsG0380013972.01.T01

MMESIKIIQQALEGIPGGPYENLEIRSFDREKEPEWNDFEYRFIGKKSSPTFELPKQELYVRVEAPKGELGIFLIGDQNGFPWRWKIRPPGFINLQILPQLVKRMKLADIMTILGSIDIIMGEVDR*