Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004847.01.T01 | XP_024642160.1 | 64.865 | 111 | 39 | 0 | 3 | 113 | 140 | 250 | 3.27E-44 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004847.01.T01 | A2Q571 | 82.716 | 81 | 14 | 0 | 1 | 81 | 1 | 81 | 2.56e-41 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002518.01 | MsG0180004847.01 | 0.820797 | 5.674087e-53 | 4.534512e-50 |
| MsG0180004847.01 | MsG0280006325.01 | 0.812611 | 3.885913e-51 | 2.482022e-48 |
| MsG0180004847.01 | MsG0280009306.01 | 0.829973 | 3.814194e-55 | 3.964944e-52 |
| MsG0180004847.01 | MsG0380014977.01 | 0.814335 | 1.623484e-51 | 1.086322e-48 |
| MsG0180004847.01 | MsG0380016274.01 | 0.809903 | 1.503295e-50 | 8.935650e-48 |
| MsG0180004847.01 | MsG0380017312.01 | 0.810386 | 1.182708e-50 | 7.119043e-48 |
| MsG0180004847.01 | MsG0480018664.01 | 0.814430 | 1.546664e-51 | 1.037604e-48 |
| MsG0180004847.01 | MsG0480021587.01 | 0.817405 | 3.354587e-52 | 2.440801e-49 |
| MsG0180004847.01 | MsG0580024715.01 | 0.867507 | 1.300342e-65 | 4.551043e-62 |
| MsG0180004847.01 | MsG0580026222.01 | 0.823108 | 1.654107e-53 | 1.411102e-50 |
| MsG0180004847.01 | MsG0580026276.01 | 0.810930 | 9.022070e-51 | 5.510773e-48 |
| MsG0180004847.01 | MsG0580026303.01 | 0.815484 | 9.028604e-52 | 6.233111e-49 |
| MsG0180004847.01 | MsG0580026631.01 | 0.834149 | 3.541896e-56 | 4.166424e-53 |
| MsG0180004847.01 | MsG0580027332.01 | 0.834346 | 3.161109e-56 | 3.741148e-53 |
| MsG0180004847.01 | MsG0580028170.01 | 0.801391 | 9.207228e-49 | 4.391730e-46 |
| MsG0180004847.01 | MsG0580029381.01 | 0.828137 | 1.062483e-54 | 1.046879e-51 |
| MsG0180004847.01 | MsG0680031380.01 | 0.806364 | 8.525805e-50 | 4.618763e-47 |
| MsG0180004847.01 | MsG0680031896.01 | 0.802442 | 5.600771e-49 | 2.743786e-46 |
| MsG0180004847.01 | MsG0680033758.01 | 0.806298 | 8.803477e-50 | 4.761278e-47 |
| MsG0180004847.01 | MsG0680034565.01 | 0.814653 | 1.380764e-51 | 9.318696e-49 |
| MsG0180004847.01 | MsG0780036498.01 | 0.810916 | 9.085810e-51 | 5.547483e-48 |
| MsG0180004847.01 | MsG0780037606.01 | 0.826139 | 3.195703e-54 | 2.971594e-51 |
| MsG0180004847.01 | MsG0780039282.01 | 0.841193 | 5.513097e-58 | 8.038596e-55 |
| MsG0180004847.01 | MsG0880044644.01 | 0.813460 | 2.531314e-51 | 1.654174e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004847.01.T01 | MTR_8g098925 | 86.111 | 72 | 10 | 0 | 1 | 72 | 91 | 162 | 8.65e-40 | 130 |
| MsG0180004847.01.T01 | MTR_2g088140 | 92.308 | 65 | 5 | 0 | 38 | 102 | 51 | 115 | 6.13e-39 | 127 |
| MsG0180004847.01.T01 | MTR_5g022720 | 78.378 | 74 | 16 | 0 | 22 | 95 | 1 | 74 | 2.25e-35 | 122 |
| MsG0180004847.01.T01 | MTR_4g064590 | 51.887 | 106 | 45 | 3 | 9 | 113 | 62 | 162 | 1.41e-30 | 107 |
| MsG0180004847.01.T01 | MTR_6g089560 | 48.649 | 111 | 35 | 1 | 3 | 113 | 144 | 232 | 2.14e-29 | 105 |
| MsG0180004847.01.T01 | MTR_0093s0070 | 85.965 | 57 | 8 | 0 | 1 | 57 | 149 | 205 | 8.53e-29 | 103 |
| MsG0180004847.01.T01 | MTR_5g037920 | 54.455 | 101 | 44 | 2 | 1 | 100 | 124 | 223 | 6.22e-28 | 102 |
| MsG0180004847.01.T01 | MTR_2g027020 | 44.643 | 112 | 58 | 1 | 1 | 112 | 54 | 161 | 1.67e-26 | 97.4 |
| MsG0180004847.01.T01 | MTR_7g013520 | 50.000 | 114 | 30 | 2 | 2 | 113 | 95 | 183 | 3.40e-26 | 96.3 |
| MsG0180004847.01.T01 | MTR_5g083510 | 53.933 | 89 | 37 | 1 | 25 | 113 | 8 | 92 | 3.92e-26 | 93.6 |
| MsG0180004847.01.T01 | MTR_4g029360 | 61.644 | 73 | 28 | 0 | 3 | 75 | 155 | 227 | 3.72e-24 | 92.4 |
| MsG0180004847.01.T01 | MTR_2g055490 | 45.045 | 111 | 28 | 1 | 3 | 113 | 35 | 112 | 3.05e-23 | 87.0 |
| MsG0180004847.01.T01 | MTR_5g075610 | 50.633 | 79 | 37 | 1 | 1 | 79 | 21 | 97 | 5.66e-23 | 85.9 |
| MsG0180004847.01.T01 | MTR_1g103580 | 57.353 | 68 | 29 | 0 | 45 | 112 | 178 | 245 | 5.62e-22 | 87.4 |
| MsG0180004847.01.T01 | MTR_6g045427 | 66.071 | 56 | 19 | 0 | 7 | 62 | 177 | 232 | 9.96e-21 | 83.6 |
| MsG0180004847.01.T01 | MTR_1g088650 | 50.000 | 78 | 39 | 0 | 1 | 78 | 112 | 189 | 1.80e-20 | 82.0 |
| MsG0180004847.01.T01 | MTR_6g034915 | 39.823 | 113 | 51 | 1 | 1 | 113 | 81 | 176 | 2.93e-19 | 78.6 |
| MsG0180004847.01.T01 | MTR_6g060050 | 46.939 | 98 | 47 | 2 | 16 | 110 | 54 | 149 | 6.27e-19 | 77.0 |
| MsG0180004847.01.T01 | MTR_4g128080 | 55.385 | 65 | 29 | 0 | 26 | 90 | 5 | 69 | 2.61e-18 | 73.2 |
| MsG0180004847.01.T01 | MTR_1g017840 | 62.264 | 53 | 20 | 0 | 3 | 55 | 106 | 158 | 4.56e-18 | 75.1 |
| MsG0180004847.01.T01 | MTR_1g057740 | 50.000 | 80 | 40 | 0 | 32 | 111 | 60 | 139 | 5.82e-18 | 74.3 |
| MsG0180004847.01.T01 | MTR_5g065870 | 60.000 | 55 | 22 | 0 | 59 | 113 | 107 | 161 | 3.98e-17 | 72.4 |
| MsG0180004847.01.T01 | MTR_8g022750 | 50.000 | 70 | 35 | 0 | 4 | 73 | 217 | 286 | 7.67e-17 | 73.9 |
| MsG0180004847.01.T01 | MTR_8g028785 | 46.835 | 79 | 42 | 0 | 4 | 82 | 287 | 365 | 1.05e-16 | 74.3 |
| MsG0180004847.01.T01 | MTR_3g017480 | 51.351 | 74 | 36 | 0 | 38 | 111 | 46 | 119 | 1.25e-16 | 70.1 |
| MsG0180004847.01.T01 | MTR_7g063280 | 39.744 | 78 | 47 | 0 | 1 | 78 | 98 | 175 | 1.78e-16 | 71.2 |
| MsG0180004847.01.T01 | MTR_1g062840 | 46.377 | 69 | 37 | 0 | 2 | 70 | 7 | 75 | 4.00e-16 | 68.2 |
| MsG0180004847.01.T01 | MTR_3g010950 | 50.000 | 76 | 38 | 0 | 38 | 113 | 59 | 134 | 6.18e-16 | 68.9 |
| MsG0180004847.01.T01 | MTR_8g036690 | 40.230 | 87 | 34 | 2 | 6 | 92 | 2 | 70 | 7.72e-16 | 67.0 |
| MsG0180004847.01.T01 | MTR_3g116210 | 45.455 | 66 | 36 | 0 | 1 | 66 | 123 | 188 | 3.35e-14 | 65.5 |
| MsG0180004847.01.T01 | MTR_5g085210 | 38.298 | 94 | 42 | 1 | 1 | 94 | 145 | 222 | 3.76e-14 | 66.2 |
| MsG0180004847.01.T01 | MTR_2g059470 | 48.276 | 58 | 30 | 0 | 1 | 58 | 26 | 83 | 4.88e-14 | 62.4 |
| MsG0180004847.01.T01 | MTR_1g052310 | 42.574 | 101 | 50 | 3 | 18 | 110 | 21 | 121 | 1.08e-13 | 62.8 |
| MsG0180004847.01.T01 | MTR_4g098670 | 41.250 | 80 | 47 | 0 | 31 | 110 | 17 | 96 | 1.55e-13 | 61.6 |
| MsG0180004847.01.T01 | MTR_5g031620 | 35.577 | 104 | 60 | 1 | 10 | 113 | 126 | 222 | 1.74e-13 | 64.3 |
| MsG0180004847.01.T01 | MTR_6g016265 | 45.902 | 61 | 33 | 0 | 1 | 61 | 65 | 125 | 2.32e-13 | 62.0 |
| MsG0180004847.01.T01 | MTR_5g094290 | 60.000 | 55 | 22 | 0 | 56 | 110 | 34 | 88 | 4.85e-13 | 63.5 |
| MsG0180004847.01.T01 | MTR_3g065120 | 60.000 | 45 | 18 | 0 | 69 | 113 | 51 | 95 | 5.07e-13 | 60.5 |
| MsG0180004847.01.T01 | MTR_1g034430 | 45.902 | 61 | 33 | 0 | 1 | 61 | 121 | 181 | 5.08e-13 | 62.4 |
| MsG0180004847.01.T01 | MTR_8g059135 | 40.580 | 69 | 41 | 0 | 1 | 69 | 54 | 122 | 5.72e-13 | 60.8 |
| MsG0180004847.01.T01 | MTR_5g042170 | 63.636 | 44 | 16 | 0 | 70 | 113 | 77 | 120 | 6.42e-13 | 60.8 |
| MsG0180004847.01.T01 | MTR_1g069475 | 48.214 | 56 | 29 | 0 | 1 | 56 | 190 | 245 | 7.34e-13 | 62.8 |
| MsG0180004847.01.T01 | MTR_6g453270 | 54.717 | 53 | 24 | 0 | 1 | 53 | 326 | 378 | 1.16e-12 | 63.2 |
| MsG0180004847.01.T01 | MTR_3g071490 | 55.769 | 52 | 23 | 0 | 62 | 113 | 9 | 60 | 1.46e-12 | 58.2 |
| MsG0180004847.01.T01 | MTR_3g090920 | 30.909 | 110 | 60 | 2 | 3 | 112 | 15 | 108 | 1.47e-12 | 59.3 |
| MsG0180004847.01.T01 | MTR_8g099580 | 50.909 | 55 | 27 | 0 | 4 | 58 | 235 | 289 | 1.85e-12 | 62.4 |
| MsG0180004847.01.T01 | MTR_3g465840 | 32.632 | 95 | 62 | 1 | 1 | 93 | 1 | 95 | 2.09e-12 | 60.1 |
| MsG0180004847.01.T01 | MTR_5g083200 | 38.028 | 71 | 44 | 0 | 1 | 71 | 124 | 194 | 3.61e-12 | 60.5 |
| MsG0180004847.01.T01 | MTR_0002s1220 | 38.028 | 71 | 44 | 0 | 1 | 71 | 124 | 194 | 3.61e-12 | 60.5 |
| MsG0180004847.01.T01 | MTR_3g111390 | 38.028 | 71 | 44 | 0 | 1 | 71 | 124 | 194 | 3.65e-12 | 60.5 |
| MsG0180004847.01.T01 | MTR_1g070820 | 38.028 | 71 | 44 | 0 | 1 | 71 | 124 | 194 | 3.65e-12 | 60.5 |
| MsG0180004847.01.T01 | MTR_8g005560 | 45.614 | 57 | 31 | 0 | 22 | 78 | 1 | 57 | 4.57e-12 | 58.9 |
| MsG0180004847.01.T01 | MTR_4g008270 | 50.000 | 56 | 28 | 0 | 1 | 56 | 36 | 91 | 5.55e-12 | 57.8 |
| MsG0180004847.01.T01 | MTR_6g007540 | 56.364 | 55 | 24 | 0 | 47 | 101 | 23 | 77 | 7.57e-12 | 58.5 |
| MsG0180004847.01.T01 | MTR_2g047100 | 44.776 | 67 | 36 | 1 | 9 | 74 | 36 | 102 | 8.96e-12 | 57.4 |
| MsG0180004847.01.T01 | MTR_1g045340 | 36.620 | 71 | 45 | 0 | 1 | 71 | 110 | 180 | 1.00e-11 | 58.9 |
| MsG0180004847.01.T01 | MTR_5g069700 | 44.262 | 61 | 34 | 0 | 1 | 61 | 7 | 67 | 1.98e-11 | 55.8 |
| MsG0180004847.01.T01 | MTR_6g023630 | 64.286 | 42 | 13 | 2 | 1 | 41 | 15 | 55 | 8.35e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATTGATGTTACAGAATT+TGG | 0.136846 | 1:-83519126 | MsG0180004847.01.T01:CDS |
| ATCAATCCAACGATGGTTAA+AGG | 0.269957 | 1:+83519140 | None:intergenic |
| GAGGTAGCTCACACCCTTGC+TGG | 0.335947 | 1:-83519021 | MsG0180004847.01.T01:CDS |
| AGGTACACGATAATAAATAT+TGG | 0.337842 | 1:+83518975 | None:intergenic |
| ACTCTAAGGTCGAGTTTAAC+AGG | 0.344267 | 1:-83519056 | MsG0180004847.01.T01:CDS |
| CACAAACTTCTCAAACTCTA+AGG | 0.397546 | 1:-83519070 | MsG0180004847.01.T01:CDS |
| CATGTCCCTTAGCCATTGTA+AGG | 0.412641 | 1:+83519212 | None:intergenic |
| GGTACACGATAATAAATATT+GGG | 0.428046 | 1:+83518976 | None:intergenic |
| AGGTAGCTCACACCCTTGCT+GGG | 0.481440 | 1:-83519020 | MsG0180004847.01.T01:CDS |
| CAGATGCCTTTAACCATCGT+TGG | 0.488281 | 1:-83519146 | MsG0180004847.01.T01:CDS |
| TCCATGCCTTACAATGGCTA+AGG | 0.495478 | 1:-83519218 | MsG0180004847.01.T01:CDS |
| CCCTTAGCCATTGTAAGGCA+TGG | 0.508781 | 1:+83519217 | None:intergenic |
| TGACAACGTCGATTTCGTCT+TGG | 0.515739 | 1:-83519184 | MsG0180004847.01.T01:CDS |
| AGTTTGTGTTAAAGAGACTC+TGG | 0.543874 | 1:+83519085 | None:intergenic |
| TAACAGGAGACAAGCAAATG+AGG | 0.544166 | 1:-83519040 | MsG0180004847.01.T01:CDS |
| TCTGGCATGCTGAAATCACT+TGG | 0.580643 | 1:+83519103 | None:intergenic |
| GCTTATTCCATGCCTTACAA+TGG | 0.586368 | 1:-83519224 | MsG0180004847.01.T01:CDS |
| GTTGCCGTAGGAGAAGCTCT+AGG | 0.600043 | 1:-83519246 | MsG0180004847.01.T01:CDS |
| ATTGGGACTAGCTGATAATG+TGG | 0.608869 | 1:+83518993 | None:intergenic |
| CCATGCCTTACAATGGCTAA+GGG | 0.609268 | 1:-83519217 | MsG0180004847.01.T01:CDS |
| TAAGCCTAGAGCTTCTCCTA+CGG | 0.612405 | 1:+83519242 | None:intergenic |
| GGTAGCTCACACCCTTGCTG+GGG | 0.620762 | 1:-83519019 | MsG0180004847.01.T01:CDS |
| TAATGTGGCAACCCCAGCAA+GGG | 0.641468 | 1:+83519008 | None:intergenic |
| ATAATGTGGCAACCCCAGCA+AGG | 0.641592 | 1:+83519007 | None:intergenic |
| CTGTAACATCAATCCAACGA+TGG | 0.667612 | 1:+83519133 | None:intergenic |
| ATCATGTGTTCTGTTGCCGT+AGG | 0.675346 | 1:-83519258 | MsG0180004847.01.T01:CDS |
| ATAAGAGAGTTTATACAACG+AGG | 0.814797 | 1:+83518955 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGGTACACGATAATAAATAT+TGG | + | Chr1:83519219-83519238 | None:intergenic | 25.0% |
| ! | GGTACACGATAATAAATATT+GGG | + | Chr1:83519218-83519237 | None:intergenic | 25.0% |
| ATAAGAGAGTTTATACAACG+AGG | + | Chr1:83519239-83519258 | None:intergenic | 30.0% | |
| TGGATTGATGTTACAGAATT+TGG | - | Chr1:83519065-83519084 | MsG0180004847.01.T01:CDS | 30.0% | |
| AGTTTGTGTTAAAGAGACTC+TGG | + | Chr1:83519109-83519128 | None:intergenic | 35.0% | |
| ATCAATCCAACGATGGTTAA+AGG | + | Chr1:83519054-83519073 | None:intergenic | 35.0% | |
| CACAAACTTCTCAAACTCTA+AGG | - | Chr1:83519121-83519140 | MsG0180004847.01.T01:CDS | 35.0% | |
| CTGTAACATCAATCCAACGA+TGG | + | Chr1:83519061-83519080 | None:intergenic | 40.0% | |
| GCTTATTCCATGCCTTACAA+TGG | - | Chr1:83518967-83518986 | MsG0180004847.01.T01:CDS | 40.0% | |
| TAACAGGAGACAAGCAAATG+AGG | - | Chr1:83519151-83519170 | MsG0180004847.01.T01:CDS | 40.0% | |
| !! | ACTCTAAGGTCGAGTTTAAC+AGG | - | Chr1:83519135-83519154 | MsG0180004847.01.T01:CDS | 40.0% |
| !! | ATTGGGACTAGCTGATAATG+TGG | + | Chr1:83519201-83519220 | None:intergenic | 40.0% |
| CAGATGCCTTTAACCATCGT+TGG | - | Chr1:83519045-83519064 | MsG0180004847.01.T01:CDS | 45.0% | |
| CATGTCCCTTAGCCATTGTA+AGG | + | Chr1:83518982-83519001 | None:intergenic | 45.0% | |
| CCATGCCTTACAATGGCTAA+GGG | - | Chr1:83518974-83518993 | MsG0180004847.01.T01:CDS | 45.0% | |
| TAAGCCTAGAGCTTCTCCTA+CGG | + | Chr1:83518952-83518971 | None:intergenic | 45.0% | |
| TCCATGCCTTACAATGGCTA+AGG | - | Chr1:83518973-83518992 | MsG0180004847.01.T01:CDS | 45.0% | |
| TCTGGCATGCTGAAATCACT+TGG | + | Chr1:83519091-83519110 | None:intergenic | 45.0% | |
| !! | TGACAACGTCGATTTCGTCT+TGG | - | Chr1:83519007-83519026 | MsG0180004847.01.T01:CDS | 45.0% |
| ATAATGTGGCAACCCCAGCA+AGG | + | Chr1:83519187-83519206 | None:intergenic | 50.0% | |
| CCCTTAGCCATTGTAAGGCA+TGG | + | Chr1:83518977-83518996 | None:intergenic | 50.0% | |
| TAATGTGGCAACCCCAGCAA+GGG | + | Chr1:83519186-83519205 | None:intergenic | 50.0% | |
| AGGTAGCTCACACCCTTGCT+GGG | - | Chr1:83519171-83519190 | MsG0180004847.01.T01:CDS | 55.0% | |
| GTTGCCGTAGGAGAAGCTCT+AGG | - | Chr1:83518945-83518964 | MsG0180004847.01.T01:CDS | 55.0% | |
| GAGGTAGCTCACACCCTTGC+TGG | - | Chr1:83519170-83519189 | MsG0180004847.01.T01:CDS | 60.0% | |
| GGTAGCTCACACCCTTGCTG+GGG | - | Chr1:83519172-83519191 | MsG0180004847.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 83518936 | 83519277 | 83518936 | ID=MsG0180004847.01;Name=MsG0180004847.01 |
| Chr1 | mRNA | 83518936 | 83519277 | 83518936 | ID=MsG0180004847.01.T01;Parent=MsG0180004847.01;Name=MsG0180004847.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|113 |
| Chr1 | exon | 83518936 | 83519277 | 83518936 | ID=MsG0180004847.01.T01:exon:27683;Parent=MsG0180004847.01.T01 |
| Chr1 | CDS | 83518936 | 83519277 | 83518936 | ID=MsG0180004847.01.T01:cds;Parent=MsG0180004847.01.T01 |
| Gene Sequence |
| Protein sequence |
