Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034565.01.T01 | RHN76464.1 | 81.481 | 54 | 3 | 1 | 1 | 54 | 4 | 50 | 2.84E-19 | 82.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034565.01.T01 | A0A396JH39 | 81.481 | 54 | 3 | 1 | 1 | 54 | 4 | 50 | 1.36e-19 | 82.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047869.01 | MsG0680034565.01 | 0.804085 | 2.559018e-49 | 1.307524e-46 |
| MsG0080047959.01 | MsG0680034565.01 | 0.800260 | 1.566457e-48 | 7.261042e-46 |
| MsG0080048874.01 | MsG0680034565.01 | 0.819754 | 9.837322e-53 | 7.637208e-50 |
| MsG0180000470.01 | MsG0680034565.01 | 0.839984 | 1.142598e-57 | 1.604962e-54 |
| MsG0180000650.01 | MsG0680034565.01 | 0.816536 | 5.257668e-52 | 3.735424e-49 |
| MsG0180001063.01 | MsG0680034565.01 | 0.817731 | 2.831698e-52 | 2.078926e-49 |
| MsG0180001410.01 | MsG0680034565.01 | 0.812965 | 3.250340e-51 | 2.095464e-48 |
| MsG0180001971.01 | MsG0680034565.01 | 0.804505 | 2.091557e-49 | 1.080233e-46 |
| MsG0180002008.01 | MsG0680034565.01 | 0.811289 | 7.541542e-51 | 4.649745e-48 |
| MsG0180002089.01 | MsG0680034565.01 | 0.811689 | 6.175172e-51 | 3.847693e-48 |
| MsG0180002234.01 | MsG0680034565.01 | 0.808608 | 2.849151e-50 | 1.636233e-47 |
| MsG0180002563.01 | MsG0680034565.01 | 0.807189 | 5.708780e-50 | 3.159174e-47 |
| MsG0180002680.01 | MsG0680034565.01 | 0.824084 | 9.777835e-54 | 8.573678e-51 |
| MsG0180002842.01 | MsG0680034565.01 | 0.820720 | 5.908307e-53 | 4.711515e-50 |
| MsG0180003669.01 | MsG0680034565.01 | 0.811510 | 6.753866e-51 | 4.188822e-48 |
| MsG0180004264.01 | MsG0680034565.01 | 0.830272 | 3.224486e-55 | 3.380968e-52 |
| MsG0180004765.01 | MsG0680034565.01 | 0.808274 | 3.356289e-50 | 1.910931e-47 |
| MsG0180004847.01 | MsG0680034565.01 | 0.814653 | 1.380764e-51 | 9.318696e-49 |
| MsG0180006257.01 | MsG0680034565.01 | 0.821471 | 3.967221e-53 | 3.230470e-50 |
| MsG0280006325.01 | MsG0680034565.01 | 0.829012 | 6.530627e-55 | 6.600293e-52 |
| MsG0280011163.01 | MsG0680034565.01 | 0.823716 | 1.192509e-53 | 1.035049e-50 |
| MsG0380012007.01 | MsG0680034565.01 | 0.809501 | 1.833719e-50 | 1.078439e-47 |
| MsG0380012009.01 | MsG0680034565.01 | 0.818657 | 1.747952e-52 | 1.316277e-49 |
| MsG0380014251.01 | MsG0680034565.01 | 0.812742 | 3.638296e-51 | 2.331900e-48 |
| MsG0380014573.01 | MsG0680034565.01 | 0.808354 | 3.226904e-50 | 1.841135e-47 |
| MsG0380014894.01 | MsG0680034565.01 | 0.801246 | 9.858959e-49 | 4.685604e-46 |
| MsG0680030882.01 | MsG0680034565.01 | 0.801112 | 1.050047e-48 | 4.973687e-46 |
| MsG0680030886.01 | MsG0680034565.01 | 0.804937 | 1.699556e-49 | 8.877191e-47 |
| MsG0680031210.01 | MsG0680034565.01 | 0.803542 | 3.316404e-49 | 1.671126e-46 |
| MsG0680031355.01 | MsG0680034565.01 | 0.818682 | 1.725976e-52 | 1.300630e-49 |
| MsG0680031379.01 | MsG0680034565.01 | 0.825863 | 3.717749e-54 | 3.430175e-51 |
| MsG0680032157.01 | MsG0680034565.01 | 0.823207 | 1.568455e-53 | 1.341690e-50 |
| MsG0680032158.01 | MsG0680034565.01 | 0.806786 | 6.946915e-50 | 3.804316e-47 |
| MsG0680032355.01 | MsG0680034565.01 | 0.802537 | 5.352337e-49 | 2.628450e-46 |
| MsG0680032801.01 | MsG0680034565.01 | 0.816234 | 6.141645e-52 | 4.327720e-49 |
| MsG0680033228.01 | MsG0680034565.01 | 0.806200 | 9.233491e-50 | 4.981498e-47 |
| MsG0680033412.01 | MsG0680034565.01 | 0.800054 | 1.725646e-48 | 7.957046e-46 |
| MsG0680033626.01 | MsG0680034565.01 | 0.817041 | 4.051428e-52 | 2.918580e-49 |
| MsG0680034464.01 | MsG0680034565.01 | 0.802582 | 5.239813e-49 | 2.576096e-46 |
| MsG0680034565.01 | MsG0680035444.01 | 0.807114 | 5.920855e-50 | 3.270013e-47 |
| MsG0680034565.01 | MsG0780035948.01 | 0.817629 | 2.985967e-52 | 2.185914e-49 |
| MsG0680034565.01 | MsG0780036080.01 | 0.814372 | 1.592861e-51 | 1.066919e-48 |
| MsG0680034565.01 | MsG0780036201.01 | 0.800713 | 1.266632e-48 | 5.938807e-46 |
| MsG0680034565.01 | MsG0780037457.01 | 0.802902 | 4.499464e-49 | 2.230335e-46 |
| MsG0680034565.01 | MsG0780037592.01 | 0.812586 | 3.935306e-51 | 2.511937e-48 |
| MsG0680034565.01 | MsG0780039631.01 | 0.829091 | 6.247369e-55 | 6.329294e-52 |
| MsG0680034565.01 | MsG0780039867.01 | -0.816314 | 5.895114e-52 | 4.163271e-49 |
| MsG0680034565.01 | MsG0780040576.01 | -0.823435 | 1.387305e-53 | 1.194357e-50 |
| MsG0680034565.01 | MsG0780040596.01 | 0.811985 | 5.322390e-51 | 3.342763e-48 |
| MsG0680034565.01 | MsG0780040704.01 | 0.805541 | 1.270313e-49 | 6.738131e-47 |
| MsG0680034565.01 | MsG0880041836.01 | 0.801553 | 8.526984e-49 | 4.083994e-46 |
| MsG0680034565.01 | MsG0880042161.01 | 0.815954 | 7.094808e-52 | 4.961074e-49 |
| MsG0680034565.01 | MsG0880042454.01 | 0.809624 | 1.726208e-50 | 1.018597e-47 |
| MsG0680034565.01 | MsG0880042966.01 | 0.804356 | 2.246683e-49 | 1.155976e-46 |
| MsG0680034565.01 | MsG0880044085.01 | 0.817321 | 3.502892e-52 | 2.542800e-49 |
| MsG0680034565.01 | MsG0880044574.01 | 0.812949 | 3.276618e-51 | 2.111507e-48 |
| MsG0680034565.01 | MsG0880044821.01 | 0.810189 | 1.304484e-50 | 7.811541e-48 |
| MsG0480018664.01 | MsG0680034565.01 | 0.802568 | 5.273182e-49 | 2.591578e-46 |
| MsG0480018964.01 | MsG0680034565.01 | 0.805736 | 1.156097e-49 | 6.163745e-47 |
| MsG0480019337.01 | MsG0680034565.01 | 0.823058 | 1.699390e-53 | 1.447695e-50 |
| MsG0480019435.01 | MsG0680034565.01 | 0.813250 | 2.815452e-51 | 1.829097e-48 |
| MsG0480020634.01 | MsG0680034565.01 | 0.807850 | 4.131967e-50 | 2.326199e-47 |
| MsG0480021276.01 | MsG0680034565.01 | 0.802284 | 6.035934e-49 | 2.945221e-46 |
| MsG0480021536.01 | MsG0680034565.01 | 0.804560 | 2.037209e-49 | 1.053689e-46 |
| MsG0480021558.01 | MsG0680034565.01 | 0.801685 | 8.014827e-49 | 3.851357e-46 |
| MsG0480021853.01 | MsG0680034565.01 | 0.807626 | 4.610376e-50 | 2.580477e-47 |
| MsG0480021925.01 | MsG0680034565.01 | 0.806927 | 6.485630e-50 | 3.564694e-47 |
| MsG0480022259.01 | MsG0680034565.01 | 0.835461 | 1.655212e-56 | 2.025490e-53 |
| MsG0480022262.01 | MsG0680034565.01 | 0.827914 | 1.202248e-54 | 1.176856e-51 |
| MsG0480022615.01 | MsG0680034565.01 | -0.808362 | 3.215281e-50 | 1.834857e-47 |
| MsG0480023654.01 | MsG0680034565.01 | 0.831901 | 1.283733e-55 | 1.412491e-52 |
| MsG0580024038.01 | MsG0680034565.01 | 0.801323 | 9.503809e-49 | 4.525575e-46 |
| MsG0580024363.01 | MsG0680034565.01 | -0.809686 | 1.673404e-50 | 9.890397e-48 |
| MsG0580025448.01 | MsG0680034565.01 | 0.803712 | 3.058583e-49 | 1.547798e-46 |
| MsG0380015566.01 | MsG0680034565.01 | 0.812047 | 5.161095e-51 | 3.246758e-48 |
| MsG0380015720.01 | MsG0680034565.01 | 0.814482 | 1.506858e-51 | 1.012315e-48 |
| MsG0380016004.01 | MsG0680034565.01 | 0.801382 | 9.242895e-49 | 4.407905e-46 |
| MsG0380016475.01 | MsG0680034565.01 | 0.800633 | 1.314922e-48 | 6.153099e-46 |
| MsG0380016910.01 | MsG0680034565.01 | 0.809695 | 1.666227e-50 | 9.850188e-48 |
| MsG0380017060.01 | MsG0680034565.01 | 0.807428 | 5.079387e-50 | 2.828452e-47 |
| MsG0380017343.01 | MsG0680034565.01 | 0.815633 | 8.366298e-52 | 5.799435e-49 |
| MsG0380017680.01 | MsG0680034565.01 | 0.805255 | 1.457804e-49 | 7.676160e-47 |
| MsG0480018163.01 | MsG0680034565.01 | 0.805493 | 1.300167e-49 | 6.888206e-47 |
| MsG0480018236.01 | MsG0680034565.01 | 0.812193 | 4.795822e-51 | 3.029288e-48 |
| MsG0580027766.01 | MsG0680034565.01 | 0.807766 | 4.304955e-50 | 2.418324e-47 |
| MsG0580027829.01 | MsG0680034565.01 | 0.825269 | 5.140197e-54 | 4.662800e-51 |
| MsG0580027833.01 | MsG0680034565.01 | 0.801943 | 7.093097e-49 | 3.430900e-46 |
| MsG0580027922.01 | MsG0680034565.01 | 0.825802 | 3.842776e-54 | 3.539324e-51 |
| MsG0580028170.01 | MsG0680034565.01 | 0.805231 | 1.475281e-49 | 7.763477e-47 |
| MsG0580028527.01 | MsG0680034565.01 | 0.810088 | 1.371237e-50 | 8.189967e-48 |
| MsG0280006769.01 | MsG0680034565.01 | 0.800428 | 1.448247e-48 | 6.741863e-46 |
| MsG0280007335.01 | MsG0680034565.01 | 0.804477 | 2.120407e-49 | 1.094350e-46 |
| MsG0280008615.01 | MsG0680034565.01 | 0.803986 | 2.682869e-49 | 1.367398e-46 |
| MsG0280008894.01 | MsG0680034565.01 | 0.801097 | 1.057241e-48 | 5.006120e-46 |
| MsG0280009297.01 | MsG0680034565.01 | 0.806307 | 8.766864e-50 | 4.742514e-47 |
| MsG0280009986.01 | MsG0680034565.01 | 0.801641 | 8.182700e-49 | 3.927727e-46 |
| MsG0280010443.01 | MsG0680034565.01 | 0.838867 | 2.228396e-57 | 3.023989e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680034565.01.T01 | MTR_0623s0010 | 81.481 | 54 | 3 | 1 | 1 | 54 | 99 | 145 | 5.86e-23 | 84.7 |
| MsG0680034565.01.T01 | MTR_4g056250 | 90.698 | 43 | 4 | 0 | 1 | 43 | 31 | 73 | 3.21e-22 | 80.5 |
| MsG0680034565.01.T01 | MTR_0623s0020 | 80.769 | 52 | 3 | 1 | 1 | 52 | 44 | 88 | 1.46e-21 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAATGACACCATTCTTCT+TGG | 0.249236 | 6:+90378180 | None:intergenic |
| GGAGGAGAGTGAACGAAATT+TGG | 0.263625 | 6:-90377949 | MsG0680034565.01.T01:three_prime_UTR |
| GATGATTTGTCTGCTTGTTT+TGG | 0.299398 | 6:-90377918 | MsG0680034565.01.T01:three_prime_UTR |
| TATATATGTTACCATATTAT+AGG | 0.299435 | 6:-90377795 | MsG0680034565.01.T01:three_prime_UTR |
| ATAAACATCAAAATCTTCTA+TGG | 0.354137 | 6:+90377847 | None:intergenic |
| TAAATGTATAGCCTATAATA+TGG | 0.404365 | 6:+90377784 | None:intergenic |
| ACTAAACGAGATTTCAAAGT+TGG | 0.432451 | 6:-90378342 | MsG0680034565.01.T01:CDS |
| TAAGAGAAATCCAAGAAGAA+TGG | 0.512769 | 6:-90378190 | MsG0680034565.01.T01:CDS |
| ATTCAGGTGGAGGATTCAAG+TGG | 0.634957 | 6:-90377970 | MsG0680034565.01.T01:intron |
| CAGGTGGAGGATTCAAGTGG+AGG | 0.641521 | 6:-90377967 | MsG0680034565.01.T01:three_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAATATCATATAACTTTT+AGG | + | Chr6:90377948-90377967 | None:intergenic | 10.0% |
| !!! | TATATGATATTTTTATGATA+AGG | - | Chr6:90377954-90377973 | MsG0680034565.01.T01:three_prime_UTR | 10.0% |
| !! | AATATAGTATTATTGCTTAA+TGG | - | Chr6:90378087-90378106 | MsG0680034565.01.T01:intron | 15.0% |
| !! | ATTAAGTTTATTAAGTAACA+TGG | - | Chr6:90377814-90377833 | MsG0680034565.01.T01:three_prime_UTR | 15.0% |
| !! | TATATATGTTACCATATTAT+AGG | - | Chr6:90378300-90378319 | MsG0680034565.01.T01:intron | 15.0% |
| !!! | TACATACATTTTTTATGATA+TGG | - | Chr6:90378352-90378371 | MsG0680034565.01.T01:CDS | 15.0% |
| !! | ATAAACATCAAAATCTTCTA+TGG | + | Chr6:90378251-90378270 | None:intergenic | 20.0% |
| !! | TAAATGTATAGCCTATAATA+TGG | + | Chr6:90378314-90378333 | None:intergenic | 20.0% |
| !!! | ATGATATTTTTATGATAAGG+TGG | - | Chr6:90377957-90377976 | MsG0680034565.01.T01:three_prime_UTR | 20.0% |
| !!! | TGATATTTTTATGATAAGGT+GGG | - | Chr6:90377958-90377977 | MsG0680034565.01.T01:three_prime_UTR | 20.0% |
| !! | ATCAATATATTTGCTAGAAG+TGG | - | Chr6:90377991-90378010 | MsG0680034565.01.T01:intron | 25.0% |
| !!! | GTGTTTTTTGGTATATATTC+AGG | - | Chr6:90378109-90378128 | MsG0680034565.01.T01:intron | 25.0% |
| !!! | TATTGCTTAATGGTGTTTTT+TGG | - | Chr6:90378097-90378116 | MsG0680034565.01.T01:intron | 25.0% |
| !!! | TTTTTTGGTATATATTCAGG+TGG | - | Chr6:90378112-90378131 | MsG0680034565.01.T01:intron | 25.0% |
| ACTAAACGAGATTTCAAAGT+TGG | - | Chr6:90377753-90377772 | MsG0680034565.01.T01:three_prime_UTR | 30.0% | |
| TAAGAGAAATCCAAGAAGAA+TGG | - | Chr6:90377905-90377924 | MsG0680034565.01.T01:three_prime_UTR | 30.0% | |
| !!! | CTGCTTGTTTTGGATAAAAA+AGG | - | Chr6:90378187-90378206 | MsG0680034565.01.T01:CDS | 30.0% |
| TGAAATGACACCATTCTTCT+TGG | + | Chr6:90377918-90377937 | None:intergenic | 35.0% | |
| TTTGGTATATATTCAGGTGG+AGG | - | Chr6:90378115-90378134 | MsG0680034565.01.T01:intron | 35.0% | |
| !! | GATGATTTGTCTGCTTGTTT+TGG | - | Chr6:90378177-90378196 | MsG0680034565.01.T01:CDS | 35.0% |
| ATTCAGGTGGAGGATTCAAG+TGG | - | Chr6:90378125-90378144 | MsG0680034565.01.T01:intron | 45.0% | |
| GGAGGAGAGTGAACGAAATT+TGG | - | Chr6:90378146-90378165 | MsG0680034565.01.T01:exon | 45.0% | |
| CAGGTGGAGGATTCAAGTGG+AGG | - | Chr6:90378128-90378147 | MsG0680034565.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 90377730 | 90378387 | 90377730 | ID=MsG0680034565.01;Name=MsG0680034565.01 |
| Chr6 | mRNA | 90377730 | 90378387 | 90377730 | ID=MsG0680034565.01.T01;Parent=MsG0680034565.01;Name=MsG0680034565.01.T01;_AED=0.34;_eAED=0.34;_QI=0|0.5|0.66|1|0|0|3|272|54 |
| Chr6 | exon | 90377730 | 90377986 | 90377730 | ID=MsG0680034565.01.T01:exon:1366;Parent=MsG0680034565.01.T01 |
| Chr6 | exon | 90378142 | 90378255 | 90378142 | ID=MsG0680034565.01.T01:exon:1365;Parent=MsG0680034565.01.T01 |
| Chr6 | exon | 90378322 | 90378387 | 90378322 | ID=MsG0680034565.01.T01:exon:1364;Parent=MsG0680034565.01.T01 |
| Chr6 | CDS | 90378322 | 90378387 | 90378322 | ID=MsG0680034565.01.T01:cds;Parent=MsG0680034565.01.T01 |
| Chr6 | CDS | 90378157 | 90378255 | 90378157 | ID=MsG0680034565.01.T01:cds;Parent=MsG0680034565.01.T01 |
| Chr6 | three_prime_UTR | 90378142 | 90378156 | 90378142 | ID=MsG0680034565.01.T01:three_prime_utr;Parent=MsG0680034565.01.T01 |
| Chr6 | three_prime_UTR | 90377730 | 90377986 | 90377730 | ID=MsG0680034565.01.T01:three_prime_utr;Parent=MsG0680034565.01.T01 |
| Gene Sequence |
| Protein sequence |
