AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680034238.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680034238.01.T01 MTR_6g059760 84.038 213 33 1 1 212 1 213 3.54e-126 355
MsG0680034238.01.T01 MTR_6g059755 87.692 130 15 1 84 212 1 130 8.16e-79 232
MsG0680034238.01.T01 MTR_6g059650 52.055 219 90 9 1 212 1 211 5.71e-53 169
MsG0680034238.01.T01 MTR_6g065460 46.890 209 98 8 1 201 1 204 2.09e-44 147
MsG0680034238.01.T01 MTR_6g044780 42.727 220 115 8 1 212 1 217 3.37e-40 137
MsG0680034238.01.T01 MTR_6g045097 42.727 220 115 8 1 212 1 217 6.03e-40 136
MsG0680034238.01.T01 MTR_6g044810 42.202 218 115 8 1 210 1 215 2.23e-39 135
MsG0680034238.01.T01 MTR_6g059410 45.070 213 98 9 1 207 1 200 1.59e-38 132
MsG0680034238.01.T01 MTR_6g059530 44.393 214 99 10 1 207 1 201 1.61e-37 129
MsG0680034238.01.T01 MTR_6g045403 41.629 221 115 10 1 212 1 216 7.02e-37 129
MsG0680034238.01.T01 MTR_6g045433 38.865 229 116 9 1 212 1 222 5.02e-33 119
MsG0680034238.01.T01 MTR_3g463590 39.894 188 99 8 29 207 27 209 5.99e-30 110
MsG0680034238.01.T01 MTR_6g065460 38.647 207 94 7 1 201 1 180 6.60e-29 107
MsG0680034238.01.T01 MTR_6g066190 39.819 221 113 12 1 211 1 211 4.63e-27 103
MsG0680034238.01.T01 MTR_1g057110 37.931 203 103 9 1 199 1 184 5.34e-26 99.8
MsG0680034238.01.T01 MTR_6g009650 39.623 212 109 11 1 202 1 203 3.66e-25 98.2
MsG0680034238.01.T01 MTR_6g059730 37.321 209 118 7 1 201 1 204 5.82e-25 97.8
MsG0680034238.01.T01 MTR_6g009563 38.028 213 112 12 1 202 1 204 1.38e-21 88.6
MsG0680034238.01.T01 MTR_6g017225 35.648 216 116 11 1 201 1 208 1.62e-21 91.3
MsG0680034238.01.T01 MTR_6g066210 36.771 223 120 12 1 211 1 214 3.09e-21 87.8
MsG0680034238.01.T01 MTR_6g059680 37.436 195 105 10 18 200 18 207 1.33e-20 86.3
MsG0680034238.01.T01 MTR_1g035770 34.804 204 114 13 11 200 11 209 1.14e-14 70.5
MsG0680034238.01.T01 MTR_7g037410 33.140 172 98 8 29 189 27 192 3.00e-13 66.2
MsG0680034238.01.T01 MTR_6g078070 31.667 180 110 8 29 198 24 200 2.78e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATGATTTCATCTTTAC+TGG 0.216581 6:-84229038 MsG0680034238.01.T01:CDS
TGAGTAAGAAATACTTTATT+TGG 0.224891 6:-84229184 MsG0680034238.01.T01:CDS
GGTATTGGTGGTGGTGTTAA+TGG 0.238618 6:-84228918 MsG0680034238.01.T01:CDS
CCTGGTTTATGTAATAATAT+TGG 0.276379 6:-84228807 MsG0680034238.01.T01:CDS
TCCAAGTGGGTGGTAGTTAA+AGG 0.301512 6:-84228963 MsG0680034238.01.T01:CDS
ACAAATTGAGTAAAGGATAA+TGG 0.347391 6:+84229245 None:intergenic
GGTAAGTAATGTAGGGTTCA+TGG 0.354211 6:+84229307 None:intergenic
TATTCAAACTACAGATCTTC+CGG 0.354335 6:+84228686 None:intergenic
TATTTCTTACTCACAAGAAC+GGG 0.363139 6:+84229194 None:intergenic
ATTGAACCATGGGTAGGTAT+TGG 0.417221 6:-84228933 MsG0680034238.01.T01:CDS
TAGAGGGTGGTAAGTAATGT+AGG 0.427202 6:+84229299 None:intergenic
AATGGTAAGAGTATTATAGA+TGG 0.435603 6:-84228900 MsG0680034238.01.T01:CDS
ACCTTTAACTACCACCCACT+TGG 0.461157 6:+84228962 None:intergenic
GGGCAAAGAGGAAGAAATAG+AGG 0.480997 6:+84229282 None:intergenic
TCAACGAATACAACTTCAAA+GGG 0.482997 6:+84228729 None:intergenic
GTTGAAGATGCTCCTCGATC+CGG 0.487710 6:-84228705 MsG0680034238.01.T01:CDS
AAATACTTTATTTGGTCAGC+CGG 0.495640 6:-84229176 MsG0680034238.01.T01:CDS
TTGGGATAAATGTTAGTGGC+AGG 0.501980 6:+84229069 None:intergenic
ACCAGGTGTATCACTGGTGG+TGG 0.503174 6:+84228824 None:intergenic
TCTGTTGGGATAAATGTTAG+TGG 0.504722 6:+84229065 None:intergenic
TAATGGAAAGTAGGTGGCTA+GGG 0.507074 6:+84229262 None:intergenic
TTTAACTACCACCCACTTGG+AGG 0.510750 6:+84228965 None:intergenic
AGTAAAGGATAATGGAAAGT+AGG 0.512041 6:+84229253 None:intergenic
CAGTCCACCACCACTTTCAC+CGG 0.513171 6:+84229157 None:intergenic
TCAATTTGTTGAACAAGTGA+AGG 0.514281 6:-84229231 MsG0680034238.01.T01:CDS
ATCAACGAATACAACTTCAA+AGG 0.518113 6:+84228728 None:intergenic
GAACCATGGGTAGGTATTGG+TGG 0.518436 6:-84228930 MsG0680034238.01.T01:CDS
AGGTGGTTTCATTGAACCAT+GGG 0.524203 6:-84228943 MsG0680034238.01.T01:CDS
TTGTTCAACAAATTGAGTAA+AGG 0.544184 6:+84229238 None:intergenic
ATTTGGTCAGCCGGTGAAAG+TGG 0.544335 6:-84229167 MsG0680034238.01.T01:CDS
ACATAAACCAGGTGTATCAC+TGG 0.553319 6:+84228818 None:intergenic
AAAGCTTGCTGTACAACAAG+TGG 0.555218 6:+84229110 None:intergenic
ATAATGGAAAGTAGGTGGCT+AGG 0.557559 6:+84229261 None:intergenic
CCATGGGTAGGTATTGGTGG+TGG 0.565594 6:-84228927 MsG0680034238.01.T01:CDS
AAGGTGGTTTCATTGAACCA+TGG 0.574110 6:-84228944 MsG0680034238.01.T01:CDS
GTATTTCTTACTCACAAGAA+CGG 0.581176 6:+84229193 None:intergenic
TTGTTTAAGATTGAGAGAAG+TGG 0.588206 6:-84228876 MsG0680034238.01.T01:CDS
AGAGGGTGGTAAGTAATGTA+GGG 0.588469 6:+84229300 None:intergenic
CCAATATTATTACATAAACC+AGG 0.588965 6:+84228807 None:intergenic
TCAGCCGGTGAAAGTGGTGG+TGG 0.601771 6:-84229161 MsG0680034238.01.T01:CDS
CCCACCACCAGTGATACACC+TGG 0.602282 6:-84228825 MsG0680034238.01.T01:CDS
GTGAAAGTGGTGGTGGACTG+AGG 0.603007 6:-84229154 MsG0680034238.01.T01:CDS
AGTAGGTGGCTAGGGCAAAG+AGG 0.612636 6:+84229270 None:intergenic
GGTTTCATTGAACCATGGGT+AGG 0.618743 6:-84228939 MsG0680034238.01.T01:CDS
AAGTGGGTGGTAGTTAAAGG+TGG 0.629514 6:-84228960 MsG0680034238.01.T01:CDS
AAAGGATAATGGAAAGTAGG+TGG 0.636765 6:+84229256 None:intergenic
GGCAAAGAGGAAGAAATAGA+GGG 0.646580 6:+84229283 None:intergenic
ACTACAGATCTTCCGGATCG+AGG 0.646672 6:+84228693 None:intergenic
AAAGAGGAAGAAATAGAGGG+TGG 0.652446 6:+84229286 None:intergenic
CCACCACCAATACCTACCCA+TGG 0.653181 6:+84228927 None:intergenic
TGGTCAGCCGGTGAAAGTGG+TGG 0.679294 6:-84229164 MsG0680034238.01.T01:CDS
ATTTCTTACTCACAAGAACG+GGG 0.680212 6:+84229195 None:intergenic
TGCTGAATCCTCCAAGTGGG+TGG 0.681258 6:-84228973 MsG0680034238.01.T01:CDS
CCAGGTGTATCACTGGTGGT+GGG 0.686983 6:+84228825 None:intergenic
TAAACCAGGTGTATCACTGG+TGG 0.706773 6:+84228821 None:intergenic
ATGTGCTGAATCCTCCAAGT+GGG 0.714019 6:-84228976 MsG0680034238.01.T01:CDS
AATGTGCTGAATCCTCCAAG+TGG 0.759346 6:-84228977 MsG0680034238.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTAGATAATGAAAA+TGG - Chr6:84229211-84229230 MsG0680034238.01.T01:CDS 15.0%
!! TGAGTAAGAAATACTTTATT+TGG - Chr6:84228810-84228829 MsG0680034238.01.T01:CDS 20.0%
!!! AAATCATTGATATTTTCTGT+TGG + Chr6:84228947-84228966 None:intergenic 20.0%
!!! AATCATTGATATTTTCTGTT+GGG + Chr6:84228946-84228965 None:intergenic 20.0%
! ACAAATTGAGTAAAGGATAA+TGG + Chr6:84228752-84228771 None:intergenic 25.0%
! ATCAATGATTTCATCTTTAC+TGG - Chr6:84228956-84228975 MsG0680034238.01.T01:CDS 25.0%
! CCAATATTATTACATAAACC+AGG + Chr6:84229190-84229209 None:intergenic 25.0%
! CCTGGTTTATGTAATAATAT+TGG - Chr6:84229187-84229206 MsG0680034238.01.T01:CDS 25.0%
! TTGTTCAACAAATTGAGTAA+AGG + Chr6:84228759-84228778 None:intergenic 25.0%
!!! AATGGTAAGAGTATTATAGA+TGG - Chr6:84229094-84229113 MsG0680034238.01.T01:CDS 25.0%
ATCAACGAATACAACTTCAA+AGG + Chr6:84229269-84229288 None:intergenic 30.0%
GAACAAGTGAAGGATAAAAA+TGG - Chr6:84228773-84228792 MsG0680034238.01.T01:CDS 30.0%
GTATTTCTTACTCACAAGAA+CGG + Chr6:84228804-84228823 None:intergenic 30.0%
TATTTCTTACTCACAAGAAC+GGG + Chr6:84228803-84228822 None:intergenic 30.0%
TCAACGAATACAACTTCAAA+GGG + Chr6:84229268-84229287 None:intergenic 30.0%
! AAATACTTTATTTGGTCAGC+CGG - Chr6:84228818-84228837 MsG0680034238.01.T01:CDS 30.0%
! TTGTTTAAGATTGAGAGAAG+TGG - Chr6:84229118-84229137 MsG0680034238.01.T01:CDS 30.0%
!! AGTAAAGGATAATGGAAAGT+AGG + Chr6:84228744-84228763 None:intergenic 30.0%
!! TCAATTTGTTGAACAAGTGA+AGG - Chr6:84228763-84228782 MsG0680034238.01.T01:CDS 30.0%
!!! ATTGAGAGAAGTGGTTTTTT+TGG - Chr6:84229127-84229146 MsG0680034238.01.T01:CDS 30.0%
ATTTCTTACTCACAAGAACG+GGG + Chr6:84228802-84228821 None:intergenic 35.0%
TCTGTTGGGATAAATGTTAG+TGG + Chr6:84228932-84228951 None:intergenic 35.0%
!! AAAGGATAATGGAAAGTAGG+TGG + Chr6:84228741-84228760 None:intergenic 35.0%
!! TGTACAGCAAGCTTTTTCTA+AGG - Chr6:84228892-84228911 MsG0680034238.01.T01:CDS 35.0%
AAAGAGGAAGAAATAGAGGG+TGG + Chr6:84228711-84228730 None:intergenic 40.0%
AAGGTGGTTTCATTGAACCA+TGG - Chr6:84229050-84229069 MsG0680034238.01.T01:CDS 40.0%
ACATAAACCAGGTGTATCAC+TGG + Chr6:84229179-84229198 None:intergenic 40.0%
AGAGGGTGGTAAGTAATGTA+GGG + Chr6:84228697-84228716 None:intergenic 40.0%
AGGTGGTTTCATTGAACCAT+GGG - Chr6:84229051-84229070 MsG0680034238.01.T01:CDS 40.0%
ATTGAACCATGGGTAGGTAT+TGG - Chr6:84229061-84229080 MsG0680034238.01.T01:CDS 40.0%
GGCAAAGAGGAAGAAATAGA+GGG + Chr6:84228714-84228733 None:intergenic 40.0%
TAATGGAAAGTAGGTGGCTA+GGG + Chr6:84228735-84228754 None:intergenic 40.0%
TAGAGGGTGGTAAGTAATGT+AGG + Chr6:84228698-84228717 None:intergenic 40.0%
TTGGGATAAATGTTAGTGGC+AGG + Chr6:84228928-84228947 None:intergenic 40.0%
! ATAATGGAAAGTAGGTGGCT+AGG + Chr6:84228736-84228755 None:intergenic 40.0%
!! AAAGCTTGCTGTACAACAAG+TGG + Chr6:84228887-84228906 None:intergenic 40.0%
AAGTGGGTGGTAGTTAAAGG+TGG - Chr6:84229034-84229053 MsG0680034238.01.T01:CDS 45.0%
AATGTGCTGAATCCTCCAAG+TGG - Chr6:84229017-84229036 MsG0680034238.01.T01:CDS 45.0%
ACCTTTAACTACCACCCACT+TGG + Chr6:84229035-84229054 None:intergenic 45.0%
ATGTGCTGAATCCTCCAAGT+GGG - Chr6:84229018-84229037 MsG0680034238.01.T01:CDS 45.0%
GGGCAAAGAGGAAGAAATAG+AGG + Chr6:84228715-84228734 None:intergenic 45.0%
GGTTTCATTGAACCATGGGT+AGG - Chr6:84229055-84229074 MsG0680034238.01.T01:CDS 45.0%
TAAACCAGGTGTATCACTGG+TGG + Chr6:84229176-84229195 None:intergenic 45.0%
TCCAAGTGGGTGGTAGTTAA+AGG - Chr6:84229031-84229050 MsG0680034238.01.T01:CDS 45.0%
TTTAACTACCACCCACTTGG+AGG + Chr6:84229032-84229051 None:intergenic 45.0%
!! GGTATTGGTGGTGGTGTTAA+TGG - Chr6:84229076-84229095 MsG0680034238.01.T01:CDS 45.0%
ACTACAGATCTTCCGGATCG+AGG + Chr6:84229304-84229323 None:intergenic 50.0%
ATTTGGTCAGCCGGTGAAAG+TGG - Chr6:84228827-84228846 MsG0680034238.01.T01:CDS 50.0%
GTTGAAGATGCTCCTCGATC+CGG - Chr6:84229289-84229308 MsG0680034238.01.T01:CDS 50.0%
!! GAACCATGGGTAGGTATTGG+TGG - Chr6:84229064-84229083 MsG0680034238.01.T01:CDS 50.0%
ACCAGGTGTATCACTGGTGG+TGG + Chr6:84229173-84229192 None:intergenic 55.0%
AGTAGGTGGCTAGGGCAAAG+AGG + Chr6:84228727-84228746 None:intergenic 55.0%
CAGTCCACCACCACTTTCAC+CGG + Chr6:84228840-84228859 None:intergenic 55.0%
CCACCACCAATACCTACCCA+TGG + Chr6:84229070-84229089 None:intergenic 55.0%
CCAGGTGTATCACTGGTGGT+GGG + Chr6:84229172-84229191 None:intergenic 55.0%
TGCTGAATCCTCCAAGTGGG+TGG - Chr6:84229021-84229040 MsG0680034238.01.T01:CDS 55.0%
!! CCATGGGTAGGTATTGGTGG+TGG - Chr6:84229067-84229086 MsG0680034238.01.T01:CDS 55.0%
!! GTGAAAGTGGTGGTGGACTG+AGG - Chr6:84228840-84228859 MsG0680034238.01.T01:CDS 55.0%
CCCACCACCAGTGATACACC+TGG - Chr6:84229169-84229188 MsG0680034238.01.T01:CDS 60.0%
TCAGCCGGTGAAAGTGGTGG+TGG - Chr6:84228833-84228852 MsG0680034238.01.T01:CDS 60.0%
TGGTCAGCCGGTGAAAGTGG+TGG - Chr6:84228830-84228849 MsG0680034238.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr6 gene 84228689 84229327 84228689 ID=MsG0680034238.01;Name=MsG0680034238.01
Chr6 mRNA 84228689 84229327 84228689 ID=MsG0680034238.01.T01;Parent=MsG0680034238.01;Name=MsG0680034238.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|212
Chr6 exon 84228689 84229327 84228689 ID=MsG0680034238.01.T01:exon:11592;Parent=MsG0680034238.01.T01
Chr6 CDS 84228689 84229327 84228689 ID=MsG0680034238.01.T01:cds;Parent=MsG0680034238.01.T01
Gene Sequence

>MsG0680034238.01.T01

ATGAACCCTACATTACTTACCACCCTCTATTTCTTCCTCTTTGCCCTAGCCACCTACTTTCCATTATCCTTTACTCAATTTGTTGAACAAGTGAAGGATAAAAATGGAAACCCCGTTCTTGTGAGTAAGAAATACTTTATTTGGTCAGCCGGTGAAAGTGGTGGTGGACTGAGGCTTAATGAGACAGAAGAATGTCCACTTGTTGTACAGCAAGCTTTTTCTAAGGATGTTAAAAGCCTGCCACTAACATTTATCCCAACAGAAAATATCAATGATTTCATCTTTACTGGCTTCACATCACTAGATATTGTATTTGAAAAGAAGACAAAATGTGCTGAATCCTCCAAGTGGGTGGTAGTTAAAGGTGGTTTCATTGAACCATGGGTAGGTATTGGTGGTGGTGTTAATGGTAAGAGTATTATAGATGGTTTGTTTAAGATTGAGAGAAGTGGTTTTTTTGGATACAAGCTTGTGTTTTGTCCCACCACCAGTGATACACCTGGTTTATGTAATAATATTGGAAGATTTTTAGATAATGAAAATGGAGTGCGTTTGATCATGAGTGAAAATTTTCAACCCTTTGAAGTTGTATTCGTTGATGTTGAAGATGCTCCTCGATCCGGAAGATCTGTAGTTTGA

Protein sequence

>MsG0680034238.01.T01

MNPTLLTTLYFFLFALATYFPLSFTQFVEQVKDKNGNPVLVSKKYFIWSAGESGGGLRLNETEECPLVVQQAFSKDVKSLPLTFIPTENINDFIFTGFTSLDIVFEKKTKCAESSKWVVVKGGFIEPWVGIGGGVNGKSIIDGLFKIERSGFFGYKLVFCPTTSDTPGLCNNIGRFLDNENGVRLIMSENFQPFEVVFVDVEDAPRSGRSVV*