Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009767.01.T01 | KEH38386.1 | 96.38 | 221 | 7 | 1 | 1 | 220 | 1 | 221 | 2.94E-153 | 434 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009767.01.T01 | P49332 | 67.123 | 219 | 70 | 2 | 3 | 220 | 4 | 221 | 5.13E-105 | 305 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009767.01.T01 | A0A072V9X8 | 96.380 | 221 | 7 | 1 | 1 | 220 | 1 | 221 | 1.41e-153 | 434 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048509.01 | MsG0280009767.01 | 0.802630 | 5.120270e-49 | 2.520463e-46 |
| MsG0180003375.01 | MsG0280009767.01 | 0.811772 | 5.922857e-51 | 3.698756e-48 |
| MsG0180005417.01 | MsG0280009767.01 | 0.801146 | 1.033096e-48 | 4.897632e-46 |
| MsG0180005572.01 | MsG0280009767.01 | 0.822802 | 1.949240e-53 | 1.648478e-50 |
| MsG0180005757.01 | MsG0280009767.01 | 0.817210 | 3.711049e-52 | 2.685759e-49 |
| MsG0280009526.01 | MsG0280009767.01 | 0.813071 | 3.081360e-51 | 1.992176e-48 |
| MsG0280009767.01 | MsG0280011267.01 | 0.812380 | 4.365719e-51 | 2.771492e-48 |
| MsG0280009767.01 | MsG0380011974.01 | 0.802505 | 5.434581e-49 | 2.666676e-46 |
| MsG0280009767.01 | MsG0380014639.01 | -0.811861 | 5.663811e-51 | 3.545335e-48 |
| MsG0280009767.01 | MsG0380015323.01 | 0.801964 | 7.022852e-49 | 3.398667e-46 |
| MsG0280009767.01 | MsG0380018004.01 | 0.824247 | 8.951863e-54 | 7.885594e-51 |
| MsG0280009767.01 | MsG0480021396.01 | 0.805944 | 1.045021e-49 | 5.601276e-47 |
| MsG0280009767.01 | MsG0480022428.01 | 0.802737 | 4.866962e-49 | 2.402394e-46 |
| MsG0280009767.01 | MsG0480023322.01 | 0.814265 | 1.682144e-51 | 1.123437e-48 |
| MsG0280009767.01 | MsG0580026311.01 | 0.805155 | 1.529988e-49 | 8.035964e-47 |
| MsG0280009767.01 | MsG0680030613.01 | 0.812151 | 4.897511e-51 | 3.089990e-48 |
| MsG0280009767.01 | MsG0680030960.01 | 0.811206 | 7.860792e-51 | 4.836288e-48 |
| MsG0280009767.01 | MsG0680033367.01 | 0.801533 | 8.607919e-49 | 4.120744e-46 |
| MsG0280009767.01 | MsG0680034418.01 | 0.806419 | 8.302173e-50 | 4.503937e-47 |
| MsG0280009767.01 | MsG0780036480.01 | 0.807891 | 4.049945e-50 | 2.282423e-47 |
| MsG0280009767.01 | MsG0780037182.01 | 0.812545 | 4.018230e-51 | 2.562160e-48 |
| MsG0280009767.01 | MsG0780039619.01 | 0.816891 | 4.376570e-52 | 3.139850e-49 |
| MsG0280009767.01 | MsG0780039916.01 | 0.807772 | 4.293105e-50 | 2.412054e-47 |
| MsG0280009767.01 | MsG0780039979.01 | 0.815829 | 7.566544e-52 | 5.272715e-49 |
| MsG0280009767.01 | MsG0780041180.01 | 0.819069 | 1.409684e-52 | 1.073729e-49 |
| MsG0280009767.01 | MsG0880043550.01 | 0.817278 | 3.582399e-52 | 2.597356e-49 |
| MsG0280009767.01 | MsG0880043953.01 | 0.809900 | 1.505374e-50 | 8.947293e-48 |
| MsG0280009767.01 | MsG0880046071.01 | 0.810383 | 1.184710e-50 | 7.130541e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009767.01.T01 | MTR_2g070200 | 96.380 | 221 | 7 | 1 | 1 | 220 | 1 | 221 | 3.56e-157 | 434 |
| MsG0280009767.01.T01 | MTR_2g070150 | 74.146 | 205 | 52 | 1 | 1 | 205 | 1 | 204 | 3.02e-117 | 332 |
| MsG0280009767.01.T01 | MTR_2g070210 | 69.820 | 222 | 64 | 2 | 1 | 220 | 1 | 221 | 1.97e-114 | 326 |
| MsG0280009767.01.T01 | MTR_2g070180 | 68.349 | 218 | 67 | 2 | 1 | 218 | 1 | 216 | 4.01e-113 | 322 |
| MsG0280009767.01.T01 | MTR_2g070140 | 71.569 | 204 | 57 | 1 | 17 | 220 | 1 | 203 | 2.57e-111 | 317 |
| MsG0280009767.01.T01 | MTR_2g070130 | 64.091 | 220 | 78 | 1 | 1 | 220 | 1 | 219 | 6.44e-108 | 309 |
| MsG0280009767.01.T01 | MTR_2g070060 | 59.361 | 219 | 88 | 1 | 1 | 219 | 1 | 218 | 2.97e-97 | 282 |
| MsG0280009767.01.T01 | MTR_2g070070 | 61.187 | 219 | 84 | 1 | 1 | 219 | 1 | 218 | 8.78e-97 | 281 |
| MsG0280009767.01.T01 | MTR_3g467420 | 62.037 | 216 | 81 | 1 | 5 | 220 | 10 | 224 | 3.58e-96 | 280 |
| MsG0280009767.01.T01 | MTR_3g467430 | 63.285 | 207 | 75 | 1 | 3 | 209 | 8 | 213 | 5.37e-92 | 269 |
| MsG0280009767.01.T01 | MTR_8g056940 | 59.447 | 217 | 87 | 1 | 1 | 217 | 1 | 216 | 8.85e-92 | 268 |
| MsG0280009767.01.T01 | MTR_4g124130 | 57.727 | 220 | 91 | 2 | 1 | 219 | 1 | 219 | 8.94e-90 | 263 |
| MsG0280009767.01.T01 | MTR_2g070110 | 57.078 | 219 | 92 | 1 | 1 | 219 | 1 | 217 | 5.08e-89 | 261 |
| MsG0280009767.01.T01 | MTR_2g070120 | 59.524 | 210 | 84 | 1 | 1 | 210 | 1 | 209 | 7.01e-88 | 258 |
| MsG0280009767.01.T01 | MTR_5g076900 | 47.727 | 220 | 112 | 3 | 2 | 220 | 4 | 221 | 6.95e-68 | 208 |
| MsG0280009767.01.T01 | MTR_0186s0030 | 60.563 | 142 | 55 | 1 | 1 | 142 | 1 | 141 | 6.56e-57 | 177 |
| MsG0280009767.01.T01 | MTR_1g090060 | 42.202 | 218 | 120 | 4 | 4 | 217 | 3 | 218 | 1.80e-52 | 169 |
| MsG0280009767.01.T01 | MTR_4g059730 | 42.081 | 221 | 120 | 5 | 1 | 216 | 1 | 218 | 1.58e-51 | 166 |
| MsG0280009767.01.T01 | MTR_1g090070 | 39.726 | 219 | 125 | 5 | 4 | 217 | 9 | 225 | 7.07e-50 | 162 |
| MsG0280009767.01.T01 | MTR_8g087425 | 41.546 | 207 | 117 | 3 | 2 | 206 | 3 | 207 | 1.21e-49 | 161 |
| MsG0280009767.01.T01 | MTR_7g065720 | 40.952 | 210 | 119 | 4 | 3 | 209 | 6 | 213 | 1.26e-48 | 159 |
| MsG0280009767.01.T01 | MTR_5g040430 | 36.792 | 212 | 126 | 3 | 2 | 206 | 5 | 215 | 8.32e-48 | 157 |
| MsG0280009767.01.T01 | MTR_5g037380 | 40.278 | 216 | 121 | 3 | 2 | 209 | 3 | 218 | 1.05e-47 | 157 |
| MsG0280009767.01.T01 | MTR_7g065740 | 40.758 | 211 | 118 | 5 | 3 | 209 | 6 | 213 | 1.12e-46 | 154 |
| MsG0280009767.01.T01 | MTR_7g065660 | 40.476 | 210 | 121 | 3 | 3 | 210 | 6 | 213 | 2.54e-46 | 153 |
| MsG0280009767.01.T01 | MTR_1g090100 | 42.105 | 209 | 115 | 4 | 5 | 209 | 3 | 209 | 3.73e-46 | 152 |
| MsG0280009767.01.T01 | MTR_3g099757 | 42.308 | 208 | 116 | 4 | 3 | 208 | 4 | 209 | 5.40e-46 | 152 |
| MsG0280009767.01.T01 | MTR_8g087410 | 38.605 | 215 | 128 | 3 | 4 | 216 | 6 | 218 | 7.25e-45 | 149 |
| MsG0280009767.01.T01 | MTR_4g019780 | 39.171 | 217 | 126 | 4 | 3 | 216 | 7 | 220 | 8.15e-45 | 149 |
| MsG0280009767.01.T01 | MTR_1g090090 | 41.284 | 218 | 122 | 5 | 4 | 217 | 3 | 218 | 3.40e-44 | 147 |
| MsG0280009767.01.T01 | MTR_7g065590 | 39.524 | 210 | 123 | 3 | 3 | 210 | 6 | 213 | 1.22e-43 | 146 |
| MsG0280009767.01.T01 | MTR_7g065600 | 40.796 | 201 | 115 | 3 | 3 | 201 | 6 | 204 | 1.34e-43 | 146 |
| MsG0280009767.01.T01 | MTR_7g065750 | 40.594 | 202 | 115 | 4 | 3 | 201 | 6 | 205 | 1.50e-43 | 146 |
| MsG0280009767.01.T01 | MTR_1g115195 | 41.706 | 211 | 115 | 6 | 1 | 206 | 1 | 208 | 2.29e-43 | 145 |
| MsG0280009767.01.T01 | MTR_4g019790 | 40.000 | 200 | 116 | 3 | 4 | 201 | 7 | 204 | 4.03e-43 | 145 |
| MsG0280009767.01.T01 | MTR_1g090150 | 38.532 | 218 | 125 | 6 | 3 | 215 | 2 | 215 | 2.18e-42 | 143 |
| MsG0280009767.01.T01 | MTR_7g065265 | 41.627 | 209 | 118 | 4 | 3 | 209 | 6 | 212 | 2.91e-42 | 142 |
| MsG0280009767.01.T01 | MTR_6g080440 | 41.919 | 198 | 107 | 5 | 18 | 211 | 17 | 210 | 3.92e-42 | 142 |
| MsG0280009767.01.T01 | MTR_7g065270 | 41.148 | 209 | 119 | 4 | 3 | 209 | 6 | 212 | 4.74e-42 | 142 |
| MsG0280009767.01.T01 | MTR_7g065230 | 40.807 | 223 | 113 | 5 | 3 | 218 | 6 | 216 | 7.38e-42 | 141 |
| MsG0280009767.01.T01 | MTR_7g065290 | 41.148 | 209 | 119 | 4 | 3 | 209 | 6 | 212 | 6.51e-41 | 139 |
| MsG0280009767.01.T01 | MTR_7g065710 | 38.647 | 207 | 123 | 4 | 1 | 207 | 1 | 203 | 6.90e-41 | 139 |
| MsG0280009767.01.T01 | MTR_7g065630 | 39.423 | 208 | 120 | 6 | 3 | 209 | 24 | 226 | 1.02e-40 | 139 |
| MsG0280009767.01.T01 | MTR_8g061950 | 38.498 | 213 | 121 | 4 | 3 | 206 | 4 | 215 | 3.48e-40 | 137 |
| MsG0280009767.01.T01 | MTR_7g065640 | 38.462 | 208 | 108 | 5 | 3 | 209 | 6 | 194 | 2.23e-38 | 132 |
| MsG0280009767.01.T01 | MTR_7g065260 | 41.294 | 201 | 114 | 3 | 3 | 201 | 13 | 211 | 1.45e-36 | 128 |
| MsG0280009767.01.T01 | MTR_7g065700 | 37.799 | 209 | 126 | 4 | 1 | 209 | 4 | 208 | 3.25e-36 | 127 |
| MsG0280009767.01.T01 | MTR_7g065680 | 38.647 | 207 | 119 | 5 | 3 | 207 | 6 | 206 | 4.53e-36 | 127 |
| MsG0280009767.01.T01 | MTR_2g072120 | 60.274 | 73 | 29 | 0 | 47 | 119 | 1 | 73 | 1.47e-29 | 105 |
| MsG0280009767.01.T01 | MTR_4g134380 | 30.588 | 170 | 102 | 4 | 4 | 160 | 15 | 181 | 8.22e-13 | 65.5 |
| MsG0280009767.01.T01 | MTR_4g043640 | 57.143 | 42 | 18 | 0 | 149 | 190 | 44 | 85 | 3.75e-12 | 60.5 |
| MsG0280009767.01.T01 | MTR_7g100320 | 29.851 | 201 | 127 | 9 | 11 | 206 | 33 | 224 | 1.33e-11 | 62.4 |
| MsG0280009767.01.T01 | MTR_4g134370 | 26.887 | 212 | 135 | 7 | 3 | 201 | 14 | 218 | 1.51e-11 | 62.0 |
| MsG0280009767.01.T01 | MTR_1g067180 | 29.897 | 194 | 116 | 9 | 14 | 199 | 37 | 218 | 2.06e-11 | 62.0 |
| MsG0280009767.01.T01 | MTR_1g067180 | 29.442 | 197 | 119 | 9 | 11 | 199 | 31 | 215 | 2.57e-11 | 61.6 |
| MsG0280009767.01.T01 | MTR_4g134380 | 29.878 | 164 | 99 | 4 | 4 | 154 | 15 | 175 | 4.05e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280009767.01.T01 | AT1G78380 | 64.055 | 217 | 77 | 1 | 1 | 217 | 1 | 216 | 1.67e-102 | 296 |
| MsG0280009767.01.T01 | AT1G17180 | 62.273 | 220 | 82 | 1 | 1 | 220 | 1 | 219 | 4.19e-102 | 295 |
| MsG0280009767.01.T01 | AT1G78340 | 63.158 | 209 | 76 | 1 | 1 | 209 | 1 | 208 | 2.74e-98 | 285 |
| MsG0280009767.01.T01 | AT1G17170 | 61.364 | 220 | 83 | 2 | 1 | 220 | 1 | 218 | 6.76e-97 | 281 |
| MsG0280009767.01.T01 | AT1G78370 | 60.930 | 215 | 83 | 1 | 1 | 215 | 1 | 214 | 7.50e-96 | 279 |
| MsG0280009767.01.T01 | AT1G78360 | 61.086 | 221 | 84 | 2 | 1 | 220 | 1 | 220 | 5.51e-95 | 276 |
| MsG0280009767.01.T01 | AT1G78320 | 59.070 | 215 | 87 | 1 | 1 | 215 | 1 | 214 | 1.29e-92 | 271 |
| MsG0280009767.01.T01 | AT1G53680 | 56.221 | 217 | 94 | 1 | 5 | 220 | 8 | 224 | 6.30e-89 | 261 |
| MsG0280009767.01.T01 | AT1G17190 | 54.167 | 216 | 98 | 1 | 3 | 218 | 4 | 218 | 5.15e-84 | 249 |
| MsG0280009767.01.T01 | AT3G43800 | 51.152 | 217 | 101 | 4 | 3 | 215 | 4 | 219 | 9.32e-77 | 231 |
| MsG0280009767.01.T01 | AT1G78320 | 50.698 | 215 | 78 | 2 | 1 | 215 | 1 | 187 | 6.94e-71 | 214 |
| MsG0280009767.01.T01 | AT2G29420 | 44.796 | 221 | 118 | 4 | 2 | 220 | 7 | 225 | 1.32e-57 | 182 |
| MsG0280009767.01.T01 | AT3G09270 | 42.512 | 207 | 115 | 3 | 3 | 206 | 5 | 210 | 2.75e-50 | 163 |
| MsG0280009767.01.T01 | AT1G10360 | 42.326 | 215 | 117 | 3 | 2 | 209 | 3 | 217 | 6.07e-50 | 162 |
| MsG0280009767.01.T01 | AT2G29490 | 39.810 | 211 | 118 | 6 | 3 | 208 | 6 | 212 | 3.73e-47 | 155 |
| MsG0280009767.01.T01 | AT1G59700 | 41.743 | 218 | 119 | 4 | 3 | 212 | 5 | 222 | 2.62e-46 | 153 |
| MsG0280009767.01.T01 | AT1G10370 | 40.930 | 215 | 120 | 3 | 2 | 209 | 3 | 217 | 8.76e-46 | 152 |
| MsG0280009767.01.T01 | AT2G29480 | 39.810 | 211 | 118 | 6 | 3 | 208 | 6 | 212 | 3.68e-45 | 150 |
| MsG0280009767.01.T01 | AT2G29440 | 38.636 | 220 | 126 | 6 | 3 | 217 | 5 | 220 | 6.51e-45 | 149 |
| MsG0280009767.01.T01 | AT1G74590 | 37.981 | 208 | 121 | 4 | 3 | 204 | 6 | 211 | 5.14e-44 | 147 |
| MsG0280009767.01.T01 | AT2G29450 | 38.208 | 212 | 125 | 4 | 3 | 210 | 5 | 214 | 2.27e-43 | 145 |
| MsG0280009767.01.T01 | AT1G27130 | 37.674 | 215 | 126 | 3 | 3 | 209 | 5 | 219 | 2.46e-43 | 145 |
| MsG0280009767.01.T01 | AT2G29460 | 37.727 | 220 | 128 | 6 | 3 | 217 | 6 | 221 | 2.50e-43 | 145 |
| MsG0280009767.01.T01 | AT5G62480 | 36.937 | 222 | 125 | 5 | 1 | 210 | 5 | 223 | 1.53e-41 | 141 |
| MsG0280009767.01.T01 | AT1G69930 | 38.389 | 211 | 124 | 3 | 5 | 209 | 13 | 223 | 1.72e-41 | 141 |
| MsG0280009767.01.T01 | AT2G29470 | 36.986 | 219 | 128 | 7 | 5 | 217 | 8 | 222 | 1.90e-41 | 140 |
| MsG0280009767.01.T01 | AT1G59670 | 39.450 | 218 | 124 | 4 | 3 | 212 | 5 | 222 | 2.34e-41 | 140 |
| MsG0280009767.01.T01 | AT1G27140 | 36.866 | 217 | 128 | 3 | 3 | 210 | 5 | 221 | 3.67e-41 | 140 |
| MsG0280009767.01.T01 | AT1G69920 | 34.081 | 223 | 136 | 4 | 5 | 219 | 35 | 254 | 5.76e-36 | 127 |
| MsG0280009767.01.T01 | AT5G62480 | 29.730 | 222 | 115 | 6 | 1 | 210 | 5 | 197 | 1.08e-23 | 94.7 |
| MsG0280009767.01.T01 | AT5G62480 | 29.730 | 222 | 114 | 6 | 1 | 210 | 5 | 196 | 1.37e-23 | 94.4 |
| MsG0280009767.01.T01 | AT5G02780 | 27.941 | 204 | 134 | 8 | 11 | 209 | 37 | 232 | 2.22e-11 | 62.0 |
| MsG0280009767.01.T01 | AT5G02780 | 27.941 | 204 | 134 | 8 | 11 | 209 | 37 | 232 | 2.22e-11 | 62.0 |
| MsG0280009767.01.T01 | AT5G02780 | 28.866 | 194 | 123 | 8 | 11 | 199 | 37 | 220 | 3.63e-11 | 61.2 |
| MsG0280009767.01.T01 | AT5G02790 | 31.472 | 197 | 109 | 10 | 11 | 199 | 35 | 213 | 3.93e-11 | 61.2 |
Find 57 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGGGAGACAAGGCTTATTT+TGG | 0.162898 | 2:-57720828 | MsG0280009767.01.T01:CDS |
| TGAGAGCACAAGCTAGATTT+TGG | 0.184304 | 2:-57721623 | MsG0280009767.01.T01:CDS |
| ACTTAATGCCTAATTTCTTT+AGG | 0.190935 | 2:+57720607 | None:intergenic |
| CTTAATGCCTAATTTCTTTA+GGG | 0.237574 | 2:+57720608 | None:intergenic |
| CATATCTATGAATTCCTTCT+TGG | 0.269924 | 2:+57720872 | None:intergenic |
| TTTGAAACATATGAGAAGTT+TGG | 0.284132 | 2:-57720753 | MsG0280009767.01.T01:CDS |
| ATTACAAGTGACTCACAAAT+AGG | 0.297067 | 2:+57721708 | None:intergenic |
| ATAAAGATGCAAGGGATAAT+TGG | 0.310757 | 2:-57721794 | MsG0280009767.01.T01:CDS |
| GGTTTCTGGATAAGTCCTTA+TGG | 0.328976 | 2:-57721870 | MsG0280009767.01.T01:CDS |
| CATAGATATGCTCAAATTGT+TGG | 0.364566 | 2:-57720859 | MsG0280009767.01.T01:CDS |
| AAGCTGGAAGGAAAGTTTAT+AGG | 0.375912 | 2:-57720923 | MsG0280009767.01.T01:CDS |
| GTGCCCTAAGTTCATTGCTT+GGG | 0.394706 | 2:-57720709 | MsG0280009767.01.T01:CDS |
| AAGAATAGCCCTTGCTGAAA+AGG | 0.396907 | 2:-57721838 | MsG0280009767.01.T01:CDS |
| GCAGATGCCCTAAAGAAATT+AGG | 0.402688 | 2:-57720615 | MsG0280009767.01.T01:CDS |
| TTGCCATTGTGGATTAGAAC+CGG | 0.405758 | 2:+57721732 | None:intergenic |
| CACAAATAGGTTTGCCATTG+TGG | 0.406278 | 2:+57721721 | None:intergenic |
| TCGTAGACCTTATGCTGGTT+AGG | 0.419263 | 2:+57720645 | None:intergenic |
| TTGAGTATATTGATGAGGTT+TGG | 0.428745 | 2:-57721683 | MsG0280009767.01.T01:CDS |
| AGTGCCCTAAGTTCATTGCT+TGG | 0.433594 | 2:-57720710 | MsG0280009767.01.T01:CDS |
| TTAGCCCAAGCAATGAACTT+AGG | 0.435179 | 2:+57720705 | None:intergenic |
| AAAATCTAGCTTGTGCTCTC+AGG | 0.436530 | 2:+57721625 | None:intergenic |
| TGGAGGAGACAGACTTGGTT+TGG | 0.440850 | 2:-57720808 | MsG0280009767.01.T01:CDS |
| GGCACTCAATCTCTATATTG+AGG | 0.444351 | 2:+57720727 | None:intergenic |
| GTTTCAAACCAACTGTAGAA+TGG | 0.455904 | 2:+57720768 | None:intergenic |
| TTTCAAACCAACTGTAGAAT+GGG | 0.460044 | 2:+57720769 | None:intergenic |
| ATTCTTGCTCTCATTCCATA+AGG | 0.463260 | 2:+57721855 | None:intergenic |
| GGCTGACTATGTTGATAAAA+CGG | 0.464640 | 2:-57721601 | MsG0280009767.01.T01:intron |
| CAAATTGTTGGAGAAACAGC+TGG | 0.465628 | 2:-57720847 | MsG0280009767.01.T01:CDS |
| TAGCCCAAGCAATGAACTTA+GGG | 0.471249 | 2:+57720706 | None:intergenic |
| CCCTAGTCCCATTCTACAGT+TGG | 0.473896 | 2:-57720776 | MsG0280009767.01.T01:CDS |
| GTCTCTTCCTAACCAGCATA+AGG | 0.491160 | 2:-57720652 | MsG0280009767.01.T01:CDS |
| AGTTCATTGCTTGGGCTAAG+AGG | 0.495968 | 2:-57720701 | MsG0280009767.01.T01:CDS |
| AGAATAGCCCTTGCTGAAAA+GGG | 0.508196 | 2:-57721837 | MsG0280009767.01.T01:CDS |
| GGGTTCATTTGTAGAAGATA+AGG | 0.510488 | 2:+57721771 | None:intergenic |
| AGAACAAGAAGCTGCCAAGA+AGG | 0.534444 | 2:-57720886 | MsG0280009767.01.T01:CDS |
| CCAATTCATGGCTGATGAGG+TGG | 0.538382 | 2:-57721901 | None:intergenic |
| TGTCAATAGATATATGAAGC+TGG | 0.543339 | 2:-57720939 | MsG0280009767.01.T01:intron |
| GTATATTGATGAGGTTTGGA+AGG | 0.548215 | 2:-57721679 | MsG0280009767.01.T01:CDS |
| TGCCATTGTGGATTAGAACC+GGG | 0.551246 | 2:+57721733 | None:intergenic |
| CTAGCTTGTGCTCTCAGGTA+GGG | 0.551544 | 2:+57721630 | None:intergenic |
| TCTAGCTTGTGCTCTCAGGT+AGG | 0.573698 | 2:+57721629 | None:intergenic |
| CTACCAATTCATGGCTGATG+AGG | 0.581215 | 2:-57721904 | None:intergenic |
| CCCGGTTCACAAGAAAATCC+CGG | 0.589293 | 2:-57721751 | MsG0280009767.01.T01:CDS |
| GGAGAAACAGCTGGGAGACA+AGG | 0.589733 | 2:-57720838 | MsG0280009767.01.T01:CDS |
| AATAGATATATGAAGCTGGA+AGG | 0.589985 | 2:-57720935 | MsG0280009767.01.T01:intron |
| CCAACTGTAGAATGGGACTA+GGG | 0.601552 | 2:+57720776 | None:intergenic |
| GTTCATAGATAAAGATGCAA+GGG | 0.604079 | 2:-57721802 | MsG0280009767.01.T01:CDS |
| ATGCTGGTTAGGAAGAGACT+TGG | 0.605348 | 2:+57720656 | None:intergenic |
| GCTCTCAGGTAGGGATCAGA+AGG | 0.607625 | 2:+57721639 | None:intergenic |
| ACCAACTGTAGAATGGGACT+AGG | 0.615269 | 2:+57720775 | None:intergenic |
| AGTTCATAGATAAAGATGCA+AGG | 0.625022 | 2:-57721803 | MsG0280009767.01.T01:CDS |
| TGAACTCGTAGACCTTATGC+TGG | 0.626524 | 2:+57720640 | None:intergenic |
| AAATTGTTGGAGAAACAGCT+GGG | 0.647590 | 2:-57720846 | MsG0280009767.01.T01:CDS |
| TTATCTTCTACAAATGAACC+CGG | 0.663975 | 2:-57721769 | MsG0280009767.01.T01:CDS |
| TAGGGATCAGAAGGCAACAA+CGG | 0.667696 | 2:+57721648 | None:intergenic |
| AATTGTTGAGTATATTGATG+AGG | 0.670411 | 2:-57721688 | MsG0280009767.01.T01:CDS |
| ATCCCGGTTCTAATCCACAA+TGG | 0.688019 | 2:-57721735 | MsG0280009767.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAACAAATAATAAATAT+TGG | + | Chr2:57721166-57721185 | None:intergenic | 10.0% |
| !!! | TTCTTTAATTTTTTTTAAAG+AGG | - | Chr2:57721181-57721200 | MsG0280009767.01.T01:intron | 10.0% |
| !! | AACAAATAATAAATATTGGA+TGG | + | Chr2:57721162-57721181 | None:intergenic | 15.0% |
| !! | GGATTAAAAATTTATTGAAT+GGG | - | Chr2:57721332-57721351 | MsG0280009767.01.T01:intron | 15.0% |
| !! | TGGATTAAAAATTTATTGAA+TGG | - | Chr2:57721331-57721350 | MsG0280009767.01.T01:intron | 15.0% |
| !!! | TTAATTTTTTTTAAAGAGGA+AGG | - | Chr2:57721185-57721204 | MsG0280009767.01.T01:intron | 15.0% |
| !! | AATGTAATTTGATATTGAGT+AGG | + | Chr2:57721369-57721388 | None:intergenic | 20.0% |
| !! | TACTCAATATCAAATTACAT+TGG | - | Chr2:57721368-57721387 | MsG0280009767.01.T01:intron | 20.0% |
| ! | AAAGTTTATAGGACCAAAAA+AGG | - | Chr2:57721587-57721606 | MsG0280009767.01.T01:intron | 25.0% |
| ! | AATTAACACCCAAAATTAGT+TGG | + | Chr2:57721535-57721554 | None:intergenic | 25.0% |
| ! | AATTGTTGAGTATATTGATG+AGG | - | Chr2:57720811-57720830 | MsG0280009767.01.T01:CDS | 25.0% |
| ! | ACTTAATGCCTAATTTCTTT+AGG | + | Chr2:57721895-57721914 | None:intergenic | 25.0% |
| ! | CTTAATGCCTAATTTCTTTA+GGG | + | Chr2:57721894-57721913 | None:intergenic | 25.0% |
| ! | GATATTTAATCAGTTGAGTT+AGG | - | Chr2:57721060-57721079 | MsG0280009767.01.T01:intron | 25.0% |
| ! | TGTAGGCAAAAATAAGTTAT+TGG | + | Chr2:57721291-57721310 | None:intergenic | 25.0% |
| ! | TTTATTGAATGGGTCTAATA+TGG | - | Chr2:57721342-57721361 | MsG0280009767.01.T01:intron | 25.0% |
| !! | ACGAATTTTGTAAAATGTGT+AGG | + | Chr2:57721308-57721327 | None:intergenic | 25.0% |
| !! | TTTGAAACATATGAGAAGTT+TGG | - | Chr2:57721746-57721765 | MsG0280009767.01.T01:CDS | 25.0% |
| !!! | TGTTGTTTCCAACTAATTTT+GGG | - | Chr2:57721524-57721543 | MsG0280009767.01.T01:intron | 25.0% |
| AATAGATATATGAAGCTGGA+AGG | - | Chr2:57721564-57721583 | MsG0280009767.01.T01:intron | 30.0% | |
| AGTTCATAGATAAAGATGCA+AGG | - | Chr2:57720696-57720715 | MsG0280009767.01.T01:CDS | 30.0% | |
| ATAAAGATGCAAGGGATAAT+TGG | - | Chr2:57720705-57720724 | MsG0280009767.01.T01:CDS | 30.0% | |
| ATTACAAGTGACTCACAAAT+AGG | + | Chr2:57720794-57720813 | None:intergenic | 30.0% | |
| CATAGATATGCTCAAATTGT+TGG | - | Chr2:57721640-57721659 | MsG0280009767.01.T01:CDS | 30.0% | |
| CATATCTATGAATTCCTTCT+TGG | + | Chr2:57721630-57721649 | None:intergenic | 30.0% | |
| CCATTTCACTAAAAGTAGTA+AGG | - | Chr2:57721461-57721480 | MsG0280009767.01.T01:intron | 30.0% | |
| GAGAAGACATCAAATTACAT+TGG | - | Chr2:57721242-57721261 | MsG0280009767.01.T01:intron | 30.0% | |
| GTTCATAGATAAAGATGCAA+GGG | - | Chr2:57720697-57720716 | MsG0280009767.01.T01:CDS | 30.0% | |
| TGTCAATAGATATATGAAGC+TGG | - | Chr2:57721560-57721579 | MsG0280009767.01.T01:intron | 30.0% | |
| TTATCTTCTACAAATGAACC+CGG | - | Chr2:57720730-57720749 | MsG0280009767.01.T01:CDS | 30.0% | |
| TTGAGTATATTGATGAGGTT+TGG | - | Chr2:57720816-57720835 | MsG0280009767.01.T01:CDS | 30.0% | |
| TTTCAAACCAACTGTAGAAT+GGG | + | Chr2:57721733-57721752 | None:intergenic | 30.0% | |
| ! | CATTTTACAAAATTCGTCGT+TGG | - | Chr2:57721311-57721330 | MsG0280009767.01.T01:intron | 30.0% |
| ! | CTTTCATCGATCTCTATTTT+AGG | - | Chr2:57721124-57721143 | MsG0280009767.01.T01:intron | 30.0% |
| !! | CCTTACTACTTTTAGTGAAA+TGG | + | Chr2:57721464-57721483 | None:intergenic | 30.0% |
| !!! | GTGTTGTTTCCAACTAATTT+TGG | - | Chr2:57721523-57721542 | MsG0280009767.01.T01:intron | 30.0% |
| !!! | TCGATCTCTATTTTAGGTTT+TGG | - | Chr2:57721130-57721149 | MsG0280009767.01.T01:intron | 30.0% |
| !!! | TTCTTGTTCTTCTCCTTTTT+TGG | + | Chr2:57721603-57721622 | None:intergenic | 30.0% |
| AAATTGTTGGAGAAACAGCT+GGG | - | Chr2:57721653-57721672 | MsG0280009767.01.T01:CDS | 35.0% | |
| AAGCTGGAAGGAAAGTTTAT+AGG | - | Chr2:57721576-57721595 | MsG0280009767.01.T01:intron | 35.0% | |
| ATTCTTGCTCTCATTCCATA+AGG | + | Chr2:57720647-57720666 | None:intergenic | 35.0% | |
| GGGTTCATTTGTAGAAGATA+AGG | + | Chr2:57720731-57720750 | None:intergenic | 35.0% | |
| GTATATTGATGAGGTTTGGA+AGG | - | Chr2:57720820-57720839 | MsG0280009767.01.T01:CDS | 35.0% | |
| GTTTCAAACCAACTGTAGAA+TGG | + | Chr2:57721734-57721753 | None:intergenic | 35.0% | |
| TTGAAACTCACATACACAAG+TGG | - | Chr2:57721000-57721019 | MsG0280009767.01.T01:intron | 35.0% | |
| ! | GGCTGACTATGTTGATAAAA+CGG | - | Chr2:57720898-57720917 | MsG0280009767.01.T01:CDS | 35.0% |
| !!! | CTCTATTTTAGGTTTTGGTG+TGG | - | Chr2:57721135-57721154 | MsG0280009767.01.T01:intron | 35.0% |
| AACTCACATACACAAGTGGA+AGG | - | Chr2:57721004-57721023 | MsG0280009767.01.T01:intron | 40.0% | |
| AAGAATAGCCCTTGCTGAAA+AGG | - | Chr2:57720661-57720680 | MsG0280009767.01.T01:CDS | 40.0% | |
| AGAATAGCCCTTGCTGAAAA+GGG | - | Chr2:57720662-57720681 | MsG0280009767.01.T01:CDS | 40.0% | |
| CAAATTGTTGGAGAAACAGC+TGG | - | Chr2:57721652-57721671 | MsG0280009767.01.T01:CDS | 40.0% | |
| CACAAATAGGTTTGCCATTG+TGG | + | Chr2:57720781-57720800 | None:intergenic | 40.0% | |
| GCAGATGCCCTAAAGAAATT+AGG | - | Chr2:57721884-57721903 | MsG0280009767.01.T01:CDS | 40.0% | |
| GGCACTCAATCTCTATATTG+AGG | + | Chr2:57721775-57721794 | None:intergenic | 40.0% | |
| TAGCCCAAGCAATGAACTTA+GGG | + | Chr2:57721796-57721815 | None:intergenic | 40.0% | |
| TTAGCCCAAGCAATGAACTT+AGG | + | Chr2:57721797-57721816 | None:intergenic | 40.0% | |
| ! | AAAATCTAGCTTGTGCTCTC+AGG | + | Chr2:57720877-57720896 | None:intergenic | 40.0% |
| ! | GGTTTCTGGATAAGTCCTTA+TGG | - | Chr2:57720629-57720648 | MsG0280009767.01.T01:CDS | 40.0% |
| !! | GAGAGCACAAGCTAGATTTT+GGG | - | Chr2:57720877-57720896 | MsG0280009767.01.T01:CDS | 40.0% |
| !! | TATTTTGGAGGAGACAGACT+TGG | - | Chr2:57721686-57721705 | MsG0280009767.01.T01:CDS | 40.0% |
| !! | TGAGAGCACAAGCTAGATTT+TGG | - | Chr2:57720876-57720895 | MsG0280009767.01.T01:CDS | 40.0% |
| !! | TTGCCATTGTGGATTAGAAC+CGG | + | Chr2:57720770-57720789 | None:intergenic | 40.0% |
| !!! | AGGTGGTTTTGTTAGGTTTC+TGG | - | Chr2:57720615-57720634 | MsG0280009767.01.T01:CDS | 40.0% |
| ACATACACAAGTGGAAGGAC+GGG | - | Chr2:57721009-57721028 | MsG0280009767.01.T01:intron | 45.0% | |
| ACCAACTGTAGAATGGGACT+AGG | + | Chr2:57721727-57721746 | None:intergenic | 45.0% | |
| AGAACAAGAAGCTGCCAAGA+AGG | - | Chr2:57721613-57721632 | MsG0280009767.01.T01:CDS | 45.0% | |
| AGTGCCCTAAGTTCATTGCT+TGG | - | Chr2:57721789-57721808 | MsG0280009767.01.T01:CDS | 45.0% | |
| AGTTCATTGCTTGGGCTAAG+AGG | - | Chr2:57721798-57721817 | MsG0280009767.01.T01:CDS | 45.0% | |
| CACATACACAAGTGGAAGGA+CGG | - | Chr2:57721008-57721027 | MsG0280009767.01.T01:intron | 45.0% | |
| CCAACTGTAGAATGGGACTA+GGG | + | Chr2:57721726-57721745 | None:intergenic | 45.0% | |
| GTCTCTTCCTAACCAGCATA+AGG | - | Chr2:57721847-57721866 | MsG0280009767.01.T01:CDS | 45.0% | |
| GTGCCCTAAGTTCATTGCTT+GGG | - | Chr2:57721790-57721809 | MsG0280009767.01.T01:CDS | 45.0% | |
| TAGGGATCAGAAGGCAACAA+CGG | + | Chr2:57720854-57720873 | None:intergenic | 45.0% | |
| TGAACTCGTAGACCTTATGC+TGG | + | Chr2:57721862-57721881 | None:intergenic | 45.0% | |
| ! | ACCGGGATTTTCTTGTGAAC+CGG | + | Chr2:57720752-57720771 | None:intergenic | 45.0% |
| ! | ACTTGACACCCTTTTCAGCA+AGG | + | Chr2:57720673-57720692 | None:intergenic | 45.0% |
| ! | ATCCCGGTTCTAATCCACAA+TGG | - | Chr2:57720764-57720783 | MsG0280009767.01.T01:CDS | 45.0% |
| ! | ATGCTGGTTAGGAAGAGACT+TGG | + | Chr2:57721846-57721865 | None:intergenic | 45.0% |
| ! | CTGGGAGACAAGGCTTATTT+TGG | - | Chr2:57721671-57721690 | MsG0280009767.01.T01:CDS | 45.0% |
| ! | CTTGACACCCTTTTCAGCAA+GGG | + | Chr2:57720672-57720691 | None:intergenic | 45.0% |
| ! | TCGTAGACCTTATGCTGGTT+AGG | + | Chr2:57721857-57721876 | None:intergenic | 45.0% |
| !! | TGCCATTGTGGATTAGAACC+GGG | + | Chr2:57720769-57720788 | None:intergenic | 45.0% |
| !!! | GCTGATGAGGTGGTTTTGTT+AGG | - | Chr2:57720608-57720627 | MsG0280009767.01.T01:CDS | 45.0% |
| !!! | GGAGACAAGGCTTATTTTGG+AGG | - | Chr2:57721674-57721693 | MsG0280009767.01.T01:CDS | 45.0% |
| CCCGGTTCACAAGAAAATCC+CGG | - | Chr2:57720748-57720767 | MsG0280009767.01.T01:CDS | 50.0% | |
| CCCTAGTCCCATTCTACAGT+TGG | - | Chr2:57721723-57721742 | MsG0280009767.01.T01:CDS | 50.0% | |
| ! | CCGGGATTTTCTTGTGAACC+GGG | + | Chr2:57720751-57720770 | None:intergenic | 50.0% |
| ! | CTAGCTTGTGCTCTCAGGTA+GGG | + | Chr2:57720872-57720891 | None:intergenic | 50.0% |
| ! | TCTAGCTTGTGCTCTCAGGT+AGG | + | Chr2:57720873-57720892 | None:intergenic | 50.0% |
| ! | TGGAGGAGACAGACTTGGTT+TGG | - | Chr2:57721691-57721710 | MsG0280009767.01.T01:CDS | 50.0% |
| GCTCTCAGGTAGGGATCAGA+AGG | + | Chr2:57720863-57720882 | None:intergenic | 55.0% | |
| GGAGAAACAGCTGGGAGACA+AGG | - | Chr2:57721661-57721680 | MsG0280009767.01.T01:CDS | 55.0% | |
| TACACAAGTGGAAGGACGGG+TGG | - | Chr2:57721012-57721031 | MsG0280009767.01.T01:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 57720605 | 57721916 | 57720605 | ID=MsG0280009767.01;Name=MsG0280009767.01 |
| Chr2 | mRNA | 57720605 | 57721916 | 57720605 | ID=MsG0280009767.01.T01;Parent=MsG0280009767.01;Name=MsG0280009767.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|220 |
| Chr2 | exon | 57721602 | 57721916 | 57721602 | ID=MsG0280009767.01.T01:exon:6157;Parent=MsG0280009767.01.T01 |
| Chr2 | exon | 57720605 | 57720952 | 57720605 | ID=MsG0280009767.01.T01:exon:6156;Parent=MsG0280009767.01.T01 |
| Chr2 | CDS | 57721602 | 57721916 | 57721602 | ID=MsG0280009767.01.T01:cds;Parent=MsG0280009767.01.T01 |
| Chr2 | CDS | 57720605 | 57720952 | 57720605 | ID=MsG0280009767.01.T01:cds;Parent=MsG0280009767.01.T01 |
| Gene Sequence |
| Protein sequence |
