AlfalfaGEDB | MsG0280010561.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010561.01

KEGG: -
GO: GO:0016787
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010561.01.T01	KAF8403383.1	77.922	154	4	1	21	174	929	1052	2.92E-74	251

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010561.01.T01	Q9SHS7	76.51	149	5	1	26	174	888	1006	6.91E-74	242

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010561.01.T01	A0A0R0H4W2	79.866	149	0	1	26	174	646	764	3.59e-77	254

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049059.01	MsG0280010561.01	0.802411	5.682470e-49	2.781712e-46
MsG0180000293.01	MsG0280010561.01	0.850999	1.180890e-60	2.356261e-57
MsG0280010561.01	MsG0380014365.01	0.816241	6.119181e-52	4.312763e-49
MsG0280010561.01	MsG0380015922.01	0.806243	9.043910e-50	4.884588e-47
MsG0280010561.01	MsG0380017403.01	0.802141	6.459562e-49	3.140261e-46
MsG0280010561.01	MsG0480018126.01	0.801735	7.825776e-49	3.765306e-46
MsG0280010561.01	MsG0480020065.01	0.805618	1.223768e-49	6.504257e-47
MsG0280010561.01	MsG0480020416.01	0.830628	2.638244e-55	2.795004e-52
MsG0280010561.01	MsG0480023245.01	0.805335	1.403210e-49	7.403902e-47
MsG0280010561.01	MsG0580025134.01	0.808802	2.589632e-50	1.494774e-47
MsG0280010561.01	MsG0580025989.01	0.832226	1.066442e-55	1.184488e-52
MsG0280010561.01	MsG0580027345.01	0.801648	8.153752e-49	3.914509e-46
MsG0280010561.01	MsG0580028185.01	0.824564	7.539001e-54	6.702491e-51
MsG0280010561.01	MsG0580029658.01	0.804579	2.018873e-49	1.044712e-46
MsG0280010561.01	MsG0580029661.01	0.820069	8.333703e-53	6.527518e-50
MsG0280010561.01	MsG0680035740.01	0.804275	2.335461e-49	1.199127e-46
MsG0280010561.01	MsG0780037503.01	0.800071	1.711404e-48	7.894939e-46
MsG0280010561.01	MsG0780037895.01	0.800159	1.642199e-48	7.592286e-46
MsG0280010561.01	MsG0780040360.01	0.861276	1.149622e-63	3.235325e-60
MsG0280010561.01	MsG0780040691.01	0.822653	2.110892e-53	1.777754e-50
MsG0280010561.01	MsG0780041399.01	0.830376	3.040449e-55	3.197705e-52
MsG0280010561.01	MsG0780041754.01	0.808219	3.449122e-50	1.960842e-47
MsG0280010561.01	MsG0880041969.01	0.816924	4.302862e-52	3.089837e-49
MsG0280010561.01	MsG0880042848.01	0.807755	4.328481e-50	2.430879e-47
MsG0280010561.01	MsG0880043082.01	0.802376	5.777288e-49	2.825686e-46
MsG0280010561.01	MsG0880043745.01	0.802093	6.607642e-49	3.208410e-46
MsG0280010561.01	MsG0880046113.01	0.802253	6.125454e-49	2.986448e-46
MsG0280010561.01	MsG0880046733.01	0.818935	1.511447e-52	1.147161e-49
MsG0280010561.01	MsG0880047590.01	0.824898	6.289928e-54	5.646023e-51
MsG0280007071.01	MsG0280010561.01	0.807578	4.721214e-50	2.639033e-47
MsG0280007715.01	MsG0280010561.01	0.812113	4.991910e-51	3.146120e-48
MsG0280008286.01	MsG0280010561.01	0.807239	5.570619e-50	3.086753e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010561.01.T01	MTR_2g087530	79.866	149	0	1	26	174	764	882	5.69e-80	254
MsG0280010561.01.T01	MTR_2g087530	79.866	149	0	1	26	174	877	995	2.05e-79	254
MsG0280010561.01.T01	MTR_4g050540	77.852	149	2	2	26	174	882	999	5.33e-76	245
MsG0280010561.01.T01	MTR_1g089370	63.758	149	24	1	26	174	761	879	3.22e-49	170
MsG0280010561.01.T01	MTR_1g074880	41.053	95	54	1	27	119	171	265	6.55e-16	73.6
MsG0280010561.01.T01	MTR_1g074880	48.571	70	36	0	27	96	171	240	7.85e-16	73.2
MsG0280010561.01.T01	MTR_8g005010	47.887	71	37	0	26	96	223	293	9.83e-16	73.2
MsG0280010561.01.T01	MTR_5g081230	47.143	70	37	0	26	95	242	311	1.03e-15	73.6
MsG0280010561.01.T01	MTR_1g074880	39.048	105	58	2	17	119	220	320	1.06e-15	73.6
MsG0280010561.01.T01	MTR_1g074880	45.000	80	40	1	17	96	220	295	1.11e-15	73.6
MsG0280010561.01.T01	MTR_7g112870	43.011	93	47	2	14	106	221	307	4.79e-15	71.6
MsG0280010561.01.T01	MTR_1g079950	43.038	79	41	1	17	95	249	323	1.90e-14	70.1
MsG0280010561.01.T01	MTR_1g079950	43.038	79	41	1	17	95	220	294	1.97e-14	70.1
MsG0280010561.01.T01	MTR_1g079950	43.038	79	41	1	17	95	194	268	2.01e-14	69.7
MsG0280010561.01.T01	MTR_1g079950	43.038	79	41	1	17	95	220	294	2.08e-14	70.1
MsG0280010561.01.T01	MTR_1g079950	43.038	79	41	1	17	95	220	294	2.18e-14	69.7
MsG0280010561.01.T01	MTR_7g116380	41.772	79	42	1	17	95	220	294	2.34e-14	69.7
MsG0280010561.01.T01	MTR_7g116380	41.772	79	42	1	17	95	220	294	2.84e-14	69.7
MsG0280010561.01.T01	MTR_8g098980	47.887	71	33	1	17	87	221	287	4.50e-14	68.9
MsG0280010561.01.T01	MTR_1g043240	42.500	80	42	1	17	96	220	295	6.28e-14	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010561.01.T01	AT2G27210	76.510	149	5	1	26	174	883	1001	6.76e-75	242
MsG0280010561.01.T01	AT2G27210	76.510	149	5	1	26	174	888	1006	7.05e-75	242
MsG0280010561.01.T01	AT1G08420	75.333	150	6	2	26	174	899	1018	1.92e-72	235
MsG0280010561.01.T01	AT1G08420	75.333	150	6	2	26	174	894	1013	2.03e-72	235
MsG0280010561.01.T01	AT4G03080	63.087	149	25	1	26	174	763	881	1.28e-53	182
MsG0280010561.01.T01	AT1G03445	47.799	159	49	3	4	161	665	790	3.31e-37	135
MsG0280010561.01.T01	AT1G03445	56.557	122	49	2	4	124	665	783	4.40e-37	135
MsG0280010561.01.T01	AT5G27840	43.333	90	45	1	14	103	225	308	4.25e-17	77.4
MsG0280010561.01.T01	AT5G27840	43.333	90	45	1	14	103	225	308	4.25e-17	77.4
MsG0280010561.01.T01	AT5G27840	43.333	90	45	1	14	103	225	308	4.70e-17	77.4
MsG0280010561.01.T01	AT5G27840	43.333	90	45	1	14	103	225	308	4.70e-17	77.4
MsG0280010561.01.T01	AT3G05580	41.111	90	47	1	14	103	225	308	6.53e-16	73.9
MsG0280010561.01.T01	AT5G59160	47.143	70	37	0	26	95	235	304	1.49e-15	73.2
MsG0280010561.01.T01	AT5G59160	47.143	70	37	0	26	95	235	304	1.49e-15	73.2
MsG0280010561.01.T01	AT5G59160	47.143	70	37	0	26	95	235	304	1.49e-15	73.2
MsG0280010561.01.T01	AT2G39840	45.714	70	38	0	26	95	239	308	3.11e-15	72.4
MsG0280010561.01.T01	AT2G29400	41.772	79	42	1	17	95	236	310	9.19e-15	70.9
MsG0280010561.01.T01	AT3G46820	41.250	80	43	1	17	96	230	305	1.29e-14	70.5
MsG0280010561.01.T01	AT4G11240	46.479	71	34	1	17	87	221	287	1.01e-13	68.2
MsG0280010561.01.T01	AT5G43380	49.296	71	32	1	17	87	220	286	2.96e-13	66.6
MsG0280010561.01.T01	AT1G64040	46.479	71	34	1	17	87	221	287	3.18e-13	66.6
MsG0280010561.01.T01	AT5G43380	49.296	71	32	1	17	87	220	286	3.26e-13	66.6
MsG0280010561.01.T01	AT5G43380	49.296	71	32	1	17	87	220	286	3.97e-13	66.2

Find 37 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGTATCATGCTCATTTAAA+TGG	0.146276	2:-71522338	MsG0280010561.01.T01:CDS
TGCCGTTCTGGTGACGTTTC+TGG	0.228839	2:+71521985	None:intergenic
TGCTGGAGCGATATTGGTTT+TGG	0.235273	2:-71522067	MsG0280010561.01.T01:CDS
GAACCTCGGTCGTTTGTAAC+TGG	0.274342	2:+71521704	None:intergenic
GGTAGAGGATGAATCAATTT+AGG	0.304308	2:+71522024	None:intergenic
AAATAATGCTGGAGCGATAT+TGG	0.331344	2:-71522073	MsG0280010561.01.T01:CDS
GGTATCATGCTCATTTAAAT+GGG	0.349972	2:-71522337	MsG0280010561.01.T01:CDS
GTATCTTCAATATGCCGTTC+TGG	0.408668	2:+71521973	None:intergenic
GAAGAAATTGCAGGAGGTAG+AGG	0.444262	2:+71522009	None:intergenic
AACCTCGGTCGTTTGTAACT+GGG	0.447493	2:+71521705	None:intergenic
CAAAATTCCATGACTCGATC+AGG	0.454314	2:+71522300	None:intergenic
AGTTGGTGTTGGTGGTCTGT+TGG	0.480071	2:+71521739	None:intergenic
ACGACCGAGGTTCACTCGCT+TGG	0.480448	2:-71521694	MsG0280010561.01.T01:CDS
CATGTGCACGGACAATTAGC+TGG	0.487650	2:+71522262	None:intergenic
CGACCTCTAGTTGGTGTTGG+TGG	0.503074	2:+71521731	None:intergenic
GTTTCTGGTGAAGAAATTGC+AGG	0.510520	2:+71522000	None:intergenic
GGCCGACCTCTAGTTGGTGT+TGG	0.518704	2:+71521728	None:intergenic
AAAGTGTGATCAAATGTCCC+TGG	0.529867	2:+71522208	None:intergenic
ATAGGTACTGCAAATAATGC+TGG	0.534482	2:-71522084	MsG0280010561.01.T01:intron
TATTGAAGATACATGGATGC+AGG	0.553399	2:-71521962	MsG0280010561.01.T01:CDS
GCACATGAATGTGTGATGGA+TGG	0.567213	2:-71522246	MsG0280010561.01.T01:CDS
AACGGCATATTGAAGATACA+TGG	0.569008	2:-71521969	MsG0280010561.01.T01:CDS
ACCTCGGTCGTTTGTAACTG+GGG	0.574594	2:+71521706	None:intergenic
CACATGAATGTGTGATGGAT+GGG	0.582245	2:-71522245	MsG0280010561.01.T01:CDS
TTCTCGGCTACAAATTATTG+CGG	0.590452	2:-71522186	MsG0280010561.01.T01:intron
ATATCCAAGCGAGTGAACCT+CGG	0.592554	2:+71521690	None:intergenic
ACTTCAGCCTGATCGAGTCA+TGG	0.600991	2:-71522307	MsG0280010561.01.T01:CDS
AAGTGTGATCAAATGTCCCT+GGG	0.601391	2:+71522209	None:intergenic
CCGTGCACATGAATGTGTGA+TGG	0.616916	2:-71522250	MsG0280010561.01.T01:CDS
CCATCACACATTCATGTGCA+CGG	0.624398	2:+71522250	None:intergenic
CACCAGAAACGTCACCAGAA+CGG	0.630921	2:-71521987	MsG0280010561.01.T01:CDS
GTATCATGCTCATTTAAATG+GGG	0.638485	2:-71522336	MsG0280010561.01.T01:CDS
CACCAACACCAACTAGAGGT+CGG	0.645416	2:-71521730	MsG0280010561.01.T01:CDS
CCTCGGTCGTTTGTAACTGG+GGG	0.647672	2:+71521707	None:intergenic
AGACCACCAACACCAACTAG+AGG	0.687730	2:-71521734	MsG0280010561.01.T01:CDS
TCTGGTGAAGAAATTGCAGG+AGG	0.689270	2:+71522003	None:intergenic
TCAATTTAGGAACTACAACA+AGG	0.730229	2:+71522037	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTGATAGTTTTTTATACA+TGG	-	Chr2:71521705-71521724	MsG0280010561.01.T01:CDS	15.0%
!!!	TTTTAATGAAAAATATCTGA+AGG	+	Chr2:71522131-71522150	None:intergenic	15.0%
!!	AAAAAACTATCAAATAGCAT+AGG	+	Chr2:71521700-71521719	None:intergenic	20.0%
!!!	TATACAACATAGTTTGTTAT+AGG	-	Chr2:71522203-71522222	MsG0280010561.01.T01:CDS	20.0%
!!!	TGATTTTGTTGTTTAAATAG+TGG	+	Chr2:71522167-71522186	None:intergenic	20.0%
!	TTGAAAGAAAATGTGATCTA+AGG	+	Chr2:71521935-71521954	None:intergenic	25.0%
!!	AAAGTTAAAATTTTACGTGC+AGG	-	Chr2:71522292-71522311	MsG0280010561.01.T01:CDS	25.0%
	GGTATCATGCTCATTTAAAT+GGG	-	Chr2:71521726-71521745	MsG0280010561.01.T01:CDS	30.0%
	GTATCATGCTCATTTAAATG+GGG	-	Chr2:71521727-71521746	MsG0280010561.01.T01:CDS	30.0%
	TCAATTTAGGAACTACAACA+AGG	+	Chr2:71522029-71522048	None:intergenic	30.0%
	TGGTATCATGCTCATTTAAA+TGG	-	Chr2:71521725-71521744	MsG0280010561.01.T01:CDS	30.0%
!	ACATTTGATCACACTTTTCT+CGG	-	Chr2:71521861-71521880	MsG0280010561.01.T01:intron	30.0%
	AAATAATGCTGGAGCGATAT+TGG	-	Chr2:71521990-71522009	MsG0280010561.01.T01:CDS	35.0%
	AACGGCATATTGAAGATACA+TGG	-	Chr2:71522094-71522113	MsG0280010561.01.T01:intron	35.0%
	AGCATTATTTGCAGTACCTA+TGG	+	Chr2:71521980-71521999	None:intergenic	35.0%
	GCATTATTTGCAGTACCTAT+GGG	+	Chr2:71521979-71521998	None:intergenic	35.0%
	GGTAGAGGATGAATCAATTT+AGG	+	Chr2:71522042-71522061	None:intergenic	35.0%
	TATTGAAGATACATGGATGC+AGG	-	Chr2:71522101-71522120	MsG0280010561.01.T01:intron	35.0%
	TTCTCGGCTACAAATTATTG+CGG	-	Chr2:71521877-71521896	MsG0280010561.01.T01:intron	35.0%
!	ATAGGTACTGCAAATAATGC+TGG	-	Chr2:71521979-71521998	MsG0280010561.01.T01:CDS	35.0%
	AAAGTGTGATCAAATGTCCC+TGG	+	Chr2:71521858-71521877	None:intergenic	40.0%
	AAGTGTGATCAAATGTCCCT+GGG	+	Chr2:71521857-71521876	None:intergenic	40.0%
	ACCAACTATTCACGTAGATG+TGG	+	Chr2:71522247-71522266	None:intergenic	40.0%
	ACCACATCTACGTGAATAGT+TGG	-	Chr2:71522243-71522262	MsG0280010561.01.T01:CDS	40.0%
	CAAAATTCCATGACTCGATC+AGG	+	Chr2:71521766-71521785	None:intergenic	40.0%
	GTATCTTCAATATGCCGTTC+TGG	+	Chr2:71522093-71522112	None:intergenic	40.0%
!!	CACATGAATGTGTGATGGAT+GGG	-	Chr2:71521818-71521837	MsG0280010561.01.T01:intron	40.0%
!!	GTTTCTGGTGAAGAAATTGC+AGG	+	Chr2:71522066-71522085	None:intergenic	40.0%
	AACCTCGGTCGTTTGTAACT+GGG	+	Chr2:71522361-71522380	None:intergenic	45.0%
	ACTCTTCCAGTTGTTCCCAT+AGG	-	Chr2:71521961-71521980	MsG0280010561.01.T01:CDS	45.0%
	ATATCCAAGCGAGTGAACCT+CGG	+	Chr2:71522376-71522395	None:intergenic	45.0%
	CCATCACACATTCATGTGCA+CGG	+	Chr2:71521816-71521835	None:intergenic	45.0%
	GAAGAAATTGCAGGAGGTAG+AGG	+	Chr2:71522057-71522076	None:intergenic	45.0%
	TCTGGTGAAGAAATTGCAGG+AGG	+	Chr2:71522063-71522082	None:intergenic	45.0%
!	GCACATGAATGTGTGATGGA+TGG	-	Chr2:71521817-71521836	MsG0280010561.01.T01:intron	45.0%
!!!	GCTGGAGCGATATTGGTTTT+GGG	-	Chr2:71521997-71522016	MsG0280010561.01.T01:CDS	45.0%
!!!	TGCTGGAGCGATATTGGTTT+TGG	-	Chr2:71521996-71522015	MsG0280010561.01.T01:CDS	45.0%
	ACCTCGGTCGTTTGTAACTG+GGG	+	Chr2:71522360-71522379	None:intergenic	50.0%
	ACTTCAGCCTGATCGAGTCA+TGG	-	Chr2:71521756-71521775	MsG0280010561.01.T01:CDS	50.0%
	AGACCACCAACACCAACTAG+AGG	-	Chr2:71522329-71522348	MsG0280010561.01.T01:CDS	50.0%
	CACCAACACCAACTAGAGGT+CGG	-	Chr2:71522333-71522352	MsG0280010561.01.T01:CDS	50.0%
	CACCAGAAACGTCACCAGAA+CGG	-	Chr2:71522076-71522095	MsG0280010561.01.T01:CDS	50.0%
	CATGTGCACGGACAATTAGC+TGG	+	Chr2:71521804-71521823	None:intergenic	50.0%
	CCGTGCACATGAATGTGTGA+TGG	-	Chr2:71521813-71521832	MsG0280010561.01.T01:intron	50.0%
	GAACCTCGGTCGTTTGTAAC+TGG	+	Chr2:71522362-71522381	None:intergenic	50.0%
	GCAGTACCTATGGGAACAAC+TGG	+	Chr2:71521970-71521989	None:intergenic	50.0%
!!	AGTTGGTGTTGGTGGTCTGT+TGG	+	Chr2:71522327-71522346	None:intergenic	50.0%
	CCTCGGTCGTTTGTAACTGG+GGG	+	Chr2:71522359-71522378	None:intergenic	55.0%
!	GGGTTTGAGCGTTTTGCCCA+GGG	-	Chr2:71521838-71521857	MsG0280010561.01.T01:intron	55.0%
!	TGCCGTTCTGGTGACGTTTC+TGG	+	Chr2:71522081-71522100	None:intergenic	55.0%
!	TGGGTTTGAGCGTTTTGCCC+AGG	-	Chr2:71521837-71521856	MsG0280010561.01.T01:intron	55.0%
!!	CGACCTCTAGTTGGTGTTGG+TGG	+	Chr2:71522335-71522354	None:intergenic	55.0%
	ACGACCGAGGTTCACTCGCT+TGG	-	Chr2:71522369-71522388	MsG0280010561.01.T01:CDS	60.0%
	CCCCCAGTTACAAACGACCG+AGG	-	Chr2:71522356-71522375	MsG0280010561.01.T01:CDS	60.0%
!	GGCCGACCTCTAGTTGGTGT+TGG	+	Chr2:71522338-71522357	None:intergenic	60.0%
	ACTGGGGGCCGACCTCTAGT+TGG	+	Chr2:71522344-71522363	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	71521688	71522397	71521688	ID=MsG0280010561.01;Name=MsG0280010561.01
Chr2	mRNA	71521688	71522397	71521688	ID=MsG0280010561.01.T01;Parent=MsG0280010561.01;Name=MsG0280010561.01.T01;_AED=0.48;_eAED=0.56;_QI=0\|0.5\|0\|1\|0.5\|0.33\|3\|0\|174
Chr2	exon	71522187	71522397	71522187	ID=MsG0280010561.01.T01:exon:15127;Parent=MsG0280010561.01.T01
Chr2	exon	71521925	71522102	71521925	ID=MsG0280010561.01.T01:exon:15126;Parent=MsG0280010561.01.T01
Chr2	exon	71521688	71521823	71521688	ID=MsG0280010561.01.T01:exon:15125;Parent=MsG0280010561.01.T01
Chr2	CDS	71522187	71522397	71522187	ID=MsG0280010561.01.T01:cds;Parent=MsG0280010561.01.T01
Chr2	CDS	71521925	71522102	71521925	ID=MsG0280010561.01.T01:cds;Parent=MsG0280010561.01.T01
Chr2	CDS	71521688	71521823	71521688	ID=MsG0280010561.01.T01:cds;Parent=MsG0280010561.01.T01

Gene Sequence

>MsG0280010561.01.T01

ATGTTTTTACCTATGCTATTTGATAGTTTTTTATACATGGTATCATGCTCATTTAAATGGGGTTCTGTACTTCAGCCTGATCGAGTCATGGAATTTTGCAACAACAACGATCTCCAGCTAATTGTCCGTGCACATGAATGTGTGATGGATGGGTTTGAGCGTTTTGCCCAGGGACATTTGATCACACTTTTCTCGGCTACAAATTATTGCGGTACTGCAAATAATGCTGGAGCGATATTGGTTTTGGGTAGAGACCTTGTTGTAGTTCCTAAATTGATTCATCCTCTACCTCCTGCAATTTCTTCACCAGAAACGTCACCAGAACGGCATATTGAAGATACATGGATGCAGGTACTCCTTCAGATATTTTTCATTAAAAAAAAAACATATTGGTGTAACTCATTATTATGTTGCTTACAAAAGTTAAAATTTTACGTGCAGGAGTTGAATGCCAACAGACCACCAACACCAACTAGAGGTCGGCCCCCAGTTACAAACGACCGAGGTTCACTCGCTTGGATATAG

Protein sequence

>MsG0280010561.01.T01

MFLPMLFDSFLYMVSCSFKWGSVLQPDRVMEFCNNNDLQLIVRAHECVMDGFERFAQGHLITLFSATNYCGTANNAGAILVLGRDLVVVPKLIHPLPPAISSPETSPERHIEDTWMQVLLQIFFIKKKTYWCNSLLCCLQKLKFYVQELNANRPPTPTRGRPPVTNDRGSLAWI*