AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043082.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043082.01.T01 MTR_8g028180 98.630 146 2 0 1 146 1 146 3.38e-101 286
MsG0880043082.01.T01 MTR_3g005520 83.333 150 21 1 1 146 1 150 2.42e-77 226
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043082.01.T01 AT2G04520 87.671 146 17 1 1 146 1 145 8.61e-85 245
MsG0880043082.01.T01 AT5G35680 86.986 146 18 1 1 146 1 145 7.99e-80 232
MsG0880043082.01.T01 AT5G35680 86.986 146 18 1 1 146 1 145 7.99e-80 232
MsG0880043082.01.T01 AT5G35680 93.277 119 8 0 1 119 1 119 6.65e-78 228

Find 46 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGGTTGCGTGATTATC+AGG 0.308178 8:-18665105 MsG0880043082.01.T01:CDS
TCATTAAGCCTCACACCTTC+AGG 0.319525 8:+18665011 None:intergenic
GCTGGTGATATTATTCTTGT+TGG 0.325909 8:-18665122 MsG0880043082.01.T01:CDS
CCTCCCTTTCCCTTGTTCTT+CGG 0.331257 8:+18665335 None:intergenic
GGAATTGGTGGTGGGATTGA+TGG 0.360212 8:-18664987 MsG0880043082.01.T01:CDS
CTGGTGATATTATTCTTGTT+GGG 0.374720 8:-18665121 MsG0880043082.01.T01:CDS
GAGGATATTGATAAGATTTG+AGG 0.399236 8:-18664918 None:intergenic
GAAAGATGCACAAGAAGGTT+TGG 0.408452 8:-18665151 MsG0880043082.01.T01:CDS
CTTTCCCTTGTTCTTCGGCA+TGG 0.425552 8:+18665340 None:intergenic
GAATTGGTGGTGGGATTGAT+GGG 0.434528 8:-18664986 MsG0880043082.01.T01:CDS
TTCAACAACCTCGCCTCATC+CGG 0.452149 8:+18665050 None:intergenic
TTATATTGAGTTTGAGGATG+AGG 0.463979 8:-18664937 MsG0880043082.01.T01:CDS
GCACAGGTTCTTCGTATGCT+TGG 0.465282 8:-18665233 MsG0880043082.01.T01:CDS
GGGGATGATGATGAGGGTGA+TGG 0.471229 8:-18664966 MsG0880043082.01.T01:CDS
GGGATGATGATGAGGGTGAT+GGG 0.477444 8:-18664965 MsG0880043082.01.T01:CDS
AGGATATTGATAAGATTTGA+GGG 0.478420 8:-18664917 None:intergenic
GAATGATTATATTGAGTTTG+AGG 0.491210 8:-18664943 MsG0880043082.01.T01:CDS
GTGAGGCTTAATGAAGGAAT+TGG 0.513375 8:-18665002 MsG0880043082.01.T01:CDS
CTTAATGAAGGAATTGGTGG+TGG 0.513624 8:-18664996 MsG0880043082.01.T01:CDS
GCGTGATTATCAGGATGATA+AGG 0.518535 8:-18665096 MsG0880043082.01.T01:CDS
GGATGAGGCGAGGTTGTTGA+AGG 0.519284 8:-18665048 MsG0880043082.01.T01:CDS
CCTTCTTGTGCATCTTTCCA+CGG 0.526928 8:+18665156 None:intergenic
ATGCCGAAGAACAAGGGAAA+GGG 0.538255 8:-18665338 MsG0880043082.01.T01:CDS
GATTGATGGGGATGATGATG+AGG 0.545936 8:-18664973 MsG0880043082.01.T01:CDS
TTCAACCATGCCGAAGAACA+AGG 0.546102 8:-18665345 None:intergenic
GTTCTTCGTATGCTTGGAAA+CGG 0.546331 8:-18665227 MsG0880043082.01.T01:CDS
CTTGAAGTATATGCCGGATG+AGG 0.557673 8:-18665063 MsG0880043082.01.T01:CDS
AAGAAGGTTTGGATTGCTGC+TGG 0.565369 8:-18665140 MsG0880043082.01.T01:CDS
ATTGATGGGGATGATGATGA+GGG 0.568262 8:-18664972 MsG0880043082.01.T01:CDS
CTTTGTTCCATCGATACACA+TGG 0.573074 8:+18665193 None:intergenic
GGAGGAAAGAATCGCAAGAG+AGG 0.578624 8:-18665317 MsG0880043082.01.T01:CDS
CCGTGGAAAGATGCACAAGA+AGG 0.600822 8:-18665156 MsG0880043082.01.T01:CDS
CATGCCGAAGAACAAGGGAA+AGG 0.602323 8:-18665339 MsG0880043082.01.T01:CDS
TTAATGAAGGAATTGGTGGT+GGG 0.613970 8:-18664995 MsG0880043082.01.T01:CDS
AAGCGTCTCTGTCATATCCG+TGG 0.616715 8:-18665173 MsG0880043082.01.T01:CDS
GCGTATAGCGAGCTTCCTGA+AGG 0.618073 8:-18665026 MsG0880043082.01.T01:CDS
GAAGGTGTGAGGCTTAATGA+AGG 0.621479 8:-18665008 MsG0880043082.01.T01:CDS
TGTGAAGCCATGTGTATCGA+TGG 0.627821 8:-18665200 MsG0880043082.01.T01:CDS
CCGAAGAACAAGGGAAAGGG+AGG 0.634335 8:-18665335 MsG0880043082.01.T01:CDS
AATTGGTGGTGGGATTGATG+GGG 0.634571 8:-18664985 MsG0880043082.01.T01:CDS
AGGCTTAATGAAGGAATTGG+TGG 0.634691 8:-18664999 MsG0880043082.01.T01:CDS
TCAACCATGCCGAAGAACAA+GGG 0.635918 8:-18665344 None:intergenic
GCGAGCTTCCTGAAGGTGTG+AGG 0.643888 8:-18665019 MsG0880043082.01.T01:CDS
AGATGGTCAAGAATACGCAC+AGG 0.647023 8:-18665249 MsG0880043082.01.T01:CDS
TGTGATCTTGAAGTATATGC+CGG 0.651550 8:-18665069 MsG0880043082.01.T01:CDS
AGTATATGCCGGATGAGGCG+AGG 0.665508 8:-18665058 MsG0880043082.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GAATGATTATATTGAGTTTG+AGG - Chr8:18665315-18665334 MsG0880043082.01.T01:CDS 25.0%
CTGGTGATATTATTCTTGTT+GGG - Chr8:18665137-18665156 MsG0880043082.01.T01:CDS 30.0%
TTATATTGAGTTTGAGGATG+AGG - Chr8:18665321-18665340 MsG0880043082.01.T01:CDS 30.0%
GCTGGTGATATTATTCTTGT+TGG - Chr8:18665136-18665155 MsG0880043082.01.T01:CDS 35.0%
TGTGATCTTGAAGTATATGC+CGG - Chr8:18665189-18665208 MsG0880043082.01.T01:CDS 35.0%
! TTAATGAAGGAATTGGTGGT+GGG - Chr8:18665263-18665282 MsG0880043082.01.T01:CDS 35.0%
AGGCTTAATGAAGGAATTGG+TGG - Chr8:18665259-18665278 MsG0880043082.01.T01:CDS 40.0%
CTTAATGAAGGAATTGGTGG+TGG - Chr8:18665262-18665281 MsG0880043082.01.T01:CDS 40.0%
CTTTGTTCCATCGATACACA+TGG + Chr8:18665068-18665087 None:intergenic 40.0%
GAAAGATGCACAAGAAGGTT+TGG - Chr8:18665107-18665126 MsG0880043082.01.T01:CDS 40.0%
GCGTGATTATCAGGATGATA+AGG - Chr8:18665162-18665181 MsG0880043082.01.T01:CDS 40.0%
GTGAGGCTTAATGAAGGAAT+TGG - Chr8:18665256-18665275 MsG0880043082.01.T01:CDS 40.0%
GTTCTTCGTATGCTTGGAAA+CGG - Chr8:18665031-18665050 MsG0880043082.01.T01:CDS 40.0%
! ATTGATGGGGATGATGATGA+GGG - Chr8:18665286-18665305 MsG0880043082.01.T01:CDS 40.0%
!! AAAGCGTGAGCTTGTTTTCA+AGG - Chr8:18664985-18665004 MsG0880043082.01.T01:CDS 40.0%
!! GAGCTTGTTTTCAAGGAAGA+TGG - Chr8:18664992-18665011 MsG0880043082.01.T01:CDS 40.0%
AAGAAGGTTTGGATTGCTGC+TGG - Chr8:18665118-18665137 MsG0880043082.01.T01:CDS 45.0%
AGATGGTCAAGAATACGCAC+AGG - Chr8:18665009-18665028 MsG0880043082.01.T01:CDS 45.0%
ATGCCGAAGAACAAGGGAAA+GGG - Chr8:18664920-18664939 MsG0880043082.01.T01:CDS 45.0%
CCTTCTTGTGCATCTTTCCA+CGG + Chr8:18665105-18665124 None:intergenic 45.0%
CTTGAAGTATATGCCGGATG+AGG - Chr8:18665195-18665214 MsG0880043082.01.T01:CDS 45.0%
TCATTAAGCCTCACACCTTC+AGG + Chr8:18665250-18665269 None:intergenic 45.0%
TGTGAAGCCATGTGTATCGA+TGG - Chr8:18665058-18665077 MsG0880043082.01.T01:CDS 45.0%
! AAGCTCACGCTTTTCGTCAT+CGG + Chr8:18664978-18664997 None:intergenic 45.0%
! GAAGGTGTGAGGCTTAATGA+AGG - Chr8:18665250-18665269 MsG0880043082.01.T01:CDS 45.0%
! GATTGATGGGGATGATGATG+AGG - Chr8:18665285-18665304 MsG0880043082.01.T01:CDS 45.0%
! TGTTGGGTTGCGTGATTATC+AGG - Chr8:18665153-18665172 MsG0880043082.01.T01:CDS 45.0%
!! AATTGGTGGTGGGATTGATG+GGG - Chr8:18665273-18665292 MsG0880043082.01.T01:CDS 45.0%
!! GAATTGGTGGTGGGATTGAT+GGG - Chr8:18665272-18665291 MsG0880043082.01.T01:CDS 45.0%
AAGCGTCTCTGTCATATCCG+TGG - Chr8:18665085-18665104 MsG0880043082.01.T01:CDS 50.0%
CCGTGGAAAGATGCACAAGA+AGG - Chr8:18665102-18665121 MsG0880043082.01.T01:CDS 50.0%
CCTCCCTTTCCCTTGTTCTT+CGG + Chr8:18664926-18664945 None:intergenic 50.0%
GCACAGGTTCTTCGTATGCT+TGG - Chr8:18665025-18665044 MsG0880043082.01.T01:CDS 50.0%
GGAGGAAAGAATCGCAAGAG+AGG - Chr8:18664941-18664960 MsG0880043082.01.T01:CDS 50.0%
TTCAACAACCTCGCCTCATC+CGG + Chr8:18665211-18665230 None:intergenic 50.0%
! GGGATGATGATGAGGGTGAT+GGG - Chr8:18665293-18665312 MsG0880043082.01.T01:CDS 50.0%
!! GGAATTGGTGGTGGGATTGA+TGG - Chr8:18665271-18665290 MsG0880043082.01.T01:CDS 50.0%
AGTATATGCCGGATGAGGCG+AGG - Chr8:18665200-18665219 MsG0880043082.01.T01:CDS 55.0%
CCGAAGAACAAGGGAAAGGG+AGG - Chr8:18664923-18664942 MsG0880043082.01.T01:CDS 55.0%
GCGTATAGCGAGCTTCCTGA+AGG - Chr8:18665232-18665251 MsG0880043082.01.T01:CDS 55.0%
GGATGAGGCGAGGTTGTTGA+AGG - Chr8:18665210-18665229 MsG0880043082.01.T01:CDS 55.0%
! GGGGATGATGATGAGGGTGA+TGG - Chr8:18665292-18665311 MsG0880043082.01.T01:CDS 55.0%
! GCGAGCTTCCTGAAGGTGTG+AGG - Chr8:18665239-18665258 MsG0880043082.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr8 gene 18664920 18665360 18664920 ID=MsG0880043082.01;Name=MsG0880043082.01
Chr8 mRNA 18664920 18665360 18664920 ID=MsG0880043082.01.T01;Parent=MsG0880043082.01;Name=MsG0880043082.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|146
Chr8 exon 18664920 18665360 18664920 ID=MsG0880043082.01.T01:exon:4908;Parent=MsG0880043082.01.T01
Chr8 CDS 18664920 18665360 18664920 ID=MsG0880043082.01.T01:cds;Parent=MsG0880043082.01.T01
Gene Sequence

>MsG0880043082.01.T01

ATGCCGAAGAACAAGGGAAAGGGAGGAAAGAATCGCAAGAGAGGAAAGAACGAAGCCGATGACGAAAAGCGTGAGCTTGTTTTCAAGGAAGATGGTCAAGAATACGCACAGGTTCTTCGTATGCTTGGAAACGGTCGTTGTGAAGCCATGTGTATCGATGGAACAAAGCGTCTCTGTCATATCCGTGGAAAGATGCACAAGAAGGTTTGGATTGCTGCTGGTGATATTATTCTTGTTGGGTTGCGTGATTATCAGGATGATAAGGCTGATGTGATCTTGAAGTATATGCCGGATGAGGCGAGGTTGTTGAAGGCGTATAGCGAGCTTCCTGAAGGTGTGAGGCTTAATGAAGGAATTGGTGGTGGGATTGATGGGGATGATGATGAGGGTGATGGGAATGATTATATTGAGTTTGAGGATGAGGATATTGATAAGATTTGA

Protein sequence

>MsG0880043082.01.T01

MPKNKGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQVLRMLGNGRCEAMCIDGTKRLCHIRGKMHKKVWIAAGDIILVGLRDYQDDKADVILKYMPDEARLLKAYSELPEGVRLNEGIGGGIDGDDDEGDGNDYIEFEDEDIDKI*