Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010891.01.T01 | XP_003597210.2 | 98.068 | 207 | 4 | 0 | 1 | 207 | 87 | 293 | 7.35E-143 | 410 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010891.01.T01 | Q8LEE9 | 50 | 130 | 63 | 2 | 1 | 129 | 57 | 185 | 3.25E-35 | 127 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010891.01.T01 | G7IRP5 | 98.068 | 207 | 4 | 0 | 1 | 207 | 87 | 293 | 3.51e-143 | 410 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010891.01 | MsG0280010893.01 | 0.821389 | 4.145144e-53 | 3.367707e-50 |
| MsG0280010891.01 | MsG0480020552.01 | 0.880146 | 6.981598e-70 | 3.890044e-66 |
| MsG0280010891.01 | MsG0480020815.01 | 0.841502 | 4.570935e-58 | 6.729922e-55 |
| MsG0280010891.01 | MsG0480020817.01 | 0.830681 | 2.560721e-55 | 2.717304e-52 |
| MsG0280010891.01 | MsG0480020818.01 | 0.815230 | 1.028223e-51 | 7.048513e-49 |
| MsG0280010891.01 | MsG0480023113.01 | 0.890716 | 7.663592e-74 | 6.520388e-70 |
| MsG0280010891.01 | MsG0480023114.01 | 0.897539 | 1.274988e-76 | 1.447104e-72 |
| MsG0280010891.01 | MsG0480023115.01 | 0.846256 | 2.434271e-59 | 4.165086e-56 |
| MsG0280010891.01 | MsG0480023116.01 | 0.861414 | 1.043057e-63 | 2.949394e-60 |
| MsG0280010891.01 | MsG0780040592.01 | 0.887737 | 1.096725e-72 | 8.255733e-69 |
| MsG0280010891.01 | MsG0880042557.01 | 0.833883 | 4.129502e-56 | 4.819781e-53 |
| MsG0280010891.01 | MsG0880045339.01 | 0.870050 | 1.954569e-66 | 7.498531e-63 |
| MsG0280010891.01 | MsG0880047773.01 | 0.876019 | 1.946459e-68 | 9.289203e-65 |
| MsG0280009327.01 | MsG0280010891.01 | 0.826868 | 2.142366e-54 | 2.033974e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010891.01.T01 | MTR_2g093970 | 98.068 | 207 | 4 | 0 | 1 | 207 | 87 | 293 | 8.90e-147 | 410 |
| MsG0280010891.01.T01 | MTR_4g059790 | 68.500 | 200 | 55 | 3 | 1 | 199 | 91 | 283 | 5.15e-86 | 256 |
| MsG0280010891.01.T01 | MTR_4g059840 | 67.327 | 202 | 58 | 3 | 1 | 200 | 91 | 286 | 1.44e-85 | 255 |
| MsG0280010891.01.T01 | MTR_4g053380 | 67.500 | 200 | 62 | 1 | 1 | 200 | 95 | 291 | 9.58e-82 | 245 |
| MsG0280010891.01.T01 | MTR_2g017970 | 54.271 | 199 | 79 | 3 | 1 | 198 | 86 | 273 | 5.52e-64 | 199 |
| MsG0280010891.01.T01 | MTR_2g017960 | 52.709 | 203 | 84 | 3 | 1 | 202 | 86 | 277 | 4.02e-62 | 194 |
| MsG0280010891.01.T01 | MTR_2g017950 | 46.635 | 208 | 100 | 3 | 1 | 202 | 89 | 291 | 2.14e-56 | 181 |
| MsG0280010891.01.T01 | MTR_8g087450 | 62.308 | 130 | 49 | 0 | 1 | 130 | 95 | 224 | 2.79e-53 | 172 |
| MsG0280010891.01.T01 | MTR_2g093980 | 46.269 | 201 | 99 | 3 | 1 | 201 | 87 | 278 | 5.31e-52 | 169 |
| MsG0280010891.01.T01 | MTR_8g087460 | 60.902 | 133 | 48 | 3 | 1 | 130 | 111 | 242 | 2.51e-48 | 160 |
| MsG0280010891.01.T01 | MTR_8g087470 | 60.902 | 133 | 48 | 3 | 1 | 130 | 111 | 242 | 2.76e-48 | 160 |
| MsG0280010891.01.T01 | MTR_4g059780 | 59.231 | 130 | 53 | 0 | 1 | 130 | 108 | 237 | 1.05e-47 | 158 |
| MsG0280010891.01.T01 | MTR_4g059720 | 50.314 | 159 | 63 | 2 | 1 | 159 | 60 | 202 | 3.48e-45 | 150 |
| MsG0280010891.01.T01 | MTR_2g093990 | 53.846 | 130 | 59 | 1 | 1 | 130 | 59 | 187 | 1.07e-44 | 149 |
| MsG0280010891.01.T01 | MTR_7g101080 | 46.875 | 128 | 66 | 2 | 1 | 127 | 58 | 184 | 6.30e-32 | 116 |
| MsG0280010891.01.T01 | MTR_2g090765 | 37.662 | 154 | 89 | 1 | 19 | 165 | 73 | 226 | 1.28e-24 | 97.4 |
| MsG0280010891.01.T01 | MTR_7g075453 | 37.037 | 135 | 85 | 0 | 21 | 155 | 217 | 351 | 5.43e-17 | 79.0 |
| MsG0280010891.01.T01 | MTR_5g098060 | 33.099 | 142 | 87 | 4 | 21 | 160 | 85 | 220 | 4.03e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010891.01.T01 | AT5G60490 | 50.000 | 130 | 63 | 2 | 1 | 129 | 57 | 185 | 3.32e-36 | 127 |
| MsG0280010891.01.T01 | AT5G03170 | 44.295 | 149 | 74 | 2 | 1 | 148 | 54 | 194 | 9.38e-36 | 126 |
| MsG0280010891.01.T01 | AT5G44130 | 35.165 | 182 | 101 | 3 | 1 | 180 | 54 | 220 | 5.63e-25 | 98.2 |
| MsG0280010891.01.T01 | AT2G20520 | 40.000 | 150 | 82 | 4 | 1 | 147 | 56 | 200 | 3.68e-22 | 90.9 |
| MsG0280010891.01.T01 | AT1G03870 | 34.637 | 179 | 106 | 4 | 1 | 173 | 57 | 230 | 4.88e-22 | 90.5 |
| MsG0280010891.01.T01 | AT3G46550 | 33.333 | 120 | 75 | 4 | 18 | 132 | 239 | 358 | 2.06e-11 | 62.8 |
Find 47 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCCAGCAAGTGTTCTTAC+AGG | 0.276561 | 2:+76456914 | None:intergenic |
| ATAAATAGCAAGATGTTTAT+CGG | 0.320043 | 2:+76456793 | None:intergenic |
| GAAAATTGTTGCTGAGGTTC+CGG | 0.329669 | 2:-76456726 | MsG0280010891.01.T01:CDS |
| TGTTGCTGAGGTTCCGGCTT+TGG | 0.331915 | 2:-76456720 | MsG0280010891.01.T01:CDS |
| AGTGTTAACACCACGATTAA+TGG | 0.347939 | 2:-76456827 | MsG0280010891.01.T01:CDS |
| CATCTTGCTATTTATAAAGT+TGG | 0.349855 | 2:-76456785 | MsG0280010891.01.T01:CDS |
| AACCCTGTAAGAACACTTGC+TGG | 0.390538 | 2:-76456917 | MsG0280010891.01.T01:CDS |
| GAACTTAACGTGATTAGTTA+TGG | 0.403328 | 2:-76456875 | MsG0280010891.01.T01:CDS |
| ACTTGGGTGGCACTTGTTGT+TGG | 0.450548 | 2:-76456563 | MsG0280010891.01.T01:CDS |
| TTCTGAGAAGGCGGAATCGT+CGG | 0.454414 | 2:+76457048 | None:intergenic |
| TCTGAGAAGGCGGAATCGTC+GGG | 0.465451 | 2:+76457049 | None:intergenic |
| GCTGGTGCAGGCGCCAAAGC+CGG | 0.482022 | 2:+76456707 | None:intergenic |
| GCCTTAGCATCAGCAATAGC+TGG | 0.484689 | 2:+76456689 | None:intergenic |
| GGAAATGTGAACATTTCAAC+GGG | 0.489369 | 2:-76456851 | MsG0280010891.01.T01:CDS |
| ACCAGCTATTGCTGATGCTA+AGG | 0.493657 | 2:-76456690 | MsG0280010891.01.T01:CDS |
| TTAGGAGCTGTGAGTTGAGC+TGG | 0.506453 | 2:+76457104 | None:intergenic |
| GCCTTCTCAGAACTCAAACC+AGG | 0.511146 | 2:-76457037 | MsG0280010891.01.T01:CDS |
| AGCAAGTGTTCTTACAGGGT+TGG | 0.512112 | 2:+76456919 | None:intergenic |
| GTTGAATCTTCAGATGATGA+TGG | 0.519092 | 2:+76456644 | None:intergenic |
| GGATTGACATGAGTGGAACT+TGG | 0.521312 | 2:-76456580 | MsG0280010891.01.T01:CDS |
| GCATCAGCAATAGCTGGTGC+AGG | 0.524825 | 2:+76456695 | None:intergenic |
| TGCTAAGAAAATTGTTGCTG+AGG | 0.530469 | 2:-76456732 | MsG0280010891.01.T01:CDS |
| ACACTTGCTGGAAACAAACC+TGG | 0.537319 | 2:-76456905 | MsG0280010891.01.T01:CDS |
| GTTGTTGGAATTGTCTTCGT+GGG | 0.546805 | 2:-76456548 | MsG0280010891.01.T01:CDS |
| TTAGCAGGAGAAAATTCTGA+TGG | 0.547442 | 2:+76456749 | None:intergenic |
| GAGAGTGAGTTGAGGAACCC+TGG | 0.549587 | 2:+76457019 | None:intergenic |
| TGCGAGGATCGTTAAGCCGC+CGG | 0.550833 | 2:+76457072 | None:intergenic |
| CAGCTCCTAACCATAAAATC+CGG | 0.551779 | 2:-76457091 | MsG0280010891.01.T01:CDS |
| TTCCAGCAAGTGTTCTTACA+GGG | 0.554792 | 2:+76456915 | None:intergenic |
| TGAAGATTCAACAACGTCGC+AGG | 0.554836 | 2:-76456633 | MsG0280010891.01.T01:CDS |
| CCCTGGTTTGAGTTCTGAGA+AGG | 0.557457 | 2:+76457036 | None:intergenic |
| TGTTGTTGGAATTGTCTTCG+TGG | 0.560250 | 2:-76456549 | MsG0280010891.01.T01:CDS |
| CTTAACGTGATTAGTTATGG+TGG | 0.562819 | 2:-76456872 | MsG0280010891.01.T01:CDS |
| AGGCGGAATCGTCGGGTGCG+AGG | 0.569498 | 2:+76457056 | None:intergenic |
| CTGTGACTTCTAGTGCTATG+AGG | 0.580983 | 2:-76456601 | MsG0280010891.01.T01:CDS |
| CCTTCTCAGAACTCAAACCA+GGG | 0.601582 | 2:-76457036 | MsG0280010891.01.T01:CDS |
| TGGAAATGTGAACATTTCAA+CGG | 0.610164 | 2:-76456852 | MsG0280010891.01.T01:CDS |
| TTTCATTGGAGAGTGAGTTG+AGG | 0.614496 | 2:+76457011 | None:intergenic |
| GTTGGAATTGTCTTCGTGGG+AGG | 0.620149 | 2:-76456545 | MsG0280010891.01.T01:CDS |
| TGGTTTGAGTTCTGAGAAGG+CGG | 0.623094 | 2:+76457039 | None:intergenic |
| GATTGACATGAGTGGAACTT+GGG | 0.631120 | 2:-76456579 | MsG0280010891.01.T01:CDS |
| ATACAAAATCAGAAATAACA+TGG | 0.635906 | 2:+76456966 | None:intergenic |
| ATAGCACTAGAAGTCACAGC+AGG | 0.654697 | 2:+76456605 | None:intergenic |
| ATATATGATTCCATTAATCG+TGG | 0.660112 | 2:+76456817 | None:intergenic |
| CTCCTAACCATAAAATCCGG+CGG | 0.662023 | 2:-76457088 | MsG0280010891.01.T01:CDS |
| TGACATGAGTGGAACTTGGG+TGG | 0.707155 | 2:-76456576 | MsG0280010891.01.T01:CDS |
| GCTATGAGGATTGACATGAG+TGG | 0.723158 | 2:-76456587 | MsG0280010891.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATAGCAAGATGTTTAT+CGG | + | Chr2:76456863-76456882 | None:intergenic | 20.0% |
| !! | ATACAAAATCAGAAATAACA+TGG | + | Chr2:76456690-76456709 | None:intergenic | 20.0% |
| !!! | TAGTTCAAGTTTTTTTTCAT+TGG | + | Chr2:76456659-76456678 | None:intergenic | 20.0% |
| ! | ATATATGATTCCATTAATCG+TGG | + | Chr2:76456839-76456858 | None:intergenic | 25.0% |
| !!! | CATCTTGCTATTTATAAAGT+TGG | - | Chr2:76456868-76456887 | MsG0280010891.01.T01:CDS | 25.0% |
| TGGAAATGTGAACATTTCAA+CGG | - | Chr2:76456801-76456820 | MsG0280010891.01.T01:CDS | 30.0% | |
| ! | GAACTTAACGTGATTAGTTA+TGG | - | Chr2:76456778-76456797 | MsG0280010891.01.T01:CDS | 30.0% |
| !! | GATGATGGTTTTTCTTTTTC+AGG | + | Chr2:76456997-76457016 | None:intergenic | 30.0% |
| AGTGTTAACACCACGATTAA+TGG | - | Chr2:76456826-76456845 | MsG0280010891.01.T01:CDS | 35.0% | |
| GGAAATGTGAACATTTCAAC+GGG | - | Chr2:76456802-76456821 | MsG0280010891.01.T01:CDS | 35.0% | |
| GTTGAATCTTCAGATGATGA+TGG | + | Chr2:76457012-76457031 | None:intergenic | 35.0% | |
| TGCTAAGAAAATTGTTGCTG+AGG | - | Chr2:76456921-76456940 | MsG0280010891.01.T01:CDS | 35.0% | |
| TTAGCAGGAGAAAATTCTGA+TGG | + | Chr2:76456907-76456926 | None:intergenic | 35.0% | |
| ! | ACGTTAAGTTCAACTTTTCC+AGG | + | Chr2:76456769-76456788 | None:intergenic | 35.0% |
| ! | CTTAACGTGATTAGTTATGG+TGG | - | Chr2:76456781-76456800 | MsG0280010891.01.T01:CDS | 35.0% |
| ! | TCAGCAACAATTTTCTTAGC+AGG | + | Chr2:76456922-76456941 | None:intergenic | 35.0% |
| CAGCTCCTAACCATAAAATC+CGG | - | Chr2:76456562-76456581 | MsG0280010891.01.T01:CDS | 40.0% | |
| GAAAATTGTTGCTGAGGTTC+CGG | - | Chr2:76456927-76456946 | MsG0280010891.01.T01:CDS | 40.0% | |
| GATTGACATGAGTGGAACTT+GGG | - | Chr2:76457074-76457093 | MsG0280010891.01.T01:CDS | 40.0% | |
| GTTGTTGGAATTGTCTTCGT+GGG | - | Chr2:76457105-76457124 | MsG0280010891.01.T01:CDS | 40.0% | |
| TGTTGTTGGAATTGTCTTCG+TGG | - | Chr2:76457104-76457123 | MsG0280010891.01.T01:CDS | 40.0% | |
| TTCCAGCAAGTGTTCTTACA+GGG | + | Chr2:76456741-76456760 | None:intergenic | 40.0% | |
| TTTCATTGGAGAGTGAGTTG+AGG | + | Chr2:76456645-76456664 | None:intergenic | 40.0% | |
| TTTCCAGCAAGTGTTCTTAC+AGG | + | Chr2:76456742-76456761 | None:intergenic | 40.0% | |
| !!! | GGTTTTTCTTTTTCAGGCGT+CGG | + | Chr2:76456991-76457010 | None:intergenic | 40.0% |
| AACCCTGTAAGAACACTTGC+TGG | - | Chr2:76456736-76456755 | MsG0280010891.01.T01:CDS | 45.0% | |
| ACCAGCTATTGCTGATGCTA+AGG | - | Chr2:76456963-76456982 | MsG0280010891.01.T01:CDS | 45.0% | |
| AGCAAGTGTTCTTACAGGGT+TGG | + | Chr2:76456737-76456756 | None:intergenic | 45.0% | |
| ATAGCACTAGAAGTCACAGC+AGG | + | Chr2:76457051-76457070 | None:intergenic | 45.0% | |
| CCTTCTCAGAACTCAAACCA+GGG | - | Chr2:76456617-76456636 | MsG0280010891.01.T01:CDS | 45.0% | |
| CTCCTAACCATAAAATCCGG+CGG | - | Chr2:76456565-76456584 | MsG0280010891.01.T01:CDS | 45.0% | |
| CTGTGACTTCTAGTGCTATG+AGG | - | Chr2:76457052-76457071 | MsG0280010891.01.T01:CDS | 45.0% | |
| GCTATGAGGATTGACATGAG+TGG | - | Chr2:76457066-76457085 | MsG0280010891.01.T01:CDS | 45.0% | |
| GGATTGACATGAGTGGAACT+TGG | - | Chr2:76457073-76457092 | MsG0280010891.01.T01:CDS | 45.0% | |
| TGAAGATTCAACAACGTCGC+AGG | - | Chr2:76457020-76457039 | MsG0280010891.01.T01:CDS | 45.0% | |
| ! | ACACTTGCTGGAAACAAACC+TGG | - | Chr2:76456748-76456767 | MsG0280010891.01.T01:CDS | 45.0% |
| !! | TGGTTTGAGTTCTGAGAAGG+CGG | + | Chr2:76456617-76456636 | None:intergenic | 45.0% |
| GCCTTAGCATCAGCAATAGC+TGG | + | Chr2:76456967-76456986 | None:intergenic | 50.0% | |
| GCCTTCTCAGAACTCAAACC+AGG | - | Chr2:76456616-76456635 | MsG0280010891.01.T01:CDS | 50.0% | |
| GTTGGAATTGTCTTCGTGGG+AGG | - | Chr2:76457108-76457127 | MsG0280010891.01.T01:CDS | 50.0% | |
| TGACATGAGTGGAACTTGGG+TGG | - | Chr2:76457077-76457096 | MsG0280010891.01.T01:CDS | 50.0% | |
| ! | AGCCGCCGGATTTTATGGTT+AGG | + | Chr2:76456570-76456589 | None:intergenic | 50.0% |
| ! | CGTTAAGCCGCCGGATTTTA+TGG | + | Chr2:76456575-76456594 | None:intergenic | 50.0% |
| ! | TTAGGAGCTGTGAGTTGAGC+TGG | + | Chr2:76456552-76456571 | None:intergenic | 50.0% |
| ! | TTCTGAGAAGGCGGAATCGT+CGG | + | Chr2:76456608-76456627 | None:intergenic | 50.0% |
| !! | ACTTGGGTGGCACTTGTTGT+TGG | - | Chr2:76457090-76457109 | MsG0280010891.01.T01:CDS | 50.0% |
| !! | CCCTGGTTTGAGTTCTGAGA+AGG | + | Chr2:76456620-76456639 | None:intergenic | 50.0% |
| GCATCAGCAATAGCTGGTGC+AGG | + | Chr2:76456961-76456980 | None:intergenic | 55.0% | |
| TCTGAGAAGGCGGAATCGTC+GGG | + | Chr2:76456607-76456626 | None:intergenic | 55.0% | |
| TGTTGCTGAGGTTCCGGCTT+TGG | - | Chr2:76456933-76456952 | MsG0280010891.01.T01:CDS | 55.0% | |
| ! | GAGAGTGAGTTGAGGAACCC+TGG | + | Chr2:76456637-76456656 | None:intergenic | 55.0% |
| TGCGAGGATCGTTAAGCCGC+CGG | + | Chr2:76456584-76456603 | None:intergenic | 60.0% | |
| AGGCGGAATCGTCGGGTGCG+AGG | + | Chr2:76456600-76456619 | None:intergenic | 70.0% | |
| GCTGGTGCAGGCGCCAAAGC+CGG | + | Chr2:76456949-76456968 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 76456526 | 76457149 | 76456526 | ID=MsG0280010891.01;Name=MsG0280010891.01 |
| Chr2 | mRNA | 76456526 | 76457149 | 76456526 | ID=MsG0280010891.01.T01;Parent=MsG0280010891.01;Name=MsG0280010891.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|207 |
| Chr2 | exon | 76456526 | 76457149 | 76456526 | ID=MsG0280010891.01.T01:exon:8132;Parent=MsG0280010891.01.T01 |
| Chr2 | CDS | 76456526 | 76457149 | 76456526 | ID=MsG0280010891.01.T01:cds;Parent=MsG0280010891.01.T01 |
| Gene Sequence |
| Protein sequence |
