Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023114.01.T01 | AFK42824.1 | 98.246 | 57 | 1 | 0 | 1 | 57 | 4 | 60 | 1.66E-25 | 99.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023114.01.T01 | I3SRD2 | 98.246 | 57 | 1 | 0 | 1 | 57 | 4 | 60 | 7.95e-26 | 99.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010891.01 | MsG0480023114.01 | 0.897539 | 1.274988e-76 | 1.447104e-72 |
| MsG0280010892.01 | MsG0480023114.01 | 0.861106 | 1.295093e-63 | 3.622552e-60 |
| MsG0280010893.01 | MsG0480023114.01 | 0.902704 | 7.383157e-79 | 1.058918e-74 |
| MsG0480020552.01 | MsG0480023114.01 | 0.910300 | 2.185688e-82 | 4.434188e-78 |
| MsG0480020815.01 | MsG0480023114.01 | 0.931343 | 4.347000e-94 | 2.726712e-89 |
| MsG0480020816.01 | MsG0480023114.01 | 0.863646 | 2.145448e-64 | 6.551765e-61 |
| MsG0480020817.01 | MsG0480023114.01 | 0.936159 | 2.710376e-97 | 2.273958e-92 |
| MsG0480020818.01 | MsG0480023114.01 | 0.904232 | 1.521019e-79 | 2.330998e-75 |
| MsG0480023097.01 | MsG0480023114.01 | 0.808287 | 3.335251e-50 | 1.899545e-47 |
| MsG0480023113.01 | MsG0480023114.01 | 0.944638 | 1.348639e-103 | 1.954471e-98 |
| MsG0480023114.01 | MsG0480023115.01 | 0.900335 | 8.118731e-78 | 1.045329e-73 |
| MsG0480023114.01 | MsG0480023116.01 | 0.927619 | 9.131093e-92 | 4.601266e-87 |
| MsG0480023114.01 | MsG0780040592.01 | 0.905853 | 2.765182e-80 | 4.560410e-76 |
| MsG0480023114.01 | MsG0880042557.01 | 0.842044 | 3.288664e-58 | 4.924460e-55 |
| MsG0480023114.01 | MsG0880044994.01 | 0.858715 | 6.808342e-63 | 1.753930e-59 |
| MsG0480023114.01 | MsG0880045339.01 | 0.929064 | 1.187359e-92 | 6.494040e-88 |
| MsG0480023114.01 | MsG0880047773.01 | 0.844166 | 8.947148e-59 | 1.432171e-55 |
| MsG0280006934.01 | MsG0480023114.01 | 0.810433 | 1.155364e-50 | 6.963246e-48 |
| MsG0280009327.01 | MsG0480023114.01 | 0.878148 | 3.549626e-69 | 1.833103e-65 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGTCAATGGTGGGTCCTTT+TGG | 0.235120 | 4:-81090448 | MsG0480023114.01.T01:CDS |
| GGTGCTGTTGCTGTTGTTGC+TGG | 0.314474 | 4:-81090352 | MsG0480023114.01.T01:CDS |
| GTTGCTGTTGTTGCTGGTAA+TGG | 0.333085 | 4:-81090346 | MsG0480023114.01.T01:CDS |
| GGGTGGAATTGGTGAGAGTT+GGG | 0.354472 | 4:-81090272 | MsG0480023114.01.T01:CDS |
| GGGGTGGAATTGGTGAGAGT+TGG | 0.379400 | 4:-81090273 | MsG0480023114.01.T01:CDS |
| GGTGCACCAGTTGGTGTTGT+TGG | 0.431617 | 4:-81090406 | MsG0480023114.01.T01:CDS |
| GGTGATGGAGATGATGTTGT+AGG | 0.435283 | 4:-81090295 | MsG0480023114.01.T01:CDS |
| GGGACTGTTATGGTGGGTGA+TGG | 0.441052 | 4:-81090427 | MsG0480023114.01.T01:CDS |
| GGTGGTGATGCTGTTGTTGA+TGG | 0.445870 | 4:-81090325 | MsG0480023114.01.T01:CDS |
| TGATGCTGTTGTTGATGGTA+AGG | 0.449757 | 4:-81090320 | MsG0480023114.01.T01:CDS |
| AAGAATGTTGATTATGTCAA+TGG | 0.455371 | 4:-81090461 | None:intergenic |
| GTGATGGAGATGATGTTGTA+GGG | 0.461192 | 4:-81090294 | MsG0480023114.01.T01:CDS |
| CTTGTTCCAACAACACCAAC+TGG | 0.476196 | 4:+81090400 | None:intergenic |
| TGGTGAGAGTTGGGCTAAAG+TGG | 0.489699 | 4:-81090263 | MsG0480023114.01.T01:CDS |
| AGTGGAACTGTGCTTAATCC+TGG | 0.544466 | 4:-81090379 | MsG0480023114.01.T01:CDS |
| TGATGGAGATGATGTTGTAG+GGG | 0.546507 | 4:-81090293 | MsG0480023114.01.T01:CDS |
| GTTGGTGTTGTTGGAACAAG+TGG | 0.555566 | 4:-81090397 | MsG0480023114.01.T01:CDS |
| GTTGATGGTAAGGGTGGTGA+TGG | 0.560733 | 4:-81090310 | MsG0480023114.01.T01:CDS |
| CCCACCATAACAGTCCCAAA+AGG | 0.562203 | 4:+81090433 | None:intergenic |
| GTGGGTGATGGTGCACCAGT+TGG | 0.574265 | 4:-81090415 | MsG0480023114.01.T01:CDS |
| GCTGTTGTTGATGGTAAGGG+TGG | 0.581658 | 4:-81090316 | MsG0480023114.01.T01:CDS |
| GCTGTTGTTGCTGGTAATGG+TGG | 0.582499 | 4:-81090343 | MsG0480023114.01.T01:CDS |
| GATGGAGATGATGTTGTAGG+GGG | 0.584616 | 4:-81090292 | MsG0480023114.01.T01:CDS |
| GAGTTGGGCTAAAGTGGTGG+AGG | 0.587512 | 4:-81090257 | MsG0480023114.01.T01:CDS |
| TGAGAGTTGGGCTAAAGTGG+TGG | 0.590350 | 4:-81090260 | MsG0480023114.01.T01:CDS |
| ACAGCAACAGCACCATCACC+AGG | 0.599988 | 4:+81090361 | None:intergenic |
| ACTGTGCTTAATCCTGGTGA+TGG | 0.604248 | 4:-81090373 | MsG0480023114.01.T01:CDS |
| GATGCTGTTGTTGATGGTAA+GGG | 0.606751 | 4:-81090319 | MsG0480023114.01.T01:CDS |
| AATGTTGATTATGTCAATGG+TGG | 0.616748 | 4:-81090458 | None:intergenic |
| ATGTTGATTATGTCAATGGT+GGG | 0.681774 | 4:-81090457 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGATGGAGATGATGTTGTAG+GGG | - | Chr4:81090407-81090426 | MsG0480023114.01.T01:CDS | 40.0% | |
| ! | GTGATGGAGATGATGTTGTA+GGG | - | Chr4:81090406-81090425 | MsG0480023114.01.T01:CDS | 40.0% |
| !! | GATGCTGTTGTTGATGGTAA+GGG | - | Chr4:81090381-81090400 | MsG0480023114.01.T01:CDS | 40.0% |
| !! | TGATGCTGTTGTTGATGGTA+AGG | - | Chr4:81090380-81090399 | MsG0480023114.01.T01:CDS | 40.0% |
| ACTGTGCTTAATCCTGGTGA+TGG | - | Chr4:81090327-81090346 | MsG0480023114.01.T01:CDS | 45.0% | |
| AGTGGAACTGTGCTTAATCC+TGG | - | Chr4:81090321-81090340 | MsG0480023114.01.T01:CDS | 45.0% | |
| CTTGTTCCAACAACACCAAC+TGG | + | Chr4:81090303-81090322 | None:intergenic | 45.0% | |
| GATGGAGATGATGTTGTAGG+GGG | - | Chr4:81090408-81090427 | MsG0480023114.01.T01:CDS | 45.0% | |
| ! | GTTGCTGTTGTTGCTGGTAA+TGG | - | Chr4:81090354-81090373 | MsG0480023114.01.T01:CDS | 45.0% |
| !! | GGTGATGGAGATGATGTTGT+AGG | - | Chr4:81090405-81090424 | MsG0480023114.01.T01:CDS | 45.0% |
| !! | GTTGGTGTTGTTGGAACAAG+TGG | - | Chr4:81090303-81090322 | MsG0480023114.01.T01:CDS | 45.0% |
| !!! | ATGTCAATGGTGGGTCCTTT+TGG | - | Chr4:81090252-81090271 | MsG0480023114.01.T01:CDS | 45.0% |
| !!! | CCTTTTGGGACTGTTATGGT+GGG | - | Chr4:81090267-81090286 | MsG0480023114.01.T01:CDS | 45.0% |
| !!! | TCCTTTTGGGACTGTTATGG+TGG | - | Chr4:81090266-81090285 | MsG0480023114.01.T01:CDS | 45.0% |
| !!! | TGTCAATGGTGGGTCCTTTT+GGG | - | Chr4:81090253-81090272 | MsG0480023114.01.T01:CDS | 45.0% |
| CCCACCATAACAGTCCCAAA+AGG | + | Chr4:81090270-81090289 | None:intergenic | 50.0% | |
| GATGTTGTAGGGGGTGGAAT+TGG | - | Chr4:81090417-81090436 | MsG0480023114.01.T01:CDS | 50.0% | |
| GGGTGGAATTGGTGAGAGTT+GGG | - | Chr4:81090428-81090447 | MsG0480023114.01.T01:CDS | 50.0% | |
| TGAGAGTTGGGCTAAAGTGG+TGG | - | Chr4:81090440-81090459 | MsG0480023114.01.T01:CDS | 50.0% | |
| TGGTGAGAGTTGGGCTAAAG+TGG | - | Chr4:81090437-81090456 | MsG0480023114.01.T01:CDS | 50.0% | |
| ! | GGTGGTGATGCTGTTGTTGA+TGG | - | Chr4:81090375-81090394 | MsG0480023114.01.T01:CDS | 50.0% |
| !! | GCTGTTGTTGATGGTAAGGG+TGG | - | Chr4:81090384-81090403 | MsG0480023114.01.T01:CDS | 50.0% |
| !! | GCTGTTGTTGCTGGTAATGG+TGG | - | Chr4:81090357-81090376 | MsG0480023114.01.T01:CDS | 50.0% |
| !! | GTTGATGGTAAGGGTGGTGA+TGG | - | Chr4:81090390-81090409 | MsG0480023114.01.T01:CDS | 50.0% |
| !!! | GGGTCCTTTTGGGACTGTTA+TGG | - | Chr4:81090263-81090282 | MsG0480023114.01.T01:CDS | 50.0% |
| ACAGCAACAGCACCATCACC+AGG | + | Chr4:81090342-81090361 | None:intergenic | 55.0% | |
| GAGTTGGGCTAAAGTGGTGG+AGG | - | Chr4:81090443-81090462 | MsG0480023114.01.T01:CDS | 55.0% | |
| GGAGATGATGTTGTAGGGGG+TGG | - | Chr4:81090411-81090430 | MsG0480023114.01.T01:CDS | 55.0% | |
| GGGGTGGAATTGGTGAGAGT+TGG | - | Chr4:81090427-81090446 | MsG0480023114.01.T01:CDS | 55.0% | |
| !! | GGGACTGTTATGGTGGGTGA+TGG | - | Chr4:81090273-81090292 | MsG0480023114.01.T01:CDS | 55.0% |
| !! | GGTGCACCAGTTGGTGTTGT+TGG | - | Chr4:81090294-81090313 | MsG0480023114.01.T01:CDS | 55.0% |
| !! | GGTGCTGTTGCTGTTGTTGC+TGG | - | Chr4:81090348-81090367 | MsG0480023114.01.T01:CDS | 55.0% |
| ! | GTGGGTGATGGTGCACCAGT+TGG | - | Chr4:81090285-81090304 | MsG0480023114.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 81090252 | 81090470 | 81090252 | ID=MsG0480023114.01;Name=MsG0480023114.01 |
| Chr4 | mRNA | 81090252 | 81090470 | 81090252 | ID=MsG0480023114.01.T01;Parent=MsG0480023114.01;Name=MsG0480023114.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|72 |
| Chr4 | exon | 81090252 | 81090470 | 81090252 | ID=MsG0480023114.01.T01:exon:2359;Parent=MsG0480023114.01.T01 |
| Chr4 | CDS | 81090252 | 81090470 | 81090252 | ID=MsG0480023114.01.T01:cds;Parent=MsG0480023114.01.T01 |
| Gene Sequence |
| Protein sequence |
