Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010893.01.T01 | MCI15070.1 | 84.211 | 76 | 8 | 2 | 1 | 73 | 73 | 147 | 7.49E-23 | 95.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280010893.01.T01 | A0A392PUE1 | 84.211 | 76 | 8 | 2 | 1 | 73 | 73 | 147 | 3.58e-23 | 95.9 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000319.01 | MsG0280010893.01 | 0.821047 | 4.970275e-53 | 4.000286e-50 |
| MsG0280010891.01 | MsG0280010893.01 | 0.821389 | 4.145144e-53 | 3.367707e-50 |
| MsG0280010892.01 | MsG0280010893.01 | 0.918697 | 1.131693e-86 | 3.493739e-82 |
| MsG0280010893.01 | MsG0480020552.01 | 0.827864 | 1.236102e-54 | 1.208282e-51 |
| MsG0280010893.01 | MsG0480020815.01 | 0.927829 | 6.802088e-92 | 3.469833e-87 |
| MsG0280010893.01 | MsG0480020816.01 | 0.846916 | 1.607542e-59 | 2.809813e-56 |
| MsG0280010893.01 | MsG0480020817.01 | 0.865328 | 6.397202e-65 | 2.073072e-61 |
| MsG0280010893.01 | MsG0480020818.01 | 0.823156 | 1.612079e-53 | 1.377042e-50 |
| MsG0280010893.01 | MsG0480023097.01 | 0.863125 | 3.111582e-64 | 9.331216e-61 |
| MsG0280010893.01 | MsG0480023113.01 | 0.946277 | 6.267086e-105 | 1.017320e-99 |
| MsG0280010893.01 | MsG0480023114.01 | 0.902704 | 7.383157e-79 | 1.058918e-74 |
| MsG0280010893.01 | MsG0480023116.01 | 0.877806 | 4.675696e-69 | 2.383434e-65 |
| MsG0280010893.01 | MsG0580025594.01 | 0.819407 | 1.180177e-52 | 9.073874e-50 |
| MsG0280010893.01 | MsG0780040592.01 | 0.871054 | 9.149287e-67 | 3.639604e-63 |
| MsG0280010893.01 | MsG0880044994.01 | 0.885675 | 6.628343e-72 | 4.591579e-68 |
| MsG0280010893.01 | MsG0880045339.01 | 0.867061 | 1.806690e-65 | 6.223618e-62 |
| MsG0280009327.01 | MsG0280010893.01 | 0.886906 | 2.275073e-72 | 1.656198e-68 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGAGAAGGAAAAGATAATT+TGG | 0.276779 | 2:+76461450 | MsG0280010893.01.T01:CDS |
| AAGATAATTTGGTTCATGTT+TGG | 0.338084 | 2:+76461461 | MsG0280010893.01.T01:CDS |
| TCTGGTAATGTTGGGACTAA+TGG | 0.353632 | 2:+76461308 | MsG0280010893.01.T01:CDS |
| GTTGCTGTTGCTGCCGTGTT+GGG | 0.357964 | 2:+76461395 | MsG0280010893.01.T01:CDS |
| TTTGGTGCCGGGGCATGTTT+CGG | 0.395055 | 2:+76461356 | MsG0280010893.01.T01:CDS |
| GATGTTGGTGATGCTGCTTT+TGG | 0.401270 | 2:+76461338 | MsG0280010893.01.T01:CDS |
| TGTTGCTGTTGCTGCCGTGT+TGG | 0.415192 | 2:+76461394 | MsG0280010893.01.T01:CDS |
| GCATGTTTCGGTGACGCTGC+TGG | 0.495698 | 2:+76461368 | MsG0280010893.01.T01:CDS |
| TCTGAGGTGTCTGGTAATGT+TGG | 0.510374 | 2:+76461299 | MsG0280010893.01.T01:CDS |
| CTGAGGTGTCTGGTAATGTT+GGG | 0.532113 | 2:+76461300 | MsG0280010893.01.T01:CDS |
| GTCGTCCGGGGTGGAATCTG+AGG | 0.559966 | 2:+76461283 | MsG0280010893.01.T01:CDS |
| CATGAGTAGAAAGAGTGAGA+AGG | 0.564185 | 2:+76461436 | MsG0280010893.01.T01:CDS |
| GGGGTGGAATCTGAGGTGTC+TGG | 0.574659 | 2:+76461290 | MsG0280010893.01.T01:CDS |
| AGCGTCACCGAAACATGCCC+CGG | 0.581778 | 2:-76461363 | None:intergenic |
| CTCATTGTATTCTCCCAACA+CGG | 0.596372 | 2:-76461408 | None:intergenic |
| GGCTTGATAATTGAAGATTG+TGG | 0.606110 | 2:+76461482 | MsG0280010893.01.T01:CDS |
| TTGATAATTGAAGATTGTGG+AGG | 0.654152 | 2:+76461485 | MsG0280010893.01.T01:CDS |
| CGTGTTGGGAGAATACAATG+AGG | 0.742075 | 2:+76461409 | MsG0280010893.01.T01:CDS |
| AGACACCTCAGATTCCACCC+CGG | 0.745070 | 2:-76461288 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGATAATTTGGTTCATGTT+TGG | + | Chr2:76461461-76461480 | MsG0280010893.01.T01:CDS | 25.0% |
| GTGAGAAGGAAAAGATAATT+TGG | + | Chr2:76461450-76461469 | MsG0280010893.01.T01:CDS | 30.0% | |
| ! | TTGATAATTGAAGATTGTGG+AGG | + | Chr2:76461485-76461504 | MsG0280010893.01.T01:CDS | 30.0% |
| ! | GGCTTGATAATTGAAGATTG+TGG | + | Chr2:76461482-76461501 | MsG0280010893.01.T01:CDS | 35.0% |
| !!! | ACTAATGGTTTTGTCGATGT+TGG | + | Chr2:76461323-76461342 | MsG0280010893.01.T01:CDS | 35.0% |
| CATGAGTAGAAAGAGTGAGA+AGG | + | Chr2:76461436-76461455 | MsG0280010893.01.T01:CDS | 40.0% | |
| CTCATTGTATTCTCCCAACA+CGG | - | Chr2:76461411-76461430 | None:intergenic | 40.0% | |
| ! | TCTGGTAATGTTGGGACTAA+TGG | + | Chr2:76461308-76461327 | MsG0280010893.01.T01:CDS | 40.0% |
| CGTGTTGGGAGAATACAATG+AGG | + | Chr2:76461409-76461428 | MsG0280010893.01.T01:CDS | 45.0% | |
| ! | CTGAGGTGTCTGGTAATGTT+GGG | + | Chr2:76461300-76461319 | MsG0280010893.01.T01:CDS | 45.0% |
| ! | TCTGAGGTGTCTGGTAATGT+TGG | + | Chr2:76461299-76461318 | MsG0280010893.01.T01:CDS | 45.0% |
| !!! | GATGTTGGTGATGCTGCTTT+TGG | + | Chr2:76461338-76461357 | MsG0280010893.01.T01:CDS | 45.0% |
| AGACACCTCAGATTCCACCC+CGG | - | Chr2:76461291-76461310 | None:intergenic | 55.0% | |
| ! | GTTGCTGTTGCTGCCGTGTT+GGG | + | Chr2:76461395-76461414 | MsG0280010893.01.T01:CDS | 55.0% |
| ! | TGTTGCTGTTGCTGCCGTGT+TGG | + | Chr2:76461394-76461413 | MsG0280010893.01.T01:CDS | 55.0% |
| !! | TTTGGTGCCGGGGCATGTTT+CGG | + | Chr2:76461356-76461375 | MsG0280010893.01.T01:CDS | 55.0% |
| !!! | GGTGATGCTGCTTTTGGTGC+CGG | + | Chr2:76461344-76461363 | MsG0280010893.01.T01:CDS | 55.0% |
| !!! | GTGATGCTGCTTTTGGTGCC+GGG | + | Chr2:76461345-76461364 | MsG0280010893.01.T01:CDS | 55.0% |
| !!! | TGATGCTGCTTTTGGTGCCG+GGG | + | Chr2:76461346-76461365 | MsG0280010893.01.T01:CDS | 55.0% |
| AGCGTCACCGAAACATGCCC+CGG | - | Chr2:76461366-76461385 | None:intergenic | 60.0% | |
| GCATGTTTCGGTGACGCTGC+TGG | + | Chr2:76461368-76461387 | MsG0280010893.01.T01:CDS | 60.0% | |
| ! | GGGGTGGAATCTGAGGTGTC+TGG | + | Chr2:76461290-76461309 | MsG0280010893.01.T01:CDS | 60.0% |
| GTCGTCCGGGGTGGAATCTG+AGG | + | Chr2:76461283-76461302 | MsG0280010893.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 76461281 | 76461514 | 76461281 | ID=MsG0280010893.01;Name=MsG0280010893.01 |
| Chr2 | mRNA | 76461281 | 76461514 | 76461281 | ID=MsG0280010893.01.T01;Parent=MsG0280010893.01;Name=MsG0280010893.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|77 |
| Chr2 | exon | 76461281 | 76461514 | 76461281 | ID=MsG0280010893.01.T01:exon:1897;Parent=MsG0280010893.01.T01 |
| Chr2 | CDS | 76461281 | 76461514 | 76461281 | ID=MsG0280010893.01.T01:cds;Parent=MsG0280010893.01.T01 |
| Gene Sequence |
| Protein sequence |
