Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020552.01.T01 | XP_003606125.1 | 97.525 | 202 | 5 | 0 | 1 | 202 | 95 | 296 | 1.84E-138 | 399 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020552.01.T01 | Q8LEJ6 | 49.664 | 149 | 66 | 3 | 1 | 146 | 54 | 196 | 1.34E-39 | 138 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020552.01.T01 | G7JIF3 | 97.525 | 202 | 5 | 0 | 1 | 202 | 95 | 296 | 8.77e-139 | 399 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010891.01 | MsG0480020552.01 | 0.880146 | 6.981598e-70 | 3.890044e-66 |
| MsG0280010892.01 | MsG0480020552.01 | 0.838703 | 2.456691e-57 | 3.317395e-54 |
| MsG0280010893.01 | MsG0480020552.01 | 0.827864 | 1.236102e-54 | 1.208282e-51 |
| MsG0480020552.01 | MsG0480020815.01 | 0.887775 | 1.061219e-72 | 8.002375e-69 |
| MsG0480020552.01 | MsG0480020816.01 | 0.878165 | 3.500035e-69 | 1.808826e-65 |
| MsG0480020552.01 | MsG0480020817.01 | 0.918989 | 7.873870e-87 | 2.469379e-82 |
| MsG0480020552.01 | MsG0480020818.01 | 0.948026 | 2.130054e-106 | 3.867706e-101 |
| MsG0480020552.01 | MsG0480023113.01 | 0.891095 | 5.431579e-74 | 4.695972e-70 |
| MsG0480020552.01 | MsG0480023114.01 | 0.910300 | 2.185688e-82 | 4.434188e-78 |
| MsG0480020552.01 | MsG0480023115.01 | 0.916247 | 2.240616e-85 | 6.107834e-81 |
| MsG0480020552.01 | MsG0480023116.01 | 0.941738 | 2.461833e-101 | 2.945999e-96 |
| MsG0480020552.01 | MsG0780040592.01 | 0.840869 | 6.705949e-58 | 9.680690e-55 |
| MsG0480020552.01 | MsG0880042557.01 | 0.868189 | 7.856647e-66 | 2.816788e-62 |
| MsG0480020552.01 | MsG0880044994.01 | 0.844995 | 5.349961e-59 | 8.790412e-56 |
| MsG0480020552.01 | MsG0880045339.01 | 0.919204 | 6.027958e-87 | 1.912616e-82 |
| MsG0480020552.01 | MsG0880047773.01 | 0.921980 | 1.780413e-88 | 6.554605e-84 |
| MsG0280006934.01 | MsG0480020552.01 | 0.863899 | 1.790769e-64 | 5.516973e-61 |
| MsG0280009327.01 | MsG0480020552.01 | 0.849019 | 4.229922e-60 | 7.911611e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020552.01.T01 | MTR_4g053380 | 97.525 | 202 | 5 | 0 | 1 | 202 | 95 | 296 | 2.22e-142 | 399 |
| MsG0480020552.01.T01 | MTR_4g059790 | 83.854 | 192 | 26 | 3 | 1 | 190 | 91 | 279 | 4.16e-105 | 304 |
| MsG0480020552.01.T01 | MTR_4g059840 | 80.296 | 203 | 33 | 4 | 1 | 199 | 91 | 290 | 5.60e-105 | 304 |
| MsG0480020552.01.T01 | MTR_2g093970 | 65.517 | 203 | 61 | 4 | 1 | 197 | 87 | 286 | 4.34e-80 | 241 |
| MsG0480020552.01.T01 | MTR_2g017970 | 62.025 | 158 | 52 | 2 | 1 | 157 | 86 | 236 | 3.82e-61 | 192 |
| MsG0480020552.01.T01 | MTR_2g017960 | 61.392 | 158 | 53 | 2 | 1 | 157 | 86 | 236 | 6.84e-60 | 189 |
| MsG0480020552.01.T01 | MTR_2g093980 | 48.259 | 201 | 98 | 2 | 1 | 200 | 87 | 282 | 6.72e-57 | 181 |
| MsG0480020552.01.T01 | MTR_8g087450 | 65.385 | 130 | 45 | 0 | 1 | 130 | 95 | 224 | 9.08e-57 | 181 |
| MsG0480020552.01.T01 | MTR_2g017950 | 49.528 | 212 | 81 | 5 | 1 | 197 | 89 | 289 | 3.36e-54 | 175 |
| MsG0480020552.01.T01 | MTR_8g087460 | 63.910 | 133 | 44 | 3 | 1 | 130 | 111 | 242 | 1.85e-51 | 168 |
| MsG0480020552.01.T01 | MTR_8g087470 | 63.910 | 133 | 44 | 3 | 1 | 130 | 111 | 242 | 1.87e-51 | 168 |
| MsG0480020552.01.T01 | MTR_4g059780 | 58.333 | 132 | 55 | 0 | 1 | 132 | 108 | 239 | 4.58e-49 | 162 |
| MsG0480020552.01.T01 | MTR_4g059720 | 60.741 | 135 | 51 | 2 | 1 | 135 | 60 | 192 | 9.85e-49 | 159 |
| MsG0480020552.01.T01 | MTR_2g093990 | 56.489 | 131 | 56 | 1 | 1 | 131 | 59 | 188 | 3.16e-45 | 150 |
| MsG0480020552.01.T01 | MTR_7g101080 | 47.727 | 132 | 67 | 2 | 1 | 131 | 58 | 188 | 1.07e-33 | 121 |
| MsG0480020552.01.T01 | MTR_2g090765 | 39.130 | 161 | 81 | 2 | 19 | 168 | 73 | 227 | 1.17e-25 | 100 |
| MsG0480020552.01.T01 | MTR_7g075453 | 34.043 | 141 | 93 | 0 | 8 | 148 | 204 | 344 | 1.90e-16 | 77.0 |
| MsG0480020552.01.T01 | MTR_5g098060 | 32.195 | 205 | 125 | 6 | 1 | 199 | 66 | 262 | 1.03e-14 | 70.9 |
| MsG0480020552.01.T01 | MTR_2g437700 | 35.652 | 115 | 70 | 3 | 21 | 131 | 230 | 344 | 2.05e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020552.01.T01 | AT5G03170 | 49.664 | 149 | 66 | 3 | 1 | 146 | 54 | 196 | 1.36e-40 | 138 |
| MsG0480020552.01.T01 | AT5G60490 | 53.030 | 132 | 60 | 2 | 1 | 131 | 57 | 187 | 4.80e-39 | 134 |
| MsG0480020552.01.T01 | AT5G44130 | 35.135 | 185 | 108 | 5 | 1 | 182 | 54 | 229 | 8.91e-25 | 97.8 |
| MsG0480020552.01.T01 | AT2G20520 | 39.344 | 183 | 92 | 6 | 1 | 173 | 56 | 229 | 6.72e-24 | 95.5 |
| MsG0480020552.01.T01 | AT1G03870 | 37.748 | 151 | 86 | 4 | 1 | 149 | 57 | 201 | 4.14e-20 | 85.5 |
| MsG0480020552.01.T01 | AT3G46550 | 34.559 | 136 | 79 | 5 | 18 | 143 | 239 | 374 | 4.32e-13 | 67.4 |
| MsG0480020552.01.T01 | AT2G04780 | 33.628 | 113 | 70 | 2 | 21 | 131 | 83 | 192 | 2.01e-11 | 61.6 |
| MsG0480020552.01.T01 | AT2G04780 | 33.628 | 113 | 70 | 2 | 21 | 131 | 83 | 192 | 2.01e-11 | 61.6 |
| MsG0480020552.01.T01 | AT3G60900 | 29.927 | 137 | 91 | 2 | 21 | 156 | 223 | 355 | 7.64e-11 | 60.8 |
Find 48 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACATTCAGTTCCACCTTTC+CGG | 0.138093 | 4:-43258247 | None:intergenic |
| GGCTTCTCCTTATCAGCTTT+TGG | 0.173769 | 4:-43258459 | None:intergenic |
| TCCGAGGAATCTGGCGATAA+TGG | 0.305230 | 4:-43258480 | None:intergenic |
| CCAACTCAATTCACAACTAT+TGG | 0.336498 | 4:+43258029 | MsG0480020552.01.T01:CDS |
| CGTCTTGCTATTTATAAAGT+TGG | 0.340020 | 4:+43258348 | MsG0480020552.01.T01:CDS |
| CAACTATTGGCAACAAAATC+AGG | 0.371289 | 4:+43258042 | MsG0480020552.01.T01:CDS |
| ACCGACGAAGGGACTGTCTT+TGG | 0.372165 | 4:+43258413 | MsG0480020552.01.T01:CDS |
| ACATTCAGTTCCACCTTTCC+GGG | 0.381438 | 4:-43258246 | None:intergenic |
| AACCCTGTGAGAACACTTGC+TGG | 0.387148 | 4:+43258216 | MsG0480020552.01.T01:CDS |
| TGGCAAAGTGCTTCTTCCTA+TGG | 0.388422 | 4:+43258368 | MsG0480020552.01.T01:CDS |
| ACAGATGAATCCGAGGAATC+TGG | 0.415550 | 4:-43258489 | None:intergenic |
| GGAAGTGTGAACATATCAAC+AGG | 0.440559 | 4:+43258282 | MsG0480020552.01.T01:CDS |
| TGTGAGAAGGCGTTGTCATC+CGG | 0.456479 | 4:-43258084 | None:intergenic |
| GCCATTATCGCCAGATTCCT+CGG | 0.463299 | 4:+43258479 | MsG0480020552.01.T01:CDS |
| GGAGTCGTTGTTGAAGGCTC+TGG | 0.469840 | 4:-43258438 | None:intergenic |
| AGTACCAGAGTTATCATTTG+TGG | 0.474963 | 4:-43258517 | None:intergenic |
| AACGACTCCAAAAGCTGATA+AGG | 0.478459 | 4:+43258452 | MsG0480020552.01.T01:CDS |
| ACTGTGAAAATGAATGTGTA+TGG | 0.486591 | 4:+43258537 | MsG0480020552.01.T01:CDS |
| GTACCAGAGTTATCATTTGT+GGG | 0.489167 | 4:-43258516 | None:intergenic |
| CCAATAGTTGTGAATTGAGT+TGG | 0.499317 | 4:-43258029 | None:intergenic |
| GCTCCAGCAAGTGTTCTCAC+AGG | 0.519482 | 4:-43258219 | None:intergenic |
| CTCCAGCAAGTGTTCTCACA+GGG | 0.538486 | 4:-43258218 | None:intergenic |
| TGGGTTGACAATTCTTGCAC+CGG | 0.540458 | 4:+43258065 | MsG0480020552.01.T01:CDS |
| TTCCTCAACTCACTTTCCGA+TGG | 0.541050 | 4:+43258120 | MsG0480020552.01.T01:CDS |
| AAATGAATGTGTATGGAAAG+TGG | 0.552632 | 4:+43258544 | MsG0480020552.01.T01:CDS |
| GCAACAGAAAAGAAATCCAT+AGG | 0.554500 | 4:-43258384 | None:intergenic |
| GCCTTCTCACAACTCAAAGC+AGG | 0.566899 | 4:+43258096 | MsG0480020552.01.T01:CDS |
| CAGCTCAAGTTTCTGTCCAT+CGG | 0.571872 | 4:-43258136 | None:intergenic |
| GAACTGAATGTGATAAGTTA+CGG | 0.572972 | 4:+43258258 | MsG0480020552.01.T01:CDS |
| TCCTGCTTTGAGTTGTGAGA+AGG | 0.576789 | 4:-43258097 | None:intergenic |
| TGCTGGAGCTAAACCCGGAA+AGG | 0.580904 | 4:+43258233 | MsG0480020552.01.T01:CDS |
| AATGAATGTGTATGGAAAGT+GGG | 0.589784 | 4:+43258545 | MsG0480020552.01.T01:CDS |
| TGTTGCTAAGGCACCGACGA+AGG | 0.590444 | 4:+43258401 | MsG0480020552.01.T01:CDS |
| ACACTTGCTGGAGCTAAACC+CGG | 0.593390 | 4:+43258228 | MsG0480020552.01.T01:CDS |
| GCCAAAGACAGTCCCTTCGT+CGG | 0.593435 | 4:-43258414 | None:intergenic |
| GTTGCTAAGGCACCGACGAA+GGG | 0.594871 | 4:+43258402 | MsG0480020552.01.T01:CDS |
| AAGCCCACAAATGATAACTC+TGG | 0.604424 | 4:+43258513 | MsG0480020552.01.T01:CDS |
| AATCACATTCTGATTTATCG+TGG | 0.607000 | 4:-43258606 | None:intergenic |
| GTCCATCGGAAAGTGAGTTG+AGG | 0.623528 | 4:-43258122 | None:intergenic |
| GAAGTGAACACAACGATCGA+TGG | 0.624371 | 4:+43258306 | MsG0480020552.01.T01:CDS |
| ATACGTAATCTGAAATAACA+TGG | 0.631817 | 4:-43258167 | None:intergenic |
| TGGAGCTAAACCCGGAAAGG+TGG | 0.639885 | 4:+43258236 | MsG0480020552.01.T01:CDS |
| CTATTGGCAACAAAATCAGG+TGG | 0.642175 | 4:+43258045 | MsG0480020552.01.T01:CDS |
| TATTGGCAACAAAATCAGGT+GGG | 0.667970 | 4:+43258046 | MsG0480020552.01.T01:CDS |
| CTGAATGTGATAAGTTACGG+TGG | 0.671159 | 4:+43258261 | MsG0480020552.01.T01:CDS |
| GGGCTTAACAGATGAATCCG+AGG | 0.698996 | 4:-43258496 | None:intergenic |
| GAAGTGTGAACATATCAACA+GGG | 0.726270 | 4:+43258283 | MsG0480020552.01.T01:CDS |
| AAGTGTGAACATATCAACAG+GGG | 0.748931 | 4:+43258284 | MsG0480020552.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACGTAATCTGAAATAACA+TGG | - | Chr4:43258170-43258189 | None:intergenic | 25.0% |
| AAATGAATGTGTATGGAAAG+TGG | + | Chr4:43258544-43258563 | MsG0480020552.01.T01:CDS | 30.0% | |
| AATGAATGTGTATGGAAAGT+GGG | + | Chr4:43258545-43258564 | MsG0480020552.01.T01:CDS | 30.0% | |
| ACTGTGAAAATGAATGTGTA+TGG | + | Chr4:43258537-43258556 | MsG0480020552.01.T01:CDS | 30.0% | |
| GAACTGAATGTGATAAGTTA+CGG | + | Chr4:43258258-43258277 | MsG0480020552.01.T01:CDS | 30.0% | |
| !! | CGTCTTGCTATTTATAAAGT+TGG | + | Chr4:43258348-43258367 | MsG0480020552.01.T01:CDS | 30.0% |
| AAGTGTGAACATATCAACAG+GGG | + | Chr4:43258284-43258303 | MsG0480020552.01.T01:CDS | 35.0% | |
| AGTACCAGAGTTATCATTTG+TGG | - | Chr4:43258520-43258539 | None:intergenic | 35.0% | |
| CAACTATTGGCAACAAAATC+AGG | + | Chr4:43258042-43258061 | MsG0480020552.01.T01:CDS | 35.0% | |
| CCAACTCAATTCACAACTAT+TGG | + | Chr4:43258029-43258048 | MsG0480020552.01.T01:CDS | 35.0% | |
| CCAATAGTTGTGAATTGAGT+TGG | - | Chr4:43258032-43258051 | None:intergenic | 35.0% | |
| GAAGTGTGAACATATCAACA+GGG | + | Chr4:43258283-43258302 | MsG0480020552.01.T01:CDS | 35.0% | |
| GCAACAGAAAAGAAATCCAT+AGG | - | Chr4:43258387-43258406 | None:intergenic | 35.0% | |
| GTACCAGAGTTATCATTTGT+GGG | - | Chr4:43258519-43258538 | None:intergenic | 35.0% | |
| TATTGGCAACAAAATCAGGT+GGG | + | Chr4:43258046-43258065 | MsG0480020552.01.T01:CDS | 35.0% | |
| ! | GGATTTCTTTTCTGTTGCTA+AGG | + | Chr4:43258389-43258408 | MsG0480020552.01.T01:CDS | 35.0% |
| AACGACTCCAAAAGCTGATA+AGG | + | Chr4:43258452-43258471 | MsG0480020552.01.T01:CDS | 40.0% | |
| AAGCCCACAAATGATAACTC+TGG | + | Chr4:43258513-43258532 | MsG0480020552.01.T01:CDS | 40.0% | |
| CTATTGGCAACAAAATCAGG+TGG | + | Chr4:43258045-43258064 | MsG0480020552.01.T01:CDS | 40.0% | |
| CTGAATGTGATAAGTTACGG+TGG | + | Chr4:43258261-43258280 | MsG0480020552.01.T01:CDS | 40.0% | |
| ! | GGAAGTGTGAACATATCAAC+AGG | + | Chr4:43258282-43258301 | MsG0480020552.01.T01:CDS | 40.0% |
| ACAGATGAATCCGAGGAATC+TGG | - | Chr4:43258492-43258511 | None:intergenic | 45.0% | |
| ACATTCAGTTCCACCTTTCC+GGG | - | Chr4:43258249-43258268 | None:intergenic | 45.0% | |
| CACATTCAGTTCCACCTTTC+CGG | - | Chr4:43258250-43258269 | None:intergenic | 45.0% | |
| CAGCTCAAGTTTCTGTCCAT+CGG | - | Chr4:43258139-43258158 | None:intergenic | 45.0% | |
| GAAGTGAACACAACGATCGA+TGG | + | Chr4:43258306-43258325 | MsG0480020552.01.T01:CDS | 45.0% | |
| TTCCTCAACTCACTTTCCGA+TGG | + | Chr4:43258120-43258139 | MsG0480020552.01.T01:CDS | 45.0% | |
| ! | GGCTTCTCCTTATCAGCTTT+TGG | - | Chr4:43258462-43258481 | None:intergenic | 45.0% |
| ! | TGGCAAAGTGCTTCTTCCTA+TGG | + | Chr4:43258368-43258387 | MsG0480020552.01.T01:CDS | 45.0% |
| ! | TGGGTTGACAATTCTTGCAC+CGG | + | Chr4:43258065-43258084 | MsG0480020552.01.T01:CDS | 45.0% |
| !! | TCCTGCTTTGAGTTGTGAGA+AGG | - | Chr4:43258100-43258119 | None:intergenic | 45.0% |
| !!! | AAGTGGGCGTCTCTTGTTTT+TGG | + | Chr4:43258561-43258580 | MsG0480020552.01.T01:CDS | 45.0% |
| !!! | GCTTTTGGAGTCGTTGTTGA+AGG | - | Chr4:43258447-43258466 | None:intergenic | 45.0% |
| AACCCTGTGAGAACACTTGC+TGG | + | Chr4:43258216-43258235 | MsG0480020552.01.T01:CDS | 50.0% | |
| CTCCAGCAAGTGTTCTCACA+GGG | - | Chr4:43258221-43258240 | None:intergenic | 50.0% | |
| GCCATTATCGCCAGATTCCT+CGG | + | Chr4:43258479-43258498 | MsG0480020552.01.T01:CDS | 50.0% | |
| GCCTTCTCACAACTCAAAGC+AGG | + | Chr4:43258096-43258115 | MsG0480020552.01.T01:CDS | 50.0% | |
| GGGCTTAACAGATGAATCCG+AGG | - | Chr4:43258499-43258518 | None:intergenic | 50.0% | |
| GTCCATCGGAAAGTGAGTTG+AGG | - | Chr4:43258125-43258144 | None:intergenic | 50.0% | |
| TCCGAGGAATCTGGCGATAA+TGG | - | Chr4:43258483-43258502 | None:intergenic | 50.0% | |
| ! | ACACTTGCTGGAGCTAAACC+CGG | + | Chr4:43258228-43258247 | MsG0480020552.01.T01:CDS | 50.0% |
| !! | TGTGAGAAGGCGTTGTCATC+CGG | - | Chr4:43258087-43258106 | None:intergenic | 50.0% |
| ACCGACGAAGGGACTGTCTT+TGG | + | Chr4:43258413-43258432 | MsG0480020552.01.T01:CDS | 55.0% | |
| GCCAAAGACAGTCCCTTCGT+CGG | - | Chr4:43258417-43258436 | None:intergenic | 55.0% | |
| GCTCCAGCAAGTGTTCTCAC+AGG | - | Chr4:43258222-43258241 | None:intergenic | 55.0% | |
| GGAGTCGTTGTTGAAGGCTC+TGG | - | Chr4:43258441-43258460 | None:intergenic | 55.0% | |
| TGCTGGAGCTAAACCCGGAA+AGG | + | Chr4:43258233-43258252 | MsG0480020552.01.T01:CDS | 55.0% | |
| TGGAGCTAAACCCGGAAAGG+TGG | + | Chr4:43258236-43258255 | MsG0480020552.01.T01:CDS | 55.0% | |
| !! | GTTGCTAAGGCACCGACGAA+GGG | + | Chr4:43258402-43258421 | MsG0480020552.01.T01:CDS | 55.0% |
| !! | TGTTGCTAAGGCACCGACGA+AGG | + | Chr4:43258401-43258420 | MsG0480020552.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 43258006 | 43258614 | 43258006 | ID=MsG0480020552.01;Name=MsG0480020552.01 |
| Chr4 | mRNA | 43258006 | 43258614 | 43258006 | ID=MsG0480020552.01.T01;Parent=MsG0480020552.01;Name=MsG0480020552.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|202 |
| Chr4 | exon | 43258006 | 43258614 | 43258006 | ID=MsG0480020552.01.T01:exon:15631;Parent=MsG0480020552.01.T01 |
| Chr4 | CDS | 43258006 | 43258614 | 43258006 | ID=MsG0480020552.01.T01:cds;Parent=MsG0480020552.01.T01 |
| Gene Sequence |
| Protein sequence |
