Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020817.01.T01 | MCI05574.1 | 85.507 | 69 | 10 | 0 | 15 | 83 | 1 | 69 | 2.32E-31 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020817.01.T01 | A0A392P1I2 | 85.507 | 69 | 10 | 0 | 15 | 83 | 1 | 69 | 1.11e-31 | 114 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010891.01 | MsG0480020817.01 | 0.830681 | 2.560721e-55 | 2.717304e-52 |
| MsG0280010892.01 | MsG0480020817.01 | 0.861904 | 7.388250e-64 | 2.123338e-60 |
| MsG0280010893.01 | MsG0480020817.01 | 0.865328 | 6.397202e-65 | 2.073072e-61 |
| MsG0480020552.01 | MsG0480020817.01 | 0.918989 | 7.873870e-87 | 2.469379e-82 |
| MsG0480020815.01 | MsG0480020817.01 | 0.922994 | 4.757405e-89 | 1.857463e-84 |
| MsG0480020816.01 | MsG0480020817.01 | 0.881685 | 1.954418e-70 | 1.156097e-66 |
| MsG0480020817.01 | MsG0480020818.01 | 0.922146 | 1.436119e-88 | 5.341175e-84 |
| MsG0480020817.01 | MsG0480023097.01 | 0.800222 | 1.594437e-48 | 7.383611e-46 |
| MsG0480020817.01 | MsG0480023113.01 | 0.919985 | 2.267168e-87 | 7.495054e-83 |
| MsG0480020817.01 | MsG0480023114.01 | 0.936159 | 2.710376e-97 | 2.273958e-92 |
| MsG0480020817.01 | MsG0480023115.01 | 0.907516 | 4.656756e-81 | 8.286976e-77 |
| MsG0480020817.01 | MsG0480023116.01 | 0.919868 | 2.625356e-87 | 8.622170e-83 |
| MsG0480020817.01 | MsG0780040592.01 | 0.866871 | 2.076793e-65 | 7.108209e-62 |
| MsG0480020817.01 | MsG0880042557.01 | 0.831627 | 1.499495e-55 | 1.636417e-52 |
| MsG0480020817.01 | MsG0880044994.01 | 0.882861 | 7.301989e-71 | 4.522103e-67 |
| MsG0480020817.01 | MsG0880045339.01 | 0.938451 | 6.582294e-99 | 6.348170e-94 |
| MsG0480020817.01 | MsG0880047773.01 | 0.859019 | 5.525521e-63 | 1.438286e-59 |
| MsG0280006934.01 | MsG0480020817.01 | 0.832809 | 7.649187e-56 | 8.644322e-53 |
| MsG0280009327.01 | MsG0480020817.01 | 0.870054 | 1.949725e-66 | 7.480709e-63 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGCATTAGCTGGTGATAAT+TGG | 0.229276 | 4:-48822184 | MsG0480020817.01.T01:CDS |
| TCAACAGTGCCTGCGGTGTC+AGG | 0.354935 | 4:-48822299 | MsG0480020817.01.T01:CDS |
| AATGAAGGTACCAATGGTTT+AGG | 0.368336 | 4:-48822251 | MsG0480020817.01.T01:CDS |
| GCAGCTGGTGTGGTTGGAGC+TGG | 0.431548 | 4:-48822224 | MsG0480020817.01.T01:CDS |
| AGCATTAGCTGGTGATAATT+GGG | 0.432747 | 4:-48822183 | MsG0480020817.01.T01:CDS |
| GCTGGTAGTGTAGGTTGTAT+AGG | 0.438970 | 4:-48822206 | MsG0480020817.01.T01:CDS |
| TGTATGCCTTCGCCTTTCTC+AGG | 0.442653 | 4:-48822334 | MsG0480020817.01.T01:CDS |
| GGAGCTGCAGCTGGTGTGGT+TGG | 0.478200 | 4:-48822230 | MsG0480020817.01.T01:CDS |
| GTGGTTGGAGCTGGTAGTGT+AGG | 0.493589 | 4:-48822215 | MsG0480020817.01.T01:CDS |
| CAACAGTGCCTGCGGTGTCA+GGG | 0.508035 | 4:-48822298 | MsG0480020817.01.T01:CDS |
| TTGGGATAAAGTAGTGGTGG+TGG | 0.526558 | 4:-48822165 | MsG0480020817.01.T01:CDS |
| CTAGGTGACTCTGGTAATGA+AGG | 0.531327 | 4:-48822266 | MsG0480020817.01.T01:CDS |
| AGTGCCTGCGGTGTCAGGGG+TGG | 0.531927 | 4:-48822294 | MsG0480020817.01.T01:CDS |
| GCAGGCACTGTTGACATTGT+CGG | 0.533562 | 4:+48822308 | None:intergenic |
| AGCTGCAGCTCCTAAACCAT+TGG | 0.544279 | 4:+48822241 | None:intergenic |
| GTGGAATCACTAGGTGACTC+TGG | 0.548332 | 4:-48822275 | MsG0480020817.01.T01:CDS |
| CATTGTCGGAATCCTGAGAA+AGG | 0.566305 | 4:+48822322 | None:intergenic |
| AACTAGTAGTGCAAGTGAGA+AGG | 0.570303 | 4:-48822129 | MsG0480020817.01.T01:CDS |
| GGTTGTATAGGAGCATTAGC+TGG | 0.571828 | 4:-48822194 | MsG0480020817.01.T01:CDS |
| GATTCCACCCCTGACACCGC+AGG | 0.578533 | 4:+48822290 | None:intergenic |
| GTGTCAGGGGTGGAATCACT+AGG | 0.582776 | 4:-48822284 | MsG0480020817.01.T01:CDS |
| TCTGGTAATGAAGGTACCAA+TGG | 0.585762 | 4:-48822257 | MsG0480020817.01.T01:CDS |
| AACAGTGCCTGCGGTGTCAG+GGG | 0.587591 | 4:-48822297 | MsG0480020817.01.T01:CDS |
| AATGGTTTAGGAGCTGCAGC+TGG | 0.597225 | 4:-48822239 | MsG0480020817.01.T01:CDS |
| TTTAGGAGCTGCAGCTGGTG+TGG | 0.597546 | 4:-48822234 | MsG0480020817.01.T01:CDS |
| CGGAATCCTGAGAAAGGCGA+AGG | 0.598403 | 4:+48822328 | None:intergenic |
| GACAATGTCAACAGTGCCTG+CGG | 0.617535 | 4:-48822306 | MsG0480020817.01.T01:CDS |
| TAATTGGGATAAAGTAGTGG+TGG | 0.657413 | 4:-48822168 | MsG0480020817.01.T01:CDS |
| TGATAATTGGGATAAAGTAG+TGG | 0.662459 | 4:-48822171 | MsG0480020817.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TGATAATTGGGATAAAGTAG+TGG | - | Chr4:48822288-48822307 | MsG0480020817.01.T01:CDS | 30.0% | |
| AGCATTAGCTGGTGATAATT+GGG | - | Chr4:48822276-48822295 | MsG0480020817.01.T01:CDS | 35.0% | |
| TAATTGGGATAAAGTAGTGG+TGG | - | Chr4:48822291-48822310 | MsG0480020817.01.T01:CDS | 35.0% | |
| !! | AATGAAGGTACCAATGGTTT+AGG | - | Chr4:48822208-48822227 | MsG0480020817.01.T01:CDS | 35.0% |
| AACTAGTAGTGCAAGTGAGA+AGG | - | Chr4:48822330-48822349 | MsG0480020817.01.T01:CDS | 40.0% | |
| GAGCATTAGCTGGTGATAAT+TGG | - | Chr4:48822275-48822294 | MsG0480020817.01.T01:CDS | 40.0% | |
| !! | TCTGGTAATGAAGGTACCAA+TGG | - | Chr4:48822202-48822221 | MsG0480020817.01.T01:CDS | 40.0% |
| CATTGTCGGAATCCTGAGAA+AGG | + | Chr4:48822140-48822159 | None:intergenic | 45.0% | |
| TTGGGATAAAGTAGTGGTGG+TGG | - | Chr4:48822294-48822313 | MsG0480020817.01.T01:CDS | 45.0% | |
| ! | CTAGGTGACTCTGGTAATGA+AGG | - | Chr4:48822193-48822212 | MsG0480020817.01.T01:CDS | 45.0% |
| ! | GCTGGTAGTGTAGGTTGTAT+AGG | - | Chr4:48822253-48822272 | MsG0480020817.01.T01:CDS | 45.0% |
| !! | GGTTGTATAGGAGCATTAGC+TGG | - | Chr4:48822265-48822284 | MsG0480020817.01.T01:CDS | 45.0% |
| AATGGTTTAGGAGCTGCAGC+TGG | - | Chr4:48822220-48822239 | MsG0480020817.01.T01:CDS | 50.0% | |
| AGCTGCAGCTCCTAAACCAT+TGG | + | Chr4:48822221-48822240 | None:intergenic | 50.0% | |
| GACAATGTCAACAGTGCCTG+CGG | - | Chr4:48822153-48822172 | MsG0480020817.01.T01:CDS | 50.0% | |
| GTGGAATCACTAGGTGACTC+TGG | - | Chr4:48822184-48822203 | MsG0480020817.01.T01:CDS | 50.0% | |
| TGTATGCCTTCGCCTTTCTC+AGG | - | Chr4:48822125-48822144 | MsG0480020817.01.T01:CDS | 50.0% | |
| ! | GCAGGCACTGTTGACATTGT+CGG | + | Chr4:48822154-48822173 | None:intergenic | 50.0% |
| CGGAATCCTGAGAAAGGCGA+AGG | + | Chr4:48822134-48822153 | None:intergenic | 55.0% | |
| GTGTCAGGGGTGGAATCACT+AGG | - | Chr4:48822175-48822194 | MsG0480020817.01.T01:CDS | 55.0% | |
| TTTAGGAGCTGCAGCTGGTG+TGG | - | Chr4:48822225-48822244 | MsG0480020817.01.T01:CDS | 55.0% | |
| ! | GTGGTTGGAGCTGGTAGTGT+AGG | - | Chr4:48822244-48822263 | MsG0480020817.01.T01:CDS | 55.0% |
| TCAACAGTGCCTGCGGTGTC+AGG | - | Chr4:48822160-48822179 | MsG0480020817.01.T01:CDS | 60.0% | |
| ! | AACAGTGCCTGCGGTGTCAG+GGG | - | Chr4:48822162-48822181 | MsG0480020817.01.T01:CDS | 60.0% |
| ! | CAACAGTGCCTGCGGTGTCA+GGG | - | Chr4:48822161-48822180 | MsG0480020817.01.T01:CDS | 60.0% |
| GATTCCACCCCTGACACCGC+AGG | + | Chr4:48822172-48822191 | None:intergenic | 65.0% | |
| GCAGCTGGTGTGGTTGGAGC+TGG | - | Chr4:48822235-48822254 | MsG0480020817.01.T01:CDS | 65.0% | |
| GGAGCTGCAGCTGGTGTGGT+TGG | - | Chr4:48822229-48822248 | MsG0480020817.01.T01:CDS | 65.0% | |
| ! | AGTGCCTGCGGTGTCAGGGG+TGG | - | Chr4:48822165-48822184 | MsG0480020817.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 48822115 | 48822366 | 48822115 | ID=MsG0480020817.01;Name=MsG0480020817.01 |
| Chr4 | mRNA | 48822115 | 48822366 | 48822115 | ID=MsG0480020817.01.T01;Parent=MsG0480020817.01;Name=MsG0480020817.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|83 |
| Chr4 | exon | 48822115 | 48822366 | 48822115 | ID=MsG0480020817.01.T01:exon:2595;Parent=MsG0480020817.01.T01 |
| Chr4 | CDS | 48822115 | 48822366 | 48822115 | ID=MsG0480020817.01.T01:cds;Parent=MsG0480020817.01.T01 |
| Gene Sequence |
| Protein sequence |
