Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023115.01.T01 | XP_003629840.2 | 94.444 | 108 | 5 | 1 | 1 | 107 | 209 | 316 | 3.32E-48 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023115.01.T01 | B7FJN1 | 94.444 | 108 | 5 | 1 | 1 | 107 | 209 | 316 | 1.59e-48 | 166 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010891.01 | MsG0480023115.01 | 0.846256 | 2.434271e-59 | 4.165086e-56 |
| MsG0280010892.01 | MsG0480023115.01 | 0.845106 | 4.994197e-59 | 8.234183e-56 |
| MsG0480020552.01 | MsG0480023115.01 | 0.916247 | 2.240616e-85 | 6.107834e-81 |
| MsG0480020815.01 | MsG0480023115.01 | 0.868209 | 7.741933e-66 | 2.777682e-62 |
| MsG0480020816.01 | MsG0480023115.01 | 0.903471 | 3.352023e-79 | 4.969573e-75 |
| MsG0480020817.01 | MsG0480023115.01 | 0.907516 | 4.656756e-81 | 8.286976e-77 |
| MsG0480020818.01 | MsG0480023115.01 | 0.923468 | 2.553448e-89 | 1.023710e-84 |
| MsG0480023113.01 | MsG0480023115.01 | 0.870291 | 1.630371e-66 | 6.309070e-63 |
| MsG0480023114.01 | MsG0480023115.01 | 0.900335 | 8.118731e-78 | 1.045329e-73 |
| MsG0480023115.01 | MsG0480023116.01 | 0.918691 | 1.140040e-86 | 3.517430e-82 |
| MsG0480023115.01 | MsG0780040592.01 | 0.820942 | 5.254059e-53 | 4.216119e-50 |
| MsG0480023115.01 | MsG0880042557.01 | 0.849810 | 2.546157e-60 | 4.885694e-57 |
| MsG0480023115.01 | MsG0880044994.01 | 0.849589 | 2.935165e-60 | 5.591110e-57 |
| MsG0480023115.01 | MsG0880045339.01 | 0.929666 | 5.007569e-93 | 2.831947e-88 |
| MsG0480023115.01 | MsG0880047773.01 | 0.893228 | 7.641623e-75 | 7.219970e-71 |
| MsG0280006934.01 | MsG0480023115.01 | 0.824606 | 7.370692e-54 | 6.561205e-51 |
| MsG0280009327.01 | MsG0480023115.01 | 0.832015 | 1.202828e-55 | 1.327814e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023115.01.T01 | MTR_8g087460 | 94.444 | 108 | 5 | 1 | 1 | 107 | 209 | 316 | 4.02e-52 | 166 |
| MsG0480023115.01.T01 | MTR_8g087470 | 95.370 | 108 | 4 | 1 | 1 | 107 | 209 | 316 | 2.34e-51 | 164 |
| MsG0480023115.01.T01 | MTR_2g017950 | 54.955 | 111 | 33 | 4 | 1 | 104 | 191 | 291 | 2.79e-20 | 83.2 |
| MsG0480023115.01.T01 | MTR_2g017960 | 40.367 | 109 | 46 | 3 | 1 | 103 | 182 | 277 | 1.35e-14 | 67.8 |
| MsG0480023115.01.T01 | MTR_2g017970 | 42.453 | 106 | 47 | 3 | 1 | 106 | 182 | 273 | 1.60e-14 | 67.4 |
| MsG0480023115.01.T01 | MTR_4g059840 | 50.746 | 67 | 27 | 2 | 1 | 61 | 187 | 253 | 4.39e-12 | 60.8 |
| MsG0480023115.01.T01 | MTR_4g059790 | 49.254 | 67 | 28 | 2 | 1 | 61 | 187 | 253 | 6.78e-12 | 60.5 |
| MsG0480023115.01.T01 | MTR_4g053380 | 52.459 | 61 | 24 | 2 | 1 | 57 | 191 | 250 | 1.48e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCTCTTGATCTTGTTCTTC+CGG | 0.272392 | 4:+81090903 | MsG0480023115.01.T01:CDS |
| TAGCAAACTTGCTATTTATC+AGG | 0.275231 | 4:+81090864 | MsG0480023115.01.T01:CDS |
| CCTGCTGATGTTTCTGCTGC+AGG | 0.329762 | 4:+81091051 | MsG0480023115.01.T01:CDS |
| GTTGGTGGAATTGTTCTATA+AGG | 0.340654 | 4:+81091117 | MsG0480023115.01.T01:CDS |
| GCAGTTAATGCTACCGTTAC+CGG | 0.364782 | 4:+81090826 | MsG0480023115.01.T01:CDS |
| TCTGGCTCCGGCGCCAGCTA+AGG | 0.394000 | 4:+81090939 | MsG0480023115.01.T01:CDS |
| TGCTATTTATCAGGTTGATA+AGG | 0.394299 | 4:+81090873 | MsG0480023115.01.T01:CDS |
| TGCTGCAGGGTCTGTGATGT+GGG | 0.418106 | 4:+81091065 | MsG0480023115.01.T01:CDS |
| TGATCTTGTTCTTCCGGCTA+AGG | 0.420269 | 4:+81090909 | MsG0480023115.01.T01:CDS |
| TCCGGCTAAGGCTCCATCTC+TGG | 0.421914 | 4:+81090921 | MsG0480023115.01.T01:CDS |
| AAGGGTTTGTTGCCTAAGGC+TGG | 0.443081 | 4:+81090958 | MsG0480023115.01.T01:CDS |
| CTGGCTCCGGCGCCAGCTAA+GGG | 0.452323 | 4:+81090940 | MsG0480023115.01.T01:CDS |
| CTGCTGCAGGGTCTGTGATG+TGG | 0.454235 | 4:+81091064 | MsG0480023115.01.T01:CDS |
| AGCTAAGGGTTTGTTGCCTA+AGG | 0.460321 | 4:+81090954 | MsG0480023115.01.T01:CDS |
| CTGCTGATGTTTCTGCTGCA+GGG | 0.487153 | 4:+81091052 | MsG0480023115.01.T01:CDS |
| TTAGGCAACAAACCCTTAGC+TGG | 0.492766 | 4:-81090952 | None:intergenic |
| AACAAACCCTTAGCTGGCGC+CGG | 0.504015 | 4:-81090946 | None:intergenic |
| GTAGACGATGGCAGTGGTGC+TGG | 0.509847 | 4:+81091000 | MsG0480023115.01.T01:CDS |
| GCCAGAGATGGAGCCTTAGC+CGG | 0.520251 | 4:-81090922 | None:intergenic |
| GCTGGCGCCGGAGCCAGAGA+TGG | 0.521879 | 4:-81090934 | None:intergenic |
| TAAGGCTCCATCTCTGGCTC+CGG | 0.530879 | 4:+81090927 | MsG0480023115.01.T01:CDS |
| AACGGTAGCATTAACTGCTC+CGG | 0.540707 | 4:-81090821 | None:intergenic |
| TTTGGAATGAGTTTGGTTGG+TGG | 0.550464 | 4:+81091102 | MsG0480023115.01.T01:CDS |
| AGTGGTGCTGGTAGTGATGA+TGG | 0.554877 | 4:+81091012 | MsG0480023115.01.T01:CDS |
| ATAAATAGCAAGTTTGCTAT+CGG | 0.562446 | 4:-81090860 | None:intergenic |
| GGTATAAACAGTACCGGTAA+CGG | 0.565166 | 4:-81090839 | None:intergenic |
| TCGGTTGTAGACGATGGCAG+TGG | 0.591876 | 4:+81090994 | MsG0480023115.01.T01:CDS |
| AGTCAAGTTAGTATGGCTAC+CGG | 0.601147 | 4:+81090802 | None:intergenic |
| GCTATCGGTATAAACAGTAC+CGG | 0.606873 | 4:-81090845 | None:intergenic |
| GCCGGAAGAACAAGATCAAG+AGG | 0.613702 | 4:-81090904 | None:intergenic |
| CCTGCAGCAGAAACATCAGC+AGG | 0.614736 | 4:-81091051 | None:intergenic |
| TAGTGATGATGGTGATGGCA+AGG | 0.668905 | 4:+81091023 | MsG0480023115.01.T01:CDS |
| AATTCATCGGTTGTAGACGA+TGG | 0.674617 | 4:+81090988 | MsG0480023115.01.T01:CDS |
| GCTGGTAGTGATGATGGTGA+TGG | 0.674633 | 4:+81091018 | MsG0480023115.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAATAGCAAGTTTGCTAT+CGG | - | Chr4:81090863-81090882 | None:intergenic | 25.0% |
| !! | TAGCAAACTTGCTATTTATC+AGG | + | Chr4:81090864-81090883 | MsG0480023115.01.T01:CDS | 30.0% |
| !! | TGCTATTTATCAGGTTGATA+AGG | + | Chr4:81090873-81090892 | MsG0480023115.01.T01:CDS | 30.0% |
| !!! | TGTTGTTTTTGGAATGAGTT+TGG | + | Chr4:81091095-81091114 | MsG0480023115.01.T01:CDS | 30.0% |
| GGCTGGAAAAACTAATTCAT+CGG | + | Chr4:81090975-81090994 | MsG0480023115.01.T01:CDS | 35.0% | |
| !!! | GAATTAGTTTTTCCAGCCTT+AGG | - | Chr4:81090973-81090992 | None:intergenic | 35.0% |
| !!! | GTTTTTGGAATGAGTTTGGT+TGG | + | Chr4:81091099-81091118 | MsG0480023115.01.T01:CDS | 35.0% |
| !!! | TGGGTGAATGTTGTTGTTTT+TGG | + | Chr4:81091084-81091103 | MsG0480023115.01.T01:CDS | 35.0% |
| GCTATCGGTATAAACAGTAC+CGG | - | Chr4:81090848-81090867 | None:intergenic | 40.0% | |
| ! | AATTCATCGGTTGTAGACGA+TGG | + | Chr4:81090988-81091007 | MsG0480023115.01.T01:CDS | 40.0% |
| ! | TTTGGAATGAGTTTGGTTGG+TGG | + | Chr4:81091102-81091121 | MsG0480023115.01.T01:CDS | 40.0% |
| !! | GGTATAAACAGTACCGGTAA+CGG | - | Chr4:81090842-81090861 | None:intergenic | 40.0% |
| AACGGTAGCATTAACTGCTC+CGG | - | Chr4:81090824-81090843 | None:intergenic | 45.0% | |
| GCAGTTAATGCTACCGTTAC+CGG | + | Chr4:81090826-81090845 | MsG0480023115.01.T01:CDS | 45.0% | |
| GCCTCTTGATCTTGTTCTTC+CGG | + | Chr4:81090903-81090922 | MsG0480023115.01.T01:CDS | 45.0% | |
| TGATCTTGTTCTTCCGGCTA+AGG | + | Chr4:81090909-81090928 | MsG0480023115.01.T01:CDS | 45.0% | |
| TTAGGCAACAAACCCTTAGC+TGG | - | Chr4:81090955-81090974 | None:intergenic | 45.0% | |
| !! | AGCTAAGGGTTTGTTGCCTA+AGG | + | Chr4:81090954-81090973 | MsG0480023115.01.T01:CDS | 45.0% |
| !! | TAGTGATGATGGTGATGGCA+AGG | + | Chr4:81091023-81091042 | MsG0480023115.01.T01:CDS | 45.0% |
| GCCGGAAGAACAAGATCAAG+AGG | - | Chr4:81090907-81090926 | None:intergenic | 50.0% | |
| ! | CTGCTGATGTTTCTGCTGCA+GGG | + | Chr4:81091052-81091071 | MsG0480023115.01.T01:CDS | 50.0% |
| !! | AAGGGTTTGTTGCCTAAGGC+TGG | + | Chr4:81090958-81090977 | MsG0480023115.01.T01:CDS | 50.0% |
| !! | AGTGGTGCTGGTAGTGATGA+TGG | + | Chr4:81091012-81091031 | MsG0480023115.01.T01:CDS | 50.0% |
| !! | GCTGGTAGTGATGATGGTGA+TGG | + | Chr4:81091018-81091037 | MsG0480023115.01.T01:CDS | 50.0% |
| AACAAACCCTTAGCTGGCGC+CGG | - | Chr4:81090949-81090968 | None:intergenic | 55.0% | |
| CCTGCAGCAGAAACATCAGC+AGG | - | Chr4:81091054-81091073 | None:intergenic | 55.0% | |
| TCGGTTGTAGACGATGGCAG+TGG | + | Chr4:81090994-81091013 | MsG0480023115.01.T01:CDS | 55.0% | |
| TGCTGCAGGGTCTGTGATGT+GGG | + | Chr4:81091065-81091084 | MsG0480023115.01.T01:CDS | 55.0% | |
| ! | CCTGCTGATGTTTCTGCTGC+AGG | + | Chr4:81091051-81091070 | MsG0480023115.01.T01:CDS | 55.0% |
| ! | TAAGGCTCCATCTCTGGCTC+CGG | + | Chr4:81090927-81090946 | MsG0480023115.01.T01:CDS | 55.0% |
| CTGCTGCAGGGTCTGTGATG+TGG | + | Chr4:81091064-81091083 | MsG0480023115.01.T01:CDS | 60.0% | |
| GCCAGAGATGGAGCCTTAGC+CGG | - | Chr4:81090925-81090944 | None:intergenic | 60.0% | |
| GTAGACGATGGCAGTGGTGC+TGG | + | Chr4:81091000-81091019 | MsG0480023115.01.T01:CDS | 60.0% | |
| TCCGGCTAAGGCTCCATCTC+TGG | + | Chr4:81090921-81090940 | MsG0480023115.01.T01:CDS | 60.0% | |
| CTGGCTCCGGCGCCAGCTAA+GGG | + | Chr4:81090940-81090959 | MsG0480023115.01.T01:CDS | 70.0% | |
| ! | TCTGGCTCCGGCGCCAGCTA+AGG | + | Chr4:81090939-81090958 | MsG0480023115.01.T01:CDS | 70.0% |
| ! | GCTGGCGCCGGAGCCAGAGA+TGG | - | Chr4:81090937-81090956 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 81090814 | 81091137 | 81090814 | ID=MsG0480023115.01;Name=MsG0480023115.01 |
| Chr4 | mRNA | 81090814 | 81091137 | 81090814 | ID=MsG0480023115.01.T01;Parent=MsG0480023115.01;Name=MsG0480023115.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|107 |
| Chr4 | exon | 81090814 | 81091137 | 81090814 | ID=MsG0480023115.01.T01:exon:2297;Parent=MsG0480023115.01.T01 |
| Chr4 | CDS | 81090814 | 81091137 | 81090814 | ID=MsG0480023115.01.T01:cds;Parent=MsG0480023115.01.T01 |
| Gene Sequence |
| Protein sequence |
