AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023113.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023113.01.T01 MTR_8g087450 96.918 292 9 0 1 292 1 292 0.0 566
MsG0480023113.01.T01 MTR_2g017960 71.379 290 66 6 1 289 1 274 3.21e-116 335
MsG0480023113.01.T01 MTR_2g017970 77.143 210 46 2 76 285 67 274 1.67e-109 318
MsG0480023113.01.T01 MTR_4g059840 55.187 241 93 6 58 288 53 288 3.64e-80 244
MsG0480023113.01.T01 MTR_4g053380 49.836 305 121 8 2 291 7 294 2.29e-78 240
MsG0480023113.01.T01 MTR_4g059790 60.199 201 70 3 58 248 53 253 5.69e-78 239
MsG0480023113.01.T01 MTR_2g093980 48.814 295 130 9 1 288 1 281 1.01e-75 233
MsG0480023113.01.T01 MTR_4g059780 49.815 271 115 5 21 284 45 301 9.30e-69 216
MsG0480023113.01.T01 MTR_2g093970 45.890 292 125 7 10 285 13 287 7.46e-68 213
MsG0480023113.01.T01 MTR_2g017950 43.345 293 139 8 6 285 12 290 2.75e-64 204
MsG0480023113.01.T01 MTR_8g087470 45.113 266 127 6 41 288 51 315 1.08e-62 201
MsG0480023113.01.T01 MTR_8g087460 46.212 264 121 7 45 288 53 315 1.09e-61 198
MsG0480023113.01.T01 MTR_4g059720 45.415 229 84 4 1 228 4 192 1.07e-56 183
MsG0480023113.01.T01 MTR_2g093990 46.117 206 101 3 77 279 41 239 7.45e-53 173
MsG0480023113.01.T01 MTR_7g101080 44.578 166 90 2 58 222 21 185 2.42e-41 144
MsG0480023113.01.T01 MTR_2g090765 35.565 239 132 4 50 286 29 247 1.45e-27 107
MsG0480023113.01.T01 MTR_5g098060 30.588 170 113 3 77 243 48 215 1.08e-15 75.5
MsG0480023113.01.T01 MTR_7g075453 31.746 189 113 4 68 250 175 353 3.73e-14 72.4
MsG0480023113.01.T01 MTR_2g437700 31.544 149 95 4 77 221 195 340 1.90e-12 67.4
MsG0480023113.01.T01 MTR_5g098420 30.270 185 114 5 55 235 177 350 7.29e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023113.01.T01 AT5G60490 54.795 146 64 2 77 221 39 183 7.51e-47 158
MsG0480023113.01.T01 AT5G03170 46.023 176 93 2 70 244 29 203 7.41e-45 152
MsG0480023113.01.T01 AT5G44130 40.437 183 103 3 77 257 36 214 1.13e-33 123
MsG0480023113.01.T01 AT2G20520 41.622 185 95 5 77 249 38 221 1.37e-29 112
MsG0480023113.01.T01 AT1G03870 38.830 188 109 4 77 263 39 221 2.91e-29 112
MsG0480023113.01.T01 AT2G04780 31.544 149 96 3 75 221 44 188 2.68e-13 68.9
MsG0480023113.01.T01 AT2G04780 31.544 149 96 3 75 221 44 188 2.68e-13 68.9
MsG0480023113.01.T01 AT3G46550 28.495 186 120 8 63 243 194 371 1.07e-12 68.2
MsG0480023113.01.T01 AT3G60900 28.488 172 117 3 70 240 183 349 3.31e-12 67.0

Find 49 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGCTGGAGCTTTAGCTTT+AGG 0.270473 4:-81088087 None:intergenic
TTCGAACTTGATACATATTC+AGG 0.279696 4:-81087862 None:intergenic
GAATTCTCTTTCTCAGCTTT+AGG 0.296501 4:-81088126 None:intergenic
TGCTATATATCATGTGAATA+AGG 0.305049 4:+81088038 MsG0480023113.01.T01:CDS
CAGATTAATGTGACTGCATA+TGG 0.308276 4:+81087943 MsG0480023113.01.T01:CDS
GGTGCTGGAGCAGGTGTTGA+AGG 0.331645 4:-81087547 MsG0480023112.01.T01:CDS
ATGCATGTTATTCAAGCTAA+TGG 0.337878 4:-81088199 None:intergenic
GAATAAGGTTCTTATTCCTT+TGG 0.338628 4:+81088053 MsG0480023113.01.T01:CDS
TCTTGGTTTCCTGACGAATC+AGG 0.343060 4:-81087616 MsG0480023112.01.T01:CDS
AGCAGGTTACTTCAACTCCT+TGG 0.397163 4:+81087798 MsG0480023113.01.T01:CDS
TGTGGTAATGCTGGGACTAA+TGG 0.407871 4:-81087577 MsG0480023112.01.T01:CDS
GATTAGTGTGTTGAATGATT+TGG 0.407894 4:-81087662 MsG0480023112.01.T01:CDS
TTAGAATCTTGATTATGTCT+TGG 0.417666 4:-81087633 MsG0480023112.01.T01:CDS
GCCTTAGCAAGAGCTGGTGC+TGG 0.431209 4:-81088102 None:intergenic
TCTGAAAAGGCACCATCATC+TGG 0.442423 4:-81087769 None:intergenic
GTGCTGGAGCAGGTGTTGAA+GGG 0.442970 4:-81087546 MsG0480023112.01.T01:CDS
AGAAGATAGAGGCAAGTAGT+AGG 0.446057 4:-81087435 None:intergenic
ATATCAGTGTTGGTTTCCCT+TGG 0.450067 4:+81088225 MsG0480023113.01.T01:CDS
GGAGCAGGTGTTGAAGGGGT+TGG 0.451921 4:-81087541 MsG0480023112.01.T01:CDS
GCAGGTTACTTCAACTCCTT+GGG 0.455965 4:+81087799 MsG0480023113.01.T01:CDS
AACAGTGTGAATATTTCAAC+TGG 0.459076 4:+81087967 MsG0480023113.01.T01:CDS
GTAGGTGATTGTGGTAATGC+TGG 0.464029 4:-81087586 MsG0480023112.01.T01:CDS
GACGAATCAGGATTTGTTGT+AGG 0.471829 4:-81087604 MsG0480023112.01.T01:CDS
CATTAATCTGAAATCCTTGT+GGG 0.483229 4:-81087930 None:intergenic
ACCTGCTTTGAGTTCTGAAA+AGG 0.484192 4:-81087782 None:intergenic
TTAGGTGCCTTAGCAAGAGC+TGG 0.489973 4:-81088108 None:intergenic
GGATTTGTTGTAGGTGATTG+TGG 0.513333 4:-81087595 MsG0480023112.01.T01:CDS
ACAATTCTTGCACCAGATGA+TGG 0.514841 4:+81087757 MsG0480023113.01.T01:CDS
ACAACAAATCCTGATTCGTC+AGG 0.522883 4:+81087607 MsG0480023113.01.T01:CDS
AGTGGTGATTGAGAAGATAG+AGG 0.531658 4:-81087446 None:intergenic
TTTGTGTTGTCTTGAGCAAA+CGG 0.534377 4:-81087684 MsG0480023112.01.T01:CDS
TCCAGCACCAGCTCTTGCTA+AGG 0.542557 4:+81088101 MsG0480023113.01.T01:CDS
AGCGTAAACGATTCCAGTGA+TGG 0.545525 4:-81088004 None:intergenic
TTACAAGCTGTGCATTGACT+TGG 0.551173 4:-81087714 None:intergenic
TGCTGGAGCAGGTGTTGAAG+GGG 0.559876 4:-81087545 MsG0480023112.01.T01:CDS
CATGCATGGAATATCAGTGT+TGG 0.573529 4:+81088215 MsG0480023113.01.T01:CDS
ACATTAATCTGAAATCCTTG+TGG 0.574915 4:-81087931 None:intergenic
CTGCAAGCAGAGAAATTCCA+AGG 0.575775 4:-81088242 None:intergenic
AGCTGGTGATTGAGCTAAAG+TGG 0.578327 4:-81087464 None:intergenic
ATTAGCTTGAATAACATGCA+TGG 0.579278 4:+81088201 MsG0480023113.01.T01:CDS
TAGGTGATTGTGGTAATGCT+GGG 0.582717 4:-81087585 MsG0480023112.01.T01:CDS
GTTGTTGATACAACCATCAC+TGG 0.593007 4:+81087991 MsG0480023113.01.T01:CDS
TTAGGCTTAACAAAATCCAA+AGG 0.601169 4:-81088069 None:intergenic
CTAGCTAGTGATAGCCCACA+AGG 0.607212 4:+81087916 MsG0480023113.01.T01:CDS
TGATCAGACTCAGGCACACA+AGG 0.622744 4:+81088164 MsG0480023113.01.T01:CDS
AGATGATGATGATCAGACTC+AGG 0.626792 4:+81088155 MsG0480023113.01.T01:CDS
CACTAGCTAGTGTCAACACA+GGG 0.648522 4:-81087903 None:intergenic
TGCAAGCAGAGAAATTCCAA+GGG 0.658685 4:-81088241 None:intergenic
TCACTAGCTAGTGTCAACAC+AGG 0.685638 4:-81087904 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGCTATATATCATGTGAATA+AGG + Chr4:81088038-81088057 MsG0480023113.01.T01:CDS 25.0%
! TTAGAATCTTGATTATGTCT+TGG - Chr4:81087636-81087655 MsG0480023112.01.T01:CDS 25.0%
AACAGTGTGAATATTTCAAC+TGG + Chr4:81087967-81087986 MsG0480023113.01.T01:CDS 30.0%
ATGCATGTTATTCAAGCTAA+TGG - Chr4:81088202-81088221 None:intergenic 30.0%
CAGCTTGTAACTACAAAAAA+TGG + Chr4:81087727-81087746 MsG0480023113.01.T01:CDS 30.0%
CTTGTAACTACAAAAAATGG+AGG + Chr4:81087730-81087749 MsG0480023113.01.T01:CDS 30.0%
GAATAAGGTTCTTATTCCTT+TGG + Chr4:81088053-81088072 MsG0480023113.01.T01:CDS 30.0%
GATTAGTGTGTTGAATGATT+TGG - Chr4:81087665-81087684 MsG0480023112.01.T01:CDS 30.0%
TTAGGCTTAACAAAATCCAA+AGG - Chr4:81088072-81088091 None:intergenic 30.0%
TTCGAACTTGATACATATTC+AGG - Chr4:81087865-81087884 None:intergenic 30.0%
! ACATTAATCTGAAATCCTTG+TGG - Chr4:81087934-81087953 None:intergenic 30.0%
! ATTAGCTTGAATAACATGCA+TGG + Chr4:81088201-81088220 MsG0480023113.01.T01:CDS 30.0%
! CATTAATCTGAAATCCTTGT+GGG - Chr4:81087933-81087952 None:intergenic 30.0%
CAGATTAATGTGACTGCATA+TGG + Chr4:81087943-81087962 MsG0480023113.01.T01:CDS 35.0%
GAATTCTCTTTCTCAGCTTT+AGG - Chr4:81088129-81088148 None:intergenic 35.0%
TTTGTGTTGTCTTGAGCAAA+CGG - Chr4:81087687-81087706 MsG0480023112.01.T01:CDS 35.0%
!! TTTTTGTTGTCTTTCTCCCA+AGG - Chr4:81087818-81087837 None:intergenic 35.0%
ACAATTCTTGCACCAGATGA+TGG + Chr4:81087757-81087776 MsG0480023113.01.T01:CDS 40.0%
AGAAGATAGAGGCAAGTAGT+AGG - Chr4:81087438-81087457 None:intergenic 40.0%
AGTGGTGATTGAGAAGATAG+AGG - Chr4:81087449-81087468 None:intergenic 40.0%
CATGCATGGAATATCAGTGT+TGG + Chr4:81088215-81088234 MsG0480023113.01.T01:CDS 40.0%
GTTGTTGATACAACCATCAC+TGG + Chr4:81087991-81088010 MsG0480023113.01.T01:CDS 40.0%
TGCAAGCAGAGAAATTCCAA+GGG - Chr4:81088244-81088263 None:intergenic 40.0%
! ACAACAAATCCTGATTCGTC+AGG + Chr4:81087607-81087626 MsG0480023113.01.T01:CDS 40.0%
! AGATGATGATGATCAGACTC+AGG + Chr4:81088155-81088174 MsG0480023113.01.T01:CDS 40.0%
! TAGGTGATTGTGGTAATGCT+GGG - Chr4:81087588-81087607 MsG0480023112.01.T01:CDS 40.0%
!! ACCTGCTTTGAGTTCTGAAA+AGG - Chr4:81087785-81087804 None:intergenic 40.0%
!! ATATCAGTGTTGGTTTCCCT+TGG + Chr4:81088225-81088244 MsG0480023113.01.T01:CDS 40.0%
!! GACGAATCAGGATTTGTTGT+AGG - Chr4:81087607-81087626 MsG0480023112.01.T01:CDS 40.0%
!! GGATTTGTTGTAGGTGATTG+TGG - Chr4:81087598-81087617 MsG0480023112.01.T01:CDS 40.0%
!! TTACAAGCTGTGCATTGACT+TGG - Chr4:81087717-81087736 None:intergenic 40.0%
!!! TTGAAGGGGTTGGTTTTGTA+GGG - Chr4:81087534-81087553 MsG0480023112.01.T01:CDS 40.0%
!!! TTTGTAGGTGCTGGTTTTTC+TGG - Chr4:81087505-81087524 MsG0480023112.01.T01:CDS 40.0%
AGCAGGTTACTTCAACTCCT+TGG + Chr4:81087798-81087817 MsG0480023113.01.T01:CDS 45.0%
AGCGTAAACGATTCCAGTGA+TGG - Chr4:81088007-81088026 None:intergenic 45.0%
AGCTGGTGATTGAGCTAAAG+TGG - Chr4:81087467-81087486 None:intergenic 45.0%
CACTAGCTAGTGTCAACACA+GGG - Chr4:81087906-81087925 None:intergenic 45.0%
CTGCAAGCAGAGAAATTCCA+AGG - Chr4:81088245-81088264 None:intergenic 45.0%
GCAGGTTACTTCAACTCCTT+GGG + Chr4:81087799-81087818 MsG0480023113.01.T01:CDS 45.0%
TCACTAGCTAGTGTCAACAC+AGG - Chr4:81087907-81087926 None:intergenic 45.0%
TCTTGGTTTCCTGACGAATC+AGG - Chr4:81087619-81087638 MsG0480023112.01.T01:CDS 45.0%
! GCCTTTTCAGAACTCAAAGC+AGG + Chr4:81087781-81087800 MsG0480023113.01.T01:CDS 45.0%
! GTAGGTGATTGTGGTAATGC+TGG - Chr4:81087589-81087608 MsG0480023112.01.T01:CDS 45.0%
! TGTGGTAATGCTGGGACTAA+TGG - Chr4:81087580-81087599 MsG0480023112.01.T01:CDS 45.0%
!! ACTAATGGTTTTGCTGGTGC+TGG - Chr4:81087565-81087584 MsG0480023112.01.T01:CDS 45.0%
!! TCTGAAAAGGCACCATCATC+TGG - Chr4:81087772-81087791 None:intergenic 45.0%
!!! GCTGGTTTTTCTGGTGCTTT+TGG - Chr4:81087496-81087515 MsG0480023112.01.T01:CDS 45.0%
!!! GTTGAAGGGGTTGGTTTTGT+AGG - Chr4:81087535-81087554 MsG0480023112.01.T01:CDS 45.0%
!!! TGAAGGGGTTGGTTTTGTAG+GGG - Chr4:81087533-81087552 MsG0480023112.01.T01:CDS 45.0%
!!! TTTGTAGGGGCTGGTTTTGT+AGG - Chr4:81087520-81087539 MsG0480023112.01.T01:CDS 45.0%
CTAGCTAGTGATAGCCCACA+AGG + Chr4:81087916-81087935 MsG0480023113.01.T01:CDS 50.0%
TGATCAGACTCAGGCACACA+AGG + Chr4:81088164-81088183 MsG0480023113.01.T01:CDS 50.0%
TTAGGTGCCTTAGCAAGAGC+TGG - Chr4:81088111-81088130 None:intergenic 50.0%
!! GCTGGGACTAATGGTTTTGC+TGG - Chr4:81087571-81087590 MsG0480023112.01.T01:CDS 50.0%
!! GGTGCTGGAGCTTTAGCTTT+AGG - Chr4:81088090-81088109 None:intergenic 50.0%
TCCAGCACCAGCTCTTGCTA+AGG + Chr4:81088101-81088120 MsG0480023113.01.T01:CDS 55.0%
!! GTGCTGGAGCAGGTGTTGAA+GGG - Chr4:81087549-81087568 MsG0480023112.01.T01:CDS 55.0%
!! TGCTGGAGCAGGTGTTGAAG+GGG - Chr4:81087548-81087567 MsG0480023112.01.T01:CDS 55.0%
GCCTTAGCAAGAGCTGGTGC+TGG - Chr4:81088105-81088124 None:intergenic 60.0%
!! GGAGCAGGTGTTGAAGGGGT+TGG - Chr4:81087544-81087563 MsG0480023112.01.T01:CDS 60.0%
!! GGTGCTGGAGCAGGTGTTGA+AGG - Chr4:81087550-81087569 MsG0480023112.01.T01:CDS 60.0%
!! GGTTTTGCTGGTGCTGGAGC+AGG - Chr4:81087559-81087578 MsG0480023112.01.T01:CDS 60.0%
!!! GGGGCTGGTTTTGTAGGTGC+TGG - Chr4:81087514-81087533 MsG0480023112.01.T01:CDS 60.0%
!!! GGGGTTGGTTTTGTAGGGGC+TGG - Chr4:81087529-81087548 MsG0480023112.01.T01:CDS 60.0%
!!! GGTGCTTTTGGTGCTGCAGC+TGG - Chr4:81087484-81087503 MsG0480023112.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 81087409 81088287 81087409 ID=MsG0480023113.01;Name=MsG0480023113.01
Chr4 mRNA 81087409 81088287 81087409 ID=MsG0480023113.01.T01;Parent=MsG0480023113.01;Name=MsG0480023113.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|292
Chr4 exon 81087409 81088287 81087409 ID=MsG0480023113.01.T01:exon:11350;Parent=MsG0480023113.01.T01
Chr4 CDS 81087409 81088287 81087409 ID=MsG0480023113.01.T01:cds;Parent=MsG0480023113.01.T01
Gene Sequence

>MsG0480023113.01.T01

ATGAATCAAATTTTCTTGTTCTCACTCCTACTACTTGCCTCTATCTTCTCAATCACCACTTTAGCTCAATCACCAGCTGCAGCACCAAAAGCACCAGAAAAACCAGCACCTACAAAACCAGCCCCTACAAAACCAACCCCTTCAACACCTGCTCCAGCACCAGCAAAACCATTAGTCCCAGCATTACCACAATCACCTACAACAAATCCTGATTCGTCAGGAAACCAAGACATAATCAAGATTCTAAGAAAAGCCAAATCATTCAACACACTAATCCGTTTGCTCAAGACAACACAAATCATCAACCAAGTCAATGCACAGCTTGTAACTACAAAAAATGGAGGCTTGACAATTCTTGCACCAGATGATGGTGCCTTTTCAGAACTCAAAGCAGGTTACTTCAACTCCTTGGGAGAAAGACAACAAAAAGAATTGATTCAGTATCATGTTCTTCCTGAATATGTATCAAGTTCGAACTTTGATTCTCTCAGCAACCCTGTGTTGACACTAGCTAGTGATAGCCCACAAGGATTTCAGATTAATGTGACTGCATATGGAAACAGTGTGAATATTTCAACTGGCGTTGTTGATACAACCATCACTGGAATCGTTTACGCTGATAAAACACTTGCTATATATCATGTGAATAAGGTTCTTATTCCTTTGGATTTTGTTAAGCCTAAAGCTAAAGCTCCAGCACCAGCTCTTGCTAAGGCACCTAAAGCTGAGAAAGAGAATTCATCTGCAGATGATGATGATCAGACTCAGGCACACAAGGATAGTTCTGATGCCATTAGCTTGAATAACATGCATGGAATATCAGTGTTGGTTTCCCTTGGAATTTCTCTGCTTGCAGTTGCAGTAACAACTATGTTGTGA

Protein sequence

>MsG0480023113.01.T01

MNQIFLFSLLLLASIFSITTLAQSPAAAPKAPEKPAPTKPAPTKPTPSTPAPAPAKPLVPALPQSPTTNPDSSGNQDIIKILRKAKSFNTLIRLLKTTQIINQVNAQLVTTKNGGLTILAPDDGAFSELKAGYFNSLGERQQKELIQYHVLPEYVSSSNFDSLSNPVLTLASDSPQGFQINVTAYGNSVNISTGVVDTTITGIVYADKTLAIYHVNKVLIPLDFVKPKAKAPAPALAKAPKAEKENSSADDDDQTQAHKDSSDAISLNNMHGISVLVSLGISLLAVAVTTML*