AlfalfaGEDB | MsG0480020816.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020816.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020816.01.T01	KYP68511.1	65.432	81	19	5	1	75	115	192	4.30E-18	84.3

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020816.01.T01	A0A151TNA5	65.432	81	19	5	1	75	115	192	2.05e-18	84.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280010892.01	MsG0480020816.01	0.922772	6.359966e-89	2.452734e-84
MsG0280010893.01	MsG0480020816.01	0.846916	1.607542e-59	2.809813e-56
MsG0480020552.01	MsG0480020816.01	0.878165	3.500035e-69	1.808826e-65
MsG0480020815.01	MsG0480020816.01	0.918168	2.172128e-86	6.522692e-82
MsG0480020816.01	MsG0480020817.01	0.881685	1.954418e-70	1.156097e-66
MsG0480020816.01	MsG0480020818.01	0.891486	3.803558e-74	3.341600e-70
MsG0480020816.01	MsG0480023097.01	0.811509	6.755984e-51	4.190032e-48
MsG0480020816.01	MsG0480023113.01	0.891539	3.622301e-74	3.188868e-70
MsG0480020816.01	MsG0480023114.01	0.863646	2.145448e-64	6.551765e-61
MsG0480020816.01	MsG0480023115.01	0.903471	3.352023e-79	4.969573e-75
MsG0480020816.01	MsG0480023116.01	0.917106	7.948627e-86	2.255393e-81
MsG0480020816.01	MsG0880044994.01	0.888329	6.505265e-73	5.017880e-69
MsG0480020816.01	MsG0880045339.01	0.891464	3.880702e-74	3.406249e-70
MsG0480020816.01	MsG0880047773.01	0.818613	1.788898e-52	1.345467e-49
MsG0280006934.01	MsG0480020816.01	0.803431	3.497854e-49	1.757424e-46
MsG0280009327.01	MsG0480020816.01	0.877252	7.294741e-69	3.643445e-65

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020816.01.T01	MTR_4g059780	93.421	76	4	1	1	75	228	303	5.56e-21	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 16 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TACCTTGATCCTGATCATTT+TGG	0.263017	4:-48820035	None:intergenic
GGCTCCTAAAGCTGCTAATA+AGG	0.272377	4:+48819987	MsG0480020816.01.T01:CDS
CGCTACTAAGGCACCTGCTT+TGG	0.312281	4:+48819939	MsG0480020816.01.T01:CDS
TTCTCCTTATTAGCAGCTTT+AGG	0.349370	4:-48819991	None:intergenic
GTGAGATTGTGTGGAATTCT+TGG	0.371931	4:+48820069	MsG0480020816.01.T01:CDS
GATCAGGATCAAGGTAAATC+TGG	0.376467	4:+48820042	MsG0480020816.01.T01:CDS
AACTCGTACTGCTGCTGCTA+AGG	0.455742	4:+48819966	MsG0480020816.01.T01:CDS
TCGTGATTTCTTCGCTACTA+AGG	0.488348	4:+48819927	MsG0480020816.01.T01:CDS
TGATCAAGACCAAAATGATC+AGG	0.490187	4:+48820026	MsG0480020816.01.T01:CDS
GACCAAAATGATCAGGATCA+AGG	0.527131	4:+48820033	MsG0480020816.01.T01:CDS
GTACGAGTTGGAGCCAAAGC+AGG	0.540425	4:-48819952	None:intergenic
TTAGCAGCAGCAGTACGAGT+TGG	0.559502	4:-48819964	None:intergenic
TTGTTGCTGTAGCAATGATG+TGG	0.582648	4:+48820109	MsG0480020816.01.T01:CDS
TCTGGAGCAGTGAGATTGTG+TGG	0.636283	4:+48820060	MsG0480020816.01.T01:CDS
TGGAATTCTTGGAACAACGT+TGG	0.689897	4:+48820080	MsG0480020816.01.T01:CDS
TTAGTAGCGAAGAAATCACG+AGG	0.758452	4:-48819925	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TGATCAAGACCAAAATGATC+AGG	+	Chr4:48820026-48820045	MsG0480020816.01.T01:CDS	35.0%
	TTCTCCTTATTAGCAGCTTT+AGG	-	Chr4:48819994-48820013	None:intergenic	35.0%
!	TACCTTGATCCTGATCATTT+TGG	-	Chr4:48820038-48820057	None:intergenic	35.0%
	GACCAAAATGATCAGGATCA+AGG	+	Chr4:48820033-48820052	MsG0480020816.01.T01:CDS	40.0%
	GATCAGGATCAAGGTAAATC+TGG	+	Chr4:48820042-48820061	MsG0480020816.01.T01:CDS	40.0%
	GTGAGATTGTGTGGAATTCT+TGG	+	Chr4:48820069-48820088	MsG0480020816.01.T01:CDS	40.0%
	TCGTGATTTCTTCGCTACTA+AGG	+	Chr4:48819927-48819946	MsG0480020816.01.T01:CDS	40.0%
	TGGAATTCTTGGAACAACGT+TGG	+	Chr4:48820080-48820099	MsG0480020816.01.T01:CDS	40.0%
	TTAGTAGCGAAGAAATCACG+AGG	-	Chr4:48819928-48819947	None:intergenic	40.0%
	TTGTTGCTGTAGCAATGATG+TGG	+	Chr4:48820109-48820128	MsG0480020816.01.T01:CDS	40.0%
	GGCTCCTAAAGCTGCTAATA+AGG	+	Chr4:48819987-48820006	MsG0480020816.01.T01:CDS	45.0%
	AACTCGTACTGCTGCTGCTA+AGG	+	Chr4:48819966-48819985	MsG0480020816.01.T01:CDS	50.0%
	TTAGCAGCAGCAGTACGAGT+TGG	-	Chr4:48819967-48819986	None:intergenic	50.0%
!	TCTGGAGCAGTGAGATTGTG+TGG	+	Chr4:48820060-48820079	MsG0480020816.01.T01:CDS	50.0%
!	CGCTACTAAGGCACCTGCTT+TGG	+	Chr4:48819939-48819958	MsG0480020816.01.T01:CDS	55.0%
!	GTACGAGTTGGAGCCAAAGC+AGG	-	Chr4:48819955-48819974	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	48819910	48820137	48819910	ID=MsG0480020816.01;Name=MsG0480020816.01
Chr4	mRNA	48819910	48820137	48819910	ID=MsG0480020816.01.T01;Parent=MsG0480020816.01;Name=MsG0480020816.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|75
Chr4	exon	48819910	48820137	48819910	ID=MsG0480020816.01.T01:exon:657;Parent=MsG0480020816.01.T01
Chr4	CDS	48819910	48820137	48819910	ID=MsG0480020816.01.T01:cds;Parent=MsG0480020816.01.T01

Gene Sequence	>MsG0480020816.01.T01 TTGGATAAAGTGCTTCCTCGTGATTTCTTCGCTACTAAGGCACCTGCTTTGGCTCCAACTCGTACTGCTGCTGCTAAGGCTCCTAAAGCTGCTAATAAGGAGAATTCTTCTGCTGATGATCAAGACCAAAATGATCAGGATCAAGGTAAATCTGGAGCAGTGAGATTGTGTGGAATTCTTGGAACAACGTTGGTGTCTTTTGTTGCTGTAGCAATGATGTGGAGTTGA
Protein sequence	>MsG0480020816.01.T01 LDKVLPRDFFATKAPALAPTRTAAAKAPKAANKENSSADDQDQNDQDQGKSGAVRLCGILGTTLVSFVAVAMMWS*

Copyright © Liu Lab , TPR, XTBG, China.

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