AlfalfaGEDB | MsG0380014010.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014010.01

KEGG: K02965
GO: GO:0003723 GO:0003735 GO:0005840 GO:0006412 GO:0015935
PFAM: PF00203

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014010.01.T02	YP_009657390.1	100	49	0	0	1	49	44	92	3.71E-28	105
MsG0380014010.01.T01	YP_009141648.1	100	92	0	0	1	92	1	92	4.20E-60	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014010.01.T01	A4GGF9	92.391	92	7	1	92	1	92	1.36E-58	177
MsG0380014010.01.T02	A4GGF9	97.959	49	1	1	49	44	92	3.67E-30	103
MsG0380014010.01.T03	P07816	96.296	27	1	1	27	66	92	8.98E-13	58.5

Trembl:

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014010.01.T01	A0A4P8F4F6	100.000	92	1	92	1	92	2.01e-60	188
MsG0380014010.01.T02	A0A4P8F461	100.000	49	1	49	44	92	1.77e-28	105
MsG0380014010.01.T03	A0A898NBR1	100.000	27	1	27	66	92	6.13e-11	60.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0380013927.01	MsG0380014010.01	0.802981	4.333622e-49	2.152308e-46
MsG0380013939.01	MsG0380014010.01	0.827484	1.525246e-54	1.474498e-51
MsG0380013958.01	MsG0380014010.01	0.817424	3.322318e-52	2.418637e-49
MsG0380013965.01	MsG0380014010.01	0.800596	1.338235e-48	6.256154e-46
MsG0380013979.01	MsG0380014010.01	0.802471	5.523919e-49	2.708140e-46
MsG0380013980.01	MsG0380014010.01	0.809799	1.582557e-50	9.381342e-48
MsG0380013988.01	MsG0380014010.01	0.814559	1.448785e-51	9.753173e-49
MsG0380013996.01	MsG0380014010.01	0.800053	1.726071e-48	7.958905e-46
MsG0380014009.01	MsG0380014010.01	0.821373	4.179009e-53	3.393676e-50
MsG0380014010.01	MsG0380014012.01	0.804329	2.275733e-49	1.170088e-46
MsG0380014010.01	MsG0380014013.01	0.812913	3.337786e-51	2.148854e-48
MsG0380014010.01	MsG0380014037.01	0.835399	1.716117e-56	2.096246e-53
MsG0380014010.01	MsG0380014038.01	0.803656	3.140643e-49	1.587092e-46
MsG0380014010.01	MsG0380014041.01	0.819206	1.312042e-52	1.003134e-49
MsG0380014010.01	MsG0380014042.01	0.946000	1.060440e-104	1.687144e-99
MsG0380014010.01	MsG0380014055.01	0.823189	1.583675e-53	1.354049e-50
MsG0380014010.01	MsG0380014056.01	0.929405	7.288835e-93	4.060933e-88
MsG0280007041.01	MsG0380014010.01	0.803021	4.252921e-49	2.114311e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014010.01.T02	MTR_4g051420	97.959	49	1	0	1	49	44	92	4.59e-31	103
MsG0380014010.01.T02	MTR_3g035720	97.959	49	1	0	1	49	44	92	4.59e-31	103
MsG0380014010.01.T02	MTR_8g038480	89.474	38	4	0	12	49	43	80	6.96e-21	77.0
MsG0380014010.01.T02	MTR_0002s0800	68.627	51	14	1	1	49	166	216	3.29e-18	73.9
MsG0380014010.01.T02	MTR_0002s0360	68.627	51	14	1	1	49	166	216	3.29e-18	73.9
MsG0380014010.01.T03	MTR_4g051420	96.296	27	1	0	1	27	66	92	1.46e-13	57.8
MsG0380014010.01.T03	MTR_3g035720	96.296	27	1	0	1	27	66	92	1.46e-13	57.8
MsG0380014010.01.T03	MTR_8g038480	92.593	27	2	0	1	27	54	80	6.45e-13	55.8
MsG0380014010.01.T01	MTR_4g051420	98.913	92	1	0	1	92	1	92	3.93e-63	186
MsG0380014010.01.T01	MTR_3g035720	98.913	92	1	0	1	92	1	92	3.93e-63	186
MsG0380014010.01.T01	MTR_0002s0800	62.245	98	30	3	2	92	119	216	5.06e-34	116
MsG0380014010.01.T01	MTR_0002s0360	62.245	98	30	3	2	92	119	216	5.06e-34	116
MsG0380014010.01.T01	MTR_8g038480	89.474	38	4	0	55	92	43	80	2.08e-20	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014010.01.T02	ATCG00820	87.755	49	6	0	1	49	44	92	2.64e-28	96.3
MsG0380014010.01.T03	ATCG00820	92.593	27	2	0	1	27	66	92	1.46e-13	58.2
MsG0380014010.01.T01	ATCG00820	85.870	92	13	0	1	92	1	92	3.38e-55	166
MsG0380014010.01.T01	AT5G47320	44.186	86	40	2	1	85	132	210	8.66e-14	63.9

Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAAATTCTCCCAATTTA+TGG	0.137320	3:+48641322	None:intergenic
ATACGATCTGTTATATAAAT+AGG	0.289134	3:+48641351	None:intergenic
TCGTATGGTAGGCCATAAAT+TGG	0.313687	3:-48641334	MsG0380014010.01.T01:CDS
AGGTATAATGGTAGATGTTC+TGG	0.324700	3:+48641422	None:intergenic
CCCAATCATTGTAGGTATAA+TGG	0.326942	3:+48641410	None:intergenic
TTTCACCTACTCTAAACTTC+CGG	0.337945	3:-48641305	MsG0380014010.01.T01:CDS
ACCATTATACCTACAATGAT+TGG	0.350564	3:-48641411	MsG0380014010.01.T01:CDS
CATACCATTGCTATTCATAA+TGG	0.354095	3:-48641387	MsG0380014010.01.T01:CDS
TTCACCTACTCTAAACTTCC+GGG	0.355100	3:-48641304	MsG0380014010.01.T01:CDS
AATAAATGATTCGCTACAAA+AGG	0.410516	3:+48641501	None:intergenic
ATGGTATGCCCAATCATTGT+AGG	0.463349	3:+48641402	None:intergenic
ATAAATAAGCTTAACACAAA+AGG	0.463559	3:-48641471	MsG0380014010.01.T01:CDS
CCATTATACCTACAATGATT+GGG	0.487902	3:-48641410	MsG0380014010.01.T01:CDS
CGTATGGTAGGCCATAAATT+GGG	0.512944	3:-48641333	MsG0380014010.01.T01:CDS
TATATAACAGATCGTATGGT+AGG	0.535301	3:-48641345	MsG0380014010.01.T01:CDS
TATTTATATAACAGATCGTA+TGG	0.571298	3:-48641349	MsG0380014010.01.T01:CDS
CACCTACTCTAAACTTCCGG+GGG	0.635563	3:-48641302	MsG0380014010.01.T01:CDS
TCACCTACTCTAAACTTCCG+GGG	0.640846	3:-48641303	MsG0380014010.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGAAATAATAATAACT+TGG	-	Chr3:48641348-48641367	MsG0380014010.01.T01:CDS	10.0%
!!	ATAAATAAGCTTAACACAAA+AGG	-	Chr3:48641323-48641342	MsG0380014010.01.T01:CDS	20.0%
!!	ATACGATCTGTTATATAAAT+AGG	+	Chr3:48641446-48641465	None:intergenic	20.0%
!!	TATTTATATAACAGATCGTA+TGG	-	Chr3:48641445-48641464	MsG0380014010.01.T01:CDS	20.0%
!	AATAAATGATTCGCTACAAA+AGG	+	Chr3:48641296-48641315	None:intergenic	25.0%
!!	TTTTCCATTATGAATAGCAA+TGG	+	Chr3:48641414-48641433	None:intergenic	25.0%
	ACCATTATACCTACAATGAT+TGG	-	Chr3:48641383-48641402	MsG0380014010.01.T01:CDS	30.0%
	CCATTATACCTACAATGATT+GGG	-	Chr3:48641384-48641403	MsG0380014010.01.T01:CDS	30.0%
	GTGAAAATTCTCCCAATTTA+TGG	+	Chr3:48641475-48641494	None:intergenic	30.0%
	TATATAACAGATCGTATGGT+AGG	-	Chr3:48641449-48641468	MsG0380014010.01.T01:CDS	30.0%
!	CATACCATTGCTATTCATAA+TGG	-	Chr3:48641407-48641426	MsG0380014010.01.T01:CDS	30.0%
	AGGTATAATGGTAGATGTTC+TGG	+	Chr3:48641375-48641394	None:intergenic	35.0%
	CCCAATCATTGTAGGTATAA+TGG	+	Chr3:48641387-48641406	None:intergenic	35.0%
	TTTCACCTACTCTAAACTTC+CGG	-	Chr3:48641489-48641508	MsG0380014010.01.T01:CDS	35.0%
	ATGGTATGCCCAATCATTGT+AGG	+	Chr3:48641395-48641414	None:intergenic	40.0%
	CGTATGGTAGGCCATAAATT+GGG	-	Chr3:48641461-48641480	MsG0380014010.01.T01:CDS	40.0%
	TCGTATGGTAGGCCATAAAT+TGG	-	Chr3:48641460-48641479	MsG0380014010.01.T01:CDS	40.0%
	TTCACCTACTCTAAACTTCC+GGG	-	Chr3:48641490-48641509	MsG0380014010.01.T01:CDS	40.0%
!!	TATCATTTTTCGCATACCCC+CGG	+	Chr3:48641511-48641530	None:intergenic	40.0%
	TCACCTACTCTAAACTTCCG+GGG	-	Chr3:48641491-48641510	MsG0380014010.01.T01:CDS	45.0%
	CACCTACTCTAAACTTCCGG+GGG	-	Chr3:48641492-48641511	MsG0380014010.01.T01:CDS	50.0%
!	TACCCCCGGAAGTTTAGAGT+AGG	+	Chr3:48641497-48641516	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48641269	48641547	48641269	ID=MsG0380014010.01;Name=MsG0380014010.01
Chr3	mRNA	48641269	48641547	48641269	ID=MsG0380014010.01.T01;Parent=MsG0380014010.01;Name=MsG0380014010.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|92
Chr3	exon	48641269	48641547	48641269	ID=MsG0380014010.01.T01:exon:9389;Parent=MsG0380014010.01.T01
Chr3	CDS	48641269	48641547	48641269	ID=MsG0380014010.01.T01:cds;Parent=MsG0380014010.01.T01
Chr3	mRNA	48641269	48641418	48641269	ID=MsG0380014010.01.T02;Parent=MsG0380014010.01;Name=MsG0380014010.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|49
Chr3	exon	48641269	48641418	48641269	ID=MsG0380014010.01.T02:exon:9390;Parent=MsG0380014010.01.T02
Chr3	CDS	48641269	48641418	48641269	ID=MsG0380014010.01.T02:cds;Parent=MsG0380014010.01.T02
Chr3	mRNA	48641269	48641352	48641269	ID=MsG0380014010.01.T03;Parent=MsG0380014010.01;Name=MsG0380014010.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|27
Chr3	exon	48641269	48641352	48641269	ID=MsG0380014010.01.T03:exon:9391;Parent=MsG0380014010.01.T03
Chr3	CDS	48641269	48641352	48641269	ID=MsG0380014010.01.T03:cds;Parent=MsG0380014010.01.T03

Gene Sequence

>MsG0380014010.01.T02

ATGATTGGGCATACCATTGCTATTCATAATGGAAAAGAGCATTTACCTATTTATATAACAGATCGTATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

>MsG0380014010.01.T03

ATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

>MsG0380014010.01.T01

ATGACACGTTCACGAAAAAAAAATCCTTTTGTAGCGAATCATTTATTAAAAAAAATAAATAAGCTTAACACAAAAGGAGAAAAAGAAATAATAATAACTTGGTCCAGAACATCTACCATTATACCTACAATGATTGGGCATACCATTGCTATTCATAATGGAAAAGAGCATTTACCTATTTATATAACAGATCGTATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

Protein sequence

>MsG0380014010.01.T02

MIGHTIAIHNGKEHLPIYITDRMVGHKLGEFSPTLNFRGYAKNDNRSRR*

>MsG0380014010.01.T03

MVGHKLGEFSPTLNFRGYAKNDNRSRR*

>MsG0380014010.01.T01

MTRSRKKNPFVANHLLKKINKLNTKGEKEIIITWSRTSTIIPTMIGHTIAIHNGKEHLPIYITDRMVGHKLGEFSPTLNFRGYAKNDNRSRR*