AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013979.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013979.01.T01 MTR_4g051220 96.774 93 3 0 1 93 100 192 3.26e-62 189
MsG0380013979.01.T01 MTR_0002s0590 66.265 83 28 0 1 83 100 182 7.13e-34 121
MsG0380013979.01.T01 MTR_0477s0040 100.000 42 0 0 99 140 1 42 1.01e-23 87.8
MsG0380013979.01.T01 MTR_4g051213 100.000 42 0 0 99 140 1 42 1.01e-23 87.8
MsG0380013979.01.T01 MTR_8g102790 91.892 37 3 0 99 135 1 37 4.52e-18 73.2
MsG0380013979.01.T01 MTR_1g041800 67.857 56 6 1 99 142 1 56 3.57e-17 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013979.01.T01 ATCG00380 79.570 93 19 0 1 93 100 192 8.05e-47 150

Find 30 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAATAATAGATAGTAAA+TGG 0.189787 3:-48556133 MsG0380013979.01.T01:CDS
CGAATAATTCCGACAACTTC+TGG 0.195705 3:-48555431 MsG0380013979.01.T01:CDS
ATGACTAGATCTCGGATAAA+TGG 0.278088 3:-48555500 MsG0380013979.01.T01:CDS
GATTGACTAATCCTTTATAT+TGG 0.284280 3:+48556153 None:intergenic
CTTGATCCATTCCAATATAA+AGG 0.291752 3:-48556164 MsG0380013979.01.T01:CDS
AATTATTCGTAATAAGATAT+TGG 0.314059 3:+48555445 None:intergenic
TTTGAAAATTAATGAATTGC+TGG 0.365999 3:-48556105 MsG0380013979.01.T01:intron
ATGGATTGGCAATTCTTCGC+GGG 0.450528 3:+48556193 None:intergenic
TTTATCCGAGATCTAGTCAT+AGG 0.463215 3:+48555503 None:intergenic
TGAGGTTTGCAACGATAACT+TGG 0.468099 3:+48556284 None:intergenic
ACTAATCCTTTATATTGGAA+TGG 0.475526 3:+48556158 None:intergenic
GTCTGTGGTTAACTAATTGA+CGG 0.477609 3:+48556339 None:intergenic
TCAAATAATAGATAGTAAAT+GGG 0.487910 3:-48556132 MsG0380013979.01.T01:CDS
ATAATAGATAGTAAATGGGT+TGG 0.504615 3:-48556128 MsG0380013979.01.T01:CDS
GATATTGGCTACAATGGTAA+AGG 0.509030 3:+48555460 None:intergenic
GGATCAAGAGTCAAATGGAT+TGG 0.511159 3:+48556179 None:intergenic
TCTGTGGTTAACTAATTGAC+GGG 0.521261 3:+48556340 None:intergenic
AATGGATTGGCAATTCTTCG+CGG 0.545268 3:+48556192 None:intergenic
TCAAGATATTATTACAGCGA+AGG 0.559737 3:-48556264 MsG0380013979.01.T01:CDS
TTCGCTGTAATAATATCTTG+AGG 0.560954 3:+48556266 None:intergenic
TAATAAGATATTGGCTACAA+TGG 0.565840 3:+48555454 None:intergenic
CAAGATATTATTACAGCGAA+GGG 0.569002 3:-48556263 MsG0380013979.01.T01:CDS
TGCTACCTATGACTAGATCT+CGG 0.572595 3:-48555508 MsG0380013979.01.T01:CDS
GCATTCACTTATTACAGAGA+TGG 0.579208 3:-48555398 MsG0380013979.01.T01:CDS
TTAACTAATTGACGGGCTTG+AGG 0.581554 3:+48556347 None:intergenic
GGAATGGATCAAGAGTCAAA+TGG 0.582974 3:+48556174 None:intergenic
GATTGGCAATTCTTCGCGGG+GGG 0.584547 3:+48556196 None:intergenic
TGGATTGGCAATTCTTCGCG+GGG 0.610754 3:+48556194 None:intergenic
CATTAACTAAAACATGTCTG+TGG 0.663264 3:+48556324 None:intergenic
GGATTGGCAATTCTTCGCGG+GGG 0.681198 3:+48556195 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATCAAATAATTTATCTTA+CGG + Chr3:48556116-48556135 None:intergenic 10.0%
!! AATTATTCGTAATAAGATAT+TGG + Chr3:48556308-48556327 None:intergenic 15.0%
!! ATCAAATAATAGATAGTAAA+TGG - Chr3:48555617-48555636 MsG0380013979.01.T01:intron 15.0%
!! TCAAATAATAGATAGTAAAT+GGG - Chr3:48555618-48555637 MsG0380013979.01.T01:intron 15.0%
!!! TAAACAATGAAATTTTAGAA+TGG - Chr3:48556217-48556236 MsG0380013979.01.T01:CDS 15.0%
!! GTTTATTACAGAAAGAAATT+AGG - Chr3:48555812-48555831 MsG0380013979.01.T01:intron 20.0%
!! TTCTGTAATAAACAAAATAG+TGG + Chr3:48555805-48555824 None:intergenic 20.0%
!! TTTGAAAATTAATGAATTGC+TGG - Chr3:48555645-48555664 MsG0380013979.01.T01:intron 20.0%
! AACATTGATGAACTTGATAA+GGG - Chr3:48555895-48555914 MsG0380013979.01.T01:intron 25.0%
! ACTAATCCTTTATATTGGAA+TGG + Chr3:48555595-48555614 None:intergenic 25.0%
! AGTACGAAATAACGTAAAAA+TGG + Chr3:48556154-48556173 None:intergenic 25.0%
! ATAAAATAATCCCCAAACAA+AGG + Chr3:48556190-48556209 None:intergenic 25.0%
! ATAATAGATAGTAAATGGGT+TGG - Chr3:48555622-48555641 MsG0380013979.01.T01:intron 25.0%
! GATTGACTAATCCTTTATAT+TGG + Chr3:48555600-48555619 None:intergenic 25.0%
! TAACATTGATGAACTTGATA+AGG - Chr3:48555894-48555913 MsG0380013979.01.T01:intron 25.0%
! TATTGTACAATTCCTTTGTT+TGG - Chr3:48556175-48556194 MsG0380013979.01.T01:CDS 25.0%
!! AACAAAATAGTGGAATAAAG+TGG + Chr3:48555795-48555814 None:intergenic 25.0%
!! ACAAAATAGTGGAATAAAGT+GGG + Chr3:48555794-48555813 None:intergenic 25.0%
!! ATTGTACAATTCCTTTGTTT+GGG - Chr3:48556176-48556195 MsG0380013979.01.T01:CDS 25.0%
!! TAATAAGATATTGGCTACAA+TGG + Chr3:48556299-48556318 None:intergenic 25.0%
!!! TTAAGAATAATTTTGATCCG+AGG - Chr3:48555716-48555735 MsG0380013979.01.T01:intron 25.0%
CAAATAATTTATCTTACGGC+AGG + Chr3:48556112-48556131 None:intergenic 30.0%
CAAGATATTATTACAGCGAA+GGG - Chr3:48555487-48555506 MsG0380013979.01.T01:CDS 30.0%
CATTAACTAAAACATGTCTG+TGG + Chr3:48555429-48555448 None:intergenic 30.0%
CTTGATCCATTCCAATATAA+AGG - Chr3:48555586-48555605 MsG0380013979.01.T01:intron 30.0%
TAATCATTATGTAGGAGAGA+TGG + Chr3:48556001-48556020 None:intergenic 30.0%
TCAAGATATTATTACAGCGA+AGG - Chr3:48555486-48555505 MsG0380013979.01.T01:CDS 30.0%
TCATTATTCCAACTTTGATG+TGG + Chr3:48555848-48555867 None:intergenic 30.0%
TTCGCTGTAATAATATCTTG+AGG + Chr3:48555487-48555506 None:intergenic 30.0%
! TTGTACAATTCCTTTGTTTG+GGG - Chr3:48556177-48556196 MsG0380013979.01.T01:CDS 30.0%
!!! CACAGACATGTTTTAGTTAA+TGG - Chr3:48555427-48555446 MsG0380013979.01.T01:CDS 30.0%
ATGACTAGATCTCGGATAAA+TGG - Chr3:48556250-48556269 MsG0380013979.01.T01:CDS 35.0%
CATACATATTCCATTTGCGT+AGG - Chr3:48556043-48556062 MsG0380013979.01.T01:intron 35.0%
GAGCAAAAAAACTTACTTCG+AGG - Chr3:48556086-48556105 MsG0380013979.01.T01:intron 35.0%
GCATTCACTTATTACAGAGA+TGG - Chr3:48556352-48556371 MsG0380013979.01.T01:CDS 35.0%
GTCTGTGGTTAACTAATTGA+CGG + Chr3:48555414-48555433 None:intergenic 35.0%
TAGAGAATCCACATCAAAGT+TGG - Chr3:48555837-48555856 MsG0380013979.01.T01:intron 35.0%
TCTGTGGTTAACTAATTGAC+GGG + Chr3:48555413-48555432 None:intergenic 35.0%
TTTATCCGAGATCTAGTCAT+AGG + Chr3:48556250-48556269 None:intergenic 35.0%
! GATATTGGCTACAATGGTAA+AGG + Chr3:48556293-48556312 None:intergenic 35.0%
! TCTTTTTCTCCAGAAGTTGT+CGG + Chr3:48556331-48556350 None:intergenic 35.0%
ACCCACGTCTATACATGAAT+GGG + Chr3:48555749-48555768 None:intergenic 40.0%
CGAATAATTCCGACAACTTC+TGG - Chr3:48556319-48556338 MsG0380013979.01.T01:CDS 40.0%
GGAATGGATCAAGAGTCAAA+TGG + Chr3:48555579-48555598 None:intergenic 40.0%
GGATCAAGAGTCAAATGGAT+TGG + Chr3:48555574-48555593 None:intergenic 40.0%
TACCCACGTCTATACATGAA+TGG + Chr3:48555750-48555769 None:intergenic 40.0%
TCATGTATAGACGTGGGTAT+AGG - Chr3:48555751-48555770 MsG0380013979.01.T01:intron 40.0%
TGAGGTTTGCAACGATAACT+TGG + Chr3:48555469-48555488 None:intergenic 40.0%
TGATGAACTTGATAAGGGTG+CGG - Chr3:48555900-48555919 MsG0380013979.01.T01:intron 40.0%
TGCTACCTATGACTAGATCT+CGG - Chr3:48556242-48556261 MsG0380013979.01.T01:CDS 40.0%
! ATAGTGGAATAAAGTGGGCA+AGG + Chr3:48555789-48555808 None:intergenic 40.0%
! CGAGGGCATAATCATTATGT+AGG + Chr3:48556009-48556028 None:intergenic 40.0%
! GACAACTTCTGGAGAAAAAG+AGG - Chr3:48556330-48556349 MsG0380013979.01.T01:CDS 40.0%
!! AATGGATTGGCAATTCTTCG+CGG + Chr3:48555561-48555580 None:intergenic 40.0%
!! GTAGGCTTTTTGTTTACCGA+GGG + Chr3:48555943-48555962 None:intergenic 40.0%
!! TGTAGGCTTTTTGTTTACCG+AGG + Chr3:48555944-48555963 None:intergenic 40.0%
!! TTAACTAATTGACGGGCTTG+AGG + Chr3:48555406-48555425 None:intergenic 40.0%
ATGCCTCACTATTCACTCGA+GGG + Chr3:48556026-48556045 None:intergenic 45.0%
ATTATGTGCGCCTACGCAAA+TGG + Chr3:48556056-48556075 None:intergenic 45.0%
CATGAATGGGGGAAAATCCT+CGG + Chr3:48555736-48555755 None:intergenic 45.0%
CCACGTCTATACATGAATGG+GGG + Chr3:48555747-48555766 None:intergenic 45.0%
CCCACGTCTATACATGAATG+GGG + Chr3:48555748-48555767 None:intergenic 45.0%
CCCCATTCATGTATAGACGT+GGG - Chr3:48555745-48555764 MsG0380013979.01.T01:intron 45.0%
TATGCCTCACTATTCACTCG+AGG + Chr3:48556027-48556046 None:intergenic 45.0%
! TAGCACTGGAACTGCTATGT+AGG + Chr3:48555961-48555980 None:intergenic 45.0%
!! ATGGATTGGCAATTCTTCGC+GGG + Chr3:48555560-48555579 None:intergenic 45.0%
!!! CTTAATTTAATATTTTAATT+AGG + Chr3:48555701-48555720 None:intergenic 5.0%
AAGAGAGGGATTCGAACCCT+CGG - Chr3:48555924-48555943 MsG0380013979.01.T01:intron 50.0%
ATGCCCTCGAGTGAATAGTG+AGG - Chr3:48556020-48556039 MsG0380013979.01.T01:intron 50.0%
CCCCCATTCATGTATAGACG+TGG - Chr3:48555744-48555763 MsG0380013979.01.T01:intron 50.0%
! GATAAGGGTGCGGAAAGAGA+GGG - Chr3:48555910-48555929 MsG0380013979.01.T01:intron 50.0%
! TGATAAGGGTGCGGAAAGAG+AGG - Chr3:48555909-48555928 MsG0380013979.01.T01:intron 50.0%
!! TGGATTGGCAATTCTTCGCG+GGG + Chr3:48555559-48555578 None:intergenic 50.0%
AGAGATGGCCGAGTGGTTGA+AGG + Chr3:48555986-48556005 None:intergenic 55.0%
ATGTAGGAGAGATGGCCGAG+TGG + Chr3:48555993-48556012 None:intergenic 55.0%
GATTGGCAATTCTTCGCGGG+GGG + Chr3:48555557-48555576 None:intergenic 55.0%
GTGCTACGCCTTCAACCACT+CGG - Chr3:48555975-48555994 MsG0380013979.01.T01:intron 55.0%
! AGTGGTTGAAGGCGTAGCAC+TGG + Chr3:48555975-48555994 None:intergenic 55.0%
! GGATTGGCAATTCTTCGCGG+GGG + Chr3:48555558-48555577 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr3 gene 48555388 48556384 48555388 ID=MsG0380013979.01;Name=MsG0380013979.01
Chr3 mRNA 48555388 48556384 48555388 ID=MsG0380013979.01.T01;Parent=MsG0380013979.01;Name=MsG0380013979.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|142
Chr3 exon 48556106 48556384 48556106 ID=MsG0380013979.01.T01:exon:9346;Parent=MsG0380013979.01.T01
Chr3 exon 48555388 48555537 48555388 ID=MsG0380013979.01.T01:exon:9345;Parent=MsG0380013979.01.T01
Chr3 CDS 48556106 48556384 48556106 ID=MsG0380013979.01.T01:cds;Parent=MsG0380013979.01.T01
Chr3 CDS 48555388 48555537 48555388 ID=MsG0380013979.01.T01:cds;Parent=MsG0380013979.01.T01
Gene Sequence

>MsG0380013979.01.T01

ATGGCTTCGACTATTCCTCAAGCCCGTCAATTAGTTAACCACAGACATGTTTTAGTTAATGGTCGTATAGTAGATATACCAAGTTATCGTTGCAAACCTCAAGATATTATTACAGCGAAGGGTGAACAAAAATCTAGAACTCTGATTCAAAATTCTCTTGAATCAGCCCCCCGCGAAGAATTGCCAATCCATTTGACTCTTGATCCATTCCAATATAAAGGATTAGTCAATCAAATAATAGATAGTAAATGGGTTGGTTTGAAAATTAATGAATTGCTGAATGGATTGCTACCTATGACTAGATCTCGGATAAATGGAAATTTTATTGATAAGACCTTTACCATTGTAGCCAATATCTTATTACGAATAATTCCGACAACTTCTGGAGAAAAAGAGGCATTCACTTATTACAGAGATGGTGCGATTTGA

Protein sequence

>MsG0380013979.01.T01

MASTIPQARQLVNHRHVLVNGRIVDIPSYRCKPQDIITAKGEQKSRTLIQNSLESAPREELPIHLTLDPFQYKGLVNQIIDSKWVGLKINELLNGLLPMTRSRINGNFIDKTFTIVANILLRIIPTTSGEKEAFTYYRDGAI*