AlfalfaGEDB | MsG0380014042.01

M. sativa cultivar ZhongmuNo.1 / MsG0380014042.01

KEGG: K02965
GO: GO:0003723 GO:0003735 GO:0005840 GO:0006412 GO:0015935
PFAM: PF00203

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014042.01.T02	YP_009657390.1	100	49	0	0	1	49	44	92	3.71E-28	105
MsG0380014042.01.T01	YP_009141648.1	100	92	0	0	1	92	1	92	4.20E-60	188

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014042.01.T01	A4GGF9	92.391	92	7	1	92	1	92	1.36E-58	177
MsG0380014042.01.T02	A4GGF9	97.959	49	1	1	49	44	92	3.67E-30	103
MsG0380014042.01.T03	P07816	96.296	27	1	1	27	66	92	8.98E-13	58.5

Trembl:

Query id	Subject id	identity %	alignment length	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014042.01.T01	A0A4P8F4F6	100.000	92	1	92	1	92	2.01e-60	188
MsG0380014042.01.T02	A0A4P8F461	100.000	49	1	49	44	92	1.77e-28	105
MsG0380014042.01.T03	A0A898NBR1	100.000	27	1	27	66	92	6.13e-11	60.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049078.01	MsG0380014042.01	0.829628	4.628447e-55	4.763449e-52
MsG0080049079.01	MsG0380014042.01	0.814968	1.175847e-51	8.003257e-49
MsG0080049080.01	MsG0380014042.01	0.826428	2.727075e-54	2.556899e-51
MsG0380013927.01	MsG0380014042.01	0.826739	2.299945e-54	2.175451e-51
MsG0380013930.01	MsG0380014042.01	0.813328	2.705530e-51	1.761568e-48
MsG0380013939.01	MsG0380014042.01	0.831260	1.846034e-55	1.992552e-52
MsG0380013955.01	MsG0380014042.01	0.821453	4.006058e-53	3.260404e-50
MsG0380013958.01	MsG0380014042.01	0.836988	6.775666e-57	8.681585e-54
MsG0380013960.01	MsG0380014042.01	0.803003	4.290051e-49	2.131794e-46
MsG0380013965.01	MsG0380014042.01	0.803101	4.093653e-49	2.039314e-46
MsG0380013970.01	MsG0380014042.01	0.830453	2.912402e-55	3.069456e-52
MsG0380013979.01	MsG0380014042.01	0.814115	1.815567e-51	1.207587e-48
MsG0380013980.01	MsG0380014042.01	0.807808	4.217872e-50	2.371998e-47
MsG0380013988.01	MsG0380014042.01	0.813692	2.250130e-51	1.479630e-48
MsG0380013996.01	MsG0380014042.01	0.836604	8.489738e-57	1.075140e-53
MsG0380014007.01	MsG0380014042.01	0.805427	1.341706e-49	7.096404e-47
MsG0380014009.01	MsG0380014042.01	0.830668	2.579755e-55	2.736433e-52
MsG0380014010.01	MsG0380014042.01	0.946000	1.060440e-104	1.687144e-99
MsG0380014012.01	MsG0380014042.01	0.812003	5.275720e-51	3.315121e-48
MsG0380014013.01	MsG0380014042.01	0.817916	2.572746e-52	1.898325e-49
MsG0380014022.01	MsG0380014042.01	0.825863	3.716872e-54	3.429388e-51
MsG0380014037.01	MsG0380014042.01	0.853910	1.748726e-61	3.838161e-58
MsG0380014038.01	MsG0380014042.01	0.814558	1.448910e-51	9.753969e-49
MsG0380014041.01	MsG0380014042.01	0.826880	2.127846e-54	2.020976e-51
MsG0380014042.01	MsG0380014055.01	0.833409	5.421457e-56	6.238235e-53
MsG0380014042.01	MsG0380014056.01	0.963532	3.356886e-122	1.834488e-116
MsG0380014042.01	MsG0480020049.01	0.814941	1.192241e-51	8.108837e-49
MsG0380014042.01	MsG0580026430.01	0.814547	1.457144e-51	9.806338e-49
MsG0380014042.01	MsG0680033966.01	0.804934	1.701738e-49	8.887885e-47
MsG0380014042.01	MsG0780037705.01	0.826837	2.179335e-54	2.067236e-51
MsG0380014042.01	MsG0780040314.01	0.814800	1.281284e-51	8.681654e-49
MsG0380014042.01	MsG0880043541.01	0.816299	5.941306e-52	4.194156e-49
MsG0280007041.01	MsG0380014042.01	0.818038	2.415048e-52	1.787845e-49
MsG0280007042.01	MsG0380014042.01	0.803030	4.233927e-49	2.105467e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014042.01.T03	MTR_4g051420	96.296	27	1	0	1	27	66	92	1.46e-13	57.8
MsG0380014042.01.T03	MTR_3g035720	96.296	27	1	0	1	27	66	92	1.46e-13	57.8
MsG0380014042.01.T03	MTR_8g038480	92.593	27	2	0	1	27	54	80	6.45e-13	55.8
MsG0380014042.01.T02	MTR_4g051420	97.959	49	1	0	1	49	44	92	4.59e-31	103
MsG0380014042.01.T02	MTR_3g035720	97.959	49	1	0	1	49	44	92	4.59e-31	103
MsG0380014042.01.T02	MTR_8g038480	89.474	38	4	0	12	49	43	80	6.96e-21	77.0
MsG0380014042.01.T02	MTR_0002s0800	68.627	51	14	1	1	49	166	216	3.29e-18	73.9
MsG0380014042.01.T02	MTR_0002s0360	68.627	51	14	1	1	49	166	216	3.29e-18	73.9
MsG0380014042.01.T01	MTR_4g051420	98.913	92	1	0	1	92	1	92	3.93e-63	186
MsG0380014042.01.T01	MTR_3g035720	98.913	92	1	0	1	92	1	92	3.93e-63	186
MsG0380014042.01.T01	MTR_0002s0800	62.245	98	30	3	2	92	119	216	5.06e-34	116
MsG0380014042.01.T01	MTR_0002s0360	62.245	98	30	3	2	92	119	216	5.06e-34	116
MsG0380014042.01.T01	MTR_8g038480	89.474	38	4	0	55	92	43	80	2.08e-20	77.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380014042.01.T03	ATCG00820	92.593	27	2	0	1	27	66	92	1.46e-13	58.2
MsG0380014042.01.T02	ATCG00820	87.755	49	6	0	1	49	44	92	2.64e-28	96.3
MsG0380014042.01.T01	ATCG00820	85.870	92	13	0	1	92	1	92	3.38e-55	166
MsG0380014042.01.T01	AT5G47320	44.186	86	40	2	1	85	132	210	8.66e-14	63.9

Find 18 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTGAAAATTCTCCCAATTTA+TGG	0.137320	3:+48720539	None:intergenic
ATACGATCTGTTATATAAAT+AGG	0.289134	3:+48720568	None:intergenic
TCGTATGGTAGGCCATAAAT+TGG	0.313687	3:-48720551	MsG0380014042.01.T01:CDS
AGGTATAATGGTAGATGTTC+TGG	0.324700	3:+48720639	None:intergenic
CCCAATCATTGTAGGTATAA+TGG	0.326942	3:+48720627	None:intergenic
TTTCACCTACTCTAAACTTC+CGG	0.337945	3:-48720522	MsG0380014042.01.T01:CDS
ACCATTATACCTACAATGAT+TGG	0.350564	3:-48720628	MsG0380014042.01.T01:CDS
CATACCATTGCTATTCATAA+TGG	0.354095	3:-48720604	MsG0380014042.01.T01:CDS
TTCACCTACTCTAAACTTCC+GGG	0.355100	3:-48720521	MsG0380014042.01.T01:CDS
AATAAATGATTCGCTACAAA+AGG	0.410516	3:+48720718	None:intergenic
ATGGTATGCCCAATCATTGT+AGG	0.463349	3:+48720619	None:intergenic
ATAAATAAGCTTAACACAAA+AGG	0.463559	3:-48720688	MsG0380014042.01.T01:CDS
CCATTATACCTACAATGATT+GGG	0.487902	3:-48720627	MsG0380014042.01.T01:CDS
CGTATGGTAGGCCATAAATT+GGG	0.512944	3:-48720550	MsG0380014042.01.T01:CDS
TATATAACAGATCGTATGGT+AGG	0.535301	3:-48720562	MsG0380014042.01.T01:CDS
TATTTATATAACAGATCGTA+TGG	0.571298	3:-48720566	MsG0380014042.01.T01:CDS
CACCTACTCTAAACTTCCGG+GGG	0.635563	3:-48720519	MsG0380014042.01.T01:CDS
TCACCTACTCTAAACTTCCG+GGG	0.640846	3:-48720520	MsG0380014042.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAAAGAAATAATAATAACT+TGG	-	Chr3:48720565-48720584	MsG0380014042.01.T01:CDS	10.0%
!!	ATAAATAAGCTTAACACAAA+AGG	-	Chr3:48720540-48720559	MsG0380014042.01.T01:CDS	20.0%
!!	ATACGATCTGTTATATAAAT+AGG	+	Chr3:48720663-48720682	None:intergenic	20.0%
!!	TATTTATATAACAGATCGTA+TGG	-	Chr3:48720662-48720681	MsG0380014042.01.T01:CDS	20.0%
!	AATAAATGATTCGCTACAAA+AGG	+	Chr3:48720513-48720532	None:intergenic	25.0%
!!	TTTTCCATTATGAATAGCAA+TGG	+	Chr3:48720631-48720650	None:intergenic	25.0%
	ACCATTATACCTACAATGAT+TGG	-	Chr3:48720600-48720619	MsG0380014042.01.T01:CDS	30.0%
	CCATTATACCTACAATGATT+GGG	-	Chr3:48720601-48720620	MsG0380014042.01.T01:CDS	30.0%
	GTGAAAATTCTCCCAATTTA+TGG	+	Chr3:48720692-48720711	None:intergenic	30.0%
	TATATAACAGATCGTATGGT+AGG	-	Chr3:48720666-48720685	MsG0380014042.01.T01:CDS	30.0%
!	CATACCATTGCTATTCATAA+TGG	-	Chr3:48720624-48720643	MsG0380014042.01.T01:CDS	30.0%
	AGGTATAATGGTAGATGTTC+TGG	+	Chr3:48720592-48720611	None:intergenic	35.0%
	CCCAATCATTGTAGGTATAA+TGG	+	Chr3:48720604-48720623	None:intergenic	35.0%
	TTTCACCTACTCTAAACTTC+CGG	-	Chr3:48720706-48720725	MsG0380014042.01.T01:CDS	35.0%
	ATGGTATGCCCAATCATTGT+AGG	+	Chr3:48720612-48720631	None:intergenic	40.0%
	CGTATGGTAGGCCATAAATT+GGG	-	Chr3:48720678-48720697	MsG0380014042.01.T01:CDS	40.0%
	TCGTATGGTAGGCCATAAAT+TGG	-	Chr3:48720677-48720696	MsG0380014042.01.T01:CDS	40.0%
	TTCACCTACTCTAAACTTCC+GGG	-	Chr3:48720707-48720726	MsG0380014042.01.T01:CDS	40.0%
!!	TATCATTTTTCGCATACCCC+CGG	+	Chr3:48720728-48720747	None:intergenic	40.0%
	TCACCTACTCTAAACTTCCG+GGG	-	Chr3:48720708-48720727	MsG0380014042.01.T01:CDS	45.0%
	CACCTACTCTAAACTTCCGG+GGG	-	Chr3:48720709-48720728	MsG0380014042.01.T01:CDS	50.0%
!	TACCCCCGGAAGTTTAGAGT+AGG	+	Chr3:48720714-48720733	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	48720486	48720764	48720486	ID=MsG0380014042.01;Name=MsG0380014042.01
Chr3	mRNA	48720486	48720764	48720486	ID=MsG0380014042.01.T01;Parent=MsG0380014042.01;Name=MsG0380014042.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|92
Chr3	exon	48720486	48720764	48720486	ID=MsG0380014042.01.T01:exon:9392;Parent=MsG0380014042.01.T01
Chr3	CDS	48720486	48720764	48720486	ID=MsG0380014042.01.T01:cds;Parent=MsG0380014042.01.T01
Chr3	mRNA	48720486	48720635	48720486	ID=MsG0380014042.01.T02;Parent=MsG0380014042.01;Name=MsG0380014042.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|49
Chr3	exon	48720486	48720635	48720486	ID=MsG0380014042.01.T02:exon:9393;Parent=MsG0380014042.01.T02
Chr3	CDS	48720486	48720635	48720486	ID=MsG0380014042.01.T02:cds;Parent=MsG0380014042.01.T02
Chr3	mRNA	48720486	48720569	48720486	ID=MsG0380014042.01.T03;Parent=MsG0380014042.01;Name=MsG0380014042.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|27
Chr3	exon	48720486	48720569	48720486	ID=MsG0380014042.01.T03:exon:9394;Parent=MsG0380014042.01.T03
Chr3	CDS	48720486	48720569	48720486	ID=MsG0380014042.01.T03:cds;Parent=MsG0380014042.01.T03

Gene Sequence

>MsG0380014042.01.T03

ATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

>MsG0380014042.01.T02

ATGATTGGGCATACCATTGCTATTCATAATGGAAAAGAGCATTTACCTATTTATATAACAGATCGTATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

>MsG0380014042.01.T01

ATGACACGTTCACGAAAAAAAAATCCTTTTGTAGCGAATCATTTATTAAAAAAAATAAATAAGCTTAACACAAAAGGAGAAAAAGAAATAATAATAACTTGGTCCAGAACATCTACCATTATACCTACAATGATTGGGCATACCATTGCTATTCATAATGGAAAAGAGCATTTACCTATTTATATAACAGATCGTATGGTAGGCCATAAATTGGGAGAATTTTCACCTACTCTAAACTTCCGGGGGTATGCGAAAAATGATAATAGATCGCGTCGTTAA

Protein sequence

>MsG0380014042.01.T03

MVGHKLGEFSPTLNFRGYAKNDNRSRR*

>MsG0380014042.01.T02

MIGHTIAIHNGKEHLPIYITDRMVGHKLGEFSPTLNFRGYAKNDNRSRR*

>MsG0380014042.01.T01

MTRSRKKNPFVANHLLKKINKLNTKGEKEIIITWSRTSTIIPTMIGHTIAIHNGKEHLPIYITDRMVGHKLGEFSPTLNFRGYAKNDNRSRR*