AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380018029.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018029.01.T01 MTR_3g117500 100.000 85 0 0 1 85 396 480 3.98e-57 182
MsG0380018029.01.T01 MTR_4g035975 67.059 85 28 0 1 85 401 485 9.61e-36 126
MsG0380018029.01.T01 MTR_7g108925 63.095 84 31 0 1 84 110 193 1.43e-34 117
MsG0380018029.01.T01 MTR_4g035930 63.529 85 31 0 1 85 379 463 2.67e-34 122
MsG0380018029.01.T01 MTR_3g080810 65.882 85 29 0 1 85 405 489 4.06e-34 121
MsG0380018029.01.T01 MTR_4g035955 62.353 85 32 0 1 85 378 462 1.50e-33 120
MsG0380018029.01.T01 MTR_8g104820 66.279 86 28 1 1 85 401 486 1.06e-32 117
MsG0380018029.01.T01 MTR_5g010000 62.353 85 31 1 1 84 403 487 1.08e-32 117
MsG0380018029.01.T01 MTR_5g009970 60.000 85 33 1 1 84 303 387 1.34e-31 114
MsG0380018029.01.T01 MTR_5g009970 60.000 85 33 1 1 84 404 488 1.36e-31 115
MsG0380018029.01.T01 MTR_1g088190 61.176 85 33 0 1 85 390 474 1.67e-31 114
MsG0380018029.01.T01 MTR_5g009970 60.000 85 33 1 1 84 235 319 2.07e-31 112
MsG0380018029.01.T01 MTR_5g009970 60.000 85 33 1 1 84 330 414 1.12e-30 111
MsG0380018029.01.T01 MTR_3g010330 62.353 85 31 1 1 84 391 475 3.99e-29 108
MsG0380018029.01.T01 MTR_8g099410 55.814 86 37 1 1 85 402 487 6.51e-28 105
MsG0380018029.01.T01 MTR_8g099410 55.814 86 37 1 1 85 402 487 6.73e-28 105
MsG0380018029.01.T01 MTR_0393s0050 59.740 77 30 1 1 76 87 163 4.46e-26 95.1
MsG0380018029.01.T01 MTR_1g037340 54.651 86 37 2 1 85 393 477 2.55e-24 94.7
MsG0380018029.01.T01 MTR_1g037320 51.163 86 40 2 1 85 203 287 8.57e-23 88.6
MsG0380018029.01.T01 MTR_5g093590 46.512 86 45 1 1 85 411 496 2.90e-22 89.0
MsG0380018029.01.T01 MTR_3g080190 46.512 86 45 1 1 85 416 501 4.51e-22 88.6
MsG0380018029.01.T01 MTR_5g085730 45.349 86 46 1 1 85 407 492 1.01e-21 87.4
MsG0380018029.01.T01 MTR_8g075990 49.412 85 39 1 1 85 440 520 1.18e-21 87.4
MsG0380018029.01.T01 MTR_4g074960 45.349 86 46 1 1 85 403 488 1.26e-21 87.4
MsG0380018029.01.T01 MTR_3g113720 44.706 85 43 1 1 85 434 514 2.30e-21 86.7
MsG0380018029.01.T01 MTR_3g090540 44.578 83 43 1 1 83 498 577 5.84e-19 79.7
MsG0380018029.01.T01 MTR_3g023740 44.186 86 46 2 1 85 391 475 3.20e-17 74.7
MsG0380018029.01.T01 MTR_2g028480 43.529 85 45 1 1 85 504 585 9.09e-17 73.6
MsG0380018029.01.T01 MTR_3g105230 45.000 80 42 2 1 79 391 469 2.42e-16 72.4
MsG0380018029.01.T01 MTR_4g128810 42.353 85 46 1 1 85 248 329 3.39e-15 68.9
MsG0380018029.01.T01 MTR_3g082440 41.176 85 47 2 1 85 506 587 2.72e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380018029.01.T01 AT4G39010 81.176 85 16 0 1 85 402 486 2.87e-39 135
MsG0380018029.01.T01 AT1G70710 64.706 85 30 0 1 85 397 481 3.61e-34 122
MsG0380018029.01.T01 AT1G23210 61.176 85 33 0 1 85 397 481 5.27e-33 118
MsG0380018029.01.T01 AT1G02800 63.095 84 31 0 1 84 410 493 6.57e-33 118
MsG0380018029.01.T01 AT4G02290 59.524 84 34 0 1 84 421 504 8.74e-33 118
MsG0380018029.01.T01 AT4G02290 58.824 85 35 0 1 85 309 393 6.98e-32 114
MsG0380018029.01.T01 AT4G23560 59.524 84 34 0 1 84 386 469 1.81e-31 114
MsG0380018029.01.T01 AT4G38990 60.000 85 34 0 1 85 401 485 4.72e-31 113
MsG0380018029.01.T01 AT4G09740 59.524 84 34 0 1 84 386 469 1.01e-30 112
MsG0380018029.01.T01 AT4G39000 56.471 85 37 0 1 85 398 482 4.33e-29 108
MsG0380018029.01.T01 AT1G22880 58.333 84 35 0 1 84 306 389 7.13e-29 106
MsG0380018029.01.T01 AT1G22880 58.333 84 35 0 1 84 390 473 1.46e-28 106
MsG0380018029.01.T01 AT1G71380 57.143 84 36 0 1 84 390 473 2.35e-27 103
MsG0380018029.01.T01 AT2G44570 54.651 86 38 1 1 85 402 487 3.02e-27 103
MsG0380018029.01.T01 AT2G44540 50.000 86 42 1 1 85 401 486 1.52e-24 95.5
MsG0380018029.01.T01 AT1G64390 51.163 86 41 1 1 85 399 484 1.65e-24 95.5
MsG0380018029.01.T01 AT2G44550 47.674 86 44 1 1 85 400 485 9.86e-24 93.2
MsG0380018029.01.T01 AT2G32990 48.837 86 43 1 1 85 415 500 3.76e-23 91.7
MsG0380018029.01.T01 AT4G11050 47.674 86 44 1 1 85 400 485 2.15e-22 89.7
MsG0380018029.01.T01 AT4G11050 47.674 86 44 1 1 85 400 485 2.18e-22 89.7
MsG0380018029.01.T01 AT4G11050 47.674 86 44 1 1 85 400 485 2.72e-22 89.4
MsG0380018029.01.T01 AT1G48930 48.837 86 43 1 1 85 404 489 3.86e-22 89.0
MsG0380018029.01.T01 AT2G44560 52.326 86 40 1 1 85 401 486 9.77e-22 87.8
MsG0380018029.01.T01 AT1G75680 47.500 80 38 1 1 80 430 505 5.47e-21 85.5
MsG0380018029.01.T01 AT1G19940 45.238 84 42 1 1 84 421 500 3.45e-20 83.2
MsG0380018029.01.T01 AT1G19940 45.238 84 42 1 1 84 421 500 3.45e-20 83.2
MsG0380018029.01.T01 AT1G65610 44.048 84 44 1 1 84 505 585 1.29e-18 79.0
MsG0380018029.01.T01 AT5G49720 42.353 85 46 1 1 85 501 582 1.58e-15 70.1
MsG0380018029.01.T01 AT3G43860 41.860 86 48 2 1 85 395 479 1.56e-14 67.4
MsG0380018029.01.T01 AT3G43860 41.860 86 48 2 1 85 395 479 1.56e-14 67.4
MsG0380018029.01.T01 AT4G24260 38.824 85 48 1 1 85 502 582 4.63e-13 63.2

Find 28 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATCTCTACCTTCTATTA+AGG 0.295970 3:+103520303 MsG0380018029.01.T01:CDS
TATATTAATGCGCCATTTGT+TGG 0.343277 3:+103520484 MsG0380018029.01.T01:CDS
TTATCTTACATGGTTGGTTA+TGG 0.346424 3:+103520247 None:intergenic
ATATTAATGCGCCATTTGTT+GGG 0.347405 3:+103520485 MsG0380018029.01.T01:CDS
CCTAACCCAAATGTTCTTGT+AGG 0.353533 3:+103520382 MsG0380018029.01.T01:CDS
ATAGCTCCTACAAGAACATT+TGG 0.366293 3:-103520388 None:intergenic
CCTACAAGAACATTTGGGTT+AGG 0.401102 3:-103520382 None:intergenic
CAACAGATTGCTTGCAAGGA+AGG 0.449168 3:+103520334 MsG0380018029.01.T01:CDS
GGAGCTATTGTGGGAGGACC+TGG 0.473308 3:+103520403 MsG0380018029.01.T01:CDS
TAGCTCCTACAAGAACATTT+GGG 0.479600 3:-103520387 None:intergenic
TGAGGATGGTCCTTAATAGA+AGG 0.486620 3:-103520313 None:intergenic
TTGGGTTTATCTTACATGGT+TGG 0.498954 3:+103520241 None:intergenic
TGTTCTTGTAGGAGCTATTG+TGG 0.507021 3:+103520393 MsG0380018029.01.T01:CDS
ATATGCTAAACCCCAACAAA+TGG 0.512665 3:-103520496 None:intergenic
ATGATGTTTATGTGGATGAC+AGG 0.521412 3:+103520431 MsG0380018029.01.T01:CDS
TCCTCAACAGATTGCTTGCA+AGG 0.531094 3:+103520330 MsG0380018029.01.T01:CDS
AAATGGCGCATTAATATATG+TGG 0.534377 3:-103520479 None:intergenic
GTTCTTGTAGGAGCTATTGT+GGG 0.555313 3:+103520394 MsG0380018029.01.T01:CDS
TCCTTGCAAGCAATCTGTTG+AGG 0.555459 3:-103520331 None:intergenic
ACATAAACATCATCTTCTCC+AGG 0.567710 3:-103520421 None:intergenic
TATTAATGCGCCATTTGTTG+GGG 0.570106 3:+103520486 MsG0380018029.01.T01:CDS
GGCGCATTAATATATGTGGT+TGG 0.580585 3:-103520475 None:intergenic
CTTGTAGGAGCTATTGTGGG+AGG 0.586975 3:+103520397 MsG0380018029.01.T01:CDS
TGGAGAAGATGATGTTTATG+TGG 0.587154 3:+103520423 MsG0380018029.01.T01:CDS
TGCAAGCAATCTGTTGAGGA+TGG 0.591228 3:-103520327 None:intergenic
CCACGATGATGAATACGTTG+TGG 0.695422 3:-103520280 None:intergenic
CCACAACGTATTCATCATCG+TGG 0.724350 3:+103520280 MsG0380018029.01.T01:CDS
TGATGTTTATGTGGATGACA+GGG 0.726322 3:+103520432 MsG0380018029.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATGGCGCATTAATATATG+TGG - Chr3:103520482-103520501 None:intergenic 30.0%
ATATTAATGCGCCATTTGTT+GGG + Chr3:103520485-103520504 MsG0380018029.01.T01:CDS 30.0%
TATATTAATGCGCCATTTGT+TGG + Chr3:103520484-103520503 MsG0380018029.01.T01:CDS 30.0%
ACATAAACATCATCTTCTCC+AGG - Chr3:103520424-103520443 None:intergenic 35.0%
ATAGCTCCTACAAGAACATT+TGG - Chr3:103520391-103520410 None:intergenic 35.0%
ATGATGTTTATGTGGATGAC+AGG + Chr3:103520431-103520450 MsG0380018029.01.T01:CDS 35.0%
CTCATCTCTACCTTCTATTA+AGG + Chr3:103520303-103520322 MsG0380018029.01.T01:CDS 35.0%
TAGCTCCTACAAGAACATTT+GGG - Chr3:103520390-103520409 None:intergenic 35.0%
TGATGTTTATGTGGATGACA+GGG + Chr3:103520432-103520451 MsG0380018029.01.T01:CDS 35.0%
TGGAGAAGATGATGTTTATG+TGG + Chr3:103520423-103520442 MsG0380018029.01.T01:CDS 35.0%
! TATTAATGCGCCATTTGTTG+GGG + Chr3:103520486-103520505 MsG0380018029.01.T01:CDS 35.0%
CCTAACCCAAATGTTCTTGT+AGG + Chr3:103520382-103520401 MsG0380018029.01.T01:CDS 40.0%
GGCGCATTAATATATGTGGT+TGG - Chr3:103520478-103520497 None:intergenic 40.0%
GTTCTTGTAGGAGCTATTGT+GGG + Chr3:103520394-103520413 MsG0380018029.01.T01:CDS 40.0%
TGAGGATGGTCCTTAATAGA+AGG - Chr3:103520316-103520335 None:intergenic 40.0%
TGTTCTTGTAGGAGCTATTG+TGG + Chr3:103520393-103520412 MsG0380018029.01.T01:CDS 40.0%
! CCTACAAGAACATTTGGGTT+AGG - Chr3:103520385-103520404 None:intergenic 40.0%
CAACAGATTGCTTGCAAGGA+AGG + Chr3:103520334-103520353 MsG0380018029.01.T01:CDS 45.0%
CCACAACGTATTCATCATCG+TGG + Chr3:103520280-103520299 MsG0380018029.01.T01:CDS 45.0%
CCACGATGATGAATACGTTG+TGG - Chr3:103520283-103520302 None:intergenic 45.0%
TCCTCAACAGATTGCTTGCA+AGG + Chr3:103520330-103520349 MsG0380018029.01.T01:CDS 45.0%
TCCTTGCAAGCAATCTGTTG+AGG - Chr3:103520334-103520353 None:intergenic 45.0%
TGCAAGCAATCTGTTGAGGA+TGG - Chr3:103520330-103520349 None:intergenic 45.0%
CTTGTAGGAGCTATTGTGGG+AGG + Chr3:103520397-103520416 MsG0380018029.01.T01:CDS 50.0%
GGAGCTATTGTGGGAGGACC+TGG + Chr3:103520403-103520422 MsG0380018029.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 103520256 103520513 103520256 ID=MsG0380018029.01;Name=MsG0380018029.01
Chr3 mRNA 103520256 103520513 103520256 ID=MsG0380018029.01.T01;Parent=MsG0380018029.01;Name=MsG0380018029.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|85
Chr3 exon 103520256 103520513 103520256 ID=MsG0380018029.01.T01:exon:13474;Parent=MsG0380018029.01.T01
Chr3 CDS 103520256 103520513 103520256 ID=MsG0380018029.01.T01:cds;Parent=MsG0380018029.01.T01
Gene Sequence

>MsG0380018029.01.T01

ATGGTTGGTTATGGTAACAATTATCCACAACGTATTCATCATCGTGGCTCATCTCTACCTTCTATTAAGGACCATCCTCAACAGATTGCTTGCAAGGAAGGTTCAATATACTTCAATTCAACAAATCCTAACCCAAATGTTCTTGTAGGAGCTATTGTGGGAGGACCTGGAGAAGATGATGTTTATGTGGATGACAGGGCTGATTATAGAAAATCTGAGCCAACCACATATATTAATGCGCCATTTGTTGGGGTTTAG

Protein sequence

>MsG0380018029.01.T01

MVGYGNNYPQRIHHRGSSLPSIKDHPQQIACKEGSIYFNSTNPNPNVLVGAIVGGPGEDDVYVDDRADYRKSEPTTYINAPFVGV*