Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019020.01.T01 | PNX71200.1 | 93.421 | 76 | 5 | 0 | 1 | 76 | 457 | 532 | 8.95E-39 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019020.01.T01 | Q9FKB6 | 82.192 | 73 | 13 | 0 | 4 | 76 | 507 | 579 | 3.51E-34 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019020.01.T01 | A0A2K3KY37 | 93.421 | 76 | 5 | 0 | 1 | 76 | 457 | 532 | 4.28e-39 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000178.01 | MsG0480019020.01 | 0.837590 | 4.752967e-57 | 6.203058e-54 |
| MsG0180003669.01 | MsG0480019020.01 | 0.816255 | 6.075291e-52 | 4.283641e-49 |
| MsG0180003920.01 | MsG0480019020.01 | 0.809571 | 1.772146e-50 | 1.044197e-47 |
| MsG0280011014.01 | MsG0480019020.01 | 0.802873 | 4.563090e-49 | 2.260151e-46 |
| MsG0280011282.01 | MsG0480019020.01 | 0.815104 | 1.096732e-51 | 7.492032e-49 |
| MsG0380012471.01 | MsG0480019020.01 | 0.831898 | 1.285730e-55 | 1.414593e-52 |
| MsG0380013475.01 | MsG0480019020.01 | 0.838243 | 3.228734e-57 | 4.298414e-54 |
| MsG0480019020.01 | MsG0480023832.01 | 0.804793 | 1.821799e-49 | 9.479951e-47 |
| MsG0480019020.01 | MsG0480024003.01 | 0.800582 | 1.347234e-48 | 6.296098e-46 |
| MsG0480019020.01 | MsG0580028990.01 | 0.805501 | 1.295080e-49 | 6.862559e-47 |
| MsG0480019020.01 | MsG0680035475.01 | 0.803927 | 2.759324e-49 | 1.404264e-46 |
| MsG0480019020.01 | MsG0780036477.01 | 0.803039 | 4.215739e-49 | 2.096892e-46 |
| MsG0480019020.01 | MsG0780038386.01 | 0.841624 | 4.243755e-58 | 6.272113e-55 |
| MsG0480019020.01 | MsG0780038424.01 | 0.818474 | 1.923708e-52 | 1.441466e-49 |
| MsG0480019020.01 | MsG0780039137.01 | 0.810486 | 1.125555e-50 | 6.793264e-48 |
| MsG0480019020.01 | MsG0880044080.01 | 0.818779 | 1.640403e-52 | 1.239518e-49 |
| MsG0480019020.01 | MsG0880044081.01 | 0.805383 | 1.370541e-49 | 7.240585e-47 |
| MsG0480019020.01 | MsG0880045691.01 | 0.822085 | 2.859817e-53 | 2.369607e-50 |
| MsG0480019020.01 | MsG0880046012.01 | 0.829682 | 4.490021e-55 | 4.628612e-52 |
| MsG0380015566.01 | MsG0480019020.01 | 0.811451 | 6.954675e-51 | 4.306533e-48 |
| MsG0380015677.01 | MsG0480019020.01 | 0.821476 | 3.957263e-53 | 3.222830e-50 |
| MsG0280006712.01 | MsG0480019020.01 | 0.802596 | 5.204361e-49 | 2.559674e-46 |
| MsG0280009514.01 | MsG0480019020.01 | 0.804319 | 2.287141e-49 | 1.175637e-46 |
| MsG0280009868.01 | MsG0480019020.01 | 0.815125 | 1.084979e-51 | 7.416199e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019020.01.T01 | MTR_4g022330 | 93.421 | 76 | 5 | 0 | 1 | 76 | 507 | 582 | 1.56e-42 | 146 |
| MsG0480019020.01.T01 | MTR_7g108290 | 62.319 | 69 | 25 | 1 | 6 | 73 | 522 | 590 | 4.84e-22 | 89.4 |
| MsG0480019020.01.T01 | MTR_1g088885 | 61.333 | 75 | 28 | 1 | 6 | 79 | 519 | 593 | 6.55e-22 | 89.0 |
| MsG0480019020.01.T01 | MTR_7g073980 | 58.904 | 73 | 26 | 2 | 4 | 73 | 504 | 575 | 3.88e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019020.01.T01 | AT5G48800 | 82.192 | 73 | 13 | 0 | 4 | 76 | 507 | 579 | 3.58e-35 | 126 |
| MsG0480019020.01.T01 | AT1G03010 | 56.098 | 82 | 35 | 1 | 6 | 86 | 529 | 610 | 7.44e-22 | 89.0 |
| MsG0480019020.01.T01 | AT1G03010 | 56.098 | 82 | 35 | 1 | 6 | 86 | 502 | 583 | 7.61e-22 | 89.0 |
| MsG0480019020.01.T01 | AT1G03010 | 56.098 | 82 | 35 | 1 | 6 | 86 | 528 | 609 | 9.40e-22 | 88.6 |
| MsG0480019020.01.T01 | AT2G47860 | 66.102 | 59 | 20 | 0 | 6 | 64 | 519 | 577 | 4.64e-20 | 84.0 |
| MsG0480019020.01.T01 | AT2G47860 | 66.102 | 59 | 20 | 0 | 6 | 64 | 542 | 600 | 4.79e-20 | 83.6 |
| MsG0480019020.01.T01 | AT2G47860 | 66.102 | 59 | 20 | 0 | 6 | 64 | 401 | 459 | 5.20e-20 | 83.6 |
| MsG0480019020.01.T01 | AT2G47860 | 66.102 | 59 | 20 | 0 | 6 | 64 | 401 | 459 | 5.20e-20 | 83.6 |
| MsG0480019020.01.T01 | AT3G08660 | 47.500 | 80 | 39 | 2 | 3 | 79 | 478 | 557 | 3.82e-17 | 75.5 |
| MsG0480019020.01.T01 | AT3G08660 | 47.500 | 80 | 39 | 2 | 3 | 79 | 418 | 497 | 4.23e-17 | 75.5 |
| MsG0480019020.01.T01 | AT3G08570 | 44.318 | 88 | 48 | 1 | 3 | 89 | 506 | 593 | 1.40e-14 | 68.2 |
Find 21 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAGTAAACTTAGTCTCTT+TGG | 0.222466 | 4:-13392283 | MsG0480019020.01.T01:CDS |
| ACTTTGCCATATCCCTCTTC+AGG | 0.383164 | 4:+13392343 | None:intergenic |
| GAGAAACAAGTTCTCCATCT+AGG | 0.434372 | 4:-13392243 | MsG0480019020.01.T01:CDS |
| AAGAGGGATATGGCAAAGTC+TGG | 0.448980 | 4:-13392339 | MsG0480019020.01.T01:CDS |
| GTTCTATGAGAATTCCTAGA+TGG | 0.450370 | 4:+13392229 | None:intergenic |
| ATTTAGTCTCATTCGTAGTC+GGG | 0.454403 | 4:+13392389 | None:intergenic |
| GGGAACATGTTTGCCTGAAG+AGG | 0.480581 | 4:-13392356 | MsG0480019020.01.T01:CDS |
| CATTTAGTCTCATTCGTAGT+CGG | 0.490854 | 4:+13392388 | None:intergenic |
| TGTTTGCCTGAAGAGGGATA+TGG | 0.493690 | 4:-13392349 | MsG0480019020.01.T01:CDS |
| ATGCGCCATGACGCATGCAT+TGG | 0.506952 | 4:+13392501 | None:intergenic |
| TTCTCATAGAACAGATTCAA+AGG | 0.568182 | 4:-13392218 | MsG0480019020.01.T01:CDS |
| TGGAGAACTTGTTTCTCTAG+AGG | 0.571879 | 4:+13392249 | None:intergenic |
| GCGTCATGGCGCATAAGTAG+CGG | 0.572194 | 4:-13392492 | MsG0480019020.01.T01:CDS |
| GGAACATGTTTGCCTGAAGA+GGG | 0.578661 | 4:-13392355 | MsG0480019020.01.T01:CDS |
| TCAAGCCAATGCATGCGTCA+TGG | 0.590453 | 4:-13392506 | None:intergenic |
| TGAGACTAAATGATCTAGAG+AGG | 0.591007 | 4:-13392377 | MsG0480019020.01.T01:CDS |
| GTGATAACTACGCATCATTG+AGG | 0.598172 | 4:-13392443 | MsG0480019020.01.T01:CDS |
| CCGTGAGCTAAAACTCGAGT+TGG | 0.614100 | 4:-13392412 | MsG0480019020.01.T01:CDS |
| GAGACTAAATGATCTAGAGA+GGG | 0.643240 | 4:-13392376 | MsG0480019020.01.T01:CDS |
| AAGTAGCGGTGCACTAAGTG+CGG | 0.644572 | 4:-13392478 | MsG0480019020.01.T01:CDS |
| AATGATGCGTAGTTATCACG+TGG | 0.694936 | 4:+13392447 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTAGTAAACTTAGTCTCTT+TGG | - | Chr4:13392430-13392449 | MsG0480019020.01.T01:CDS | 25.0% |
| TTCTCATAGAACAGATTCAA+AGG | - | Chr4:13392495-13392514 | MsG0480019020.01.T01:CDS | 30.0% | |
| ATTTAGTCTCATTCGTAGTC+GGG | + | Chr4:13392327-13392346 | None:intergenic | 35.0% | |
| CATTTAGTCTCATTCGTAGT+CGG | + | Chr4:13392328-13392347 | None:intergenic | 35.0% | |
| GAGACTAAATGATCTAGAGA+GGG | - | Chr4:13392337-13392356 | MsG0480019020.01.T01:CDS | 35.0% | |
| GTTCTATGAGAATTCCTAGA+TGG | + | Chr4:13392487-13392506 | None:intergenic | 35.0% | |
| TGAGACTAAATGATCTAGAG+AGG | - | Chr4:13392336-13392355 | MsG0480019020.01.T01:CDS | 35.0% | |
| AATGATGCGTAGTTATCACG+TGG | + | Chr4:13392269-13392288 | None:intergenic | 40.0% | |
| GAGAAACAAGTTCTCCATCT+AGG | - | Chr4:13392470-13392489 | MsG0480019020.01.T01:CDS | 40.0% | |
| GTGATAACTACGCATCATTG+AGG | - | Chr4:13392270-13392289 | MsG0480019020.01.T01:CDS | 40.0% | |
| ! | TGGAGAACTTGTTTCTCTAG+AGG | + | Chr4:13392467-13392486 | None:intergenic | 40.0% |
| AAGAGGGATATGGCAAAGTC+TGG | - | Chr4:13392374-13392393 | MsG0480019020.01.T01:CDS | 45.0% | |
| ACTTTGCCATATCCCTCTTC+AGG | + | Chr4:13392373-13392392 | None:intergenic | 45.0% | |
| GGAACATGTTTGCCTGAAGA+GGG | - | Chr4:13392358-13392377 | MsG0480019020.01.T01:CDS | 45.0% | |
| TGTTTGCCTGAAGAGGGATA+TGG | - | Chr4:13392364-13392383 | MsG0480019020.01.T01:CDS | 45.0% | |
| !!! | CCAACTCGAGTTTTAGCTCA+CGG | + | Chr4:13392304-13392323 | None:intergenic | 45.0% |
| AAGTAGCGGTGCACTAAGTG+CGG | - | Chr4:13392235-13392254 | MsG0480019020.01.T01:CDS | 50.0% | |
| CCGTGAGCTAAAACTCGAGT+TGG | - | Chr4:13392301-13392320 | MsG0480019020.01.T01:CDS | 50.0% | |
| GGGAACATGTTTGCCTGAAG+AGG | - | Chr4:13392357-13392376 | MsG0480019020.01.T01:CDS | 50.0% | |
| GCGTCATGGCGCATAAGTAG+CGG | - | Chr4:13392221-13392240 | MsG0480019020.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 13392215 | 13392520 | 13392215 | ID=MsG0480019020.01;Name=MsG0480019020.01 |
| Chr4 | mRNA | 13392215 | 13392520 | 13392215 | ID=MsG0480019020.01.T01;Parent=MsG0480019020.01;Name=MsG0480019020.01.T01;_AED=0.45;_eAED=0.52;_QI=0|-1|0|1|-1|1|1|0|101 |
| Chr4 | exon | 13392215 | 13392520 | 13392215 | ID=MsG0480019020.01.T01:exon:8388;Parent=MsG0480019020.01.T01 |
| Chr4 | CDS | 13392215 | 13392520 | 13392215 | ID=MsG0480019020.01.T01:cds;Parent=MsG0480019020.01.T01 |
| Gene Sequence |
| Protein sequence |
