AlfalfaGEDB | MsG0480020919.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020919.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	XP_003606627.1	99.324	148	1	0	1	148	1	148	3.31E-98	289

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	Q1S9I9	99.324	148	1	0	1	148	1	148	8.77E-101	288

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	G7JTQ7	99.324	148	1	0	1	148	1	148	1.58e-98	289

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480020919.01	MsG0480020920.01	0.832934	7.118598e-56	8.075213e-53
MsG0480020919.01	MsG0480020969.01	0.804792	1.822551e-49	9.483673e-47
MsG0480020919.01	MsG0480021015.01	0.809961	1.460951e-50	8.697249e-48
MsG0480020919.01	MsG0480021049.01	0.825553	4.403436e-54	4.026721e-51

PPI

Gene1	Gene2	Type
MsG0480020919.01	MsG0880045015.01	PPI
MsG0480020919.01	MsG0480023842.01	PPI
MsG0180000243.01	MsG0480020919.01	PPI
MsG0480020919.01	MsG0880047260.01	PPI
MsG0480020919.01	MsG0480022307.01	PPI
MsG0480020919.01	MsG0280008380.01	PPI
MsG0480020919.01	MsG0580025504.01	PPI
MsG0480020919.01	MsG0780036534.01	PPI
MsG0480020919.01	MsG0780041592.01	PPI
MsG0480020919.01	MsG0880042844.01	PPI
MsG0780038642.01	MsG0480020919.01	PPI
MsG0880044910.01	MsG0480020919.01	PPI
MsG0480020877.01	MsG0480020919.01	PPI
MsG0480020919.01	MsG0380012788.01	PPI
MsG0480020919.01	MsG0880044910.01	PPI
MsG0580029044.01	MsG0480020919.01	PPI
MsG0580027231.01	MsG0480020919.01	PPI
MsG0480020919.01	MsG0280007919.01	PPI
MsG0580025506.01	MsG0480020919.01	PPI
MsG0480020919.01	MsG0780041593.01	PPI
MsG0480020919.01	MsG0580029194.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	MTR_4g063220	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g063990	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g063200	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g064020	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g071180	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480020919.01.T01	MTR_4g070240	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480020919.01.T01	MTR_4g070245	97.973	148	3	0	1	148	1	148	4.20e-100	284
MsG0480020919.01.T01	MTR_4g063240	98.649	148	1	1	1	148	1	147	1.15e-99	283
MsG0480020919.01.T01	MTR_4g063275	96.622	148	5	0	1	148	1	148	8.49e-99	280
MsG0480020919.01.T01	MTR_4g064983	98.611	144	2	0	1	144	1	144	8.23e-97	279
MsG0480020919.01.T01	MTR_4g088025	93.919	148	4	1	1	148	1	143	8.30e-89	255
MsG0480020919.01.T01	MTR_1g090780	88.514	148	6	3	1	148	1	137	1.68e-78	229
MsG0480020919.01.T01	MTR_1g492910	87.838	148	7	3	1	148	1	137	8.34e-78	227
MsG0480020919.01.T01	MTR_1g068600	92.793	111	4	1	38	148	30	136	3.40e-64	192
MsG0480020919.01.T01	MTR_7g099960	92.793	111	5	1	38	148	31	138	6.27e-64	192
MsG0480020919.01.T01	MTR_2g084480	61.798	89	34	0	59	147	136	224	4.83e-37	127
MsG0480020919.01.T01	MTR_8g069990	68.817	93	21	3	15	107	4	88	3.55e-34	115
MsG0480020919.01.T01	MTR_4g063270	90.196	51	5	0	86	136	1	51	5.24e-27	95.9
MsG0480020919.01.T01	MTR_7g114580	90.244	41	4	0	108	148	11	51	9.46e-20	77.4
MsG0480020919.01.T01	MTR_4g032777	36.792	106	63	2	31	132	5	110	1.73e-13	63.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	1.47e-83	242
MsG0480020919.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	9.69e-81	235
MsG0480020919.01.T01	AT1G07790	86.928	153	10	3	1	148	1	148	7.69e-80	233
MsG0480020919.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.65e-77	226
MsG0480020919.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	8.66e-76	222
MsG0480020919.01.T01	AT2G37470	82.432	148	15	3	1	147	1	138	6.01e-71	210
MsG0480020919.01.T01	AT3G53650	81.208	149	16	3	1	148	1	138	4.61e-69	205
MsG0480020919.01.T01	AT5G22880	87.586	145	16	2	4	148	3	145	4.25e-67	200
MsG0480020919.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	9.71e-67	199
MsG0480020919.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.13e-62	187
MsG0480020919.01.T01	AT1G08170	61.176	85	33	0	61	145	151	235	2.97e-37	127

Find 43 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGTCAGACTTGTTCTTCC+TGG	0.316295	4:-50593968	MsG0480020919.01.T01:CDS
GGTAGCTTCTTCCCTGCCTT+TGG	0.322369	4:+50594235	None:intergenic
GCTGGCTTCTTCTCTCCCTT+TGG	0.330331	4:+50594322	None:intergenic
TTGCTTGAGATACCAATGTC+TGG	0.335174	4:+50594109	None:intergenic
ACTTGTTCTTCCTGGTGAAT+TGG	0.346831	4:-50593960	MsG0480020919.01.T01:CDS
GGCAGCGGAACCTCCTTCCT+TGG	0.364599	4:+50594213	None:intergenic
TCTGCGGGTTTCTTCTCTGC+TGG	0.399254	4:+50594304	None:intergenic
CTTCTTCTTGTCTCCGGCAG+CGG	0.399506	4:+50594198	None:intergenic
GCCTTTGGCTTCTTCTCCGC+CGG	0.402654	4:+50594250	None:intergenic
ATTTCCCTGGAAGTGATTGT+TGG	0.430207	4:+50593998	None:intergenic
GTGGATTTCTTCTCCTCTGC+GGG	0.433451	4:+50594289	None:intergenic
GCGGAGAAGAAGCCAAAGGC+AGG	0.445065	4:-50594247	MsG0480020919.01.T01:CDS
ATACAAGATCTACATCTTCA+AGG	0.451479	4:-50594149	MsG0480020919.01.T01:CDS
AGAAGCCAACAATCACTTCC+AGG	0.458850	4:-50594003	MsG0480020919.01.T01:CDS
CATCCTTTCAATGGCACCAA+AGG	0.468979	4:-50594338	None:intergenic
GCAGCGGAACCTCCTTCCTT+GGG	0.469567	4:+50594214	None:intergenic
TCTCTTCTTCTTCTTGTCTC+CGG	0.474508	4:+50594192	None:intergenic
AAGGAAGGAGGTTCCGCTGC+CGG	0.482012	4:-50594211	MsG0480020919.01.T01:CDS
GACAGCAGTCTGAATTTCCC+TGG	0.496873	4:+50593985	None:intergenic
ATCCTTTCAATGGCACCAAA+GGG	0.515703	4:-50594337	None:intergenic
CGCCGGAGCTTTCTCAGCGA+CGG	0.521178	4:+50594267	None:intergenic
TGCTTGAGATACCAATGTCT+GGG	0.532971	4:+50594110	None:intergenic
TGGTATCTCAAGCAAAGCCA+TGG	0.533917	4:-50594101	MsG0480020919.01.T01:CDS
GCTAAGCATGCCGTCTCTGA+GGG	0.544354	4:-50593938	MsG0480020919.01.T01:CDS
AGCCTTGGTGCCCTCAGAGA+CGG	0.547548	4:+50593928	None:intergenic
CACCGTCGCTGAGAAAGCTC+CGG	0.552253	4:-50594269	MsG0480020919.01.T01:CDS
CGGAGAAGAAGCCAAAGGCA+GGG	0.564014	4:-50594246	MsG0480020919.01.T01:CDS
GGGAAGAAGCTACCCAAGGA+AGG	0.585765	4:-50594226	MsG0480020919.01.T01:CDS
GGCTAAGCATGCCGTCTCTG+AGG	0.592749	4:-50593939	MsG0480020919.01.T01:CDS
AGTAAACTTAGTCACAGCCT+TGG	0.601904	4:+50593913	None:intergenic
GGCAGGGAAGAAGCTACCCA+AGG	0.607887	4:-50594230	MsG0480020919.01.T01:CDS
GCATGCTTAGCCAATTCACC+AGG	0.611062	4:+50593950	None:intergenic
TGCCGTCTCTGAGGGCACCA+AGG	0.612198	4:-50593930	MsG0480020919.01.T01:CDS
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:-50594121	MsG0480020919.01.T01:CDS
TCCGGCGGAGAAGAAGCCAA+AGG	0.620050	4:-50594251	MsG0480020919.01.T01:CDS
GAAGCCAACAATCACTTCCA+GGG	0.630002	4:-50594002	MsG0480020919.01.T01:CDS
GAAACTGTTCATGATTCCCA+TGG	0.633384	4:+50594084	None:intergenic
CGTCGCTGAGAAAGCTCCGG+CGG	0.640688	4:-50594266	MsG0480020919.01.T01:CDS
GGTGGATTTCTTCTCCTCTG+CGG	0.645875	4:+50594288	None:intergenic
AAGAAGCTACCCAAGGAAGG+AGG	0.646492	4:-50594223	MsG0480020919.01.T01:CDS
GGTATCTCAAGCAAAGCCAT+GGG	0.654333	4:-50594100	MsG0480020919.01.T01:CDS
CGGAGCTTTCTCAGCGACGG+TGG	0.663279	4:+50594270	None:intergenic
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4:-50594302	MsG0480020919.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATACAAGATCTACATCTTCA+AGG	-	Chr4:50594083-50594102	MsG0480020919.01.T01:CDS	30.0%
	AAACAAGTTCACCCAGACAT+TGG	-	Chr4:50594111-50594130	MsG0480020919.01.T01:CDS	40.0%
	ACTTGTTCTTCCTGGTGAAT+TGG	-	Chr4:50594272-50594291	MsG0480020919.01.T01:CDS	40.0%
	AGTAAACTTAGTCACAGCCT+TGG	+	Chr4:50594322-50594341	None:intergenic	40.0%
	ATTTCCCTGGAAGTGATTGT+TGG	+	Chr4:50594237-50594256	None:intergenic	40.0%
	GAAACTGTTCATGATTCCCA+TGG	+	Chr4:50594151-50594170	None:intergenic	40.0%
	TCTCTTCTTCTTCTTGTCTC+CGG	+	Chr4:50594043-50594062	None:intergenic	40.0%
	TGCTTGAGATACCAATGTCT+GGG	+	Chr4:50594125-50594144	None:intergenic	40.0%
	TTGCTTGAGATACCAATGTC+TGG	+	Chr4:50594126-50594145	None:intergenic	40.0%
	AGAAGCCAACAATCACTTCC+AGG	-	Chr4:50594229-50594248	MsG0480020919.01.T01:CDS	45.0%
	GAAGCCAACAATCACTTCCA+GGG	-	Chr4:50594230-50594249	MsG0480020919.01.T01:CDS	45.0%
	GGTATCTCAAGCAAAGCCAT+GGG	-	Chr4:50594132-50594151	MsG0480020919.01.T01:CDS	45.0%
	TGGTATCTCAAGCAAAGCCA+TGG	-	Chr4:50594131-50594150	MsG0480020919.01.T01:CDS	45.0%
	AAGAAGCTACCCAAGGAAGG+AGG	-	Chr4:50594009-50594028	MsG0480020919.01.T01:CDS	50.0%
	GACAGCAGTCTGAATTTCCC+TGG	+	Chr4:50594250-50594269	None:intergenic	50.0%
	GCATGCTTAGCCAATTCACC+AGG	+	Chr4:50594285-50594304	None:intergenic	50.0%
	GTGGATTTCTTCTCCTCTGC+GGG	+	Chr4:50593946-50593965	None:intergenic	50.0%
!	GCTGTCAGACTTGTTCTTCC+TGG	-	Chr4:50594264-50594283	MsG0480020919.01.T01:CDS	50.0%
!	GGTGGATTTCTTCTCCTCTG+CGG	+	Chr4:50593947-50593966	None:intergenic	50.0%
	AGCAGAGAAGAAACCCGCAG+AGG	-	Chr4:50593930-50593949	MsG0480020919.01.T01:CDS	55.0%
	CGGAGAAGAAGCCAAAGGCA+GGG	-	Chr4:50593986-50594005	MsG0480020919.01.T01:CDS	55.0%
	CTTCTTCTTGTCTCCGGCAG+CGG	+	Chr4:50594037-50594056	None:intergenic	55.0%
	GCTAAGCATGCCGTCTCTGA+GGG	-	Chr4:50594294-50594313	MsG0480020919.01.T01:CDS	55.0%
	GGGAAGAAGCTACCCAAGGA+AGG	-	Chr4:50594006-50594025	MsG0480020919.01.T01:CDS	55.0%
	GGTAGCTTCTTCCCTGCCTT+TGG	+	Chr4:50594000-50594019	None:intergenic	55.0%
	TCTGCGGGTTTCTTCTCTGC+TGG	+	Chr4:50593931-50593950	None:intergenic	55.0%
!	GCTGGCTTCTTCTCTCCCTT+TGG	+	Chr4:50593913-50593932	None:intergenic	55.0%
	AAGGAAGGAGGTTCCGCTGC+CGG	-	Chr4:50594021-50594040	MsG0480020919.01.T01:CDS	60.0%
	CACCGTCGCTGAGAAAGCTC+CGG	-	Chr4:50593963-50593982	MsG0480020919.01.T01:CDS	60.0%
	GCAGCGGAACCTCCTTCCTT+GGG	+	Chr4:50594021-50594040	None:intergenic	60.0%
	GCGGAGAAGAAGCCAAAGGC+AGG	-	Chr4:50593985-50594004	MsG0480020919.01.T01:CDS	60.0%
	GGCAGGGAAGAAGCTACCCA+AGG	-	Chr4:50594002-50594021	MsG0480020919.01.T01:CDS	60.0%
	GGCTAAGCATGCCGTCTCTG+AGG	-	Chr4:50594293-50594312	MsG0480020919.01.T01:CDS	60.0%
	TCCGGCGGAGAAGAAGCCAA+AGG	-	Chr4:50593981-50594000	MsG0480020919.01.T01:CDS	60.0%
!	GCCTTTGGCTTCTTCTCCGC+CGG	+	Chr4:50593985-50594004	None:intergenic	60.0%
!!	AGCCTTGGTGCCCTCAGAGA+CGG	+	Chr4:50594307-50594326	None:intergenic	60.0%
	CGCCGGAGCTTTCTCAGCGA+CGG	+	Chr4:50593968-50593987	None:intergenic	65.0%
	CGGAGCTTTCTCAGCGACGG+TGG	+	Chr4:50593965-50593984	None:intergenic	65.0%
	CGTCGCTGAGAAAGCTCCGG+CGG	-	Chr4:50593966-50593985	MsG0480020919.01.T01:CDS	65.0%
	GGCAGCGGAACCTCCTTCCT+TGG	+	Chr4:50594022-50594041	None:intergenic	65.0%
!!	TGCCGTCTCTGAGGGCACCA+AGG	-	Chr4:50594302-50594321	MsG0480020919.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	50593904	50594350	50593904	ID=MsG0480020919.01;Name=MsG0480020919.01
Chr4	mRNA	50593904	50594350	50593904	ID=MsG0480020919.01.T01;Parent=MsG0480020919.01;Name=MsG0480020919.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	50593904	50594350	50593904	ID=MsG0480020919.01.T01:exon:324;Parent=MsG0480020919.01.T01
Chr4	CDS	50593904	50594350	50593904	ID=MsG0480020919.01.T01:cds;Parent=MsG0480020919.01.T01

Gene Sequence

>MsG0480020919.01.T01

ATGGCACCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACCGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCAGGGAAGAAGCTACCCAAGGAAGGAGGTTCCGCTGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGTGTTGAAACATACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAATGATATCTTCGAGAAGCTTGCTCAAGAATCTTCAAGACTCGCTCGTTACAATAAGAAGCCAACAATCACTTCCAGGGAAATTCAGACTGCTGTCAGACTTGTTCTTCCTGGTGAATTGGCTAAGCATGCCGTCTCTGAGGGCACCAAGGCTGTGACTAAGTTTACTAGTTCTTAG

Protein sequence

>MsG0480020919.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*