AlfalfaGEDB | MsG0480020919.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020919.01

KEGG: K11252
GO: GO:0000786 GO:0003677 GO:0046982
PFAM: PF00125

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	XP_003606627.1	99.324	148	1	0	1	148	1	148	3.31E-98	289

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	Q1S9I9	99.324	148	1	0	1	148	1	148	8.77E-101	288

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	G7JTQ7	99.324	148	1	0	1	148	1	148	1.58e-98	289

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0480020919.01	MsG0480020920.01	0.832934	7.118598e-56	8.075213e-53
MsG0480020919.01	MsG0480020969.01	0.804792	1.822551e-49	9.483673e-47
MsG0480020919.01	MsG0480021015.01	0.809961	1.460951e-50	8.697249e-48
MsG0480020919.01	MsG0480021049.01	0.825553	4.403436e-54	4.026721e-51

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PPI

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Gene1	Gene2	Type
MsG0180000243.01	MsG0480020919.01	PPI
MsG0480020877.01	MsG0480020919.01	PPI
MsG0480020919.01	MsG0880045015.01	PPI
MsG0480020919.01	MsG0480023842.01	PPI
MsG0480020919.01	MsG0880047260.01	PPI
MsG0480020919.01	MsG0480022307.01	PPI
MsG0480020919.01	MsG0280008380.01	PPI
MsG0480020919.01	MsG0580025504.01	PPI
MsG0480020919.01	MsG0780036534.01	PPI
MsG0480020919.01	MsG0780041592.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	MTR_4g063220	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g063990	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g063200	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g064020	99.324	148	1	0	1	148	1	148	4.00e-102	289
MsG0480020919.01.T01	MTR_4g071180	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480020919.01.T01	MTR_4g070240	99.324	148	1	0	1	148	1	148	8.54e-102	288
MsG0480020919.01.T01	MTR_4g070245	97.973	148	3	0	1	148	1	148	4.20e-100	284
MsG0480020919.01.T01	MTR_4g063240	98.649	148	1	1	1	148	1	147	1.15e-99	283
MsG0480020919.01.T01	MTR_4g063275	96.622	148	5	0	1	148	1	148	8.49e-99	280
MsG0480020919.01.T01	MTR_4g064983	98.611	144	2	0	1	144	1	144	8.23e-97	279

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020919.01.T01	AT3G45980	88.742	151	13	2	1	148	1	150	1.47e-83	242
MsG0480020919.01.T01	AT2G28720	86.093	151	18	1	1	148	1	151	9.69e-81	235
MsG0480020919.01.T01	AT1G07790	86.928	153	10	3	1	148	1	148	7.69e-80	233
MsG0480020919.01.T01	AT5G59910	91.176	136	9	2	13	148	18	150	2.65e-77	226
MsG0480020919.01.T01	AT3G46030	94.444	126	6	1	23	148	21	145	8.66e-76	222
MsG0480020919.01.T01	AT2G37470	82.432	148	15	3	1	147	1	138	6.01e-71	210
MsG0480020919.01.T01	AT3G53650	81.208	149	16	3	1	148	1	138	4.61e-69	205
MsG0480020919.01.T01	AT5G22880	87.586	145	16	2	4	148	3	145	4.25e-67	200
MsG0480020919.01.T01	AT5G02570	79.730	148	14	1	1	148	1	132	9.71e-67	199
MsG0480020919.01.T01	AT3G09480	84.127	126	16	1	23	148	5	126	2.13e-62	187

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Find 43 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAAGTTCACCCAGACAT+TGG	0.612279	4:-50594121	MsG0480020919.01.T01:CDS
AAGAAGCTACCCAAGGAAGG+AGG	0.646492	4:-50594223	MsG0480020919.01.T01:CDS
AAGGAAGGAGGTTCCGCTGC+CGG	0.482012	4:-50594211	MsG0480020919.01.T01:CDS
ACTTGTTCTTCCTGGTGAAT+TGG	0.346831	4:-50593960	MsG0480020919.01.T01:CDS
AGAAGCCAACAATCACTTCC+AGG	0.458850	4:-50594003	MsG0480020919.01.T01:CDS
AGCAGAGAAGAAACCCGCAG+AGG	0.698236	4:-50594302	MsG0480020919.01.T01:CDS
AGCCTTGGTGCCCTCAGAGA+CGG	0.547548	4:+50593928	None:intergenic
AGTAAACTTAGTCACAGCCT+TGG	0.601904	4:+50593913	None:intergenic
ATACAAGATCTACATCTTCA+AGG	0.451479	4:-50594149	MsG0480020919.01.T01:CDS
ATCCTTTCAATGGCACCAAA+GGG	0.515703	4:-50594337	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACAAGATCTACATCTTCA+AGG	-	Chr4:50594083-50594102	MsG0480020919.01.T01:CDS	30.0%
AAACAAGTTCACCCAGACAT+TGG	-	Chr4:50594111-50594130	MsG0480020919.01.T01:CDS	40.0%
ACTTGTTCTTCCTGGTGAAT+TGG	-	Chr4:50594272-50594291	MsG0480020919.01.T01:CDS	40.0%
AGTAAACTTAGTCACAGCCT+TGG	+	Chr4:50594322-50594341	None:intergenic	40.0%
ATTTCCCTGGAAGTGATTGT+TGG	+	Chr4:50594237-50594256	None:intergenic	40.0%
GAAACTGTTCATGATTCCCA+TGG	+	Chr4:50594151-50594170	None:intergenic	40.0%
TCTCTTCTTCTTCTTGTCTC+CGG	+	Chr4:50594043-50594062	None:intergenic	40.0%
TGCTTGAGATACCAATGTCT+GGG	+	Chr4:50594125-50594144	None:intergenic	40.0%
TTGCTTGAGATACCAATGTC+TGG	+	Chr4:50594126-50594145	None:intergenic	40.0%
AGAAGCCAACAATCACTTCC+AGG	-	Chr4:50594229-50594248	MsG0480020919.01.T01:CDS	45.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	50593904	50594350	50593904	ID=MsG0480020919.01;Name=MsG0480020919.01
Chr4	mRNA	50593904	50594350	50593904	ID=MsG0480020919.01.T01;Parent=MsG0480020919.01;Name=MsG0480020919.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|148
Chr4	exon	50593904	50594350	50593904	ID=MsG0480020919.01.T01:exon:324;Parent=MsG0480020919.01.T01
Chr4	CDS	50593904	50594350	50593904	ID=MsG0480020919.01.T01:cds;Parent=MsG0480020919.01.T01

Gene Sequence

>MsG0480020919.01.T01

ATGGCACCAAAGGGAGAGAAGAAGCCAGCAGAGAAGAAACCCGCAGAGGAGAAGAAATCCACCGTCGCTGAGAAAGCTCCGGCGGAGAAGAAGCCAAAGGCAGGGAAGAAGCTACCCAAGGAAGGAGGTTCCGCTGCCGGAGACAAGAAGAAGAAGAGAAGCAAGAAGAGTGTTGAAACATACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCACCCAGACATTGGTATCTCAAGCAAAGCCATGGGAATCATGAACAGTTTCATCAATGATATCTTCGAGAAGCTTGCTCAAGAATCTTCAAGACTCGCTCGTTACAATAAGAAGCCAACAATCACTTCCAGGGAAATTCAGACTGCTGTCAGACTTGTTCTTCCTGGTGAATTGGCTAAGCATGCCGTCTCTGAGGGCACCAAGGCTGTGACTAAGTTTACTAGTTCTTAG

Protein sequence

>MsG0480020919.01.T01

MAPKGEKKPAEKKPAEEKKSTVAEKAPAEKKPKAGKKLPKEGGSAAGDKKKKRSKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*